EP4709742A1 - Hélicases modifiées - Google Patents

Hélicases modifiées

Info

Publication number
EP4709742A1
EP4709742A1 EP24729726.0A EP24729726A EP4709742A1 EP 4709742 A1 EP4709742 A1 EP 4709742A1 EP 24729726 A EP24729726 A EP 24729726A EP 4709742 A1 EP4709742 A1 EP 4709742A1
Authority
EP
European Patent Office
Prior art keywords
helicase
modified
polynucleotide
length
pore
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP24729726.0A
Other languages
German (de)
English (en)
Inventor
Matthew Robertson HERBERT
Elizabeth Ann SNELL
Richard Charles FOSTER
Rebecca Victoria BOWEN
Mark John BRUCE
Joseph Hargreaves LLOYD
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oxford Nanopore Technologies PLC
Original Assignee
Oxford Nanopore Technologies PLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oxford Nanopore Technologies PLC filed Critical Oxford Nanopore Technologies PLC
Publication of EP4709742A1 publication Critical patent/EP4709742A1/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Hematology (AREA)
  • General Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Pathology (AREA)
  • Nanotechnology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des hélicases modifiées capables de réguler le mouvement de polynucléotides à vitesse accrue. Les hélicases modifiées sont particulièrement utiles pour le séquençage de polynucléotides.
EP24729726.0A 2023-05-12 2024-05-13 Hélicases modifiées Pending EP4709742A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB2307141.8A GB202307141D0 (en) 2023-05-12 2023-05-12 Modified helicases
PCT/EP2024/063118 WO2024235919A1 (fr) 2023-05-12 2024-05-13 Hélicases modifiées

Publications (1)

Publication Number Publication Date
EP4709742A1 true EP4709742A1 (fr) 2026-03-18

Family

ID=86872448

Family Applications (1)

Application Number Title Priority Date Filing Date
EP24729726.0A Pending EP4709742A1 (fr) 2023-05-12 2024-05-13 Hélicases modifiées

Country Status (4)

Country Link
EP (1) EP4709742A1 (fr)
CN (1) CN121100125A (fr)
GB (1) GB202307141D0 (fr)
WO (1) WO2024235919A1 (fr)

Family Cites Families (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
WO2008102120A1 (fr) 2007-02-20 2008-08-28 Oxford Nanopore Technologies Limited Système de capteur bicouche lipidique
EP3543357A1 (fr) 2007-05-08 2019-09-25 Trustees of Boston University Fonctionnalisation chimique de nanopores à l'état solide et de réseaux de nanopores et leurs applications
US8698481B2 (en) 2007-09-12 2014-04-15 President And Fellows Of Harvard College High-resolution molecular sensor
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
WO2010004265A1 (fr) 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Constructions enzyme-pore
US20120100530A1 (en) 2009-01-30 2012-04-26 Oxford Nanopore Technologies Limited Enzyme mutant
BRPI1012752B1 (pt) 2009-04-20 2019-06-25 Oxford Nanopore Technologies Limited Método e aparelho para detectar uma interação de uma entidade molecular com uma proteína de membrana em uma camada de moléculas anfifílicas
CN102741430B (zh) 2009-12-01 2016-07-13 牛津楠路珀尔科技有限公司 生化分析仪器、用于进行生化分析的第一模块以及相关方法
US8828211B2 (en) 2010-06-08 2014-09-09 President And Fellows Of Harvard College Nanopore device with graphene supported artificial lipid membrane
AU2012215135B9 (en) 2011-02-11 2017-03-09 Oxford Nanopore Technologies Limited Mutant pores
EP3663412B1 (fr) 2011-09-23 2022-03-09 Oxford Nanopore Technologies PLC Analyse d'un polymère comprenant des unités polymères par translocation à travers une nanopore
JP6226869B2 (ja) 2011-10-21 2017-11-08 オックスフォード ナノポール テクノロジーズ リミテッド 酵素法
GB201120910D0 (en) 2011-12-06 2012-01-18 Cambridge Entpr Ltd Nanopore functionality control
BR112014016112B8 (pt) 2011-12-29 2022-12-27 Oxford Nanopore Tech Ltd Método e aparelho para caracterizar um polinucleotídeo alvo, kit, e, uso de uma helicase
US9617591B2 (en) 2011-12-29 2017-04-11 Oxford Nanopore Technologies Ltd. Method for characterising a polynucleotide by using a XPD helicase
BR112014020211A2 (pt) 2012-02-16 2017-07-04 Oxford Nanopore Tech Ltd métodos para analisar uma série ordenada por tempo de medições de um polímero, para estimar a presença, ausência ou quantidade de um polímero alvo, e para determinar uma alteração em um polímero, programa de computador, e, dispositivos de análise e de diagnóstico
JP6271505B2 (ja) 2012-04-10 2018-01-31 オックスフォード ナノポール テクノロジーズ リミテッド 変異体ライセニンポア
US9638744B2 (en) 2012-07-02 2017-05-02 Nxp Usa, Inc. Integrated circuit device, safety circuit, safety-critical system and method of manufacturing an integrated circuit device
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
US9797009B2 (en) 2012-07-19 2017-10-24 Oxford Nanopore Technologies Limited Enzyme construct
EP2875128B8 (fr) 2012-07-19 2020-06-24 Oxford Nanopore Technologies Limited Hélicases modifiées
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
EP2911771B1 (fr) 2012-10-26 2017-01-18 Oxford Nanopore Technologies Limited Interfaces goutelettes
ES2660671T3 (es) 2013-05-24 2018-03-23 Illumina Cambridge Limited Secuenciado pirofosforolítico usando nanoporos
CN118256603A (zh) 2013-10-18 2024-06-28 牛津纳米孔科技公开有限公司 经修饰的酶
US20170096703A1 (en) 2014-03-21 2017-04-06 Oxford Nanopore Technologies Ltd. Analysis of a polymer from multi-dimensional measurements
US10400014B2 (en) 2014-09-01 2019-09-03 Oxford Nanopore Technologies Ltd. Mutant CsgG pores
CA2967036A1 (fr) * 2014-11-13 2016-05-19 The Board Of Trustees Of The University Of Illinois "super" helicases hyper-fonctionnelle bio-modifiees
AU2017225374B2 (en) 2016-03-02 2020-03-05 Oxford Nanopore Technologies Limited Mutant pore
GB201620450D0 (en) 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
GB201707138D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Machine learning analysis of nanopore measurements
WO2019002893A1 (fr) 2017-06-30 2019-01-03 Vib Vzw Nouveaux pores protéiques
US11789006B2 (en) 2019-03-12 2023-10-17 Oxford Nanopore Technologies Plc Nanopore sensing device, components and method of operation

Also Published As

Publication number Publication date
CN121100125A (zh) 2025-12-09
WO2024235919A1 (fr) 2024-11-21
GB202307141D0 (en) 2023-06-28

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