EP4622460A1 - Procédés d'amélioration des caractéristiques d'une plante - Google Patents

Procédés d'amélioration des caractéristiques d'une plante

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Publication number
EP4622460A1
EP4622460A1 EP23809590.5A EP23809590A EP4622460A1 EP 4622460 A1 EP4622460 A1 EP 4622460A1 EP 23809590 A EP23809590 A EP 23809590A EP 4622460 A1 EP4622460 A1 EP 4622460A1
Authority
EP
European Patent Office
Prior art keywords
plant
cyclobutrifluram
composition
soil
medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP23809590.5A
Other languages
German (de)
English (en)
Inventor
Claudio Screpanti
Ben OYSERMAN
Alessandro BERGNA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Syngenta Crop Protection AG Switzerland
Original Assignee
Syngenta Crop Protection AG Switzerland
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Crop Protection AG Switzerland filed Critical Syngenta Crop Protection AG Switzerland
Publication of EP4622460A1 publication Critical patent/EP4622460A1/fr
Pending legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/06—Coating or dressing seed
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G29/00—Root feeders; Injecting fertilisers into the roots
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P13/00—Herbicides; Algicides
    • A01P13/02—Herbicides; Algicides selective
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00—Fungicides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P5/00—Nematocides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/41—Rhizobium

Definitions

  • the present invention relates to the use of 4-membered ring carboxamide compounds, in particular cyclobutylcarboxamides, for the stimulation of vesicular-arbuscular mycorrhizal fungi.
  • the present invention relates to methods and compositions which use the cyclobutylcarboxamides to stimulate the growth of plant materials in the presence of the vesicular arbuscular mycorrhizal fungi, at the same time as enhancing pest control, and/or facilitate soil bioremediation.
  • the present invention also relates to methods for agricultural use of these compositions, which alter the arbuscular mycorrhizal fungi population in a manner that is beneficial to a plant, by promoting favourable arbuscular mycorrhizal fungal activity.
  • vesicular arbuscular mycorrhizae or “arbuscular mycorrhizal fungi” and are known to occur in at least 300,000 plant species, including most agriculturally important crops, with the exception of crucifers and a few other species.
  • the vesicular arbuscular mycorrhizae fungi can have profound beneficial effects on plant growth, nutrition and tolerance to both abiotic and biotic stress.
  • fungi While present in principle in any terrestrial habitat, such fungi may also be actively used as plant or soil inoculants, and thereby may reduce fertilizer use, reduce crop loss due to abiotic and biotic stresses. In addition, these fungi have been shown to improve the survival and early growth plantings and increase productivity or revegetation in poor and disturbed sites. Among the biotic stress factors affecting plants, nematode and fungi pathogens are causing increasing concern.
  • pathogens have been usually combated by the use of nematicidal and/or fungicidal agrochemicals.
  • such compounds tend to negatively affect the soil microbiome, in particular the growth of the fungi involved in the formation of vesicular arbuscular mycorrhizae as an off-target effect.
  • fungi and nematode pathogens rapidly have developed resistance to many of the agrochemical compounds employed, resulting in a reduced efficacy over time. Accordingly, there remain a need to provide for agrochemicals that can provide nematocidal aswell as fungicidal control, while not affecting or even enhancing the growth of arbuscular mycorrhizal fungi.
  • the present invention relates to a method of enhancing a plant characteristic of a plant growing in a medium in the presence of a vesicular-arbuscular mycorrhizae fungal culture, comprising applying an effective amount of a composition comprising cyclobutrifluram to the plant, the locus or the medium, or plant propagation material.
  • the plant characteristic comprises plant stand, plant root growth, and/or plant vigour.
  • the present invention also relates to the use of a composition comprising cyclobutrifluram for growth of vesicular-arbuscular mycorrhizal fungi in a medium comprising a plant, the locus, or propagation material thereof, as well as maintaining or improving the overall soil microbiome health, preserving soil biodiversity.
