EP4661679A1 - Utilisation de biomasse finement broyée contenant de la sphaigne pour améliorer la croissance des plantes - Google Patents

Utilisation de biomasse finement broyée contenant de la sphaigne pour améliorer la croissance des plantes

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Publication number
EP4661679A1
EP4661679A1 EP24705550.2A EP24705550A EP4661679A1 EP 4661679 A1 EP4661679 A1 EP 4661679A1 EP 24705550 A EP24705550 A EP 24705550A EP 4661679 A1 EP4661679 A1 EP 4661679A1
Authority
EP
European Patent Office
Prior art keywords
finely milled
biomass
sphagnum moss
plant
milled biomass
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP24705550.2A
Other languages
German (de)
English (en)
Inventor
Teija HAKALAHTI
Kaisa HYTÖNEN
Katja HÄMÄLÄINEN
Antti PIETARI
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Neova Oy
Original Assignee
Neova Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Neova Oy filed Critical Neova Oy
Publication of EP4661679A1 publication Critical patent/EP4661679A1/fr
Pending legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P21/00—Plant growth regulators
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00—Fungicides
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/02—Other organic fertilisers from peat, brown coal, and similar vegetable deposits
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00—Soil-conditioning materials or soil-stabilising materials
    • C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
    • C09K17/18—Prepolymers; Macromolecular compounds
    • C09K17/32—Prepolymers; Macromolecular compounds of natural origin, e.g. cellulosic materials

