EP4326864A4 - Nouvelles nucléases crispr omni 117, 140, 150-158, 160-165, 167-177, 180-188, 191-198, 200, 201, 203, 205-209, 211-217, 219, 220, 222, 223, 226, 227, 229, 231-236, 238-245, 247, 250, 254, 256, 257, 260 et 262 - Google Patents

Nouvelles nucléases crispr omni 117, 140, 150-158, 160-165, 167-177, 180-188, 191-198, 200, 201, 203, 205-209, 211-217, 219, 220, 222, 223, 226, 227, 229, 231-236, 238-245, 247, 250, 254, 256, 257, 260 et 262

Info

Publication number
EP4326864A4
EP4326864A4 EP22792515.3A EP22792515A EP4326864A4 EP 4326864 A4 EP4326864 A4 EP 4326864A4 EP 22792515 A EP22792515 A EP 22792515A EP 4326864 A4 EP4326864 A4 EP 4326864A4
Authority
EP
European Patent Office
Prior art keywords
nucleases
omni
new crispr
crispr
new
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP22792515.3A
Other languages
German (de)
English (en)
Other versions
EP4326864A1 (fr
Inventor
Lior IZHAR
Nadav MARBACH BAR
Liat ROCKAH
Nurit MERON
Ophir ADIV TAL
Ariel GISPAN
Idit BUCH
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Emendobio Inc
Original Assignee
Emendobio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Emendobio Inc filed Critical Emendobio Inc
Publication of EP4326864A1 publication Critical patent/EP4326864A1/fr
Publication of EP4326864A4 publication Critical patent/EP4326864A4/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Peptides Or Proteins (AREA)
EP22792515.3A 2021-04-22 2022-04-21 Nouvelles nucléases crispr omni 117, 140, 150-158, 160-165, 167-177, 180-188, 191-198, 200, 201, 203, 205-209, 211-217, 219, 220, 222, 223, 226, 227, 229, 231-236, 238-245, 247, 250, 254, 256, 257, 260 et 262 Pending EP4326864A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163178364P 2021-04-22 2021-04-22
US202163211123P 2021-06-16 2021-06-16
PCT/US2022/025803 WO2022226215A1 (fr) 2021-04-22 2022-04-21 Nouvelles nucléases crispr omni 117, 140, 150-158, 160-165, 167-177, 180-188, 191-198, 200, 201, 203, 205-209, 211-217, 219, 220, 222, 223, 226, 227, 229, 231-236, 238-245, 247, 250, 254, 256, 257, 260 et 262

Publications (2)

Publication Number Publication Date
EP4326864A1 EP4326864A1 (fr) 2024-02-28
EP4326864A4 true EP4326864A4 (fr) 2025-06-18

Family

ID=83723152

Family Applications (1)

Application Number Title Priority Date Filing Date
EP22792515.3A Pending EP4326864A4 (fr) 2021-04-22 2022-04-21 Nouvelles nucléases crispr omni 117, 140, 150-158, 160-165, 167-177, 180-188, 191-198, 200, 201, 203, 205-209, 211-217, 219, 220, 222, 223, 226, 227, 229, 231-236, 238-245, 247, 250, 254, 256, 257, 260 et 262

Country Status (8)

Country Link
US (1) US20250011740A1 (fr)
EP (1) EP4326864A4 (fr)
JP (1) JP2024516161A (fr)
KR (1) KR20240052720A (fr)
AU (1) AU2022262623A1 (fr)
CA (1) CA3216102A1 (fr)
IL (1) IL307855A (fr)
WO (1) WO2022226215A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2024506608A (ja) 2021-02-08 2024-02-14 エメンドバイオ・インコーポレイテッド Ｏｍｎｉ－１０３ ｃｒｉｓｐｒヌクレアーゼ
US12091688B2 (en) 2021-02-08 2024-09-17 Emendobio Inc. OMNI-103 CRISPR nuclease
EP4705347A2 (fr) * 2023-04-28 2026-03-11 Apogee Therapeutics, Inc. Anticorps qui se lient à ox40l et procédés d'utilisation