  • the present invention relates to a method according to the invention, wherein cyclobutrifluram is applied onto a seed or propagation material in an amount of from between 1 gram and up to and including 1000 gram cyclobutrifluram per 100 kg seeds or propagation material, preferably, wherein cyclobutrifluram is applied onto the seed or propagation material in an amount of between 10 grams and 400 grams cyclobutrifluram per 100 kg seeds or propagation material; and yet more preferably wherein cyclobutrifluram is applied onto the seed or propagation material in an amount of between 20 grams and 100 grams cyclobutrifluram per 100 kg seeds or propagation material.
  • the compounds of form ula (I) according to the invention are preventively and/or curatively valuable active ingredients in the field of pest control, even at low rates of application, which can be used against pesticide resistant pests such as insects and fungi, which compounds of form ula (I) have a very favourable biocidal spectrum and are well tolerated by warm -blooded species, fish and plants.
  • the compounds of form ula I according to the invention have, for practical purposes, a very advantageous spectrum of activities for protecting pants and soil m icrobiome health, and are therefore particularly useful for protecting plants against attack and damage by nematodes, and for increasing plant and soil health.
  • the present invention makes use of a composition comprising compounds of the invention, according to form ula (I), having received the ISO name cyclobutrifluram :
  • Cyclobutrifluram generally comprises one or more of the cis-conformed enantiomers of N-2-(2,4-dichlorophenyl)cyclobutyl]-2-(trifluoro- methyl)pyridine-3-carboxam ide.
  • the racemic compound of cyclobutrifluram is a 1 :1 mixture of the compounds of formula (la) and (lb).
  • the wedged bonds shown in the compounds of formula (la) and (lb) represent absolute stereochemistry, whereas the thick straight bonds such as those shown for compound of formula (I) represent relative stereochemistry in racemic compounds. It has also surprisingly been found that one enantiomer of cyclobutrifluram is particularly useful in a method for controlling or preventing infestation of plants by a phytopathogenic microorganism of the genus Aspergillus.
  • cyclobutrifluram is generally applied as part of a pesticidal composition, whereby it is known that the compounds of formula (I) are especially useful for the control of nematodes.
  • a method for controlling or preventing infestation of plants by a nematode plant pest comprising applying to a crop of plants, the locus thereof, or propagation material thereof a pesticidal composition comprising cyclobutrifluram and one or more formulation adjuvants, simultaneously with improving the soil microbiome according to the invention.
  • the ratio of the (1 S.2S) stereoisomer to its enantiomer (1 R,2R) is preferably greater than 1 :1 . More preferably, the ratio of the (1 S,2S) to (1 S,2S) is greater than 1 .5:1 , more preferably greater than 2.5:1 , especially greater than 4:1 , advantageously greater than 9:1 , desirably greater than 20:1 , in particular greater than 35:1 .
  • the ratio of the cis isomer to its trans isomer is greater than 1 .5:1 , more preferably greater than 2.5:1 , especially greater than 4:1 , advantageously greater than 9:1 , desirably greater than 20:1 , in particular greater than 35:1 .
  • composition is a suspension concentrate composition.
  • composition is a suspension concentrate composition.
  • method for the use of the composition comprising the steps of: providing cyclobutrifluram or a composition comprising cyclobutrifluram as defined in any one of embodiments herein above; and applying the composition to a propagation material; and planting of the propagation material into a medium, preferably a soil portion comprising arbuscular mycorrhizal fungi.
  • a method for the use of the composition comprising the steps of: providing cyclobutrifluram or a composition comprising cyclobutrifluram as defined in any one of embodiments herein above; and applying the composition to a plant, propagation material, or the locus thereof.
  • a method for growing a plant comprising applying or treating a propagation material thereof with cyclobutrifluram or a I composition comprising cyclobutrifluram as defined herein above, for the simultaneous or subsequent enhancement of beneficial arbuscular mycorrhizal fungi activity int the surrounding soil.
  • the propagation material is a seed. More preferably, cyclobutrifluram is applied onto the seed in an amount of between 1 grams and 1000 grams cyclobutrifluram per 100 kg seeds. Yet more preferably, cyclobutrifluram is applied onto the seed in an amount of between 10 grams and 400 grams of cyclobutrifluram per 100 kg seeds, yet again more preferably wherein cyclobutrifluram is applied onto the seed in an amount of between 20 grams and 100 grams pf cyclobutrifluram per 100 kg seeds.
