ES2044784A1 - Procedimiento para la deteccion e identificacion de patogenos virales y subvirales. - Google Patents

Procedimiento para la deteccion e identificacion de patogenos virales y subvirales.

Info

Publication number
ES2044784A1
ES2044784A1 ES9201232A ES9201232A ES2044784A1 ES 2044784 A1 ES2044784 A1 ES 2044784A1 ES 9201232 A ES9201232 A ES 9201232A ES 9201232 A ES9201232 A ES 9201232A ES 2044784 A1 ES2044784 A1 ES 2044784A1
Authority
ES
Spain
Prior art keywords
viral
identification
procedure
detection
amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES9201232A
Other languages
English (en)
Other versions
ES2044784B1 (es
Inventor
Barbosa Nolasco Gustavo Nuno
Blas Beorlegui Carmen De
Tome M Jose Borja
Ascaso Fernando Ponz
Pascual Vicente Torres
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA
Original Assignee
Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA filed Critical Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA
Priority to ES09201232A priority Critical patent/ES2044784B1/es
Priority to JP13171793A priority patent/JP3343396B2/ja
Priority to DK93500079T priority patent/DK0574345T3/da
Priority to DE69333822T priority patent/DE69333822T2/de
Priority to EP93500079A priority patent/EP0574345B1/en
Priority to AT93500079T priority patent/ATE296900T1/de
Priority to PT93500079T priority patent/PT574345E/pt
Priority to AU41200/93A priority patent/AU681081B2/en
Priority to CA002098270A priority patent/CA2098270C/en
Publication of ES2044784A1 publication Critical patent/ES2044784A1/es
Application granted granted Critical
Publication of ES2044784B1 publication Critical patent/ES2044784B1/es
Priority to US08/389,067 priority patent/US5714312A/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

PROCEDIMIENTO PARA LA DETECCION E IDENTIFICACION DE PATOGENOS VIRALES Y SUBVIRALES. PARTE DE LA INMOVILIZACION DEL PATOGENO UTILIZANDO ANTICUERPOS FIJADOS A LA SUPERFICIE INTERNA DE LOS POCILLOS DE UNA PLACA DE MICROTITULACION, CON POSTERIOR SINTESIS, EN LA MISMA PLACA, DEL DNA COMPLEMENTARIO A UN FRAGMENTO DEL GENOMA DE PATOGENOS VIRALES O SUBVIRALES Y POSTERIOR AMPLIFICACION ENZIMATICA EN LA MISMA PLACA, PARA PROCEDER SEGUIDAMENTE A LA CUANTIFICACION DE LOS PRODUCTOS DE AMPLIFICACION EMPLEANDO TECNICAS ESPECTROFOTOMETRICAS, TAMBIEN REALIZADA EN LA MISMA PLACA, Y FINALMENTE LA IDENTIFICACION DE LOS PRODUCTOS DE AMPLIFICACION, MEDIANTE TECNICAS ELECTROFORETICAS.
ES09201232A 1992-06-12 1992-06-12 Procedimiento para la deteccion e identificacion de patogenos virales y subvirales. Expired - Fee Related ES2044784B1 (es)

Priority Applications (10)

Application Number Priority Date Filing Date Title
ES09201232A ES2044784B1 (es) 1992-06-12 1992-06-12 Procedimiento para la deteccion e identificacion de patogenos virales y subvirales.
JP13171793A JP3343396B2 (ja) 1992-06-12 1993-06-02 ウイルス性及びサブウイルス性病原体の検出及び同定方法
DE69333822T DE69333822T2 (de) 1992-06-12 1993-06-10 Verfahren für die Detektion und die Identifizierung von viralen und subviralen Pathogenen
EP93500079A EP0574345B1 (en) 1992-06-12 1993-06-10 Procedure for the detection and identification of viral and subviral pathogens
AT93500079T ATE296900T1 (de) 1992-06-12 1993-06-10 Verfahren für die detektion und die identifizierung von viralen und subviralen pathogenen
PT93500079T PT574345E (pt) 1992-06-12 1993-06-10 Processo para a deteccao e identificacao de patogenios virais e subvirais
DK93500079T DK0574345T3 (da) 1992-06-12 1993-06-10 Fremgangsmåde til påvisning og identifikation af virale og subvirale patogener
AU41200/93A AU681081B2 (en) 1992-06-12 1993-06-11 Procedure for the detection and identification of viral and subviral pathogens
CA002098270A CA2098270C (en) 1992-06-12 1993-06-11 Procedure for the detection and identification of viral and subviral pathogens
US08/389,067 US5714312A (en) 1992-06-12 1995-02-14 Procedure for the detection and identification of viral and subviral pathogens