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018035388A1 (fr) * 2016-08-17 2018-02-22 The Broad Institute, Inc. Systèmes et nouvelles enzymes crispr et systèmes
US20190264232A1 (en) * 2018-02-23 2019-08-29 Pioneer Hi-Bred International, Inc. Novel cas9 orthologs
WO2019222555A1 (fr) * 2018-05-16 2019-11-21 Arbor Biotechnologies, Inc. Nouveaux systèmes et composants associés à crispr
WO2020069029A1 (fr) * 2018-09-26 2020-04-02 Emendobio Inc. Nouvelles nucléases crispr
WO2020223514A2 (fr) * 2019-04-30 2020-11-05 Emendobio Inc. Nouvelle nucléase crispr omni-50
WO2021030344A1 (fr) * 2019-08-12 2021-02-18 Lifeedit, Inc. Nucléases guidées par arn et fragments actifs et variants associés et méthodes d'utilisation

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6799586B2 (ja) * 2015-08-28 2020-12-16 ザ ジェネラル ホスピタル コーポレイション 遺伝子操作ＣＲＩＳＰＲ−Ｃａｓ９ヌクレアーゼ
US20220213456A1 (en) * 2019-04-30 2022-07-07 Emendobio Inc. Novel omni crispr nucleases

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018035388A1 (fr) * 2016-08-17 2018-02-22 The Broad Institute, Inc. Systèmes et nouvelles enzymes crispr et systèmes
US20190264232A1 (en) * 2018-02-23 2019-08-29 Pioneer Hi-Bred International, Inc. Novel cas9 orthologs
WO2019222555A1 (fr) * 2018-05-16 2019-11-21 Arbor Biotechnologies, Inc. Nouveaux systèmes et composants associés à crispr
WO2020069029A1 (fr) * 2018-09-26 2020-04-02 Emendobio Inc. Nouvelles nucléases crispr
WO2020223514A2 (fr) * 2019-04-30 2020-11-05 Emendobio Inc. Nouvelle nucléase crispr omni-50
WO2021030344A1 (fr) * 2019-08-12 2021-02-18 Lifeedit, Inc. Nucléases guidées par arn et fragments actifs et variants associés et méthodes d'utilisation

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ALKHNBASHI OMER S ET AL: "CRISPR-Cas bioinformatics", METHODS, ACADEMIC PRESS, NL, vol. 172, 19 July 2019 (2019-07-19), pages 3 - 11, XP086083207, ISSN: 1046-2023, [retrieved on 20190719], DOI: 10.1016/J.YMETH.2019.07.013 *
CUI YINGBO ET AL: "Review of CRISPR/Cas9 sgRNA Design Tools", INTERDISCIPLINARY SCIENCES: COMPUTATIONAL LIFE SCIENCES, INTERNATIONAL ASSOCIATION OF SCIENTISTS IN THE INTERDISCIPLINARY AREAS, CA, vol. 10, no. 2, 11 April 2018 (2018-04-11), pages 455 - 465, XP036502047, ISSN: 1913-2751, [retrieved on 20180411], DOI: 10.1007/S12539-018-0298-Z *
MICHAEL K JENSEN: "Design principles for nuclease-deficient CRISPR-based transcriptional regulators", FEMS YEAST RESEARCH, vol. 18, no. 4, 10 April 2018 (2018-04-10), XP055520704, DOI: 10.1093/femsyr/foy039 *
SANJARBEK HUDAIBERDIEV ET AL: "Phylogenomics of Cas4 family nucleases", BMC EVOLUTIONARY BIOLOGY, BIOMED CENTRAL LTD, LONDON, UK, vol. 17, no. 1, 28 November 2017 (2017-11-28), pages 1 - 14, XP021250909, DOI: 10.1186/S12862-017-1081-1 *
See also references of WO2022226215A1 *

Also Published As

Publication number Publication date
AU2022262623A1 (en) 2023-11-30
AU2022262623A9 (en) 2023-12-07
IL307855A (en) 2023-12-01
JP2024516161A (ja) 2024-04-12
KR20240052720A (ko) 2024-04-23
EP4326864A1 (fr) 2024-02-28
WO2022226215A8 (fr) 2023-06-15
WO2022226215A1 (fr) 2022-10-27
US20250011740A1 (en) 2025-01-09
CA3216102A1 (fr) 2022-10-27

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