EP4172342A4 - Entfernbare transgene pflanzenloci mit verwandten rna-führungserkennungsstellen - Google Patents
Entfernbare transgene pflanzenloci mit verwandten rna-führungserkennungsstellen Download PDFInfo
- Publication number
- EP4172342A4 EP4172342A4 EP21849192.6A EP21849192A EP4172342A4 EP 4172342 A4 EP4172342 A4 EP 4172342A4 EP 21849192 A EP21849192 A EP 21849192A EP 4172342 A4 EP4172342 A4 EP 4172342A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- recognition sites
- rna recognition
- plant transgenic
- guided rna
- transgenic loci
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000009261 transgenic effect Effects 0.000 title 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (18)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202063059916P | 2020-07-31 | 2020-07-31 | |
| US202063059963P | 2020-07-31 | 2020-07-31 | |
| US202063059860P | 2020-07-31 | 2020-07-31 | |
| US202063059813P | 2020-07-31 | 2020-07-31 | |
| US202163199930P | 2021-02-03 | 2021-02-03 | |
| US202163199951P | 2021-02-04 | 2021-02-04 | |
| US202163199949P | 2021-02-04 | 2021-02-04 | |
| US17/248,936 US11359210B2 (en) | 2020-07-31 | 2021-02-12 | INIR12 transgenic maize |
| US17/249,640 US11214811B1 (en) | 2020-07-31 | 2021-03-08 | INIR6 transgenic maize |
| US202163201030P | 2021-04-09 | 2021-04-09 | |
| US202163201029P | 2021-04-09 | 2021-04-09 | |
| US17/302,121 US11242534B1 (en) | 2020-07-31 | 2021-04-23 | INHT31 transgenic soybean |
| US17/302,110 US20220030822A1 (en) | 2020-07-31 | 2021-04-23 | Inht26 transgenic soybean |
| US17/302,739 US11326177B2 (en) | 2020-07-31 | 2021-05-11 | INIR12 transgenic maize |
| US17/303,116 US11369073B2 (en) | 2020-07-31 | 2021-05-20 | INIR12 transgenic maize |
| US202163202569P | 2021-06-16 | 2021-06-16 | |
| US202163203137P | 2021-07-09 | 2021-07-09 | |
| PCT/US2021/043207 WO2022026403A2 (en) | 2020-07-31 | 2021-07-26 | Removable plant transgenic loci with cognate guide rna recognition sites |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EP4172342A2 EP4172342A2 (de) | 2023-05-03 |
| EP4172342A4 true EP4172342A4 (de) | 2024-10-02 |
Family
ID=85128636
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP21849192.6A Pending EP4172342A4 (de) | 2020-07-31 | 2021-07-26 | Entfernbare transgene pflanzenloci mit verwandten rna-führungserkennungsstellen |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP4172342A4 (de) |
| BR (1) | BR112023001774A2 (de) |
| CA (1) | CA3188406A1 (de) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001066780A2 (en) * | 2000-03-09 | 2001-09-13 | Dekalb Genetics Corporation | Homologous recombination-mediated transgene alterations in plants |
| WO2002012517A1 (en) * | 2000-08-04 | 2002-02-14 | University Of Victoria Innovation And Development Corporation | Plant promoter derived from luminal binding protein gene and methods for its use |
| WO2006003186A1 (en) * | 2004-07-06 | 2006-01-12 | Biogemma | Method for enhancing gene expression in plants |
| WO2013116700A1 (en) * | 2012-02-01 | 2013-08-08 | Dow Agrosciences Llc | Glyphosate resistant plants and associated methods |
| WO2015118640A1 (ja) * | 2014-02-06 | 2015-08-13 | 日本たばこ産業株式会社 | 植物の形質転換細胞の取得方法 |
| WO2019018534A1 (en) * | 2017-07-18 | 2019-01-24 | The Board Of Trustees Of Leland Stanford Junior University | GENOME EDITION WITHOUT SCARCE THROUGH TWO-STEP APPROVED REPAIR BY HOMOLOGY |
-
2021
- 2021-07-26 EP EP21849192.6A patent/EP4172342A4/de active Pending
- 2021-07-26 CA CA3188406A patent/CA3188406A1/en active Pending
- 2021-07-26 BR BR112023001774A patent/BR112023001774A2/pt unknown
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001066780A2 (en) * | 2000-03-09 | 2001-09-13 | Dekalb Genetics Corporation | Homologous recombination-mediated transgene alterations in plants |
