Classifications

• • C—CHEMISTRY; METALLURGY
  • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
  • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
  • C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
  • C11D1/38—Cationic compounds
  • C11D1/645—Mixtures of compounds all of which are cationic
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
  • A01N33/02—Amines; Quaternary ammonium compounds
  • A01N33/12—Quaternary ammonium compounds
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
  • A01N37/14—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group; Thio analogues thereof
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
  • A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
• • C—CHEMISTRY; METALLURGY
  • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
  • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
  • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
  • C11D3/16—Organic compounds
  • C11D3/20—Organic compounds containing oxygen
  • C11D3/2075—Carboxylic acids-salts thereof
• • C—CHEMISTRY; METALLURGY
  • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
  • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
  • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
  • C11D3/39—Organic or inorganic per-compounds
  • C11D3/3947—Liquid compositions
• • C—CHEMISTRY; METALLURGY
  • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
  • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
  • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
  • C11D3/48—Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions
• • C—CHEMISTRY; METALLURGY
  • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
  • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
  • C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
  • C11D1/38—Cationic compounds
  • C11D1/42—Amino alcohols or amino ethers
  • C11D1/44—Ethers of polyoxyalkylenes with amino alcohols; Condensation products of epoxyalkanes with amines
• • C—CHEMISTRY; METALLURGY
  • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
  • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
  • C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
  • C11D1/38—Cationic compounds
  • C11D1/62—Quaternary ammonium compounds
• • C—CHEMISTRY; METALLURGY
  • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
  • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
  • C11D2111/00—Cleaning compositions characterised by the objects to be cleaned; Cleaning compositions characterised by non-standard cleaning or washing processes
  • C11D2111/10—Objects to be cleaned
  • C11D2111/12—Soft surfaces, e.g. textile
• • C—CHEMISTRY; METALLURGY
  • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
  • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
  • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
  • C11D3/16—Organic compounds
  • C11D3/20—Organic compounds containing oxygen
  • C11D3/2075—Carboxylic acids-salts thereof
  • C11D3/2086—Hydroxy carboxylic acids-salts thereof

Definitions

• the present invention relates to an aqueous laundry treatment composition.
• the present invention relates to an aqueous laundry treatment composition for removing germs from a fabric.
• Washing fabrics also known as laundering or simply as laundry, makes up one of the main chores that people undertake routinely. For this, people have been relying on detergent compositions that are widely available.
• a fabric is washed by contacting it with a detergent composition either in neat or diluted form, rinsing the fabric one or more times e.g. thrice, with water; and finally, drying it.
• a fabric is cleaned by mechanically agitating it using hands and/or by using suitable cleaning means e.g. a brush, in so-called hand-wash method.
• a fabric is cleaned by mechanically agitations carried out by a machine.
• a step of soaking a fabric, in a neat or diluted detergent composition may be carried out for a desired duration e.g. 30 to 60 minutes.
• the step of drying may also be carried out by using machines; or simply by exposing the fabric to air.
• surfactants contained in a detergent composition
• a detergent composition are primarily used for the cleaning function they offer.
• surfactants alone or along with other ingredients that may be present in a detergent composition are able to remove germs that may be adhering to a fabric. This may be simply due to a possibility that surfactants interact chemically with germs and/or due to mechanical agitation caused during the cleaning and rinsing steps.
• a detergent composition used and/or the mechanical agitation may not necessarily provide optimal germ removal. As a result, germs may still remain adhered to a fabric in an amount enough to cause one or more problems mentioned above.
• an aqueous laundry treatment composition comprising one or more quaternary ammonium compound in combination with one or more organic acid in certain pKa range, a peroxide and an ethoxylated amine, provides optimal germ removal from a fabric.
• the present invention relates to an aqueous laundry treatment composition
• an aqueous laundry treatment composition comprising: a. from 0.5 to 5 wt% of one or more quaternary ammonium compound, b. from 0.1 to 5 wt% of one or more organic acid having pKa in the range from 1 to 5.5, c. from 1 to 5 wt% of a peroxide; and d. from 0.5 to 5 wt% ethoxylated amine with saturated or unsaturated alkyl groups having carbon atoms in the range from 12 to 22; and 1 to 50 ethoxylate groups.