Definitions

  • the present invention generally relates to uses of biomass in order to produce growth substrates and fertilizer supplements for plant cultivation.
  • the invention relates to the use of finely milled biomass comprising Sphagnum moss in agricultural applications, for example as a plant growth promoter or a biostimulant.
  • the invention also relates to growth substrates and fertilizers comprising finely milled Sphagnum biomass.
  • the invention also relates to a method for improving plant growth or plant development.
  • Sphagnum moss i.e. a plant genus incorporating several species (Sphagnum sp.), is a plant that grows on the top of peatlands such as bogs, forestry ditched areas, and other wetlands. Unlike peat, which is partially decayed vegetation of Sphagnum moss and other wetland plants, Sphagnum moss comprises living parts, particularly on the top layer of the vegetation, and is thus rapidly renewing material.
  • Sphagnum moss has been used in growing mediums.
  • the use of Sphagnum moss as a supplement in growing media has been justified by structural benefits the material offers to the growing medium or soil.
  • Sphagnum moss is known to improve airiness of the soil, or in dry soil types Sphagnum moss may help retaining moisture.
  • Sphagnum moss is also used as material for the production of greenhouse cultivation substrates. For instance, the use of Sphagnum moss for producing porous and solid growing medium structures is known from EP3048872.
  • FI128301 it is disclosed that Sphagnum moss, when air heated to a temperature within a range of 50-80 °C, possess pronounced suppressive activity against fungal diseases and moulds.
  • the authors of FI128301 disclose a plant disease-suppressive growing medium comprising air-dried Sphagnum moss biomass, preferably in the form of a fibrous particulate or a powder with particle size distribution of 0.1 - 2 mm.
  • Sphagnum moss is also known as an absorbent material.
  • US6890651 discloses finely milled Sphagnum moss or Sphagnum peat made of dry Sphagnum, dry Sphagnum moss or dry Sphagnum peat particles substantially having a size less than about 850 microns.
  • Plant growth promoters or biostimulants are substances that enhance the development of plants by direct or indirect means. They help in boosting the growth of the crop as well as its quality. The presence of different types of plant hormones such as cytokinins, gibberellins, and auxins in plant growth promoters helps in influencing better plant growth.
  • a use of finely milled biomass comprising Sphagnum moss for improving plant growth, wherein the particle size of said finely milled biomass is under 150 microns (pm), preferably the particle size is in the range of 0.2 - 100 microns.
  • a fertilizer or a plant growing medium comprising finely milled biomass essentially comprising Sphagnum moss for improving plant growth, wherein the particle size of said finely milled biomass is under 150 microns (pm), preferably the particle size is in the range of 0.2 - 100 microns.
  • a method for improving plant growth or plant development comprising a step of applying finely milled biomass comprising Sphagnum moss to a plant, growing medium, or soil where the plant is cultivated, wherein the particle size of the finely milled biomass is under 150 microns (pm), preferably the particle size is in the range of 0.2 - 100 microns.
  • FIGURE 1 Disease index of Rhizoctonia solani infected potato tubers. Data presented are the mean ⁇ standard deviation for 6 replicates. [0013] FIGURE 2. Diameter of fungal colonies three days after the beginning of the experiment. Black bars indicate the growth of Fusarium graminearum and grey bars indicate the growth of Rhizoctonia solani. Neg. control: Milli-Q water; pos. control: Amistar-fungicide.
  • FIGURE 3 Dry aerial biomass ( ⁇ SD) of lettuce in different treatments.
  • the experimental set up consisted of control treatment without Sphagnum moss. In other treatments, different Sphagnum products were applied on the growing media. Drought stress was applied for all of the treatments by reducing watering by 50 % of normal watering regime for lettuce. Each treatment consisted of 6 replicates with 6 plants in each. Different treatments differ statistically significantly (LSD statistical analysis, p ⁇ 0.05), if they do not share the same letter.
  • Sphagnum moss means the plant material originating from the upper layers (acrotelm) of peatland biomass (preferably a depth of 10 - 70 cm from the surface) mainly including different Sphagnum moss species (Sphagnum spp.).
  • Sphagnum spp. The most common Sphagnum moss species in such material are S. papillosum, S. fuscum, S. magellanicum, S. cuspidata spp. and S. riparium.
  • Said layer will also likely comprise some other living peatland plants (e.g.
  • vascular and non-vascular plants like Carex spp., Calluna vulgaris, Eriophorum vaginatum, Empetrum nigum, Trichoporum spp., Empetrum nigrum, Andromeda polifolia, Pinus sylvestris, Rubus chamaemorus, and Drosera spp.).
  • the methods for harvesting Sphagnum moss from bogs are well-known (see, e.g., FI128608).
  • plant growth refers to the growth of any plant part, including stems, leaves, flowers, inflorescences, fruits, and roots. Growth may refer to the rate of growth of any one of these plant parts.
  • plant development as understood herein is the process by which structures originate and mature as a plant grows. Plants produce new tissues and structures throughout their life from clusters of undifferentiated cells called meristems.
