EP4172342A4 - Entfernbare transgene pflanzenloci mit verwandten rna-führungserkennungsstellen - Google Patents

Entfernbare transgene pflanzenloci mit verwandten rna-führungserkennungsstellen Download PDF

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Publication number
EP4172342A4
EP4172342A4 EP21849192.6A EP21849192A EP4172342A4 EP 4172342 A4 EP4172342 A4 EP 4172342A4 EP 21849192 A EP21849192 A EP 21849192A EP 4172342 A4 EP4172342 A4 EP 4172342A4
Authority
EP
European Patent Office
Prior art keywords
recognition sites
rna recognition
plant transgenic
guided rna
transgenic loci
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP21849192.6A
Other languages
English (en)
French (fr)
Other versions
EP4172342A2 (de
Inventor
Michael Andreas KOCK
Joshua L. Price
Michael Lee NUCCIO
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Inari Agriculture Technology Inc
Original Assignee
Inari Agriculture Technology Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US17/248,936 external-priority patent/US11359210B2/en
Priority claimed from US17/249,640 external-priority patent/US11214811B1/en
Priority claimed from US17/302,121 external-priority patent/US11242534B1/en
Priority claimed from US17/302,110 external-priority patent/US20220030822A1/en
Priority claimed from US17/302,739 external-priority patent/US11326177B2/en
Priority claimed from US17/303,116 external-priority patent/US11369073B2/en
Application filed by Inari Agriculture Technology Inc filed Critical Inari Agriculture Technology Inc
Priority claimed from PCT/US2021/043207 external-priority patent/WO2022026403A2/en
Publication of EP4172342A2 publication Critical patent/EP4172342A2/de
Publication of EP4172342A4 publication Critical patent/EP4172342A4/de
Pending legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213Targeted insertion of genes into the plant genome by homologous recombination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/20Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP21849192.6A 2020-07-31 2021-07-26 Entfernbare transgene pflanzenloci mit verwandten rna-führungserkennungsstellen Pending EP4172342A4 (de)

Applications Claiming Priority (18)

Application Number Priority Date Filing Date Title
US202063059916P 2020-07-31 2020-07-31
US202063059963P 2020-07-31 2020-07-31
US202063059860P 2020-07-31 2020-07-31
US202063059813P 2020-07-31 2020-07-31
US202163199930P 2021-02-03 2021-02-03
US202163199951P 2021-02-04 2021-02-04
US202163199949P 2021-02-04 2021-02-04
US17/248,936 US11359210B2 (en) 2020-07-31 2021-02-12 INIR12 transgenic maize
US17/249,640 US11214811B1 (en) 2020-07-31 2021-03-08 INIR6 transgenic maize
US202163201030P 2021-04-09 2021-04-09
US202163201029P 2021-04-09 2021-04-09
US17/302,121 US11242534B1 (en) 2020-07-31 2021-04-23 INHT31 transgenic soybean
US17/302,110 US20220030822A1 (en) 2020-07-31 2021-04-23 Inht26 transgenic soybean
US17/302,739 US11326177B2 (en) 2020-07-31 2021-05-11 INIR12 transgenic maize
US17/303,116 US11369073B2 (en) 2020-07-31 2021-05-20 INIR12 transgenic maize
US202163202569P 2021-06-16 2021-06-16
US202163203137P 2021-07-09 2021-07-09
PCT/US2021/043207 WO2022026403A2 (en) 2020-07-31 2021-07-26 Removable plant transgenic loci with cognate guide rna recognition sites

Publications (2)

Publication Number Publication Date
EP4172342A2 EP4172342A2 (de) 2023-05-03
EP4172342A4 true EP4172342A4 (de) 2024-10-02

Family

ID=85128636

Family Applications (1)

Application Number Title Priority Date Filing Date
EP21849192.6A Pending EP4172342A4 (de) 2020-07-31 2021-07-26 Entfernbare transgene pflanzenloci mit verwandten rna-führungserkennungsstellen

Country Status (3)

