EP4182425A1 - Procédé de fermentation et bioréacteur pour la mise en oeuvre de procédés de fermentation anaérobie, aérobie et micro-air, comprenant un système d'aération de type déversoir - Google Patents

Procédé de fermentation et bioréacteur pour la mise en oeuvre de procédés de fermentation anaérobie, aérobie et micro-air, comprenant un système d'aération de type déversoir

Info

Publication number
EP4182425A1
EP4182425A1 EP21734788.9A EP21734788A EP4182425A1 EP 4182425 A1 EP4182425 A1 EP 4182425A1 EP 21734788 A EP21734788 A EP 21734788A EP 4182425 A1 EP4182425 A1 EP 4182425A1
Authority
EP
European Patent Office
Prior art keywords
gas
bioreactor
gassing
space
fermentation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP21734788.9A
Other languages
German (de)
English (en)
Inventor
Jan-henryk RICHTER-LISTEWNIK
Reinhard PÄTZ
Sören THIERING
Joachim Schütze
Leonhard Jagusch
Werner Schönherr
Felix Langer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Anhalt University of Applied Sciences
Original Assignee
Anhalt University of Applied Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Anhalt University of Applied Sciences filed Critical Anhalt University of Applied Sciences
Publication of EP4182425A1 publication Critical patent/EP4182425A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C12M29/08—Air lift
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/20—Mixing gases with liquids
    • B01F23/23—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids
    • B01F23/232—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids using flow-mixing means for introducing the gases, e.g. baffles
    • B01F23/2323—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids using flow-mixing means for introducing the gases, e.g. baffles by circulating the flow in guiding constructions or conduits
    • B01F23/23231—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids using flow-mixing means for introducing the gases, e.g. baffles by circulating the flow in guiding constructions or conduits being at least partially immersed in the liquid, e.g. in a closed circuit
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F25/50—Circulation mixers, e.g. wherein at least part of the mixture is discharged from and reintroduced into a receptacle
    • B01F25/54—Circulation mixers, e.g. wherein at least part of the mixture is discharged from and reintroduced into a receptacle provided with a pump inside the receptacle to recirculate the material within the receptacle
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/02—Aerobic processes
    • C02F3/12—Activated sludge processes
    • C02F3/22—Activated sludge processes using circulation pipes
    • C02F3/223—Activated sludge processes using circulation pipes using "air-lift"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
    • C02F2203/006—Apparatus and plants for the biological treatment of water, waste water or sewage details of construction, e.g. specially adapted seals, modules, connections
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/02—Temperature
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/04—Oxidation reduction potential [ORP]
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/06—Controlling or monitoring parameters in water treatment pH
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/10—Biological treatment of water, waste water, or sewage