  • biostimulant refers to a substance or microorganism applied to plants with the aim to enhance nutrient use efficiency, abiotic stress tolerance and/or crop quality traits, regardless of its nutrient content.
  • biocontrol refers to a method of controlling plant diseases caused by bacteria or fungi using other bacteria and fungi capable of reducing disease occurrence.
  • finely milled biomass essentially comprising Sphagnum moss and having particle size under 150 pm provides growth promoting effects with several crop plants.
  • said finely milled biomass also has biocontrol effect (i.e. a fungicidal effect) on fungal plant pathogens such as Rhizoctonia solani.
  • the milling process as described in this disclosure results in breakage of the fibers and cell structures of the Sphagnum moss cells and releases active ingredients i.e. growth promoting and/or biostimulant extracts from the cell structures.
  • active ingredients i.e. growth promoting and/or biostimulant extracts from the cell structures.
  • These fibers, cell structures and extracts preferably remain active even when dried (preferably at least to a moisture content of 10 - 15 % w/w) for preservation purposes and subsequently used as a fertilizer supplement.
  • Sphagnum moss cells are known to comprise endosymbiotic microbes, such as bacteria, which may also be released in the milling treatment and may provide part of the plant growth promoting, or biocontrolling effect of the finely milled composition.
  • the present invention is thus directed to a use of finely milled biomass comprising Sphagnum moss for improving plant growth, wherein the particle size of said finely milled biomass is under 150 microns (pm), preferably under 125, 120, 115, 110, or under 100 microns, preferably the particle size is in the range of 0.2 - 100 microns, more preferably in the range of 1 - 120, 1 - 100, 10 - 100, 50 - 100, 80 - 100, 80 - 125, or 80 - 110 microns. Other suitable ranges are 1 - 95, 10 - 95, or 50 - 95 microns.
  • said finely milled biomass is in solid form or contained in an aqueous suspension (i.e. the solvent is water).
  • said aqueous suspension comprises at least 0.02% (w/v), 0.1% (w/v), 0.5% (w/v), 1% (w/v), 2% (w/v), 5% (w/v), 10% (w/v), 20% (w/v), 30% (w/v), 40% (w/v), 50% (w/v), 60% (w/v), or 70% (w/v), or up to 0.5% (w/v), 1% (w/v), 2% (w/v), 5% (w/v), 10% (w/v), 20% (w/v), 30% (w/v), 40% (w/v), 50% (w/v), 60% (w/v), or 70% (w/v) in dry weight of said finely milled biomass.
  • said finely milled biomass improves plant stem growth, leaf growth, root growth, and/or stress tolerance.
  • said finely milled Sphagnum moss is not heat- treated in temperatures over 50 °C, particularly in the milling or drying stages during the manufacturing process of the finely milled composition.
  • the present invention is also directed to a fertilizer or a plant growing medium comprising finely milled biomass essentially comprising Sphagnum moss as a plant growth promoter or biostimulant, wherein the particle size of said finely milled biomass is under 150 microns (pm), preferably the particle size is in the range of 0.2 - 100 microns.
  • said fertilizer is an aqueous suspension comprising at least 0.02% (w/v), 0.1% (w/v), 0.5% (w/v), 1% (w/v), 2% (w/v), 5% (w/v), 10% (w/v), 20% (w/v), 30% (w/v), 40% (w/v), 50% (w/v), 60% (w/v), or 70% (w/v), or up to 0.5% (w/v), 1% (w/v), 2% (w/v), 5% (w/v), 10% (w/v), 20% (w/v), 30% (w/v), 40% (w/v), 50% (w/v), 60% (w/v), or 70% (w/v) in dry weight of said finely milled biomass.
  • the present invention is also directed to a method for improving plant growth or plant development comprising a step of applying finely milled biomass comprising Sphagnum moss to a plant, growing medium, or soil where the plant is cultivated, wherein the particle size of the finely milled biomass is under 150 microns (pm), preferably the particle size is in the range of 0.2 - 100 microns.
  • the present disclosure relates to a method for the production of finely milled Sphagnum moss, wherein the method comprises at least the steps of: a) selecting raw material comprising Sphagnum moss, preferably based on its moisture content, which should be in the range of 5 to 95 %; b) subjecting the selected raw material to a single or multistage milling process, preferably by disc or conical milling between a rotor and a stator, between counter-rotating rotors, in a ball or pearl mill, or dispersion in a vessel or a tank, in a temperature range of 5 to 80 °C, preferably under 50 °C, and in a pressure range of 1 bar(a) to 10 bar(a); c) subjecting said finely milled Sphagnum moss to a drying process, such as vacuum drying or freeze drying, in a temperature range of -50 to 200 °C, preferably under 50 °C.
  • the temperature of the drying process is more preferably in the
  • the milling step b) in the above method can be carried out by any suitable grinding method and devices, e.g. by cavitation, by wet grinding, such as by ball mill or pearl mill, or by any kind of other grinder that can produce fine particles.
  • the grinding method can be selected from the group consisting of crushing-based grinding, wet grinding, dry grinding, grinding under pressure, other suitable grinding method and their combinations.
  • the grinding device used for grinding is selected from the group consisting of sand mill, bead mill, pearl mill, ball mill, vibration mill, screw mill, extrusion device, other suitable device and their combinations. Modifications of the milling processes are within the skill of one of ordinary skill in the art.