  • the method provided herein may be applied to useful plants.
  • the method provided herein may be applied to useful plants.
  • the application of the compounds of the present invention to seeds is a preferred application method.
  • the compounds of the invention and compositions thereof are also suitable for the protection of plant propagation material, for example seeds, such as fruit, tubers or kernels, or nursery plants, against pests of the abovementioned type.
  • the propagation material can be treated with the compound prior to planting, for example seed can be treated prior to sowing.
  • the compound can be applied to seed kernels (coating), either by soaking the kernels in a liquid composition or by applying a layer of a solid composition. It is also possible to apply the compositions when the propagation material is planted to the site of application, for example into the seed furrow during drilling.
  • the method according to the invention can advantageously be also used for controlling or destroying pests such as insects and/or fungi which occur in particular on plants, especially on useful plants and ornamentals in agriculture, in horticulture and in forests, or on organs, such as fruits, flowers, foliage, stalks, tubers, seeds or roots, of such plants, and in some cases even plant organs which are formed at a later point in time remain protected against these pests.
  • pests such as insects and/or fungi which occur in particular on plants, especially on useful plants and ornamentals in agriculture, in horticulture and in forests, or on organs, such as fruits, flowers, foliage, stalks, tubers, seeds or roots, of such plants, and in some cases even plant organs which are formed at a later point in time remain protected against these pests.
  • the compounds of formula I according to the invention have, for practical purposes, a very advantageous spectrum of activities for protecting pants and soil microbiome health, and are therefore particularly useful for protecting plants against attack and damage by nematodes, and for increasing plant soil health, and for increasing the growth of symbiotic arbuscular mycorrhizal fungi.
  • the root development of plants growing in the medium is increased compared to the roots of plants growing in untreated medium.
  • the methods may further comprise treating a seed, seedling root or plant, or combinations thereof, with an inoculant composition.
  • agronomically beneficial describes compositions that, when applied to soil, seeds, seedlings, roots or plants, result in enhancement (which may be statistically significant) of plant characteristics such as plant stand, root growth, and/or vigour in comparison to non-treated soil, seeds, seedlings, roots or plants.
  • enhancing or “enhancement,” when used herein, refers to improvement (which may be statistically significant) in any of the above plant characteristics in comparison to non-treated soil, seeds, seedlings, roots or plants.
  • the method preferably further comprises controlling a plant pest present in the medium in an amount sufficient to harm or reduce the growth of a plant growing in the medium.
  • the plant pest comprises one or more endoparasitic, semi-endoparasitic and ectoparasitic nematodes; and/or one or more harmful bacteria or fungi.
  • the composition comprising cyclobutrifluram is applied via irrigation, soil drenching, infurrow application, and/or as a seed pre-treatment or inoculation.
  • the method preferably further comprises applying the composition comprising cyclobutrifluram is conducted prior to planting, or after planting.
  • the method preferably further comprises adding a vesicular-arbuscular mycorrhizae fungal culture to the medium prior to, or during the planting.
  • composition comprising cyclobutrifluram when applied onto a seed or propagation material, an amount of between 1 grams and 120 grams cyclobutrifluram per 100 kg seeds or propagation material is advantageously applied.
  • the composition comprising cyclobutrifluram further comprises one or more additional biologically active agents, preferably one or more acaricide, bactericide, fungicide, insecticide, nematocide, and/or plant activator.
  • additional biologically active agents preferably one or more acaricide, bactericide, fungicide, insecticide, nematocide, and/or plant activator.
  • the method preferably further comprises prising treating the seeds, seedlings, roots or plants, or combinations thereof, with an inoculant composition.
  • the inoculant composition comprises a rhizobial species and/or a vesicular-arbuscular mycorrhizae fungal culture. More preferably, the inoculant composition comprises Bradyrhizobium japonicum, Bradyrhizobium parasponia, Snorhizobium meliloti, Rhizobium leguminosarum biovars phaseoli, viceae, and trifolii, Rhizobium lupini, or Bradyrhizobium sp. (Arachis), or combinations thereof.
  • the plant is a leguminous plant.
  • the leguminous plant comprises soybean, alfalfa, peanut, pea, lentil, bean, or clover, and preferably wherein the leguminous seeds are inoculated with a Bradyrhizobium spp., more preferably Bradyrhizobium japonicum.