Definitions

  • Fermentation process and bioreactor for carrying out anaerobic, aerobic and micro-aerophilic fermentations including an airlift shaft overflow
  • DE 41 12 377 describes a compact reactor consisting of a biologically active reaction chamber with a device for gassing the waste water.
  • the gassing takes place by means of a circulating gassing system, which comprises an upflow tube, a pump, a deflection hood and a downflow tube.
  • DE 41 12 378 also describes a compact reactor consisting of a biologically active reaction chamber with a device for gassing the waste water.
  • the reactors described in the two publications mentioned above are adapted to processes for aerobic biological wastewater treatment.
  • devices for wastewater treatment are open systems that allow air to be exchanged with the environment.
  • a precise control of the supply of gaseous substrates, as is required in fermentation processes, and internal circulation to the goal of optimal gas utilization does not take place.
  • this embodiment is not suitable for enabling optimal aeration in terms of energy under changing operating conditions, as is the case with aerobic batch fermentations or microaerophilic gassing.
  • the object of the invention is now to provide an efficient fermentation process and an efficient bioreactor for carrying out such a process.
  • the problems of the methods currently known from the prior art are to be overcome.
  • a fermentation method and a bioreactor for carrying out such a method are to be provided, which are efficient with regard to the energy requirement and variable with regard to the process control.
  • the invention therefore relates to a fermentation process which is carried out using a bioreactor, the bioreactor having: a reaction space with a gas space and a liquid space; a fresh gas inlet and a gas outlet, wherein the gas inlet is designed to introduce gases to the gassing device and the gas outlet is designed to conduct gaseous products out of the gas space; in a vertical circulation gassing system comprising an upflow pipe, a pump, a gassing device and a downflow pipe, which is arranged around the upflow pipe and the gassing device, wherein the vertical circulation gassing system is designed to introduce gas from the gas space into the liquid space; the method comprising the steps of:
  • Fresh gas is sucked in automatically via the fresh gas inlet or fresh gas inlet, preferably by the gassing device, known as the airlift shaft overflow, and the exhaust gas including gaseous products is discharged from the bioreactor via a gas outlet.
  • the gassing device known as the airlift shaft overflow
  • the airlift shaft overflow can always be operated in the optimum efficiency range of the circulating pump in all operating states by changing the required delivery height and changing the parameters of the circulating pump by changing the speed.
  • the fermentation process according to the invention and the bioreactor are efficient with regard to the energy requirement and variable with regard to the process control.
  • An essential advantage of the method according to the invention is that the gas input and the gas conversion in the course of the fermentation process can be energetically optimally adapted to the respectively changed process conditions. In particular, no gas compression is required for use in the bioreactor.
  • the gassing device of the present invention works preferably according to the immersion jet gassing principle and is more preferably based on the known Submerged jet hydro-aeration (TSH aeration) optimized and further developed for use in biotechnology.
  • TSH aeration Submerged jet hydro-aeration
  • an inner, downward gassed circulation flow is preferably generated, in which the gassed circulation flow is guided downwards to the outlet of the outflow pipe and then rises and degasses in the bioreactor with turbulence dissolution and back-mixing due to its lower density.
  • the reactor according to the invention is preferably an airlift loop reactor with a downward gassed circulation flow, suitable for fermentation processes with changing specific gas requirements and for the use of gaseous substrates (3rd generation biotechnology) in the fermentation process.
  • the gassing device of the circulating gassing system is preferably designed as a height-adjustable deflection hood in order to ensure an energetically optimal circulation and gassing with variable filling levels in the bioreactor.
  • the deflection hood has gas inlet channels at the upper end.
  • a rapidly sedimenting yeast is used.
  • the fermentation time is shortened thanks to optimal process conditions and the emptying time is shortened thanks to the use of rapidly sedimenting yeast.
  • the fermentation process is controlled by measuring the dissolved oxygen concentration, substrate concentration and ethanol concentration in the bioreactor 11 and the measured CO2 concentration in the exhaust air.
  • the fermentation process can be remotely controlled using modern communication systems such as smartphones.
  • Step 3 tact sedimentation of the biomass
  • the clear runoff harvested from the bioreactor 11 is first collected in the storage tank 80 .
  • the liquid medium is processed as a bioreactor outflow by means of distillation and/or rectification 82.
  • the outflow is withdrawn via an outflow register
  • Bioreactor effluent contains only 5 to 20% of the original solids in the bioreactor.
  • This solid-reduced effluent from the fermentation is fed to the ethanol distillation and/or rectification 82 in order to produce an ethanol 83 there with a purity of preferably 96% by volume.
  • the stillage 96 that has been drawn off is preferably passed through a clarification separator 90 .
  • the yeast cells and/or solids that are still present are separated and concentrated in the sludge collection chamber of the separator.
  • the concentrated biomass suspension 92 preferably of about 20 to 24% TS, is periodically discharged via the internal control of the separator. These separated and concentrated yeast cells have already been partially thermolysed in the distillation.
  • this biomass suspension 92 is subjected to a final thermolysis or an enzymatic digestion 94 .
  • the use of a concentrated biomass suspension reduces the technical effort for the thermolysis and/or the enzymatic digestion.
  • Part of the clear runoff 99 from the separation can also be reused to dilute the molasses 26 in order to adjust the substrate concentration when the fermentation substrate solution is prepared. It is thus possible for up to 40% of the bioreactor effluent from each fermentation batch to be returned to the process. The need for process water 22 can be significantly reduced by this reuse of the resulting stillage 96 . The waste water load or the amount of stillage to be disposed of is reduced in the same ratio. In a further step, the biomass suspension and excess suspension 72 are processed and the residue 70 of the bioreactor 11 is emptied.
  • the reaction container 11 is cleaned by means of cleaning in place (CIP) 74 only after the end of a batch series and thus after the reactor container has been emptied of residues.
  • CIP cleaning in place
  • the rinsing water tank (First Rinse) is then refilled with clear water (Last Rinse) and the clear water tank with the corresponding amount of new fresh process water.
  • the losses in the CIP solution (NaOH solution) are filled using the well-known method of "sharpening" (filling up with a new NaOH solution according to the pH value).

