ES2003085A6 - Procedimiento para inducir de una forma controlada la expresion de genes eucarioticos de interes en celulas hospedantes eucarioticas apropiadas - Google Patents

Procedimiento para inducir de una forma controlada la expresion de genes eucarioticos de interes en celulas hospedantes eucarioticas apropiadas

Info

Publication number
ES2003085A6
ES2003085A6 ES8600725A ES8600725A ES2003085A6 ES 2003085 A6 ES2003085 A6 ES 2003085A6 ES 8600725 A ES8600725 A ES 8600725A ES 8600725 A ES8600725 A ES 8600725A ES 2003085 A6 ES2003085 A6 ES 2003085A6
Authority
ES
Spain
Prior art keywords
heat
shock
promoter
production
promoters
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES8600725A
Other languages
English (en)
Inventor
Peter Bromley
Michel Dreano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Battelle Memorial Institute Inc
Original Assignee
Battelle Memorial Institute Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Battelle Memorial Institute Inc filed Critical Battelle Memorial Institute Inc
Publication of ES2003085A6 publication Critical patent/ES2003085A6/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Endocrinology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

PROCEDIMIENTO PARA INDUCIR DE UNA FORMA CONTROLADA LA EXPRESION DE GENES EUCARIOTICOS DE INTERES EN CELULAS HOSPEDANTES EUCARIOTICAS APROPIADAS, CARACTERIZADO PORQUE COMPRENDE LAS FASES DE PROPORCIONAR UNA UNIDAD DE EXPRESION DE GEN DE DNA RECOMBINANTE EN LA CUAL DICHO GEN DE INTERES SE ENLAZA FUNCIONALMENTE BAJO CONTROL DE TRANSCRIPCION Y/O DE TRASLACION DE POR LO MENOS UN ELEMENTO DE CONTROL DE CHOQUE TERMICO DE ORIGEN EUCARIOTICO Y QUE INCLUYE UNA O MAS SECUENCIAS SIN TRASLADAR 5K Y/O 3K AL REFERIDO GEN DE INTERES; INTRODUCIR UNA O MAS VARIACIONES O MODIFICACIONES DE LAS SECUENCIAS EN LAS REFERIDAS SECUENCIAS NO TRASLADADAS PARA MEJORAR LA EFICACIA DE LA EXPRESION DESPUES DE LA INDUCCION; INTRODUCIR DICHA UNIDAD DE EXPRESION DE GEN MODIFICADA EN CELULAS HOSPEDANTES IDONEAS DE ORIGEN EUCARIOTICO Y SOMETER EL CULTIVO A TENSION, POR LO QUE SE INDUCE LA EXPRESION DE LOS GENES EN PRODUCTOS DE INTERES.
ES8600725A 1985-07-31 1986-07-30 Procedimiento para inducir de una forma controlada la expresion de genes eucarioticos de interes en celulas hospedantes eucarioticas apropiadas Expired ES2003085A6 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP85810354 1985-07-31

Publications (1)

Publication Number Publication Date
ES2003085A6 true ES2003085A6 (es) 1988-10-16

Family

ID=8194647

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8600725A Expired ES2003085A6 (es) 1985-07-31 1986-07-30 Procedimiento para inducir de una forma controlada la expresion de genes eucarioticos de interes en celulas hospedantes eucarioticas apropiadas

Country Status (5)

