ES2065333T3 - Vector de clonacion de adn con plasmidos escindibles in vivo. - Google Patents

Vector de clonacion de adn con plasmidos escindibles in vivo.

Info

Publication number
ES2065333T3
ES2065333T3 ES88300224T ES88300224T ES2065333T3 ES 2065333 T3 ES2065333 T3 ES 2065333T3 ES 88300224 T ES88300224 T ES 88300224T ES 88300224 T ES88300224 T ES 88300224T ES 2065333 T3 ES2065333 T3 ES 2065333T3
Authority
ES
Spain
Prior art keywords
dna
cartridge
vivo
scindible
plasmids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES88300224T
Other languages
English (en)
Inventor
Joseph A M D Sorge
William M D Huse
Jay M Short
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Stratagene California
Original Assignee
Stratagene California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stratagene California filed Critical Stratagene California
Application granted granted Critical
Publication of ES2065333T3 publication Critical patent/ES2065333T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

SE DESCRIBEN VECTORES QUE EVITAN LOS PROCEDIMIENTOS TRADICIONALES DE CLONACION Y SUBCLONACION Y QUE CONTIENEN UN UNICO CARTUCHO DE ADN QUE PERMITE TANTO LA CLONACION DIRECTA DE ADN EN SECUANCIAS DE ADN PRESENTES EN EL CARTUCHO COMO LA ELIMINACION IN VIVO Y LA CIRCULACION DEL CARTUCHO, RINDIENDO ASI UNA ESTRUCTURA AUTONOMA DE REPLICACION. DADO QUE EL CARTUCHO DE ADN PUEDE CONTENER UNA AMPLIA VARIEDAD DE SECUENCIAS FUNCIONALES DE ADN, EL ADN CLONADO PUEDE SER SOMETIDO A UN GRAN NUMERO DE PROCEDIMIENTOS DE BIOLOGIA MOLECULAR SIN TEBER QUE ELIMINAR EL ADN CLONADO DEL CARTUCHO Y DE ESTA FORMA OBVIANDO LA NECESIDAD DE TECNICAS ADICIONALES DE SUBCLONACION. UN EJEMPLO PARTICULARMENTE UTIL DE ESTE TIPO DE VECTOR ES EL CARTUCHO ADN QUE CONTIENE EL BACTERIOFAGO LAMBDA.
ES88300224T 1987-01-12 1988-01-12 Vector de clonacion de adn con plasmidos escindibles in vivo. Expired - Lifetime ES2065333T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US244187A 1987-01-12 1987-01-12

Publications (1)

Publication Number Publication Date
ES2065333T3 true ES2065333T3 (es) 1995-02-16

Family

ID=21700777

Family Applications (1)

Application Number Title Priority Date Filing Date
ES88300224T Expired - Lifetime ES2065333T3 (es) 1987-01-12 1988-01-12 Vector de clonacion de adn con plasmidos escindibles in vivo.

Country Status (5)

Country Link
EP (1) EP0286200B1 (es)
AT (1) ATE112803T1 (es)
DE (1) DE3851770T2 (es)
ES (1) ES2065333T3 (es)
WO (1) WO1988005085A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5427908A (en) * 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
US5723286A (en) * 1990-06-20 1998-03-03 Affymax Technologies N.V. Peptide library and screening systems
US5270170A (en) * 1991-10-16 1993-12-14 Affymax Technologies N.V. Peptide library and screening method
US5733731A (en) * 1991-10-16 1998-03-31 Affymax Technologies N.V. Peptide library and screening method
AU731647B2 (en) * 1996-08-27 2001-04-05 Atsunobu Hiraoka Hematopoietic stem cell growth factor (SCGF)
FR2831185A1 (fr) * 2001-10-19 2003-04-25 Genoplante Valor Procede et kit de conversion de vecteurs hybrides de clonage en phase solide et procede de clonage de sequences nucleotidiques d'interet en faisant application

Also Published As

Publication number Publication date
DE3851770T2 (de) 1995-02-16
EP0286200A2 (en) 1988-10-12
DE3851770D1 (de) 1994-11-17
ATE112803T1 (de) 1994-10-15
EP0286200A3 (en) 1990-03-21
WO1988005085A1 (en) 1988-07-14
EP0286200B1 (en) 1994-10-12

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