EP4716748A2 - Méthode de modulation de traduction d'arnm - Google Patents

Méthode de modulation de traduction d'arnm

Info

Publication number
EP4716748A2
EP4716748A2 EP24811894.5A EP24811894A EP4716748A2 EP 4716748 A2 EP4716748 A2 EP 4716748A2 EP 24811894 A EP24811894 A EP 24811894A EP 4716748 A2 EP4716748 A2 EP 4716748A2
Authority
EP
European Patent Office
Prior art keywords
translational
utr
mrna molecule
mrna
modified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP24811894.5A
Other languages
German (de)
English (en)
Inventor
Wendy GILBERT
Carson THOREEN
Cole LEWIS
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yale University
Original Assignee
Yale University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yale University filed Critical Yale University
Publication of EP4716748A2 publication Critical patent/EP4716748A2/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des méthodes de modulation des efficacités de traduction d'ARNm avec des activateurs ou des ralentisseurs de la traduction qui affectent la rétention des ribosomes. L'invention concerne également des molécules d'ARNm comprenant des activateurs ou des ralentisseurs de la traduction, ainsi que des nucléobases modifiées. L'invention concerne en outre des méthodes de construction d'une molécule d'ARNm pour produire un peptide thérapeutique ou une protéine thérapeutique avec les activateurs identifiés.
EP24811894.5A 2023-05-23 2024-05-23 Méthode de modulation de traduction d'arnm Pending EP4716748A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363503837P 2023-05-23 2023-05-23
PCT/US2024/030724 WO2024243389A2 (fr) 2023-05-23 2024-05-23 Méthode de modulation de traduction d'arnm

Publications (1)

Publication Number Publication Date
EP4716748A2 true EP4716748A2 (fr) 2026-04-01

Family

ID=93590391

Family Applications (1)

Application Number Title Priority Date Filing Date
EP24811894.5A Pending EP4716748A2 (fr) 2023-05-23 2024-05-23 Méthode de modulation de traduction d'arnm

Country Status (4)

Country Link
EP (1) EP4716748A2 (fr)
CN (1) CN121532515A (fr)
AU (1) AU2024274968A1 (fr)
WO (1) WO2024243389A2 (fr)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2990881C (fr) * 2015-06-30 2024-02-20 Ethris Gmbh Regions non traduites (utr) augmentant l'efficacite de traduction des molecules d'arn
WO2023172896A2 (fr) * 2022-03-07 2023-09-14 Yale University Méthode de modulation de traduction d'arnm

Also Published As

Publication number Publication date
WO2024243389A3 (fr) 2025-04-03
AU2024274968A1 (en) 2026-01-22
WO2024243389A2 (fr) 2024-11-28
CN121532515A (zh) 2026-02-13

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