  • the medium is at least in part a solid soil comprising soil, peat, sand, clay, compost, Cocos granulate or fibre, sawdust, paper pulp, crop stalks or straw, vermiculite, pearlite, coffee grounds, grass residue, hemp fibre, and/or rock wool.
  • the present invention also relates to the use of a composition comprising cyclobutrifluram for growth of vesicular-arbuscular mycorrhizal fungi in a medium comprising a plant, the locus, or propagation material thereof, preferably while maintaining or improving the overall soil microbiome health, preserving soil biodiversity.
  • the present invention also relates to the use of a composition comprising cyclobutrifluram for improving plant wellness of plant s growing in a medium comprising the plant, the locus, or propagation material thereof, in the presence of a vesicular-arbuscular mycorrhizae fungal culture.
  • a medium is selected by identifying a portion thereof containing a plant pest present in an amount sufficient to harm or reduce the growth of a plant growing in the medium.
  • the method is applied where the plant pests include plant parasitic nematodes, in particular endoparasitic-, semi-endoparasitic- and ectoparasitic nematodes; and/or harmful bacteria or fungi, in particular of the genus Fusarium.
  • plant parasitic nematodes in particular endoparasitic-, semi-endoparasitic- and ectoparasitic nematodes
  • harmful bacteria or fungi in particular of the genus Fusarium.
  • seed and plant propagules embrace seeds and plant propagules of all kinds including but not limited to true seeds, seed pieces, suckers, corns, bulbs, fruit, tubers, grains, rhizomes, cuttings, cut shoots and the like and means in a preferred embodiment true seeds.
  • microbe herein relates to a microorganism, including but not limited to bacteria, archaebacteria, fungi, and algae, such as microaigae.
  • microbes are single-cellular organisms, for instance bacteria, cyanobacteria, some fungi, or some algae.
  • microbes includes multi-cellular organisms, such as certain fungi or algae, for instance multicellular filamentous fungi or multicellular algae.
  • a method for the use of the composition comprising the steps of: providing cyclobutrifluram or a composition comprising cyclobutrifluram as defined in any one of embodiments herein above: and applying the composition to a plant, propagation material, or the locus thereof.
  • Application according to the methods or uses according to the invention is preferably done to a crop or a plant, the locus thereof or propagation material thereof.
  • Preferably application is to a plant or propagation material thereof, more preferably to propagation material.
  • Application of cyclobutrifluram or a pesticidal composition comprising cyclobutrifluram may advantageously be performed according to any of the usual modes of application, e.g. foliar, drench, soil, in furrow or the like. Applicants found that due to the beneficial effect of the composition on soil microbiome composition over time, plant root growth could be significantly increased.
  • a method for the use of the composition comprising the steps of: providing cyclobutrifluram or a composition comprising cyclobutrifluram as defined in any one of embodiments herein above; and applying the composition to a propagation material; and planting of the propagation material into a medium, preferably a solid or terrestrial medium.
  • a method for growing a plant comprising applying or treating a propagation material thereof with cyclobutrifluram or a pesticidal composition comprising cyclobutrifluram as defined herein above, for the simultaneous or subsequent enhancement of beneficial microbial activity in the surrounding soil.
  • composition for maintaining or improving the overall soil microbiome health, preserving soil biodiversity.
  • the methods as defined in herein above are suitable for use on any plant, including those that have been genetically modified to be resistant to active ingredients such as herbicides, or to produce biologically active compounds that control infestation by plant pests.
  • cyclobutrifluram may be used in the form of a composition also referred to as a formulation, further containing a carrier.
