ES2000404A6 - Un metodo de transformacion del actinomiceto streptomyces wadayamensis - Google Patents

Un metodo de transformacion del actinomiceto streptomyces wadayamensis

Info

Publication number
ES2000404A6
ES2000404A6 ES8602191A ES8602191A ES2000404A6 ES 2000404 A6 ES2000404 A6 ES 2000404A6 ES 8602191 A ES8602191 A ES 8602191A ES 8602191 A ES8602191 A ES 8602191A ES 2000404 A6 ES2000404 A6 ES 2000404A6
Authority
ES
Spain
Prior art keywords
protoplasts
transformation method
wadayamensis
actinomycete streptomyces
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES8602191A
Other languages
English (en)
Inventor
Sarabia Cristina Acebal
Lopez De La Isidra Gab Marquez
Susan Victor Rubio
Ortega Agustin Perez-Aranda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Antibioticos SA
Original Assignee
Antibioticos SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Antibioticos SA filed Critical Antibioticos SA
Publication of ES2000404A6 publication Critical patent/ES2000404A6/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

LA PRESENTE INVENCION SE REFIERE A UN METOD PARA LA TRANSFORMACION DEL ACTINOMICETO S. WADAYAMENSIS. EXISTEN DIFERENTES PROCESOS PARA CAMBIAR LA INFORMACION GENETICA DE UN ORGANISMO. GENERALMENTE, LA TRANSFORMACION COMPRENDE LA INTRODUCCION DE UN DNA PLASMIDICO EN UNA CELULA EN PRESENCIA DE IONES CA++ Y POLIETILENGLICOL. LA METODOLOGIA DE LA PRESENTE INVENCION COMPRENDE LA FORMACION Y EL AISLAMIENTO DE PROTOPLASTOS DE S. WADAYAMENSIS, LA INCUBACION DE LOS PROTOPLASTOS CON DNA PLASMIDICO EN PRESENCIA DE POLIETILENGLICOL Y CA++ PARA INTRODUCIR EL DNA EN LOS PROTOPLASTOS; EL CULTIVO DE LOS PROTOPLASTOS EN UN MEDIO ADECUADO PARA REGENERARLOS A CELULAS NORMALES Y LA RECUPERACION DE UNA CEPA CON EL FENOTIPO DERIVADO DEL DNA PLASMIDICO INTRODUCIDO.
ES8602191A 1985-09-26 1986-09-25 Un metodo de transformacion del actinomiceto streptomyces wadayamensis Expired ES2000404A6 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB858523768A GB8523768D0 (en) 1985-09-26 1985-09-26 Streptomyces wadayamensis

Publications (1)

Publication Number Publication Date
ES2000404A6 true ES2000404A6 (es) 1988-02-16

Family

ID=10585765

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8602191A Expired ES2000404A6 (es) 1985-09-26 1986-09-25 Un metodo de transformacion del actinomiceto streptomyces wadayamensis

Country Status (2)

Country Link
ES (1) ES2000404A6 (es)
GB (2) GB8523768D0 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8630527D0 (en) * 1986-12-22 1987-02-04 Sandoz Ltd Organic compounds
EP0368224A3 (de) * 1988-11-10 1991-12-27 Hoechst Aktiengesellschaft Lysozymgen aus Streptomyceten, Verfahren zu seiner Gewinnung und seine Verwendung

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0006692A1 (en) * 1978-06-01 1980-01-09 National Research Development Corporation Streptomyces plasmids and microorganisms containing them, their use as vectors for nucleic acid and a process for the incorporation of nucleic acids into cellular systems
EP0118367B1 (en) * 1983-03-08 1989-04-26 Merck & Co. Inc. Recombinant dna cloning vector pve1, deletion and hybrid mutants, and recombinant derivatives thereof, products and processes
GB8333544D0 (en) * 1983-12-16 1984-01-25 Pfizer Ltd Streptomycete host cells

Also Published As

Publication number Publication date
GB8623102D0 (en) 1986-10-29
GB2182673A (en) 1987-05-20
GB8523768D0 (en) 1985-10-30
GB2182673B (en) 1990-03-21

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Legal Events

Date Code Title Description
SA6 Expiration date (snapshot 920101)

Free format text: 2006-09-25