• the present invention relates to a method of removing germs from a fabric by a. contacting the fabric with the composition of the first aspect for 5 to 60 minutes, b. rinsing it with water; and c. drying the fabric.
• the present invention relates to use of the composition of the first aspect for removing germs from a fabric.
• ‘treatment’ as used in ‘a laundry treatment composition’ preferably means an action carried out on fabrics that is in addition to the washing step carried out using a detergent composition. Accordingly, a treatment may be carried out before, during or after the washing step is over. Preferably, the treatment is carried out after the washing step is over, i.e. after the fabrics are cleaned, with a detergent composition.
• washed fabric may simply be contacted with a laundry treatment composition as per the present invention (the composition).
• the composition may simply be added to a compartment e.g. fabric conditioner dispensing compartment, beforehand.
• the composition is in addition to a detergent composition that may be used in the washing step.
• a detergent composition preferably means a composition that comprises surfactants; and is meant to be used in the washing step; and is different and separate from the composition.
• phrases indicating ‘removal of germs’ from a fabric may be used interchangeably with ‘sanitizing the laundry’, ‘sanitizing a fabric’, ‘imparting hygiene to a fabric’; and all these phrases denote the same meaning.
• the present invention relates to an aqueous laundry treatment composition comprising: a. from 0.5 to 5 wt% of one or more quaternary ammonium compound, b.
• Quaternary ammonium compound The composition comprises a quaternary ammonium compound.
• the quaternary ammonium compound present in the composition acts as a germicide.
• the quaternary ammonium compound is selected from the group consisting of didecyl dimethyl ammonium chloride, dioctyl dimethyl ammonium chloride, alkyl dimethyl benzyl ammonium chloride, diisobutyl phenoxy ethoxy ethyl dimethyl benzyl ammonium chloride, alkyl dimethyl benzyl ammonium saccharinate, octyl decyl dimethyl ammonium chloride, alkyl dimethyl ethyl benzyl ammonium chloride, methyldodecylbenzyl ammonium chloride, methyldodecylxylene-bis-trimethyl ammonium chloride, methyl benzethonium chloride, cetyl pyrinidinium chloride, cetrimonium bromide; and mixtures thereof.
• the quaternary ammonium compound is selected from alkyl dimethyl benzyl ammonium chloride e.g. benzalkonium chloride (BKC), diisobutyl phenoxy ethoxy ethyl dimethyl benzyl ammonium chloride (benzethonium chloride; BZC), methyl benzethonium chloride, cetyl pyrinidinium chloride, cetrimonium bromide; and mixtures thereof.
• BKC benzalkonium chloride
• BZC diisobutyl phenoxy ethoxy ethyl dimethyl benzyl ammonium chloride
• methyl benzethonium chloride cetyl pyrinidinium chloride, cetrimonium bromide
• the quaternary ammonium compound is benzalkonium chloride.
• quaternary ammonium compounds mentioned above are available as a single quaternary ammonium compound, as well as mixtures of two or more different quaternary ammonium compounds e.g. under trademarks BARDACTM, BARQUAT ® and HYAMINE ® (all by Lonza); and BTC ® and ONYXIDE ® (both by Stepan).
• Quaternary ammonium compounds available as a single quaternary ammonium compound include didecyl dimethyl ammonium chloride (available as BARDACTM 2250 R and BTC ® 1010, both 50% active; and BARDACTM 2280 R and BTC ® 1010-80, both 80% active), dioctyl dimethyl ammonium chloride (available as BARDACTM LF, 50% active; BARDACTM LF-80, 80% active), alkyl dimethyl benzyl ammonium chloride (available as BARQUAT ® MB-50, BARQUAT ® MX-50, BARQUAT ® OJ-50, HYAMINE ® 3500, BTC ® 50, BTC ® 50E, BTC ® 65, BTC ® 776, BTC ® 824, BTC ® 835; each 50% active; and the same is available as BARQUAT ® MB-80, BARQUAT ® MX-80, HYAMINE ® 3500-
• Quaternary ammonium compounds that are available as a mixture of two or more quaternary ammonium compounds include a mixture of alkyl dimethyl benzyl ammonium chloride, octyl decyl dimethyl ammonium chloride, didecyl dimethyl ammonium chloride; and dioctyl dimethyl ammonium chloride (available as BARDACTM 205M, 50% active; and available as BARDACTM 208M, 80% active), a mixture of octyl decyl dimethyl ammonium chloride, didecyl dimethyl ammonium chloride; and dioctyl dimethyl ammonium chloride (available as BARDACTM 2050, 50% active; and available as BARDACTM 2080, 80% active), a mixture of alkyl dimethyl benzyl ammonium chloride and alkyl dimethyl ethyl benzyl ammonium chloride (available as BARQUAT ® 4250 and BARQUAT ® 4
• the composition comprises from 0.5 to 5 wt%, preferably from 0.75 to 4.5 wt%, more preferably from 1 to 4 wt%, even more preferably from 1.5 to 3.5 wt% further more preferably from 2 to 3 wt% of one or more quaternary ammonium compound.