  • Cyclobutrifluram and compositions comprising cyclobutrifluram as defined in any one of embodiments herein above may be used in various forms such as aerosol dispenser, capsule suspension, cold fogging concentrate, dust able powder, emulsifiable concentrate, emulsion oil in water, emulsion water in oil, encapsulated granule, fine granule, flowable concentrate for seed treatment, gas (under pressure), gas generating product, granule, hot fogging concentrate, macrogranule, microgranule, oil dispersible powder, oil miscible flowable concentrate, oil miscible liquid, paste, plant rodlet, powder for dry seed treatment, seed coated with a pesticide, soluble concentrate, soluble powder, solution for seed treatment, suspension concentrate (flowable concentrate), ultra low volume (ulv) liquid, ultra low volume (ulv) suspension, water dispersible granules or
  • a formulation typically comprises a liquid or solid carrier and optionally one or more customary formulation auxiliaries, which may be solid or liquid auxiliaries, for example unepoxidized or epoxidized vegetable oils, for example epoxidized coconut oil, rapeseed oil or soya oil; antifoaming agents, for example silicone oil, preservatives, clays, inorganic compounds, viscosity regulators, surfactant, binders and/or tackifiers.
  • auxiliaries for example unepoxidized or epoxidized vegetable oils, for example epoxidized coconut oil, rapeseed oil or soya oil
  • antifoaming agents for example silicone oil, preservatives, clays, inorganic compounds, viscosity regulators, surfactant, binders and/or tackifiers.
  • compositions for use in agriculture are emulsifiable concentrates, suspension concentrates, microemulsions, oil dispersibles, directly sprayable or dilutable solutions, spreadable pastes, dilute emulsions, soluble powders, dispersible powders, wettable powders, dusts, granules or encapsulations in polymeric substances, which comprise - at least - cyclobutrifluram and the type of composition is to be selected to suit the intended aims and the prevailing circumstances.
  • the compositions may comprise of from 0.1 to 99%, especially 0.1 to 95%, of cyclobutrifluram and 1 to 99.9%, especially 5 to 99.9%, of at least one solid or liquid carrier, it being possible as a rule for 0 to 25%, especially 0.1 to 20%, of the composition to be surfactants, % in each case meaning percent by weight.
  • foliar formulation types for pre-mix compositions are: GR: Granules; WP: wettable powders; WG: water dispersible granules (powders); SG: water soluble granules; SL: soluble concentrates; EC: emulsifiable concentrate; EW: emulsions, oil in water; M E: micro-emulsion; SC: aqueous suspension concentrate; CS: aqueous capsule suspension; OD: oil-based suspension concentrate, and SE: aqueous suspo-emulsion.
  • GR Granules
  • WP wettable powders
  • WG water dispersible granules (powders)
  • SG water soluble granules
  • SL soluble concentrates
  • EC emulsifiable concentrate
  • EW emulsions, oil in water
  • M E micro-emulsion
  • SC aqueous suspension concentrate
  • CS aqueous capsule suspension
  • Examples of seed treatment formulation types for pre-mix compositions are: WS: wettable powders for seed treatment; slurry LS: solution for seed treatment; ES: emulsions for seed treatment; FS: suspension concentrate for seed treatment; WG: water dispersible granules, and CS: aqueous capsule suspension.
  • Examples of formulation types suitable for tank-mix compositions are solutions, dilute emulsions, suspensions, or a mixture thereof, and dusts.
  • the methods of application such as foliar, drench, spraying, atomizing, dusting, scattering, coating or pouring, are chosen in accordance with the intended objectives and the prevailing circumstances.
  • the tank-mix compositions are generally prepared by diluting with a solvent (for example, water) the one or more pre-mix compositions containing different pesticides, and optionally further auxiliaries.
  • a solvent for example, water
  • the one or more pre-mix compositions containing different pesticides, and optionally further auxiliaries can be solid or liquid and are the substances ordinarily employed in form ulation technology, e.g. natural or regenerated m ineral substances, solvents, dispersants, wetting agents, tackifiers, thickeners, binders or fertilizers.
  • a tank-m ix formulation for foliar or soil application comprises 0.1 to 20%, especially 0.1 to 15 %, of the desired ingredients, and 99.9 to 80 %, especially 99.9 to 85 %, of a solid or liquid auxiliaries, including, for example, a solvent such as water, where the auxiliaries can be a surfactant in an amount of 0 to 20 %, especially 0.1 to 15 %, based on the tank-m ix formulation.
  • a pre-m ix formulation for foliar application comprises 0.1 to 99.9 %, especially 1 to 95 %, of the desired ingredients, and 99.9 to 0.1 %, especially 99 to 5 %, of a solid or liquid adjuvant (including, for example, a solvent such as water), where the auxiliaries can be a surfactant in an amount of 0 to 50 %, especially 0.5 to 40 %, based on the pre-m ix formulation.