  • the drying step c) in the above method can be carried out by any suitable drying method and devices.
  • the drying can be made by means of drying device selected from the group: freeze dryer, vacuum dryer, air dryer, air grinder, rotor mill, centrifugal mill, air turbulence mill, air turbulence dryer and other suitable dryers and other suitable mills, and their combinations.
  • Exemplary crop plants include, but are not limited to, tubers and other below-ground vegetables (such as potatoes, beetroots, radishes, carrots, onions, etc.), cereals (such as rice, maize, wheat, barley, rye etc.), leaf vegetables (such as lettuces, chard, spinach etc.), and other vegetables (such as tomatoes).
  • tubers and other below-ground vegetables such as potatoes, beetroots, radishes, carrots, onions, etc.
  • cereals such as rice, maize, wheat, barley, rye etc.
  • leaf vegetables such as lettuces, chard, spinach etc.
  • other vegetables such as tomatoes.
  • the finely milled biomass as disclosed herein may be applied directly to plants, plant parts (such as foliage), or seeds, or alternatively may be applied to soil or growth substrate in which the plants are growing or to be grown or in which seeds have been or are to be sown or planted.
  • Application may be by any suitable means and may be on any suitable scale.
  • application may comprise pouring, spreading or spraying, including broad scale or bulk spreading or spraying, soaking of seeds before planting, and/or drenching of seeds after planting or seedlings.
  • multiple means of application may be used in combination (for example soaking of seeds prior to planting followed by drenching of planted seeds and/or application to seedlings or mature plants).
  • the finely milled biomass compositions as disclosed herein and fertilizers comprising said compositions may be prepared in any suitable form depending on the means by which the finely milled biomass composition is to be applied to the soil, growth substrate, or to plant seeds or vegetation.
  • suitable forms can include, for example, slurries, liquids, and solid forms.
  • Solid forms include powders, granules, larger particulate forms and pellets.
  • Eiquids may include aqueous solutions and aqueous suspensions, and emulsifiable concentrates.
  • Sphagnum moss material Sphagnum suspensions were made of mechanically collected Sphagnum moss from which water was squeezed out.
  • the main composition of the collected material was Sphagnum (about 80-90%, mainly containing S. papillosum and S. cuspidata) and also including other bog vegetation, mainly hare's-tail cottongrass (Eriophorum vaginatum).
  • the collected moss was pre-milled with a plate grinder, and when grinding, water was added to the mass to prevent heating.
  • the pre-milled material was then ground with a pearl mill and Masuko grinder. No preservative was added to the product and the product was stored in a cool place (+ 4 C°) after grinding.
  • EXAMPLE 1 Investigating Sphagnum suspension’s growth-promoting effect to Arabidopsis thaliana fresh roots and fresh aerial biomass, in vitro.
  • the trials were performed in square Petri dishes in sterile conditions. Arabidopsis thaliana seeds were sown on growing medium (impoverished nutrition medium). After 7 days, the most homogenous plantlets were transplanted on the same growing medium. The trials were organized in three independent replicates of one Petri dish per treatment. Each Petri dish contained 8 plantlets. Product samples (Sphagnum suspension with median particle size of 1 pm, 10 pm or 100 pm) were applied with a pipette (50pl/plant) on the whole plant 11 days after sowing. Three different Sphagnum suspension concentrations were tested: 0.02%, 0.2% and 2% of dry matter.
  • Evaluated treatments were compared to each other and into two control treatments: a negative control treated with water and a positive control treated with a nutrient solution.
  • the evaluation of product’s efficacy was performed at the end of each test (24 days from sowing) by weighing fresh aerial, fresh root and fresh total biomass of each plant. Mean value for each replicate Petri dish was used in statistical testing. Analysis of variance (ANOVA) was used in the data analysis. LSD test was used in comparison of different treatments.
  • Table 1 Arabidopsis thaliana fresh aerial, root and total biomass (mg). Data presented are the mean ⁇ standard deviation for the three replicates. Letters correspond to homogenous group after LSD statistical analysis. Different treatments differ statistically significantly (p ⁇ 0.05), if they do not share the same letter code (a, b, c). Table 2. Arabidopsis thaliana aerial, root and total biomass (mg). Data presented are the mean ⁇ standard deviation for the three replicates. Letters correspond to homogenous group after LSD statistical analysis. Different treatments differ statistically significantly (p ⁇ 0.05), if they do not share the same letter code (a, b, c).
  • EXAMPLE 2 Effect of Sphagnum suspension on root development of radish in pots in a phytotron.
  • Reduced based fertilization was also applied into growing medium, where Sphagnum suspension was added. No additional fertilization was given during the experiment in any of the treatments.