• the composition comprises one or more organic acid having pKa in the range from 1 to 5.5.
• the one or more organic acid selected are such that they are stable in the composition comprising peroxide.
• such organic acids are selected from citric acid, lactic acid and mixtures thereof. Most preferably, the organic acid is citric acid.
• the composition comprises from 0.1 to 5 wt%, preferably from 0.5 to 4.5 wt%, more preferably from 1 to 4 wt%, even more preferably from 1.5 to 3.5 wt%, further more preferably from 2 to 3 wt% of one or more organic acid.
• the composition comprises a peroxide.
• the peroxide is selected from the group consisting of hydrogen peroxide (H2O2), peracetic acid, barium peroxide (BaC>2), sodium peroxide (Na2C>2), tert-butylhydroperoxide, potassium peroxydisulfate.
• the peroxide selected is hydrogen peroxide.
• one or more sources of hydrogen peroxide selected from sodium percarbonate, sodium perborate, sodium persilicate, sodium persulphate; which release hydrogen peroxide when contacted with water may also be used preferably in combination with an activator e.g. tetra-acetylethylenediamine (TAED).
• TAED tetra-acetylethylenediamine
• the composition comprises from 1 to 5 wt%, preferably from 1 to 4 wt%, more preferably from 1 to 3 wt%, even more preferably from 1 to 2 wt% of a peroxide.
• the composition comprises ethoxylated amine with saturated or unsaturated alkyl groups having carbon atoms in the range from 12 to 22; and 1 to 50 ethoxylate (EO) groups (ethoxylated amine).
• EO ethoxylate
• the ethoxylated amine comprises from 1 to 50, preferably from 1 to 30, more preferably from 1 to 15, even more preferably from 1 to 10, further more preferably from 1 to 7, still more preferably 1 to 5 and most preferably 1 to 2 EO groups.
• a general structure of ethoxylated amine is as follows:
• R is the alkyl chain
• number of carbon atom and degree of unsaturation in alkyl chain vary depending on type of ethoxylated amine
• m and n represent the number of ethoxylate present in ethoxylated amine.
• ethoxylated amine examples include cocoamine ethoxylates, oleyl amine ethoxylates, tallow amine ethoxylates; and their blends too are suitable to be used for this application.
• saturated or unsaturated alkyl groups having carbon atoms in the range from 12 to 22 it will be understood that the alkyl chain attached to the ethoxylated amine may also contain a mixture of alkyl groups e.g. C12, C14, C16, C18, C20 and C22 in varying proportions; and they may be saturated or unsaturated.
• Suitable ethoxylated amine may or may not be hydrogenated. Preferably, the ethoxylated amine is not hydrogenated.
• ethoxylated amine is selected from the group consisting of Bis(2- hydroxyethyl) coco alkylamine (commercially available as Ethomeen C/12; from
• Ethoxylated (7) Cocoalkylamine (commercially available as Ethomeen C/17 (from Nouryon), Polyoxyethylene (15) tallowalkylamines (commercially available as Ethomeen T/25 (from Nouryon), Hydrogenated Tallow Amine 50 EO (commercially available as Ethomeen HT/60 (from Nouryon) and combinations thereof. More preferably, the ethoxylated amine that may be present in the composition is PEG-2- oleyl amine commercially available as Ethomeen 0/12 (from Nouyron).
