ES2005756A6 - Un metodo para liberar polipeptidos heterologos de proteinas de fusion. - Google Patents

Un metodo para liberar polipeptidos heterologos de proteinas de fusion.

Info

Publication number
ES2005756A6
ES2005756A6 ES8800090A ES8800090A ES2005756A6 ES 2005756 A6 ES2005756 A6 ES 2005756A6 ES 8800090 A ES8800090 A ES 8800090A ES 8800090 A ES8800090 A ES 8800090A ES 2005756 A6 ES2005756 A6 ES 2005756A6
Authority
ES
Spain
Prior art keywords
fusion proteins
producing fusion
optionally
linker
coli
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES8800090A
Other languages
English (en)
Inventor
Hardy W Chan
Earl R Shelton
Preston A Baecker
Felix H Salazar
Paula R Szoka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Syntex USA LLC
Original Assignee
Syntex USA LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syntex USA LLC filed Critical Syntex USA LLC
Publication of ES2005756A6 publication Critical patent/ES2005756A6/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/60—Growth hormone-releasing factor [GH-RF], i.e. somatoliberin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
    • C12N2740/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16211—Human Immunodeficiency Virus, HIV concerning HIV gagpol
    • C12N2740/16222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Endocrinology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

EL INVENTO CONCIERNE A UN METODO PARA LIBERAR POLIPEPTIDOS HETEROLOGOS DE PROTEINAS DE FUSION QUE TIENEN UN ENLACE ASP-PRO Y UN POLIPEPTIDO HETEROLOGO, COMPRENDIENDO DICHO METODO: HIDROLIZAR EL ENLACE ASP-PRO EN CONDICIONES ACIDAS, PREFERIBLEMENTE A PH 2-4.
ES8800090A 1985-08-12 1988-01-15 Un metodo para liberar polipeptidos heterologos de proteinas de fusion. Expired ES2005756A6 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US76507385A 1985-08-12 1985-08-12
US76507285A 1985-08-12 1985-08-12

Publications (1)

Publication Number Publication Date
ES2005756A6 true ES2005756A6 (es) 1989-03-16

Family

ID=27117556

Family Applications (3)

Application Number Title Priority Date Filing Date
ES8601007A Expired ES2000858A6 (es) 1985-08-12 1986-08-11 Un metodo para producir un vector de expresion bacteriano para la expresion de proteinas de fusion
ES8800090A Expired ES2005756A6 (es) 1985-08-12 1988-01-15 Un metodo para liberar polipeptidos heterologos de proteinas de fusion.
ES8800091A Expired ES2005757A6 (es) 1985-08-12 1988-01-15 Un metodo para producir proteinas de fusion por medios recombinantes.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES8601007A Expired ES2000858A6 (es) 1985-08-12 1986-08-11 Un metodo para producir un vector de expresion bacteriano para la expresion de proteinas de fusion

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES8800091A Expired ES2005757A6 (es) 1985-08-12 1988-01-15 Un metodo para producir proteinas de fusion por medios recombinantes.

Country Status (3)

Country Link
EP (1) EP0213472A1 (es)
AU (1) AU6104786A (es)
ES (3) ES2000858A6 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8412517D0 (en) * 1984-05-16 1984-06-20 Nagai K Recombinant fusion proteins
DE3727137A1 (de) * 1987-08-14 1989-02-23 Behringwerke Ag Herstellung von antigenen des human immunodeficiency virus typ 1 sowie deren verwendung als diagnostisches mittel und therapeutikum
NZ242344A (en) * 1991-04-19 1993-11-25 Lilly Co Eli Dna encoding bovine growth hormone releasing factor and precursors, the polypeptide precursor and pharmaceutical compositions
US6242219B1 (en) * 1999-03-18 2001-06-05 Xoma (Us) Llc Methods for recombinant peptide production
EP2290088A1 (en) 2004-12-22 2011-03-02 Genentech, Inc. Methods for producing soluble multi-menbrane-spanning proteins
US8609621B2 (en) 2010-11-15 2013-12-17 E I Du Pont De Nemours And Company Acid-cleavable linkers exhibiting altered rates of acid hydrolysis
CN116254283B (zh) * 2021-12-02 2026-07-28 中国科学院分子细胞科学卓越创新中心 一种单次跨膜蛋白的跨膜域的制备方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA811368B (en) * 1980-03-24 1982-04-28 Genentech Inc Bacterial polypedtide expression employing tryptophan promoter-operator
US4738921A (en) * 1984-09-27 1988-04-19 Eli Lilly And Company Derivative of the tryptophan operon for expression of fused gene products

Also Published As

Publication number Publication date
ES2000858A6 (es) 1988-03-16
EP0213472A1 (en) 1987-03-11
AU6104786A (en) 1987-02-19
ES2005757A6 (es) 1989-03-16

Similar Documents

Publication Publication Date Title
AU573523B2 (en) Functional human urokinase proteins
DE3664483D1 (en) Method for the microbiological synthesis of human serum albumin
AU2013688A (en) Dna constructs containing a kluyveromyces alpha-factor leader sequence for directing secretion of heterologous polypeptides
AU6733487A (en) Mammalian interleukin-4
CA2206399A1 (en) Method for secreting thrombopoietin polypeptides
AU4491685A (en) Hybrid protein and fused gene encoding gene
ATA192895A (de) Herstellung von proteinen aus pro-proteinen durch fusionsproteine abgeleitet von furin oder furinanalogen
IL80562A0 (en) Recombinant human lysozyme,its preparation by recombinant dna technology and pharmaceutical compositions containing it
MY103362A (en) Production of proteins in active forms
AU4754490A (en) Heat-labile toxin b subunit fusion proteins
AU5882486A (en) Releasing desired peptides from fusion polypeptides using endopeptidases
DK0453969T3 (es)
AU4927390A (en) Fusion proteins
AU2569588A (en) A method for the selective cleavage of fusion proteins
AU1704988A (en) Peptide production by protein engineering
AU5695086A (en) A dna sequence
AU6104786A (en) Fusion proteins using trple gene
EP0343132A3 (en) Methods and systems for producing hiv antigens
ZA899338B (en) Heat-labile toxin b subunit fusion proteins