  • a solid or liquid adjuvant including, for example, a solvent such as water
  • a tank-mix form ulation for seed treatment application comprises 0.25 to 80 %, especially 1 to 75 %, of the desired ingredients, and 99.75 to 20 %, especially 99 to 25 %, of a solid or liquid auxiliaries, including, for example, a solvent such as water, where the auxiliaries can be a surfactant in an amount of 0 to 40 %, especially 0.5 to 30 %, based on the tank-m ix formulation.
  • a pre-m ix form ulation for seed treatment application comprises 0.5 to 99.9 %, especially 1 to 95 %, of the desired ingredients, and 99.5 to 0.1 %, especially 99 to 5 %, of a solid or liquid adjuvant, including, for example, a solvent such as water, where the auxiliaries can be a surfactant in an amount of 0 to 50 %, especially 0.5 to 40 %, based on the pre-m ix form ulation.
  • pests of the abovementioned type which occur in particular on plants, especially on useful plants and ornamentals in agriculture, in horticulture and in forests, or on organs, such as fruits, flowers, foliage, stalks, tubers or roots, of such plants, and in some cases even plant organs which are formed at a later point in time remain protected against these pests.
  • Suitable target crops are, in particular, cereals, such as wheat, barley, rye, oats, rice, maize or sorghum ; beet, such as sugar or fodder beet; fruit, for example pomaceous fruit, stone fruit or soft fruit, such as apples, pears, plums, peaches, almonds, cherries or berries, for example strawberries, raspberries or blackberries; leguminous crops, such as beans, lentils, peas or soya; oil crops, such as oilseed rape, mustard, poppies, olives, sunflowers, coconut, castor, cocoa or ground nuts; cucurbits, such as pumpkins, cucumbers or melons; fibre plants, such as cotton, flax, hemp or jute; citrus fruit, such as oranges, lemons, grapefruit or tangerines; vegetables, such as spinach, lettuce, asparagus, cabbages, carrots, onions, tomatoes, potatoes or bell peppers;
  • Lauraceae such as avocado, Cinnamonium or camphor; and also tobacco, nuts, coffee, eggplants, sugarcane, tea, pepper, grapevines, hops, the plantain family and latex plants.
  • the methods of the present invention may be also used on any ornamental and/or vegetable crops, including flowers, shrubs, broad-leaved trees and evergreens.
  • the methods as defined in herein above are suitable for use on any plant, including those that have been genetically modified to be resistant to active ingredients such as herbicides, or to produce biologically active compounds that control infestation by plant pests.
  • composition may also further comprise a fertilizer, a micronutrient donor or other preparations which influence the growth of plants as well as comprising a combination containing the compound of the invention with one or more other biologically active agents, such as bactericides, fungicides, nematicides, plant activators, acaricides, and insecticides.
  • a fertilizer such as bactericides, fungicides, nematicides, plant activators, acaricides, and insecticides.
  • legum inous crops such as beans, lentils, peas, and/or soybeans.
  • differential abundance analysis of m icrobiome data detected no significant modifications in the bacterial com munity at genus level in connection with Cyclobutrifluram treatment.
  • ASV level no ecologically relevant ASVs (relative abundance > 1 %, Table 3a) were found significantly following cyclobutrifluram treatment.
  • the 14 ASVs found to be significantly changing are characterised by a relative abundance ⁇ 0.1 % and therefore of m inor ecological relevance.
  • Bacterial ASVs were detected by 16S rRNA gene amplicon sequencing with taxonomy assignment at ASV level.
  • Table 1a Table reporting bacterial ASVs
  • Acidobacteria-6 0.000 % 0.050 % Class + 0.050 %
  • Acidobacteria-6 0.000% 0.051 % Class +0.051 %
  • Acidobacteria-6 0.000 % 0.028 % Class + 0.028 %
  • Mycosphaerella 2.213% 1.672% -0.541 %
  • Candida 1.108 % 0.839 % - 0.269 %
  • Oonostachys 0.666 % 0.797 % + 0.132 %
  • Figure 1 shows the extent of the impact on various fungal taxa in direct comparison, both stimulated as well as inhibited.