• the composition comprises from 0.5 to 5 wt%, preferably from 0.75 to 4 wt%, more preferably from 0.75 to 3 wt%, even more preferably from 0.75 to 2.5 wt%, further more preferably from 0.75 to 2 wt%, still more preferably 0.75 to 1.5 wt% and yet more preferably from 0.75 to 1 wt% ethoxylated amine. pH of the composition
• the composition has pH in the range from 1.0 to 6.5, more preferably from 1.5 to 6.0, even more preferably from 2.0 to 5.5, further more preferably from 2.0 to 5.0, still more preferably from 2.0 to 4.5, yet more preferably from 2.0 to 4.0, still further more preferably from 2.0 to 3.5, yet further more preferably from 2.0 to 3.0 and yet still further more preferably from 2.0 to 2.5.
• the pH of the composition may be adjusted using suitable pH adjusting agents e.g. hydrochloric acid and sodium hydroxide.
• the composition comprises water in an amount ranging preferably from 50 to 98 wt%, more preferably from 60 to 97 wt%, even more preferably from 70 to 96 wt%, further more preferably from 70 to 94 wt%, still more preferably from 70 to 93 wt%, yet more preferably from 75 to 92 wt%, still further more preferably from 75 to 91 and yet further more preferably 80 to 90 wt% water.
• the composition is preferably an aqueous laundry treatment composition comprising: a. from 0.5 to 5 wt% of one or more quaternary ammonium compound, b.
• the composition is an aqueous laundry treatment composition
• the composition further comprises one or more sequestrants.
• a preferred sequestrant is a phosphonic acid or a salt thereof.
• the phosphonic acid (or salt thereof) sequestrant is preferably selected from the group consisting of 1 -Hydroxyethylidene- 1,1-diphosphonic acid (HEDP; commercially available as Dequest® 2010), Diethylenetriaminepenta(methylenephosphonic acid) (DTPMP; commercially available as Dequest® 2066), Hexamethylenediaminetetra(methylenephosphonic acid)
• HEDP 1 -Hydroxyethylidene- 1,1-diphosphonic acid
• DTPMP Diethylenetriaminepenta(methylenephosphonic acid)
• Dequest® 2066 Hexamethylenediaminetetra(methylenephosphonic acid
• the sequestrant selected is HEDP.
• the composition comprises sequestrant that may be present in an amount from 0.1 to 5 wt%, more preferably from 0.25 to 4 wt%, even more preferably from 0.5 to 2.5 wt%.
• Viscosity of the composition is measured using an AR 1000 Rheometer (TA instruments) using a 4 cm, 2° cone-plate geometry at 25°C.
• the composition has viscosity less than 10 mPa.s @ 20 s -1 . More preferably, the composition as viscosity in the range from 1 to a maximum of 10 mPa.s @ 20 s -1 .
• the composition is substantially less viscous.
• the composition does not contain any viscosity modifiers.
• the composition may be used as is, i.e. neat, preferably the composition is diluted with water before use. More preferably, the composition is diluted before use. For example, if applied in a diluted form, the composition is diluted with water preferably in a ratio 1:1000, more preferably 1:500, even more preferably 1:200 and further more preferably 1:100 which is substantially more diluted than a typical household cleaning composition e.g. a toilet cleaning composition, which tend to have dilutions e.g. 1:40 when used.
• composition may optionally comprise other ingredients, such as fragrance.
• the present invention relates to a method of removing germs from a fabric by a. contacting the fabric with the composition of the first aspect for 5 to 60 minutes, b. rinsing it with water; and c. drying the fabric.
• a fabric is contacted with the composition of the first aspect for 5 to 60 minutes, preferably for 5 to 50 minutes, more preferably for 10 to 40 minutes, further more preferably for 10 to 30 minutes and still more preferably for 10 to 20 minutes.
• step a is carried out after a fabric is washed with a detergent composition.
• a detergent composition for example, washed fabrics may be treated with the composition where 50 ml_ of the composition is added to 5000ml_ of water for 10 minutes before rinsing step (step b) is carried out as described below.
• step a is carried out at water temperature e. g. by using water which is at 25°C.
• the composition is added to a compartment of a washing machine e.g. a compartment of dispensing fabric conditioner, at the start of the washing machine cycle; and carrying out the wash cycle as usual.
• a temperature e.g. 30°C or 40°C, that may be set in a machine.
• Step b the rinsing step, is carried out preferably for once, more preferably twice or thrice, by rinsing the fabric with water.
• step b is carried out at water temperature or at a temperature that may be set in a machine (as described above)
• Step c is carried out either using a machine drying or simply exposing the fabric to air, until it is dry.
