EP4705516A1 - Procédé et système pour améliorer la spécificité de détection d'analyte à l'aide d'une amplification d'acide nucléique en temps réel - Google Patents

Procédé et système pour améliorer la spécificité de détection d'analyte à l'aide d'une amplification d'acide nucléique en temps réel

Info

Publication number
EP4705516A1
EP4705516A1 EP24733361.0A EP24733361A EP4705516A1 EP 4705516 A1 EP4705516 A1 EP 4705516A1 EP 24733361 A EP24733361 A EP 24733361A EP 4705516 A1 EP4705516 A1 EP 4705516A1
Authority
EP
European Patent Office
Prior art keywords
nucleic acid
computer
target nucleic
real
derivative
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP24733361.0A
Other languages
German (de)
English (en)
Inventor
Vincent Chabot
David Opalsky
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gen Probe Inc
Original Assignee
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Publication of EP4705516A1 publication Critical patent/EP4705516A1/fr
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/15—Preventing contamination of the components of the optical system or obstruction of the light path
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B1/00—Cleaning by methods involving the use of tools
    • B08B1/10—Cleaning by methods involving the use of tools characterised by the type of cleaning tool
    • B08B1/14—Wipes; Absorbent members, e.g. swabs or sponges
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
    • B08B9/08—Cleaning containers, e.g. tanks
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/24—Coupling light guides
    • G02B6/36—Mechanical coupling means
    • G02B6/38—Mechanical coupling means having fibre to fibre mating means
    • G02B6/3807—Dismountable connectors, i.e. comprising plugs
    • G02B6/3833—Details of mounting fibres in ferrules; Assembly methods; Manufacture
    • G02B6/3866—Devices, tools or methods for cleaning connectors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B2240/00—Type of materials or objects being cleaned
    • B08B2240/02—Optical fibers or optical fiber connectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/15—Preventing contamination of the components of the optical system or obstruction of the light path
    • G01N2021/152—Scraping; Brushing; Moving band
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0401—Sample carriers, cuvettes or reaction vessels
    • G01N2035/0429—Sample carriers adapted for special purposes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0401—Sample carriers, cuvettes or reaction vessels
    • G01N2035/0437—Cleaning cuvettes or reaction vessels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0099—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Mechanical Engineering (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Optics & Photonics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des procédés et des systèmes comprenant des ordinateurs programmés qui peuvent être utilisés pour améliorer la détection d'analyte d'acide nucléique à l'aide d'une amplification et d'une surveillance en temps réel, où des séquences d'acide nucléique étroitement associées peuvent être résolues. Dans un mode de réalisation, la détection de séquences cibles différentes les unes des autres par un seul nucléotide a été résolue par application d'une transformation mathématique. Dans un autre mode de réalisation, deux séquences cibles différant l'une de l'autre par un seul nucléotide ont été détectées et résolues à l'aide d'une sonde marquée spécifique pour une seule des deux séquences cibles.
EP24733361.0A 2023-05-05 2024-05-03 Procédé et système pour améliorer la spécificité de détection d'analyte à l'aide d'une amplification d'acide nucléique en temps réel Pending EP4705516A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363500475P 2023-05-05 2023-05-05
PCT/US2024/027803 WO2024233375A1 (fr) 2023-05-05 2024-05-03 Procédé et système pour améliorer la spécificité de détection d'analyte à l'aide d'une amplification d'acide nucléique en temps réel

Publications (1)

Publication Number Publication Date
EP4705516A1 true EP4705516A1 (fr) 2026-03-11

Family

ID=92801177

Family Applications (1)

Application Number Title Priority Date Filing Date
EP24733361.0A Pending EP4705516A1 (fr) 2023-05-05 2024-05-03 Procédé et système pour améliorer la spécificité de détection d'analyte à l'aide d'une amplification d'acide nucléique en temps réel

Country Status (3)

Country Link
EP (1) EP4705516A1 (fr)
AU (1) AU2024267362A1 (fr)
WO (1) WO2024233375A1 (fr)