  • Cyclobutrifluram treatment preserves soil microbiome diversity apart from inhibiting Fusarium, as the very negligible inhibiting or the stimulating effects to the other species have very low or no impacts from a microbial ecological perspective.
  • the location A plot is where the applied treatments were shown to have the greatest effect.
  • These treatments induced a significant increase in Pseudomonas putida, a plant growth-promoting rhizobacterium (PGPR) that possesses genes favouring nutrient mobilization, prevention of pathogen development, and efficient niche colonization.
  • PGPR plant growth-promoting rhizobacterium
  • Trichoderma species are a widely known group of ascomycetes which are considered to provide several benefits function, including a natural fungicide function, which provide protection to the plants to several pathogenic fungi.
  • Cyclobutrifluram treatment to counteract nematode infestation showed positive and preservative effects for soil microbiome diversity, resulting in a vibrant microbiome. Yet further, no significant negative effects of cyclobutrifluram were observed on the soil bacterial com munity of tomato plants in presence of RKN infestation in the medium-long term. Quite to the contrary, cyclobutrifluram treatment had a positive effect in the medium -long term on the soil fungal comm unity of tomato plants in presence of RKN infestation. In particular, the relative abundance of the plant-pathogen Fusarium was substantially reduced by the treatment with cyclobutrifluram.
  • compositions comprising cyclobutrifluram have shown conclusively to benefit the soil microbiome, by retaining the beneficial bacterial species diversity, and by suppressing pathogenic fungi, in particularly when compared to compositions comprising fluopyram.
  • Cyclobutrifluram treatment preserves soil biodiversity, and hence will preserve a vibrant range of life forms in the soil and enable plants to develop bigger root systems in infested farmlands.
  • a group of clyclobutrifluram analogues with high nematocidal activity and varying degrees of fungicidal activity against a diverse fungal and Oomycete disease spectrum was selected, based on a toxicity screens on nematodes (species Heterodera schachtii, Meloidogyne incognita) and fungal lineages including Ascomycota classes Dothideomycetes (species Zymoseptoria tritici, Cercospora arachidicola, Pyrenophora teres, Alternaria solani, Phaeosphaeria nodorum), Leotiomycetes (Erysiphe graminis, Erysiphe graminis, Botryotinia fuckeliana, Sclerotinia sclerotiorum), Sordariomycetes (Glomerella lagenarium, Fusarium Culmorum, Fusarium Culmorum
  • Soybeans of variety Toliman were treated with 0.15 mg ai / seed of cyclobutrifluram and analogues, and planted in 1.5 L pots of 0-3 mm sieved soil. The seedswere inoculated with Bradyrhizobium japonicum using a commercially available product for soybeans.
  • the plants were then grown in a growth chamber for 42 days with the following conditions: Temperature of 20°C and with a 12/ 12 hour day/ night.
  • Table 1 shows the structures of the compounds A1 to A7, as well as the impact of the different compounds on the mycorrhization in soybean roots. Surprisingly it was found that not only that cyclobutrifluram showed a higher colonization compared to the comparative compounds, but also vis-a-vis the control, while the tested analogues comparative examples, in spite of their structurally very closely relationship to cyclobutrifluram , exhibited a reduction on the level of colonization.
  • Table 1 Compounds tested for mycorrhizae compatibility; effect in soybean on the level of mycorrhization in soybean roots.
  • Cyclobutrifluram treatment showed very positive effects on the growth of arbuscular mycorrhizal fungi for soybeans, while also substantially supressing nematodes and undesired fungi.

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Abstract

Un procédé d'amélioration d'une caractéristique de plante d'une plante poussant dans un milieu,,,,,,,,,,,,, en présence d'une culture fongique de mycorhizes arbusculaires vésiculaires, consiste à appliquer une quantité efficace d'une composition comprenant du cyclobutriflurame, à la plante, au locus ou au milieu, ou à un matériau de propagation de plante.
EP23809590.5A 2022-11-24 2023-11-21 Procédés d'amélioration des caractéristiques d'une plante Pending EP4622460A1 (fr)

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