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Sustainable Development (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé et un bioréacteur pour la mise en œuvre du procédé, comprenant un dispositif d'aspersion se présentant sous la forme d'un système d'aération de type déversoir. Le bioréacteur est approprié pour des procédés de fermentation anaérobie, aérobie et micro-air. Le dispositif d'aération est de préférence auto-amorçage et permet le recyclage de gaz par recirculation, l'alimentation en gaz requise étant découplée de l'intensité de barbotage et de l'apport énergétique, et le bioréacteur est donc approprié pour être utilisé avec des substrats gazeux.
EP21734788.9A 2020-07-15 2021-06-17 Procédé de fermentation et bioréacteur pour la mise en oeuvre de procédés de fermentation anaérobie, aérobie et micro-air, comprenant un système d'aération de type déversoir Withdrawn EP4182425A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102020118668.1A DE102020118668A1 (de) 2020-07-15 2020-07-15 Fermentationsverfahren und Bioreaktor zur Durchführung von anaeroben, aeroben und mikro-aerophilen Fermentationen umfassend einen Airlift-Schachtüberfall
PCT/EP2021/066340 WO2022012843A1 (fr) 2020-07-15 2021-06-17 Procédé de fermentation et bioréacteur pour la mise en oeuvre de procédés de fermentation anaérobie, aérobie et micro-air, comprenant un système d'aération de type déversoir

Publications (1)

Publication Number Publication Date
EP4182425A1 true EP4182425A1 (fr) 2023-05-24

Family

ID=76624025

Family Applications (1)

Application Number Title Priority Date Filing Date
EP21734788.9A Withdrawn EP4182425A1 (fr) 2020-07-15 2021-06-17 Procédé de fermentation et bioréacteur pour la mise en oeuvre de procédés de fermentation anaérobie, aérobie et micro-air, comprenant un système d'aération de type déversoir

Country Status (3)

Country Link
EP (1) EP4182425A1 (fr)
DE (1) DE102020118668A1 (fr)
WO (1) WO2022012843A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12441974B2 (en) 2020-02-05 2025-10-14 Mott Corporation Bioreactor design and methods of manufacture thereof
EP4324905A1 (fr) * 2022-08-19 2024-02-21 Sartorius Stedim Biotech GmbH Système de bioréacteur avec installation de fumigation

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3331993A1 (de) * 1983-09-05 1984-03-29 Heinz Prof. Dr.-Ing. 7261 Gechingen Blenke Verfahren und vorrichtung zur entgasung von gas-liquid-systemen ohne schaumbildung
DE4112378A1 (de) 1991-04-16 1992-10-22 Leonhard Jagusch Vorrichtung zur begasung von fluessigkeiten
DE4112377C2 (de) 1991-04-16 2002-10-24 Leonhard Jagusch Kompaktreaktor für die aerobe biologische Abwasserreinigung
DE4316376A1 (de) * 1993-05-15 1994-12-01 Onken Ulfert Prof Dr Verfahren zur kontinuierlichen Kultivierung von wandlosen Suspensionszellen
DE102005026027A1 (de) 2004-06-03 2006-02-23 Christian Widmer Stofflöser, Reaktor für Hydrolyse und/oder Nassrotte und Abfallaufbereitungsanlage mit einem derartigen Stofflöser und Reaktor
IES20070543A2 (en) * 2006-08-01 2008-07-23 Celljet Biotech Ltd An airlift bioreactor
DE102008029307A1 (de) * 2008-06-20 2009-12-24 Bayer Technology Services Gmbh Verfahren und Vorrichtung zur Rückhaltung und Rückführung von Zellen
US11434461B2 (en) 2018-03-20 2022-09-06 Keck Graduate Institute Of Applied Life Sciences Airlift perfusion bioreactor for the culture of cells

Also Published As

Publication number Publication date
WO2022012843A1 (fr) 2022-01-20
DE102020118668A1 (de) 2022-01-20

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