ES2008956A6 - Un procedimiebto para obtener una linea de celulas hibridas capaces deproducir anticuerpos monoclonales singularmente especificos de los neutrofilos humanos. - Google Patents

Un procedimiebto para obtener una linea de celulas hibridas capaces deproducir anticuerpos monoclonales singularmente especificos de los neutrofilos humanos.

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Publication number
ES2008956A6
ES2008956A6 ES8800066A ES8800066A ES2008956A6 ES 2008956 A6 ES2008956 A6 ES 2008956A6 ES 8800066 A ES8800066 A ES 8800066A ES 8800066 A ES8800066 A ES 8800066A ES 2008956 A6 ES2008956 A6 ES 2008956A6
Authority
ES
Spain
Prior art keywords
monoclonal antibody
produced
cell line
derived
antibody specific
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES8800066A
Other languages
English (en)
Inventor
James D Griffin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Coulter Corp
Original Assignee
Coulter Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/070,202 external-priority patent/US4931395A/en
Application filed by Coulter Corp filed Critical Coulter Corp
Publication of ES2008956A6 publication Critical patent/ES2008956A6/es
Expired legal-status Critical Current

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Abstract

UN PROCEDIMIENTO PARA OBTENER UNA LINEA DE CELULAS HIBRIDAS CAPACES DE PRODUCIR ANTICUERPOS MONOCLONALES SINGULARMENTE ESPECIFICOS DE LOS NEUTROFILOS HUMANOS, QUE COMPRENDE INMUNIZAR UN ANIMAL CON GRANULOCITOS HUMANOS, FUSIONAR CELULAS PRODUCTORAS DE ANTICUERPO DE ANIMAL INMUNIZADO CON CELULAS DE UNA LINEA CELULAR INMORTAL, CULTIVARLAS EN UN MEDIO SELECTIVO Y SELECCIONAR LAS QUE PRODUCEN EL ANTICUERPO MONOCLONAL QUE SE UNE ESPECIFICAMENTE AL ANTIGENO 103 DE NEUTROFILOS. SE DESCRIBE LA OBTENCION DE LOS CITADOS ANTICUERPOS POR CULTIVO DE LOS HIBRIDOMAS OBTENIDOS Y SEPARACION DEL ANTICUERPO. LA LINEA CELULAR HA SIDO DEPOSITADA EN LA ATCC CON EL N.G HB 9445. EL ANTICUERPO MONOCLONAL OBTENIDO NO TIENE NINGUNA REACTIVIDAD CON OTRAS CELULAS DE LA SANGRE PERIFERICA HUMANA Y PRACTICAMENTE NINGUNA REACTIVIDAD CON LOS PRECURSORES GRANULOCITICOS U OTRAS CELULAS DE LA MEDULA OSEA, NI CON CELULAS DE LA LEUCEMIA HUMANA AGUDA Y PUEDE SER UTILIZADO PARA ENUMERAR Y AISLAR NEUTROFILOS EN LA SANGRE.
ES8800066A 1987-07-06 1988-01-12 Un procedimiebto para obtener una linea de celulas hibridas capaces deproducir anticuerpos monoclonales singularmente especificos de los neutrofilos humanos. Expired ES2008956A6 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/070,202 US4931395A (en) 1986-12-08 1987-07-06 Monoclonal antibody specific to neutrophils

Publications (1)

Publication Number Publication Date
ES2008956A6 true ES2008956A6 (es) 1989-08-16

Family

ID=22093802

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8800066A Expired ES2008956A6 (es) 1987-07-06 1988-01-12 Un procedimiebto para obtener una linea de celulas hibridas capaces deproducir anticuerpos monoclonales singularmente especificos de los neutrofilos humanos.

Country Status (1)

Country Link
ES (1) ES2008956A6 (es)

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Legal Events

Date Code Title Description
SA6 Expiration date (snapshot 920101)

Free format text: 2008-01-12

FD1A Patent lapsed

Effective date: 20020401