| WO2002012517A1 (en) * | 2000-08-04 | 2002-02-14 | University Of Victoria Innovation And Development Corporation | Plant promoter derived from luminal binding protein gene and methods for its use |
| WO2006003186A1 (en) * | 2004-07-06 | 2006-01-12 | Biogemma | Method for enhancing gene expression in plants |
| WO2013116700A1 (en) * | 2012-02-01 | 2013-08-08 | Dow Agrosciences Llc | Glyphosate resistant plants and associated methods |
| WO2015118640A1 (ja) * | 2014-02-06 | 2015-08-13 | 日本たばこ産業株式会社 | 植物の形質転換細胞の取得方法 |
| WO2019018534A1 (en) * | 2017-07-18 | 2019-01-24 | The Board Of Trustees Of Leland Stanford Junior University | GENOME EDITION WITHOUT SCARCE THROUGH TWO-STEP APPROVED REPAIR BY HOMOLOGY |
Non-Patent Citations (5)
| Title |
|---|
| ALI ZAHIR ET AL: "Fusion of the Cas9 endonuclease and the VirD2 relaxase facilitates homology-directed repair for precise genome engineering in rice", COMMUNICATIONS BIOLOGY, vol. 3, no. 1, 23 January 2020 (2020-01-23), pages 1 - 13, XP055910384, Retrieved from the Internet <URL:http://www.nature.com/articles/s42003-020-0768-9> DOI: 10.1038/s42003-020-0768-9 * |
| CAI ZHENKUN ET AL: "Broadening the targetable space: engineering and discovery of PAM-flexible Cas proteins", TRENDS IN MICROBIOLOGY, 1 May 2024 (2024-05-01), GB, XP093192173, ISSN: 0966-842X, DOI: 10.1016/j.tim.2024.05.004 * |
| CHRISTIAN MICHELLE ET AL: "Targeted mutagenesis of Arabidopsis thaliana using engineered TAL effector nucleases", G3 (BETHESDA, MD.), 3 October 2013 (2013-10-03), United States, pages 1697 - 1705, XP055902232, Retrieved from the Internet <URL:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3789794/pdf/1697.pdf> [retrieved on 20220316], DOI: 10.1534/g3.113.007104 * |
| SRIVASTAVA VIBHA ET AL: "Dual-targeting by CRISPR/Cas9 for precise excision of transgenes from rice genome", PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC), SPRINGER NETHERLANDS, DORDRECHT, vol. 129, no. 1, 20 January 2017 (2017-01-20), pages 153 - 160, XP036194323, ISSN: 0167-6857, [retrieved on 20170120], DOI: 10.1007/S11240-016-1166-3 * |
| WANG JUNJIE ET AL: "Generation of marker-free transgenic rice using CRISPR/Cas9 system controlled by floral specific promoters", JOURNAL OF GENETICS AND GENOMICS, vol. 46, no. 1, 2019, pages 61 - 64, XP085616545, ISSN: 1673-8527, DOI: 10.1016/J.JGG.2018.11.005 * |
Also Published As
| Publication number | Publication date |
|---|---|
| EP4172342A2 (de) | 2023-05-03 |
| CA3188406A1 (en) | 2022-02-03 |
| BR112023001774A2 (pt) | 2023-03-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE |
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| PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
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| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE |
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| 17P | Request for examination filed |
Effective date: 20230130 |
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| AK | Designated contracting states |
Kind code of ref document: A2 Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR |
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| DAV | Request for validation of the european patent (deleted) | ||
| DAX | Request for extension of the european patent (deleted) | ||
| A4 | Supplementary search report drawn up and despatched |
Effective date: 20240830 |
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| RIC1 | Information provided on ipc code assigned before grant |
Ipc: C12N 9/22 20060101ALI20240826BHEP Ipc: C12N 15/10 20060101ALI20240826BHEP Ipc: A01H 1/06 20060101ALI20240826BHEP Ipc: A01H 1/04 20060101ALI20240826BHEP Ipc: C12N 15/82 20060101AFI20240826BHEP |