ES2003262A6 - Process for extracting plasminogen tissue activator from pregnant sow ovary - Google Patents

Process for extracting plasminogen tissue activator from pregnant sow ovary

Info

Publication number
ES2003262A6
ES2003262A6 ES8701138A ES8701138A ES2003262A6 ES 2003262 A6 ES2003262 A6 ES 2003262A6 ES 8701138 A ES8701138 A ES 8701138A ES 8701138 A ES8701138 A ES 8701138A ES 2003262 A6 ES2003262 A6 ES 2003262A6
Authority
ES
Spain
Prior art keywords
phase
extracting
pregnant sow
tissue activator
plasminogen tissue
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES8701138A
Other languages
Spanish (es)
Inventor
Pajarin Hector Alvarez
Santillan Julio Gregori Medina
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ARCHE Srl LAB
Original Assignee
ARCHE Srl LAB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ARCHE Srl LAB filed Critical ARCHE Srl LAB
Priority to ES8701138A priority Critical patent/ES2003262A6/en
Priority to FR8706921A priority patent/FR2614037A1/en
Priority to IT8748015A priority patent/IT1227776B/en
Publication of ES2003262A6 publication Critical patent/ES2003262A6/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Process for extracting plasminogen tissue activator from pregnant sow ovary. The ovaries, separated from the fallopian tubes, are carefully cleaned and ground until they are converted into a paste, then subjected to a lipid-removal phase, followed by a phase for extraction with potassium thiocyanate (KSCN). After another resuspension phase with potassium thiocyanate, they are subjected to an acidification phase, then to a dilution phase, next to another extraction phase with fibrin and, finally, to a phase for separating the tPA which is completed by a terminal purification phase.
ES8701138A 1987-04-20 1987-04-20 Process for extracting plasminogen tissue activator from pregnant sow ovary Expired ES2003262A6 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
ES8701138A ES2003262A6 (en) 1987-04-20 1987-04-20 Process for extracting plasminogen tissue activator from pregnant sow ovary
FR8706921A FR2614037A1 (en) 1987-04-20 1987-05-18 Process for extracting plasminogen tissue activator from pregnant sow ovary
IT8748015A IT1227776B (en) 1987-04-20 1987-06-03 PROCEDURE FOR EXTRACTION OF THE TISSULAR ACTIVATOR OF THE PLASMINOGEN STARTING FROM THE OVERSE OF SEVERE SCROPHES

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES8701138A ES2003262A6 (en) 1987-04-20 1987-04-20 Process for extracting plasminogen tissue activator from pregnant sow ovary

Publications (1)

Publication Number Publication Date
ES2003262A6 true ES2003262A6 (en) 1988-10-16

Family

ID=8250520

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8701138A Expired ES2003262A6 (en) 1987-04-20 1987-04-20 Process for extracting plasminogen tissue activator from pregnant sow ovary

Country Status (3)

Country Link
ES (1) ES2003262A6 (en)
FR (1) FR2614037A1 (en)
IT (1) IT1227776B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0307774A3 (en) * 1987-09-16 1990-02-28 W.R. Grace & Co.-Conn. Process for purifying plasminogen activator

Also Published As

Publication number Publication date
IT8748015A0 (en) 1987-06-03
FR2614037A1 (en) 1988-10-21
IT1227776B (en) 1991-05-07

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20000401