EP4713681A2 - Procédé de photomarquage de biomolécules à l'aide de sondes photoréactives - Google Patents

Procédé de photomarquage de biomolécules à l'aide de sondes photoréactives

Info

Publication number
EP4713681A2
EP4713681A2 EP24832997.1A EP24832997A EP4713681A2 EP 4713681 A2 EP4713681 A2 EP 4713681A2 EP 24832997 A EP24832997 A EP 24832997A EP 4713681 A2 EP4713681 A2 EP 4713681A2
Authority
EP
European Patent Office
Prior art keywords
photoreactive
sample
probe
moiety
interest
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP24832997.1A
Other languages
German (de)
English (en)
Inventor
Yi-De Chen
Chih-Wei Chang
Hoi Yin CHEUNG
Chia-Wen Chung
Hsiao-Jen CHANG
Yong-Da Sie
Weng Man Chong
Jung-Chi LIAO
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Syncell Taiwan Inc
Original Assignee
Syncell Taiwan Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syncell Taiwan Inc filed Critical Syncell Taiwan Inc
Publication of EP4713681A2 publication Critical patent/EP4713681A2/fr
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2440/00—Post-translational modifications [PTMs] in chemical analysis of biological material
    • G01N2440/32—Post-translational modifications [PTMs] in chemical analysis of biological material biotinylation

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Est proposé un procédé de marquage photochimique comprenant les étapes consistant à administrer une sonde photoréactive à un échantillon, et la sonde photoréactive de formule (I) : T─L─W, la partie T étant de la biotine ou une autre fraction à base de biotine, la partie L comprenant une liaison chimique ou un agent de liaison, la partie W étant une fraction photoréactive, la fraction photoréactive pouvant absorber de la lumière ultraviolette et devenir active à des fins de réticulation avec un acide aminé ; et éclairer sélectivement l'échantillon avec un rayonnement optique pour activer la fraction photoréactive de la sonde photoréactive dans la région d'intérêt sélectionnée ; et former une réticulation non spécifique entre la fraction photoréactive de la sonde et une pluralité d'acides aminés de différentes protéines de l'échantillon dans la région d'intérêt sélectionnée.
EP24832997.1A 2023-06-29 2024-06-28 Procédé de photomarquage de biomolécules à l'aide de sondes photoréactives Pending EP4713681A2 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202363511010P 2023-06-29 2023-06-29
US202363517437P 2023-08-03 2023-08-03
PCT/US2024/036001 WO2025006872A2 (fr) 2023-06-29 2024-06-28 Procédé de photomarquage de biomolécules à l'aide de sondes photoréactives

Publications (1)

Publication Number Publication Date
EP4713681A2 true EP4713681A2 (fr) 2026-03-25

Family

ID=93940040

Family Applications (1)

Application Number Title Priority Date Filing Date
EP24832997.1A Pending EP4713681A2 (fr) 2023-06-29 2024-06-28 Procédé de photomarquage de biomolécules à l'aide de sondes photoréactives

Country Status (3)

Country Link
EP (1) EP4713681A2 (fr)
TW (1) TWI904733B (fr)
WO (1) WO2025006872A2 (fr)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020164649A1 (en) * 2000-10-25 2002-11-07 Rajendra Singh Mass tags for quantitative analysis
KR20220119066A (ko) * 2019-12-19 2022-08-26 스키노시브 접착성 광보호성 화합물 및 이의 용도
WO2021188978A1 (fr) * 2020-03-20 2021-09-23 Academia Sinica Sondes photoréactives et clivables permettant le marquage de biomolécules

Also Published As

Publication number Publication date
TWI904733B (zh) 2025-11-11
WO2025006872A2 (fr) 2025-01-02
TW202500977A (zh) 2025-01-01
WO2025006872A3 (fr) 2025-05-08

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