Country Link
EP (1) EP0231368A1 (es)
AU (1) AU604214B2 (es)
ES (1) ES2003085A6 (es)
WO (1) WO1987000861A1 (es)
ZA (1) ZA865702B (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU606049B2 (en) * 1986-02-06 1991-01-31 General Hospital Corporation, The Inducible heat shock and amplification system
WO1988001645A1 (en) * 1986-08-26 1988-03-10 Macquarie University Transformation of an organism using antisense rna or promoter occlusion technique
US5521084A (en) * 1992-11-10 1996-05-28 Biostar, Inc. Bovine heat shock promoter and uses thereof
EP0922110B1 (en) 1996-08-15 2008-04-16 The Government of the United States of America, represented by the Secretary, Department of Health and Human Services Spatial and temporal control of gene expression using a heat shock protein promoter in combination with local heat
US7279565B2 (en) * 1998-05-05 2007-10-09 Richard Voellmy Molecular regulatory circuits to achieve sustained activation of genes of interest by a single stress
GB9905498D0 (en) 1999-03-11 1999-05-05 Glaxo Group Ltd Expression
AT500838B1 (de) * 2004-04-20 2007-11-15 Veterinaermedizinische Uni Wie Multiple hse
ITUB20153898A1 (it) * 2015-09-25 2017-03-25 Univ Degli Studi Di Palermo Etichette intelligenti per la rilevazione visiva del deterioramento dei prodotti alimentari

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO172595C (no) * 1983-02-07 1993-08-11 Battelle Memorial Institute Fremgangsmaate og ekspresjonsplasmid for fremstilling av hemagglutinin under kontroll av drosophila hsp-promoter
US5447858A (en) * 1984-04-13 1995-09-05 Mycogen Plant Sciences, Inc. Heat shock promoter and gene
CA1272142A (en) * 1984-06-11 1990-07-31 Cetus Corporation Mammalian promoters useful in yeast expression

Also Published As

Publication number Publication date
ZA865702B (en) 1987-03-25
AU6286086A (en) 1987-03-05
WO1987000861A1 (en) 1987-02-12
AU604214B2 (en) 1990-12-13
EP0231368A1 (en) 1987-08-12

Similar Documents

Publication Publication Date Title
SE8603145D0 (sv) Forfarande for framstellning av interferon-beta
DE3880468D1 (de) Rekombinante dns-expressionsvektoren.
Climie et al. Chemical synthesis of the thymidylate synthase gene.
ES8401777A1 (es) Procedimiento para producir un polipeptido.
KR880014110A (ko) 인체 프로아포지방단백질 a-i의 발현
NO173741C (no) DNA-sekvens for modifisert protein C, vektor inneholdende denne, patte-dyrceller transfektert med vektorer, og fremgangsmaate for fremstilling av nevnte protein
HUT72987A (en) Adenovirus vectors for the transfer of foreign genes into cells of the central nervous system, particularly in brain
Frey et al. Replication and copy number control of the broad-host-range plasmid RSF1010
BR1100252A (pt) Gene quimérico
IL91826A (en) Human growth hormone analog
MX9602993A (es) Hormona del crecimiento humano.
DE3688900D1 (de) Expression von protein c.
Pečenková et al. Bacteriophage B103: complete DNA sequence of its genome and relationship to other Bacillus phages
HUT68379A (en) Method for modifying the cell, tissue or host tropism of a recombinant microorganism obtained this way and use thereof in medicine and veterinary medicine
ZA869487B (en) Process for the improvement of the expression of eukaryont proteins
FI841976A7 (fi) Plasmider med villkorligt okontrollerat replikeringsuppfoerande.
Hoffmann et al. Overproduction and large‐scale preparation of deoxyuridine triphosphate nucleotidohydrolase from Escherichia coli
DK587488A (da) Fibroblast-vaekstfaktor, nucleotidsekvens kodende derfor, praeparat indeholdende faktoren og fremgangsmaade til fremstilling deraf
Price et al. Regulation of lipoprotein lipase synthesis in 3T3-L1 adipocytes by cachectin Further proof for identity with tumour necrosis factor
GB2171703B (en) Expression-secretion vector for gene expression and protein secretion, recombinant dna including the vector, and method of producing proteins by use of the re
EP0464832A3 (en) Cloned n-methylhydantoinase
EP0164245A3 (en) Nif promoter of rhizobium japonicum
KR910001046A (ko) 비융합 단백질을 제조하기 위한 미생물내 발현용 발현벡터
JPS5756495A (en) Novel dna-introduction vector and recombinant dna
ES2181684T3 (es) Promotor del gen epidermico humano.

Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19980601