ES2035067T3 - Un sistema de fase solida que incorpora sales de tetrazolio para uso en ensayos de ligando-receptor. - Google Patents

Un sistema de fase solida que incorpora sales de tetrazolio para uso en ensayos de ligando-receptor.

Info

Publication number
ES2035067T3
ES2035067T3 ES198787306087T ES87306087T ES2035067T3 ES 2035067 T3 ES2035067 T3 ES 2035067T3 ES 198787306087 T ES198787306087 T ES 198787306087T ES 87306087 T ES87306087 T ES 87306087T ES 2035067 T3 ES2035067 T3 ES 2035067T3
Authority
ES
Spain
Prior art keywords
phase system
solid phase
tetrazolium salt
receptor
solid support
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES198787306087T
Other languages
English (en)
Inventor
Richard R. Anderson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hybritech Inc
Original Assignee
Hybritech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hybritech Inc filed Critical Hybritech Inc
Application granted granted Critical
Publication of ES2035067T3 publication Critical patent/ES2035067T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/549—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic with antigen or antibody entrapped within the carrier

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Food Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

UN SISTEMA DE GASE SOLIDA QUE INCORPORA SALES DE TETRAZOLIUN PARA SU USO EN ENSAYOS COMO LIGANDO PARA DETECTAR UN ANALISIS SELECTIVO EN UNA MUESTRA FLUIDA, ES DESCRITO, DONDE LAS SALES DE TETRAZOLIUM AUMENTAN EL DESARROLLO DEL COLOR DE LOS SUSTRATOS DE ENZIMA INDIGOGENCIA EMPLEADOS EN LOS ENSAYOS. EL SISTEMA DE FASE SOLIDA COMPRENDE UN SOPORTE SOLIDO EN EL QUE SE LOCALIZA EN LA MISMA AREA UN RECPTOR Y UNA SAL DE TETRAZOLIUM. LOS METODOS PARA LOCALIZAR EL RECEPTOR EN EL SOPORTE SOLIDO INCLUYEN UNA LIGAZON QUIMICA Y CAPTURA DE MIROESFERAS CUBIERTAS DE RECPTOR. LAS SALES DE TETRAZOLIUM PUEDEN SER LOCALIZADAS EN EL SOPORTE SOLIDO POR ABSORCION POR INTERACCION HIDROFOBICA CON EL SOPORTE SOLIDO O POR CAPTURA DE LAS MICROESFERAS CUBIERTAS DE SALES DE TETRAZOLIUM. EL SISTEMA DE FASE SOLIDA ES EMPLEABLE CUANDO SE INCORPORA EN APARATOS PARA PROCESAR ENSAYOS DE RECEPCION DE LIGANDOS. EL USO DEL SISTEMA DE FASE SOLIDA EN INMUNOENSAYOS Y ENSAYOS DE ACIDOS NUCLEICOS ESTAN DESCRITOS.
ES198787306087T 1986-07-15 1987-07-09 Un sistema de fase solida que incorpora sales de tetrazolio para uso en ensayos de ligando-receptor. Expired - Lifetime ES2035067T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US88597386A 1986-07-15 1986-07-15

Publications (1)

Publication Number Publication Date
ES2035067T3 true ES2035067T3 (es) 1993-04-16

Family

ID=25388107

Family Applications (1)

Application Number Title Priority Date Filing Date
ES198787306087T Expired - Lifetime ES2035067T3 (es) 1986-07-15 1987-07-09 Un sistema de fase solida que incorpora sales de tetrazolio para uso en ensayos de ligando-receptor.

Country Status (8)

Country Link
EP (1) EP0253578B1 (es)
JP (1) JPS6330767A (es)
AT (1) ATE81723T1 (es)
AU (1) AU589021B2 (es)
CA (1) CA1289876C (es)
DE (1) DE3782282T2 (es)
ES (1) ES2035067T3 (es)
GR (1) GR3006185T3 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE86746T1 (de) * 1987-09-11 1993-03-15 Abbott Lab Geraet fuer die laterale immunochromatographische bestimmung.
US5279935A (en) * 1990-03-01 1994-01-18 Becton, Dickinson And Company Method of immunossay including deactivation of endogenous alkaline phosphatase
IT1248685B (it) * 1990-06-04 1995-01-26 Tecnogen Scpa Procedimento per la identificazione di sequenze polinucleotidiche e dispositivo relativo
WO1994006345A2 (en) * 1992-09-11 1994-03-31 Dambinova Svetlana Alexandrovn Immunosorbent for use in the diagnosis of neuro-psychological disorders, and use thereof
US6133436A (en) 1996-11-06 2000-10-17 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
CN102324290B (zh) * 2011-05-27 2013-02-27 广东风华高新科技股份有限公司 铜电极氧化锌压敏电阻器及其制备方法
AU2019221625A1 (en) * 2018-02-16 2020-08-13 Diagast In vitro diagnosis device comprising beads and uses thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3905872A (en) * 1973-12-14 1975-09-16 American Cyanamid Co Alkaline phosphatase test material
US4351899A (en) * 1978-07-19 1982-09-28 Owen Oliver E Semi-quantitative assay of metabolic acids
US4446232A (en) * 1981-10-13 1984-05-01 Liotta Lance A Enzyme immunoassay with two-zoned device having bound antigens
US4687732A (en) * 1983-06-10 1987-08-18 Yale University Visualization polymers and their application to diagnostic medicine
CA1272127A (en) * 1985-04-04 1990-07-31 Hybritech Incorporated Solid phase system for use in ligand-receptor assays

Also Published As

Publication number Publication date
JPS6330767A (ja) 1988-02-09
EP0253578A3 (en) 1988-07-20
DE3782282D1 (de) 1992-11-26
AU589021B2 (en) 1989-09-28
EP0253578A2 (en) 1988-01-20
GR3006185T3 (es) 1993-06-21
EP0253578B1 (en) 1992-10-21
AU7557087A (en) 1988-01-21
DE3782282T2 (de) 1993-03-18
ATE81723T1 (de) 1992-11-15
CA1289876C (en) 1991-10-01

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