EP4728061A1 - Verfahren zum screening eines moleküls zur bindung an ein protein von interesse - Google Patents

Verfahren zum screening eines moleküls zur bindung an ein protein von interesse

Info

Publication number
EP4728061A1
EP4728061A1 EP24731036.0A EP24731036A EP4728061A1 EP 4728061 A1 EP4728061 A1 EP 4728061A1 EP 24731036 A EP24731036 A EP 24731036A EP 4728061 A1 EP4728061 A1 EP 4728061A1
Authority
EP
European Patent Office
Prior art keywords
fragment
fusion protein
protein
nucleic acid
poi
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP24731036.0A
Other languages
English (en)
French (fr)
Inventor
Dennis Gillingham
Lukas Schneider
Basilius SAUTER
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universitaet Basel
Original Assignee
Universitaet Basel
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universitaet Basel filed Critical Universitaet Basel
Publication of EP4728061A1 publication Critical patent/EP4728061A1/de
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1264—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal nucleotidyl transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07031—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal deoxynucleotidyl transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01001—Carbonate dehydratase (4.2.1.1), i.e. carbonic anhydrase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
EP24731036.0A 2023-06-13 2024-06-11 Verfahren zum screening eines moleküls zur bindung an ein protein von interesse Pending EP4728061A1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP23178853 2023-06-13
EP23206019 2023-10-26
PCT/EP2024/066039 WO2024256374A1 (en) 2023-06-13 2024-06-11 Method for screening a molecule for binding to a protein of interest

Publications (1)

Publication Number Publication Date
EP4728061A1 true EP4728061A1 (de) 2026-04-22

Family

ID=91376951

Family Applications (1)

Application Number Title Priority Date Filing Date
EP24731036.0A Pending EP4728061A1 (de) 2023-06-13 2024-06-11 Verfahren zum screening eines moleküls zur bindung an ein protein von interesse

Country Status (3)

Country Link
EP (1) EP4728061A1 (de)
CN (1) CN121816410A (de)
WO (1) WO2024256374A1 (de)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10215035A1 (de) * 2002-04-05 2003-10-23 Roche Diagnostics Gmbh Rekombinante terminale Deoxynukleotidyltransferase mit verbesserter Funktionalität
AU2017280335B2 (en) * 2016-06-24 2023-06-29 The Regents Of The University Of California Nucleic acid synthesis and sequencing using tethered nucleoside triphosphates
EP4150065A2 (de) * 2020-05-12 2023-03-22 Illumina Inc Erzeugung von nukleinsäuren mit modifizierten basen unter verwendung rekombinanter terminaler deoxynucleotidyltransferase
WO2023064923A2 (en) * 2021-10-15 2023-04-20 Mammoth Biosciences, Inc. Fusion effector proteins and uses thereof
WO2023086767A1 (en) * 2021-11-12 2023-05-19 Leash Labs, Inc. High-throughput drug discovery methods

Also Published As

Publication number Publication date
CN121816410A (zh) 2026-04-07
WO2024256374A1 (en) 2024-12-19

Similar Documents

Publication Publication Date Title
KR102839867B1 (ko) Fgfr 억제제를 사용한 치료에 반응할 암 환자를 확인하는 데 있어서 fgfr 돌연변이 유전자 패널의 사용
KR102031715B1 (ko) 표적 단백질에 결합하는 핵산 단편
US9517252B2 (en) p53 activating peptides
US10590480B2 (en) Polymerase variants
US11807848B2 (en) High throughput discovery of new genes from complex mixtures of environmental microbes
US10240147B2 (en) Production of encoded chemical libraries
JP2018531002A (ja) Ｏｍｐｇバリアント
JP2018531002A6 (ja) Ｏｍｐｇバリアント
CN104611422A (zh) 一种检测大肠杆菌耐氟喹诺酮类gyrA、parC基因点突变方法
EP3802875A1 (de) Enzymatische anreicherung von dna-poren-polymerase-komplexen
Yirmiya et al. Systematic discovery of TIR-based immune signaling systems in bacteria
Homiski et al. The mutant βE202K sliding clamp protein impairs DNA polymerase III replication activity
WO2024256374A1 (en) Method for screening a molecule for binding to a protein of interest
KR101661507B1 (ko) 미코박테리움 튜버쿨로시스 억제용 단일가닥 핵산 앱타머
JP2017506912A (ja) 蛍光ベースの検出方法によるサンプル中の微生物を検出する方法
RU2737829C1 (ru) Последовательность ДНК-аптамера, связывающаяся с пептидогликан-ассоциированным липопротеином Legionella pneumophila
WO2015131302A1 (zh) 一种具有持续合成能力和盐耐受度的dna聚合酶
Martínez-García et al. Differential expression of the apsXRS system by antimicrobial peptide LL-37 in commensal and clinical Staphylococcus epidermidis isolates
KR20260005347A (ko) 조작된 tet 효소 및 후성유전학 및 차세대 서열분석(ngs), 예컨대 tet-보조 피리딘 보란 서열분석(taps)에서의 용도
KR101347917B1 (ko) 탄저균 독소의 방어항원에 특이적인 앱타머를 선별하는 방법
KR20220012896A (ko) 배설물 시료 내 희귀 dna 서열의 검출 방법
US20060223084A1 (en) Methods and compositions for detecting Bacillus anthracis
JP2003061665A (ja) 芽胞形成菌の検出方法
JP4651666B2 (ja) 単一の反応槽において処理される生体試料中に存在する、標的の核酸の標識及び精製方法
Antar Investigating the role of CTP binding and hydrolysis in mediating the functions of ParB and ParB-like proteins

Legal Events

Date Code Title Description
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: UNKNOWN

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE

PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE

17P Request for examination filed

Effective date: 20260109

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC ME MK MT NL NO PL PT RO RS SE SI SK SM TR