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES09201232A ES2044784B1 (es) 1992-06-12 1992-06-12 Procedimiento para la deteccion e identificacion de patogenos virales y subvirales.

Publications (2)

Publication Number Publication Date
ES2044784A1 true ES2044784A1 (es) 1994-01-01
ES2044784B1 ES2044784B1 (es) 1994-09-01

Family

ID=8277329

Family Applications (1)

Application Number Title Priority Date Filing Date
ES09201232A Expired - Fee Related ES2044784B1 (es) 1992-06-12 1992-06-12 Procedimiento para la deteccion e identificacion de patogenos virales y subvirales.

Country Status (10)

Country Link
US (1) US5714312A (es)
EP (1) EP0574345B1 (es)
JP (1) JP3343396B2 (es)
AT (1) ATE296900T1 (es)
AU (1) AU681081B2 (es)
CA (1) CA2098270C (es)
DE (1) DE69333822T2 (es)
DK (1) DK0574345T3 (es)
ES (1) ES2044784B1 (es)
PT (1) PT574345E (es)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2078185A1 (es) * 1994-05-06 1995-12-01 Inia Procedimiento para la tipificacion molecular de patogenos virales con genoma rna y de patogenos subvirales mediante sintesis aleatoria de cdna.
WO1999058725A1 (es) * 1996-12-02 1999-11-18 Instituto Nacional De Investigacion Y Tecnologia Agraria Y Alimentaria (Inia) Procedimiento para la obtencion de fragmentos amplificados de acido nucleico de particulas virales y subvirales que contienen rna

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2074961B1 (es) * 1993-12-21 1996-04-01 Inia Procedimiento para analizar estructuralmente el genoma de patogenos virales y subvirales.
AR005140A1 (es) 1995-12-21 1999-04-14 Cornell Res Foundation Inc Una molecula de adn aislada que codifica una proteina o polipeptido de un virus del enrollamiento de la hoja de la vid, un sistema de expresion, unacelula huesped, un transplante de vastago o de rizoma transgenico que incluye dicha molecula de adn, metodo para producir dicho transplante, un metodo para
FI101159B (fi) * 1996-09-05 1998-04-30 Aboatech Ab Oy Menetelmä ja pakkaus mustaherukan suonenkatotaudin diagnosoimiseksi
ZA984232B (en) 1997-05-20 1998-12-01 Cornell Res Foundation Inc Grapevine leafroll virus type 2 proteins coding sequences and methods
KR100442987B1 (ko) * 2002-07-03 2004-08-04 학교법인 경희대학교 과수 바이러스 검정용 프라이머 및 이를 이용한 바이러스검출방법
KR100496012B1 (ko) * 2002-11-29 2005-06-16 대한민국 오이모자이크바이러스 진단용 특이 프라이머
GB0304832D0 (en) * 2003-03-04 2003-04-09 Secr Defence Assay method
CN1303424C (zh) * 2004-04-15 2007-03-07 云南省农业科学院 Cmv云南分离物tas－elisa检测试剂盒及其制备方法
KR100840736B1 (ko) * 2007-01-19 2008-06-24 대한민국(관리부서:농촌진흥청) 감자걀쪽바이로이드 진단용 프라이머
KR101161284B1 (ko) 2009-01-14 2012-08-01 대한민국 포도부채잎바이러스 피시알 검사 시스템
CN108504779A (zh) * 2018-04-27 2018-09-07 刘康康 一种桑花叶卷叶病中线虫传多面体病毒属病毒的试剂盒及其检测方法
WO2024047567A1 (en) * 2022-08-31 2024-03-07 VD-ING d.o.o. Method, device and system for detecting microbial or viral infection

