ES2056028A1 - Procedimiento para la seleccion de oligonucleotidos para la amplificacion especifica de genomas altamente variables. - Google Patents
Procedimiento para la seleccion de oligonucleotidos para la amplificacion especifica de genomas altamente variables.Info
- Publication number
- ES2056028A1 ES2056028A1 ES09300320A ES9300320A ES2056028A1 ES 2056028 A1 ES2056028 A1 ES 2056028A1 ES 09300320 A ES09300320 A ES 09300320A ES 9300320 A ES9300320 A ES 9300320A ES 2056028 A1 ES2056028 A1 ES 2056028A1
- Authority
- ES
- Spain
- Prior art keywords
- genomes
- oligonucleotides
- procedure
- selection
- specific
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title abstract 5
- 108091034117 Oligonucleotide Proteins 0.000 title abstract 4
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title abstract 4
- 230000003321 amplification Effects 0.000 title abstract 3
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 3
- 241000710198 Foot-and-mouth disease virus Species 0.000 abstract 1
- 238000004458 analytical method Methods 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 230000001717 pathogenic effect Effects 0.000 abstract 1
- 238000011895 specific detection Methods 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PROCEDIMIENTO PARA LA SELECCION DE OLIGONUCLEOTIDOS PARA LA AMPLIFICACION ESPECIFICA DE GENOMAS ALTAMENTE VARIABLES. EL PROCEDIMIENTO OBJETO DE LA PRESENTE INVENCION CONSISTE EN QUE MEDIANTE UNA SUCESION ESPECIFICA DE OPERACIONES SE LOCALIZAN Y SELECCIONAN REGIONES IDONEAS QUE PERMITAN LA AMPLIFICACION ESPECIFICA DE GRUPOS DE GENOMAS ALTAMENTE VARIABLES MEDIANTE EL USO DE POLIMERASAS TERMOESTABLES (RCP). EL PROCEDIMIENTO SE BASA EN UNA COMBINACION DE CRITERIOS TERMODINAMICOS Y CONSIDERACIONES SOBRE LA VARIABILIDAD DE LOS GENOMAS A COMPARAR. DE ESTA MANERA SE SELECCIONAN OLIGONUCLEOTIDOS ALTAMENTE CONSERVADOS ENTRE EL GRUPO DE GENOMAS QUE SE PRETENDE AMPLIFICAR Y QUE PRESENTAN, A SU VEZ, EL MAYOR NUMERO POSIBLE DE DIFERENCIAS DE NUCLEOTIDO FRENTE A OTROS GENOMAS ESPECIFICOS. LA APLICACION DE LA INVENCION AL ANALISIS DE SECUENCIAS GENOMICAS DEL VIRUS DE LA FIEBRE AFTOSA HA PERMITIDO LA SELECCION DE OLIGONUCLEOTIDOS QUE HACEN POSIBLE LA DETECCION ESPECIFICA DE ESTE GENOMA PATOGENO MEDIANTE LATECNICA DE RCP.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES09300320A ES2056028B1 (es) | 1993-02-18 | 1993-02-18 | Procedimiento para la seleccion de oligonucleotidos para la amplificacion especifica de genomas altamente variables. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES09300320A ES2056028B1 (es) | 1993-02-18 | 1993-02-18 | Procedimiento para la seleccion de oligonucleotidos para la amplificacion especifica de genomas altamente variables. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2056028A1 true ES2056028A1 (es) | 1994-09-16 |
| ES2056028B1 ES2056028B1 (es) | 1995-04-01 |
Family
ID=8280828
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES09300320A Expired - Fee Related ES2056028B1 (es) | 1993-02-18 | 1993-02-18 | Procedimiento para la seleccion de oligonucleotidos para la amplificacion especifica de genomas altamente variables. |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES2056028B1 (es) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0238329A2 (en) * | 1986-03-19 | 1987-09-23 | Zeneca Limited | Improvements in genetic probes |
| EP0298656A1 (en) * | 1987-07-10 | 1989-01-11 | Stephen Thomas Reeders | Polynucleotide probes |