• step c is carried out at an ambient temperature, alternatively; step c may be carried out at elevated temperatures e.g. 40°C or 50°C, that may be set in a washing machine or a drying machine.
• germs are removed from a fabric.
• a fabric is treated using the composition; and according to the method of the second aspect, germs are removed from a fabric.
• germs are removed from a fabric.
• a fabric to be treated using the composition include clothes, apparels, face masks, overcoats and gloves.
• the present invention relates to use of the composition according to the first aspect for removing germs from a fabric.
• the use according to the third aspect involves contacting a fabric with the composition of the first aspect either in neat or preferably in diluted form, for 5 to 60 minutes.
• the composition preferably more than 90%, more preferably more than 95% and even more preferably more than 99.9% germs are removed from a fabric.
• Germ removal efficacy of the composition along with a control composition shown in table 1 below were carried out using EN 1276 protocol for estimating antimicrobial efficacy. Soil conditions clean were created with 0.03% Bovine Serum Albumin (BSA); and dirty conditions were created by using 0.3% BSA with a contact time of 5 min.
• BSA Bovine Serum Albumin
• Differential media was used for enumerating the test organisms (to eliminate the growth of Bacillus along with the test organism).
• a saline suspension of the above-mentioned test organisms is made.
• the optical density of the saline suspension is adjusted at 620 nm to obtain 10 8 cells/ ml_.
• All glycerol stocks of bacteria were maintained at -80°C; and were revived just before the carrying out the experiments.
• Dilution neutralization method used was as follows: a. To 8 ml of highest test concentration of test product dilution, 1 ml of saline + 1 ml of BSA (clean or dirty) is added, b. After 5 minutes, transfer 1 ml of above mixture to tube containing 8 ml of neutralizing broth to be validated, c. Add 1 ml of 10 5 dilution i. e 10 3 cells of test organism suspension (diluted from 10 8 cells/ ml test culture suspension in sterile saline) to the above tube and wait for 30 minutes. Consider this tube as undiluted test system; and d. After 30 minutes, plate out undiluted test system and -1 dilution.
• N is between 1.5 c 10 8 and 5.0 c 10 8 , (8,17 £ Ig N £ 8.70);
• N v0 is between 30 and 160, (3.0 c 10 1 and 1.6 c 10 2 );
• N v is between 3.0 c 10 2 and 1.6 c 10 3 ); c) A, B, C are equal to or greater than 0.5 c N v o ;
• N is the culture control count
• N v o is the neutralization validation culture control count
• C is the neutralization validation count obtained for test sample.
• Log reduction is calculated as difference of log of culture control in test mixture from log of count obtained in test mixture.
• Example A control and example 1 (the compositions according to the present invention)
• Germ removal data obtained for the control composition (example A) and composition as per the present invention (example 1) as estimated using EN1276 protocol cited hereinabove was found to be as follows:

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• Life Sciences & Earth Sciences (AREA)
• Chemical & Material Sciences (AREA)
• Wood Science & Technology (AREA)
• Engineering & Computer Science (AREA)
• Health & Medical Sciences (AREA)
• Dentistry (AREA)
• Environmental Sciences (AREA)
• Pest Control & Pesticides (AREA)
• Organic Chemistry (AREA)
• General Health & Medical Sciences (AREA)
• Agronomy & Crop Science (AREA)
• Zoology (AREA)
• Plant Pathology (AREA)
• Oil, Petroleum & Natural Gas (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Inorganic Chemistry (AREA)
• Emergency Medicine (AREA)
• Detergent Compositions (AREA)
• Treatments For Attaching Organic Compounds To Fibrous Goods (AREA)

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• 2021
  • 2021-04-12 PH PH1/2022/552369A patent/PH12022552369A1/en unknown
  • 2021-04-12 EP EP21717454.9A patent/EP4143286A1/de not_active Withdrawn
  • 2021-04-12 CN CN202180025578.0A patent/CN115362247A/zh active Pending
  • 2021-04-12 BR BR112022020313A patent/BR112022020313A2/pt unknown
  • 2021-04-12 WO PCT/EP2021/059388 patent/WO2021219351A1/en not_active Ceased
• 2022
  • 2022-09-13 ZA ZA2022/10162A patent/ZA202210162B/en unknown

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