EP4724558A1 - Modèle de peau bio-imprimée, procédé d'impression tridimensionnelle associé et dispositif de perfusion directe équipé dudit modèle - Google Patents

Modèle de peau bio-imprimée, procédé d'impression tridimensionnelle associé et dispositif de perfusion directe équipé dudit modèle

Info

Publication number
EP4724558A1
EP4724558A1 EP24737819.3A EP24737819A EP4724558A1 EP 4724558 A1 EP4724558 A1 EP 4724558A1 EP 24737819 A EP24737819 A EP 24737819A EP 4724558 A1 EP4724558 A1 EP 4724558A1
Authority
EP
European Patent Office
Prior art keywords
fact
skin
fibroblasts
model
endothelial cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP24737819.3A
Other languages
German (de)
English (en)
Inventor
Lorenzo Maria VISENTIN
Federico MAGGIOTTO
Elisa CIMETTA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio System Lab Srl
Original Assignee
Bio System Lab Srl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio System Lab Srl filed Critical Bio System Lab Srl
Publication of EP4724558A1 publication Critical patent/EP4724558A1/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/08—Chemical, biochemical or biological means, e.g. plasma jet, co-culture

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physiology (AREA)
  • Cell Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Le modèle de peau bio-imprimée (1) comporte : - au moins une couche dermique (2) comportant au moins une matrice polymère et un mélange de fibroblastes et de cellules endothéliales et conçue pour simuler un tissu dermique humain ; - au moins une couche épidermique (3) comportant au moins un mélange de kératinocytes et conçue pour simuler un tissu épidermique humain ; la couche dermique (2) comportant au moins un conduit interne (4) constitué de fibroblastes et de cellules endothéliales, conçu pour simuler un canal vasculaire de la peau humaine et pourvu d'au moins deux tronçons d'extrémité communiquant avec l'extérieur et pouvant être reliés à un système de perfusion active de substances.
EP24737819.3A 2023-06-06 2024-06-05 Modèle de peau bio-imprimée, procédé d'impression tridimensionnelle associé et dispositif de perfusion directe équipé dudit modèle Pending EP4724558A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IT102023000011436A IT202300011436A1 (it) 2023-06-06 2023-06-06 Modello di pelle biostampato, relativo processo di stampa tridimensionale e dispositivo di perfusione diretta provvisto di tale modello
PCT/IB2024/055478 WO2024252292A1 (fr) 2023-06-06 2024-06-05 Modèle de peau bio-imprimée, procédé d'impression tridimensionnelle associé et dispositif de perfusion directe équipé dudit modèle

Publications (1)

Publication Number Publication Date
EP4724558A1 true EP4724558A1 (fr) 2026-04-15

Family

ID=87889617

Family Applications (1)

Application Number Title Priority Date Filing Date
EP24737819.3A Pending EP4724558A1 (fr) 2023-06-06 2024-06-05 Modèle de peau bio-imprimée, procédé d'impression tridimensionnelle associé et dispositif de perfusion directe équipé dudit modèle

Country Status (4)

Country Link
EP (1) EP4724558A1 (fr)
CN (1) CN121693559A (fr)
IT (1) IT202300011436A1 (fr)
WO (1) WO2024252292A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119823871A (zh) * 2025-03-19 2025-04-15 安徽骆华生物科技有限公司 血管化皮肤芯片、血管化皮肤模型及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6619345B2 (ja) * 2014-09-23 2019-12-11 国立大学法人 東京大学 人工三次元組織の製造方法、人工三次元組織灌流デバイス、人工三次元組織を用いた薬剤評価方法
JP6917975B2 (ja) * 2015-07-06 2021-08-11 アドバンスト・ソリューションズ・ライフ・サイエンシズ，エルエルシー 体外血管かん流装置、医薬品スクリーニング装置、及び体外微小循環システムの製造方法
WO2018049097A1 (fr) * 2016-09-09 2018-03-15 The Government Of The United States Of America, As Represented By The Secretary Of The Navy Modèles de peau vascularisée
WO2018165613A1 (fr) * 2017-03-10 2018-09-13 Prellis Biologics, Inc. Procédés et systèmes d'impression d'un matériau biologique
US20190216984A1 (en) * 2018-07-26 2019-07-18 The Fourth Military Medical University Vascularized full thickness tissue-engineered skin assembled by hydrogel, nanofibrous scaffolds and skin cell layers and preparation method thereof

Also Published As

Publication number Publication date
IT202300011436A1 (it) 2024-12-06
WO2024252292A1 (fr) 2024-12-12
CN121693559A (zh) 2026-03-17

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