| EP0329822A2 (en) * | 1988-02-24 | 1989-08-30 | Cangene Corporation | Nucleic acid amplification process |
| EP0511750A1 (en) * | 1991-04-09 | 1992-11-04 | The Regents Of The University Of California | Repeat sequence chromosome specific nucleic acid probes |
-
1993
- 1993-02-18 ES ES09300320A patent/ES2056028B1/es not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0238329A2 (en) * | 1986-03-19 | 1987-09-23 | Zeneca Limited | Improvements in genetic probes |
| EP0298656A1 (en) * | 1987-07-10 | 1989-01-11 | Stephen Thomas Reeders | Polynucleotide probes |
| EP0329822A2 (en) * | 1988-02-24 | 1989-08-30 | Cangene Corporation | Nucleic acid amplification process |
| EP0511750A1 (en) * | 1991-04-09 | 1992-11-04 | The Regents Of The University Of California | Repeat sequence chromosome specific nucleic acid probes |
Also Published As
| Publication number | Publication date |
|---|---|
| ES2056028B1 (es) | 1995-04-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AR085952A2 (es) | Polinucleotidos cebadores | |
| Kovalskaya et al. | Functional human endogenous retroviral LTR transcription start sites are located between the R and U5 regions | |
| RU93058449A (ru) | Hcv геномные последовательности для диагностических и терапевтических целей | |
| DE69403642D1 (de) | 7-deazapurin modifizierte oligonukleotide | |
| ATE266737T1 (de) | Künstliche fehlhybridisierung | |
| NO971493L (no) | Virusmateriale og nukleotidfragmenter som er forbundet med multippel sklerose, og som kan anvendes til diagnostiske, preventive og terapeutiske formål | |
| DE69128077D1 (de) | Nuklein-säure amplifikation mit dns abhängigen rns polymerasen aktivität von rns-replikasen | |
| NO951307L (no) | Terapeutiske anti-HIV oligonukleotider | |
| KR960704033A (ko) | 인간 면역결핍 바이러스에 대한 활성을 갖는 올리고뉴클레오티드(Oligonucleotides with Activity against Humand Immunodeficiency Virus) | |
| ATE386821T1 (de) | Zusammensetzungen und methoden zur detektion von humanem immundefizienz virus 2 (hiv-2) | |
| DK0775745T3 (da) | Cathepsin G-inhiberende aptamerer | |
| van Belkum et al. | Genotyping Naegleria spp. and Naegleria fowleri isolates by interrepeat polymerase chain reaction | |
| WO2000029611A3 (de) | Neue primer und sonden zum nachweis von hiv | |
| ATE160354T1 (de) | Oligonukleotide mit anti-cytomegalovirus wirkung | |
| ATE174056T1 (de) | Verbesserung in zusammenhang mit dem nachweis von viren | |
| IL108779A0 (en) | Polyribozyme capable of conferring viral resistance to plants, and resistant plants producing said ribozyme | |
| ES2093554A1 (es) | Procedimientos de amplificacion de genoma y mezclas de oligonucleotidos iniciadores para la deteccion y la identificacion de agentes infecciosos relacionados. | |
| ES2048652A1 (es) | Procedimientos de amplificacion de genoma para la deteccion e identificacion de secuencias genomicas relacionadas. | |
| WO1996039500A3 (en) | Oligonucleotides specific for hepatitis c virus | |
| PE20001331A1 (es) | Identificacion de genotipos senv | |
| MD2149F1 (en) | Oligonucleotide primer for hepatitis C virus RNA detection | |
| TH28996B (th) | "โอลิโกนิวคลีโอไทด์จำเพาะสำหรับไวรัสตับอักเสบ c | |
| HUP0105157A2 (hu) | Eljárás BNYVV rezisztencia kifejlesztésére cukorrépa-növényben | |
| Dietz et al. | Use of primer pools for specific gene amplification | |
| GB9506298D0 (en) | Diagnostic system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FD2A | Announcement of lapse in spain |
Effective date: 20180806 |