  • the evaluation of product’s efficacy was performed after 31 days from the sowing when first symptoms of nutrient deficiency was observed in the plants. Roots were evaluated visually with scoring 1 (few roots) to 5 (many roots). Analysis of variance (ANOVA) was used in the data analysis. LSD test was used in comparison of different treatments.
  • EXAMPLE 3 Effect of Sphagnum suspension on stomatai conductance of salt stressed tomato in pots in a greenhouse.
  • Evaluated treatment was compared into two control treatments: negative control where watering was performed with fresh water (no stress) and positive control where watering was performed with salted water (stress). The stress was followed up by monitoring soil conductivity and humidity. The evaluation of product's efficacy was performed at the end of the tests (2 months from sowing) by measuring stomatai conductance in different leaf stages: leaf stage 4, leaf stage 8 and last developed leaf. Measurement was performed by using a porometer. Kruskal- Wallis test was used in the data analysis. LSD test was used in comparison of different treatments.
  • Leaf stomata of plants can be closed under stress conditions. Under salinity stress the stomatai conductance is severely affected, thereby influencing photosynthetic efficiency of plants and hindering growth and yield. The leaf stomatai conductance was higher in positive control treatment without the salt stress compared to any other treatments (Table 4). There is a trend that tomatoes treated with Sphagnum suspensions (10 pm or 100 pm) had higher stomatai conductance than plants in salt stressed control when measure on the 4 th leaf stage and on last developed leaf stage. This indicates that tomatoes treated with Sphagnum suspension products were able to scope from salt stress better than those that were only exposed to salt stress.
  • Table 4 Stomatai conductance (mmol/m 2 /s) of tomatoes in different leaf stages. Data presents the mean value of stomatai conductance ⁇ standard deviation for each treatment. Letters correspond to homogenous group after LSD statistical analysis. Different treatments differ statistically significantly (p ⁇ 0.05), if they do not share the same letter.
  • EXAMPLE 4 Evaluation of the efficacy of Sphagnum suspension against Rhizoctonia solani on potato, compared to a commercial fungicide Amistar.
  • Rhizoctonia solani fungal pathogen
  • Tubers were planted in growing medium (EGO 120 standard coco, Jiffy substrates) on 4 litres pot and raised in greenhouse for 4 months until tubers production and harvest.
  • Fertilizer was mixed with the growing medium at 20 g/pot (Horticote 16 N - 2.6 P - 9.1 K - 1.2 Mg + TE).
  • Rhizoctonia solani inoculum was obtained by multiplication of the fungi on millet. Infected millet was added and mixed to the growing medium. Treatment was performed by using soil application.
  • the quantity of product (Sphagnum suspension, particle size 10 pm) was diluted in 200 ml of water. About 10 ml of this solution was sprayed on potato tuber. The remaining solution (about 180 ml) was mixed with the growing medium before tuber planting.
  • the level of disease development on tuber was evaluated using a standardized scale of disease index (DI): - 0 : no symptom, - 1 : ⁇ 1 % of the tuber covered by sclerotia, - 3 : ⁇ 4 % of the tuber covered by sclerotia, - 5 : ⁇ 9 % of the tuber covered by sclerotia, - 7 : ⁇ 14 % of the tuber covered by sclerotia. Evaluated treatment was compared to two control treatments: a negative control treated with water and a positive control treated with a commercial fungicide Amistar applied according to manufacturer’s instructions.
  • DI standardized scale of disease index
  • Antifungal activity of Sphagnum suspension was tested under laboratory conditions by using potato dextrose agar (PDA) (39 g/1 70139, Merck KGaA, Darmastadt, Germany) and two different fungal isolates. PDA was autoclaved 121 °C for 15 minutes and cooled to 60 °C. PDA was supplemented with the Sphagnum suspension with final concentrations of 1 % of dry matter. Ultrapure Milli-Q water was used as negative control and Amistar fungicide (Syngenta, Basel, Switzerland) as positive control according to manufacturer’s instructions. Antifungal activity of Sphagnum suspension was tested against Fusarium graminearum Schwabe ja Rhizoctonia solani J. G. Kuhn.
  • the trial aimed to evaluate the efficacy of Sphagnum-based products on lettuce (Kitsch variety) yield under water stress. It was tested, if there is difference in growth results between typical growing media Sphagnum and finely milled Sphagnum suspensions under drought stress.
  • the particle size of common growing media Sphagnum (not milled) was > 3 mm.
  • the median particle sizes (d50) of Sphagnum in milled Sphagnum suspensions were 51.6 or 88.7 micrometers.
  • the experimental set up consisted of control treatment without Sphagnum moss. In other treatments, different Sphagnum products were applied on the growing media. Drought stress was applied for all of the treatments by reducing watering by 50 % of normal watering regime for lettuce. Each treatment consisted of 6 replicates with 6 plants in each.
  • Base growing media used in the test consisted of fine light peat 60 %, black peat 40 % and pH modifier. The dosing of each Sphagnum product with 85 % dry matter was 50 % of the volume of the growing media. For one of the products also lower dosing (5 or 20 % of volume) were tested. Seeds were sown in each test growing media and after 30 days from the start of the trial plant biomass was measured to evaluate the yield in different treatments.

