ES2047484T3 - Un metodo para determinar cuantitativamente el 3-oxo-5-beta-esteroide y reactivo para el mismo. - Google Patents

Un metodo para determinar cuantitativamente el 3-oxo-5-beta-esteroide y reactivo para el mismo.

Info

Publication number
ES2047484T3
ES2047484T3 ES87111260T ES87111260T ES2047484T3 ES 2047484 T3 ES2047484 T3 ES 2047484T3 ES 87111260 T ES87111260 T ES 87111260T ES 87111260 T ES87111260 T ES 87111260T ES 2047484 T3 ES2047484 T3 ES 2047484T3
Authority
ES
Spain
Prior art keywords
oxo
beta
steroid
reactive
dehydrogenase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES87111260T
Other languages
English (en)
Inventor
Koji Ushizawa
Takae Shigihara
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sekisui Medical Co Ltd
Original Assignee
Daiichi Pure Chemicals Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Daiichi Pure Chemicals Co Ltd filed Critical Daiichi Pure Chemicals Co Ltd
Application granted granted Critical
Publication of ES2047484T3 publication Critical patent/ES2047484T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Steroid Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

UN ANALISIS CUANTITATIVO DE 3-OXO-5 BETA- ESTEROIDE QUE COMPRENDE LA ACTUACION DE LA 3-OXO-5 BETA- ESTEROIDE A4 DESHIDROGENASA SOBRE UNA MUESTRA EN PRESENCIA DE UN AGENTE CROMOFORO REDUCTOR Y MEDIDA DE LA DENSIDAD OPTICA DE LA SUSTANCIA CROMOFORA PRODUCIDA CON ESO. UN AGENTE CROMOFORO REDUCTOR TIPICO ES UN COMPUESTO DE TETRAZOLIO. LA INVENCION TAMBIEN PROPORCIONA UN REACTIVO PARA EL ANALISIS CUANTITATIVO DEL 3-OXO-5 BETAESTEROIDE QUE COMPRENDE 3-OXO-5 BETA- ESTEROIDE A4 DESHIDROGENASA Y UN COMPUESTO DE TETRAZOLIO. EL ANALISIS Y EL REACTIVO PROPORCIONAN UN METODO FACIL Y FIABLE PARA EL ENSAYO DE LA FUNCION HEPATICA.
ES87111260T 1986-11-11 1987-08-04 Un metodo para determinar cuantitativamente el 3-oxo-5-beta-esteroide y reactivo para el mismo. Expired - Lifetime ES2047484T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP61268313A JP2534044B2 (ja) 1986-11-11 1986-11-11 ３−オキソ−５β−ステロイドの定量法及びその定量用試薬

Publications (1)

Publication Number Publication Date
ES2047484T3 true ES2047484T3 (es) 1994-03-01

Family

ID=17456797

Family Applications (1)

Application Number Title Priority Date Filing Date
ES87111260T Expired - Lifetime ES2047484T3 (es) 1986-11-11 1987-08-04 Un metodo para determinar cuantitativamente el 3-oxo-5-beta-esteroide y reactivo para el mismo.

Country Status (7)

Country Link
US (1) US5085989A (es)
EP (1) EP0269786B1 (es)
JP (1) JP2534044B2 (es)
AT (1) ATE96847T1 (es)
DE (1) DE3788044T2 (es)
ES (1) ES2047484T3 (es)
NO (1) NO176212C (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2021207019A1 (en) * 2020-01-16 2022-09-01 Jsr Corporation Composition for producing bile acids

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3791933A (en) * 1971-02-25 1974-02-12 Geomet Rapid methods for assay of enzyme substrates and metabolites
GB1397406A (en) * 1971-06-22 1975-06-11 Nyegaard & Co As Enzymic reagent
JPS56151499A (en) * 1980-04-09 1981-11-24 Nyegaard & Co As Reagent system for measuring hydroxysteroid
DE3048662A1 (de) * 1980-12-23 1982-07-22 Boehringer Mannheim Gmbh, 6800 Mannheim Stabilisierte zubereitung von tetrazoliumsalzen
EP0245528B1 (en) * 1985-03-28 1991-07-24 Daiichi Pure Chemicals Co. Ltd. Quantitative analysis of 3 alpha-hydroxysteroid and reagent useful therefor
JPS61225000A (ja) * 1985-03-28 1986-10-06 Dai Ichi Pure Chem Co Ltd ３α―ヒドロキシステロイドの定量法及びこれに用いる試薬

Also Published As

Publication number Publication date
NO873348D0 (no) 1987-08-11
NO176212C (no) 1995-02-22
JPS63123400A (ja) 1988-05-27
NO873348L (no) 1988-05-13
DE3788044T2 (de) 1994-06-01
ATE96847T1 (de) 1993-11-15
JP2534044B2 (ja) 1996-09-11
EP0269786A1 (en) 1988-06-08
DE3788044D1 (de) 1993-12-09
US5085989A (en) 1992-02-04
NO176212B (no) 1994-11-14
EP0269786B1 (en) 1993-11-03

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