Country Link
EP (1) EP4172342A4 (de)
BR (1) BR112023001774A2 (de)
CA (1) CA3188406A1 (de)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001066780A2 (en) * 2000-03-09 2001-09-13 Dekalb Genetics Corporation Homologous recombination-mediated transgene alterations in plants
WO2002012517A1 (en) * 2000-08-04 2002-02-14 University Of Victoria Innovation And Development Corporation Plant promoter derived from luminal binding protein gene and methods for its use
WO2006003186A1 (en) * 2004-07-06 2006-01-12 Biogemma Method for enhancing gene expression in plants
WO2013116700A1 (en) * 2012-02-01 2013-08-08 Dow Agrosciences Llc Glyphosate resistant plants and associated methods
WO2015118640A1 (ja) * 2014-02-06 2015-08-13 日本たばこ産業株式会社 植物の形質転換細胞の取得方法
WO2019018534A1 (en) * 2017-07-18 2019-01-24 The Board Of Trustees Of Leland Stanford Junior University GENOME EDITION WITHOUT SCARCE THROUGH TWO-STEP APPROVED REPAIR BY HOMOLOGY

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001066780A2 (en) * 2000-03-09 2001-09-13 Dekalb Genetics Corporation Homologous recombination-mediated transgene alterations in plants
WO2002012517A1 (en) * 2000-08-04 2002-02-14 University Of Victoria Innovation And Development Corporation Plant promoter derived from luminal binding protein gene and methods for its use
WO2006003186A1 (en) * 2004-07-06 2006-01-12 Biogemma Method for enhancing gene expression in plants
WO2013116700A1 (en) * 2012-02-01 2013-08-08 Dow Agrosciences Llc Glyphosate resistant plants and associated methods
WO2015118640A1 (ja) * 2014-02-06 2015-08-13 日本たばこ産業株式会社 植物の形質転換細胞の取得方法
WO2019018534A1 (en) * 2017-07-18 2019-01-24 The Board Of Trustees Of Leland Stanford Junior University GENOME EDITION WITHOUT SCARCE THROUGH TWO-STEP APPROVED REPAIR BY HOMOLOGY

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ALI ZAHIR ET AL: "Fusion of the Cas9 endonuclease and the VirD2 relaxase facilitates homology-directed repair for precise genome engineering in rice", COMMUNICATIONS BIOLOGY, vol. 3, no. 1, 23 January 2020 (2020-01-23), pages 1 - 13, XP055910384, Retrieved from the Internet <URL:http://www.nature.com/articles/s42003-020-0768-9> DOI: 10.1038/s42003-020-0768-9 *
CAI ZHENKUN ET AL: "Broadening the targetable space: engineering and discovery of PAM-flexible Cas proteins", TRENDS IN MICROBIOLOGY, 1 May 2024 (2024-05-01), GB, XP093192173, ISSN: 0966-842X, DOI: 10.1016/j.tim.2024.05.004 *
CHRISTIAN MICHELLE ET AL: "Targeted mutagenesis of Arabidopsis thaliana using engineered TAL effector nucleases", G3 (BETHESDA, MD.), 3 October 2013 (2013-10-03), United States, pages 1697 - 1705, XP055902232, Retrieved from the Internet <URL:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3789794/pdf/1697.pdf> [retrieved on 20220316], DOI: 10.1534/g3.113.007104 *
SRIVASTAVA VIBHA ET AL: "Dual-targeting by CRISPR/Cas9 for precise excision of transgenes from rice genome", PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC), SPRINGER NETHERLANDS, DORDRECHT, vol. 129, no. 1, 20 January 2017 (2017-01-20), pages 153 - 160, XP036194323, ISSN: 0167-6857, [retrieved on 20170120], DOI: 10.1007/S11240-016-1166-3 *
WANG JUNJIE ET AL: "Generation of marker-free transgenic rice using CRISPR/Cas9 system controlled by floral specific promoters", JOURNAL OF GENETICS AND GENOMICS, vol. 46, no. 1, 2019, pages 61 - 64, XP085616545, ISSN: 1673-8527, DOI: 10.1016/J.JGG.2018.11.005 *

Also Published As

Publication number Publication date
EP4172342A2 (de) 2023-05-03
CA3188406A1 (en) 2022-02-03
BR112023001774A2 (pt) 2023-03-07

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