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Abstract

La présente invention concerne une nouvelle utilisation de biomasse finement broyée contenant de la sphaigne pour améliorer la croissance des plantes, la taille des particules de ladite biomasse finement broyée étant inférieure à 150 microns (µm), de préférence dans la plage allant de 0,2 à 100 microns. La présente invention concerne également un engrais ou un milieu de culture de plantes comprenant une biomasse finement broyée contenant essentiellement de la sphaigne. La présente invention concerne en outre un procédé d'amélioration de la croissance de plantes ou du développement de plantes comprenant une étape consistant à appliquer une biomasse finement broyée contenant de la sphaigne sur une plante, un milieu de culture ou un sol où la plante est cultivée.
EP24705550.2A 2023-02-10 2024-02-09 Utilisation de biomasse finement broyée contenant de la sphaigne pour améliorer la croissance des plantes Pending EP4661679A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI20235138A FI20235138A1 (en) 2023-02-10 2023-02-10 Use of finely ground biomass comprising Sphagnum moss to enhance plant growth
PCT/FI2024/050051 WO2024165794A1 (fr) 2023-02-10 2024-02-09 Utilisation de biomasse finement broyée contenant de la sphaigne pour améliorer la croissance des plantes

Publications (1)

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EP4661679A1 true EP4661679A1 (fr) 2025-12-17

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EP24705550.2A Pending EP4661679A1 (fr) 2023-02-10 2024-02-09 Utilisation de biomasse finement broyée contenant de la sphaigne pour améliorer la croissance des plantes

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Country Link
EP (1) EP4661679A1 (fr)
FI (1) FI20235138A1 (fr)
WO (1) WO2024165794A1 (fr)

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US20060032124A1 (en) * 2004-04-14 2006-02-16 Knighton David R Methods of inhibiting microorganism growth using moss
FI125943B (en) 2013-09-26 2016-04-15 Teknologian Tutkimuskeskus Vtt Oy Plant substrate structures based on white moss and their method of production
RU2608527C2 (ru) * 2015-06-17 2017-01-19 Публичное акционерное общество "Газпром" Биокомпозитный материал для очистки сточных вод от нитрит-, нитрат-, фосфат-ионов
FI128301B (en) 2017-05-08 2020-03-13 Luonnonvarakeskus A method of stimulating the anti-herbal function in the moss, associated products and uses
FI128608B (fi) 2019-06-28 2020-08-31 Vapo Oy Menetelmä ja laite veden poistamiseksi rahkasammaleesta sekä työkone rahkasammaleen nostamiseksi ja kuivaamiseksi

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