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2537725A1 (fr) * 1982-12-09 1984-06-15 Pasteur Institut Procede de detection immunobacteriologique de germes pathogenes dans des milieux biologiques contamines
EP0145356A2 (en) * 1983-11-22 1985-06-19 National Research Development Corporation Method of assay of DNA or RNA from virus particles
EP0201184A2 (en) * 1985-03-28 1986-11-12 F. Hoffmann-La Roche Ag Process for amplifying nucleic acid sequences
EP0366448A2 (en) * 1988-10-25 1990-05-02 The General Hospital Corporation Method of detecting and identifying nucleic acid containing moieties
EP0469348A2 (en) * 1990-07-11 1992-02-05 SHIONOGI SEIYAKU KABUSHIKI KAISHA trading under the name of SHIONOGI & CO. LTD. cDNA sequence and detection of hepatits C virus

Family Cites Families (5)

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CA1253777A (en) * 1984-06-01 1989-05-09 Robert J. Carrico Nucleic acid hybridization assay employing immobilized rna probes
IE64040B1 (en) * 1989-06-15 1995-06-28 Akzo Nv Method for determining nucleic acid
AU7224591A (en) * 1990-01-24 1991-08-21 United States of America, as represented by the Secretary, U.S. Department of Commerce, The A rapid and sensitive test for detecting hepatitis a virus
WO1991015599A1 (en) * 1990-04-05 1991-10-17 Amrad Corporation Limited A method for detecting dna
IE911534A1 (en) * 1990-05-04 1991-11-06 Chiron Corp Protein-nucleic acid probes and immunoassays using same

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2537725A1 (fr) * 1982-12-09 1984-06-15 Pasteur Institut Procede de detection immunobacteriologique de germes pathogenes dans des milieux biologiques contamines
EP0145356A2 (en) * 1983-11-22 1985-06-19 National Research Development Corporation Method of assay of DNA or RNA from virus particles
EP0201184A2 (en) * 1985-03-28 1986-11-12 F. Hoffmann-La Roche Ag Process for amplifying nucleic acid sequences
EP0366448A2 (en) * 1988-10-25 1990-05-02 The General Hospital Corporation Method of detecting and identifying nucleic acid containing moieties
EP0469348A2 (en) * 1990-07-11 1992-02-05 SHIONOGI SEIYAKU KABUSHIKI KAISHA trading under the name of SHIONOGI & CO. LTD. cDNA sequence and detection of hepatits C virus

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ANN. APPL. BIOL., Vol. 117, 1990, VUNSH, R. ET AL.: "THE USE OF POLIMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF BEAN YELLOW MOSAIC VIRUS IN GLADIOLUS", pg. 561-569 *
J. OF VIROL METH., Vol. 33, 1991, WETZEL ET AL.: "A POLIMERASE CHAIN REACTION ASSAY ADAPTED TO PLUM POX POTYVIRUS DETECTION", pg. 355-365 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2078185A1 (es) * 1994-05-06 1995-12-01 Inia Procedimiento para la tipificacion molecular de patogenos virales con genoma rna y de patogenos subvirales mediante sintesis aleatoria de cdna.
WO1999058725A1 (es) * 1996-12-02 1999-11-18 Instituto Nacional De Investigacion Y Tecnologia Agraria Y Alimentaria (Inia) Procedimiento para la obtencion de fragmentos amplificados de acido nucleico de particulas virales y subvirales que contienen rna

Also Published As

Publication number Publication date
EP0574345B1 (en) 2005-06-01
CA2098270A1 (en) 1993-12-13
EP0574345A2 (en) 1993-12-15
US5714312A (en) 1998-02-03
JPH0662900A (ja) 1994-03-08
EP0574345A3 (en) 1995-06-28
DE69333822D1 (de) 2005-07-07
JP3343396B2 (ja) 2002-11-11
ATE296900T1 (de) 2005-06-15
PT574345E (pt) 2005-08-31
ES2044784B1 (es) 1994-09-01
AU4120093A (en) 1993-12-23
DK0574345T3 (da) 2005-10-03
AU681081B2 (en) 1997-08-21
CA2098270C (en) 2003-09-23
DE69333822T2 (de) 2006-05-11

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Legal Events

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FD1A Patent lapsed

Effective date: 20100315