EP4702138A1 - Production d'un oligosaccharide chargé négativement par une cellule - Google Patents

Production d'un oligosaccharide chargé négativement par une cellule

Info

Publication number
EP4702138A1
EP4702138A1 EP24726545.7A EP24726545A EP4702138A1 EP 4702138 A1 EP4702138 A1 EP 4702138A1 EP 24726545 A EP24726545 A EP 24726545A EP 4702138 A1 EP4702138 A1 EP 4702138A1
Authority
EP
European Patent Office
Prior art keywords
gal
neu5ac
oligosaccharide
glcnac
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP24726545.7A
Other languages
German (de)
English (en)
Inventor
Joeri Beauprez
Thomas DECOENE
Annelies VERCAUTEREN
Tom Verhaeghe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Inbiose NV
Original Assignee
Inbiose NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inbiose NV filed Critical Inbiose NV
Priority claimed from PCT/EP2024/061529 external-priority patent/WO2024223815A1/fr
Publication of EP4702138A1 publication Critical patent/EP4702138A1/fr
Pending legal-status Critical Current

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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
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    • C12P19/00—Preparation of compounds containing saccharide radicals
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
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    • C12R2001/15—Corynebacterium
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae
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    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01183—UDP-N-acetylglucosamine 2-epimerase (hydrolysing) (3.2.1.183)

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
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  • Engineering & Computer Science (AREA)
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  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La présente invention relève du domaine technique de la biologie synthétique, de l'ingénierie métabolique et de la culture cellulaire. L'invention propose une cellule pour la production d'un oligosaccharide chargé négativement, de préférence sialylaté, ladite cellule étant génétiquement modifiée pour posséder ou exprimer, de préférence pour surexprimer une UDP-N-acétyl-D-glucosamine-2-épimérase d'hydrolyse. L'invention propose en outre l'utilisation de ladite cellule dans une culture ou une incubation. L'invention décrit également des procédés de production d'un oligosaccharide chargé négativement, de préférence sialylaté, à l'aide de ladite cellule ainsi que la purification dudit oligosaccharide chargé négativement, de préférence sialylaté.
EP24726545.7A 2023-04-26 2024-04-26 Production d'un oligosaccharide chargé négativement par une cellule Pending EP4702138A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP23169954 2023-04-26
LU504075 2023-04-26
PCT/EP2024/061529 WO2024223815A1 (fr) 2023-04-26 2024-04-26 Production d'un oligosaccharide chargé négativement par une cellule

Publications (1)

Publication Number Publication Date
EP4702138A1 true EP4702138A1 (fr) 2026-03-04

Family

ID=97837057

Family Applications (1)

Application Number Title Priority Date Filing Date
EP24726545.7A Pending EP4702138A1 (fr) 2023-04-26 2024-04-26 Production d'un oligosaccharide chargé négativement par une cellule

Country Status (4)

Country Link
EP (1) EP4702138A1 (fr)
KR (1) KR20260005332A (fr)
CN (1) CN121079401A (fr)
AU (1) AU2024264279A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN121496115A (zh) * 2026-01-08 2026-02-10 内蒙古金达威药业有限公司 一种软骨素发酵生产控制方法

Also Published As

Publication number Publication date
AU2024264279A1 (en) 2025-12-11
CN121079401A (zh) 2025-12-05
KR20260005332A (ko) 2026-01-09

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