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4868105A (en) 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
ATE88762T1 (de) 1986-08-11 1993-05-15 Siska Diagnostics Inc Methoden und zusammensetzungen fuer tests mit nukleinsaeuresonden.
CA1340843C (fr) 1987-07-31 1999-12-07 J. Lawrence Burg Amplification selective de sequences polynucleotidiques cibles
US5124246A (en) 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
WO1989009835A1 (fr) 1988-04-08 1989-10-19 The Salk Institute For Biological Studies Procede d'amplification a base de ligase
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
JP3276955B2 (ja) 1988-09-30 2002-04-22 ジーン−トラック・システムス Ｒｎａの鋳型の末端に結合したプローブ構造およびその使用方法
CA2020958C (fr) 1989-07-11 2005-01-11 Daniel L. Kacian Methodes d'amplification de sequences d'acide nucleique
US5128068A (en) 1990-05-25 1992-07-07 Westinghouse Electric Corp. Method and apparatus for cleaning contaminated particulate material
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
ATE257860T1 (de) 1991-08-02 2004-01-15 Biomerieux Bv Quantifikation von nukleinsäuren
JP4137996B2 (ja) 1992-05-06 2008-08-20 ジェン−プローブ・インコーポレイテッド 核酸配列増幅方法，組成物及びキット
CA2141430C (fr) 1992-08-04 2009-05-12 Sherrol H. Mcdonough Amplification d'une sequence d'acide nucleique
WO1995003430A1 (fr) 1993-07-23 1995-02-02 Gen-Probe Incorporated Procede d'amelioration de l'amplification de l'acide nucleique
ATE282473T1 (de) 1997-05-02 2004-12-15 Gen Probe Inc Reaktion behälter apparat
DE10011529T1 (de) 1998-05-01 2011-09-01 Gen-Probe Incorporated Automatisches Diagnoseanalysegerät und Verfahren
US8337753B2 (en) 1998-05-01 2012-12-25 Gen-Probe Incorporated Temperature-controlled incubator having a receptacle mixing mechanism
US6303305B1 (en) * 1999-03-30 2001-10-16 Roche Diagnostics, Gmbh Method for quantification of an analyte
US6783934B1 (en) * 2000-05-01 2004-08-31 Cepheid, Inc. Methods for quantitative analysis of nucleic acid amplification reaction
CA2898814C (fr) 2004-08-27 2015-12-29 Gen-Probe Incorporated Techniques d'amplification d'acide nucleique avec une seule amorce
US7964413B2 (en) 2005-03-10 2011-06-21 Gen-Probe Incorporated Method for continuous mode processing of multiple reaction receptacles in a real-time amplification assay
EP3663004B1 (fr) 2009-05-15 2022-01-26 Gen-Probe Incorporated Procédé et appareil pour la réalisation d'une procédure de séparation magnétique
US8309036B2 (en) 2009-05-15 2012-11-13 Gen-Probe Incorporated Method for separating viscous materials suspended from a pipette
EP2430458B1 (fr) 2009-05-15 2017-04-19 Gen-Probe Incorporated Procédé et appareil pour effectuer un transfert de récipients de réaction dans un instrument pour procédures analytiques à étapes multiples
EP2678664B1 (fr) 2011-02-24 2019-08-07 Gen-Probe Incorporated Systèmes et procédés de distinction de signaux optiques de différentes fréquences de modulation dans un détecteur de signaux optiques
AU2013202808B2 (en) 2012-07-31 2014-11-13 Gen-Probe Incorporated System and method for performing multiplex thermal melt analysis
US10176293B2 (en) * 2012-10-02 2019-01-08 Roche Molecular Systems, Inc. Universal method to determine real-time PCR cycle threshold values
AU2013202788B2 (en) 2013-03-14 2015-10-01 Gen-Probe Incorporated Indexing signal detection module
AU2013202805B2 (en) 2013-03-14 2015-07-16 Gen-Probe Incorporated System and method for extending the capabilities of a diagnostic analyzer
AU2013202782B2 (en) 2013-03-14 2015-05-14 Gen-Probe Incorporated Apparatus for indexing and agitating fluid containers
KR20160039529A (ko) * 2014-10-01 2016-04-11 주식회사 씨젠 증폭곡선을 보정하는 방법 및 시료 분석을 위한 정보를 획득하는 방법, 그 기록매체, 그 컴퓨터 프로그램, 컴퓨터 장치
EP3442710B1 (fr) 2016-04-14 2024-11-13 Gen-Probe Incorporated Ensembles pour le stockage de consommables de traitement d'échantillons et instruments de traitement d'échantillons
WO2019168261A1 (fr) * 2018-02-28 2019-09-06 (주)제노텍 Procédé d'analyse qualitative ou quantitative de génotype mutant et kit de pcr en temps réel pour la mise en œuvre de ce procédé
WO2020014400A1 (fr) * 2018-07-10 2020-01-16 Gen-Probe Incorporated Procédés et systèmes de détection et de quantification d'acides nucléiques
AU2021308095A1 (en) 2020-07-17 2023-03-09 Gen-Probe Incorporated Detection of macrolide-resistant mycoplasma genitalium

Also Published As

Publication number Publication date
WO2024233375A1 (fr) 2024-11-14
AU2024267362A1 (en) 2025-11-13

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