ES2067703T3 - Cepa de saccharomyces cerevisiae productora de una proteina heterologa y procedimiento de preparacion de dicha proteina heterologa mediante fermentacion de dicha cepa. - Google Patents

Cepa de saccharomyces cerevisiae productora de una proteina heterologa y procedimiento de preparacion de dicha proteina heterologa mediante fermentacion de dicha cepa.

Info

Publication number
ES2067703T3
ES2067703T3 ES90400839T ES90400839T ES2067703T3 ES 2067703 T3 ES2067703 T3 ES 2067703T3 ES 90400839 T ES90400839 T ES 90400839T ES 90400839 T ES90400839 T ES 90400839T ES 2067703 T3 ES2067703 T3 ES 2067703T3
Authority
ES
Spain
Prior art keywords
strain
saccharomyces cerevisiae
protein
heterological
heterological protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90400839T
Other languages
English (en)
Inventor
Yves Lemoine
Martine Nguyen
Tilman Achstetter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Transgene SA
Original Assignee
Transgene SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transgene SA filed Critical Transgene SA
Application granted granted Critical
Publication of ES2067703T3 publication Critical patent/ES2067703T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C12N1/185—Saccharomyces isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

LA INVENCION SE REFIERE A UNA CEPA NUEVA DE SACAROMICES CEREVISAE PRODUCTORA DE UNA PROTEINA HETEROLOGA, Y MAS PARTICULARMENTE DE HIRUDINA, CARACTERIZADA POR UNA SUPRESION DE LA FUNCION PROTEOLITICA CODIFICADA POR EL GENERO PRC1. MAS PRECISAMENTE, ESTA DEFICIENCIA ES DEBIDA A UNA MUTACION O MAS ESPECIALMENTE A UN DETERIORO DEL GENERO DE ESTRUCTURA PRC1.
ES90400839T 1989-03-31 1990-03-28 Cepa de saccharomyces cerevisiae productora de una proteina heterologa y procedimiento de preparacion de dicha proteina heterologa mediante fermentacion de dicha cepa. Expired - Lifetime ES2067703T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR8904306A FR2645175B1 (fr) 1989-03-31 1989-03-31 Souche de saccharomyces cerevisiaeproductrice d'une proteine heterologue et procede de preparation de ladite proteine heterologue par fermentation de ladite souche

Publications (1)

Publication Number Publication Date
ES2067703T3 true ES2067703T3 (es) 1995-04-01

Family

ID=9380296

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90400839T Expired - Lifetime ES2067703T3 (es) 1989-03-31 1990-03-28 Cepa de saccharomyces cerevisiae productora de una proteina heterologa y procedimiento de preparacion de dicha proteina heterologa mediante fermentacion de dicha cepa.

Country Status (11)

Country Link
US (1) US5162208A (es)
EP (1) EP0390676B1 (es)
JP (1) JP3152918B2 (es)
AT (1) ATE117371T1 (es)
CA (1) CA2013240C (es)
DE (1) DE69016075T2 (es)
DK (1) DK0390676T3 (es)
ES (1) ES2067703T3 (es)
FR (1) FR2645175B1 (es)
GR (1) GR3015605T3 (es)
PT (1) PT93615B (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3583361D1 (de) * 1984-06-14 1991-08-08 Ucp Gen Pharma Ag Verfahren zur herstellung von thrombin-inhibitoren.
GB8907110D0 (en) * 1988-05-04 1989-05-10 Ciba Geigy Ag Improvements in the production of polypeptides
EP0548267A4 (en) * 1990-09-04 1994-11-23 Salk Inst Biotech Ind Production of insulin-like growth factor-1 in methylotrophic yeast cells
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
ATE220105T1 (de) * 1991-04-01 2002-07-15 Merck & Co Inc Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung
HU214698B (hu) * 1992-04-09 1998-06-29 Gyógyszerkutató Intézet Kft. Eljárás rekombináns deszulfátó-hirudin HV-1 peptidek előállítására
FR2692907B1 (fr) * 1992-06-25 1995-06-30 Rhone Poulenc Rorer Sa Levures kluyveromyces modifiees, preparation et utilisation.
DE69329540T2 (de) * 1992-09-04 2001-06-07 Novartis Ag, Basel Verfahren zur Herstellung von Proteaseinhibitoren
TW282490B (es) * 1992-12-15 1996-08-01 Ciba Geigy Ag
US5712114A (en) * 1995-06-06 1998-01-27 Basf Aktiengesellschaft Compositions for expression of proteins in host cells using a preprocollagen signal sequence
DE19529997C1 (de) 1995-08-16 1997-04-10 Hoechst Ag Verfahren zur Inaktivierung von Carboxypeptidase Y in hirudinhaltigen Kulturbrühen
DE69625623T2 (de) 1996-01-31 2003-11-06 Sumitomo Bakelite Co. Ltd., Tokio/Tokyo Verfahren zur Herstellung von in Epoxyharz eingekapselter Halbleitervorrichtung
RU2150501C1 (ru) * 1999-02-17 2000-06-10 Эльдаров Михаил Анатольевич Штамм дрожжей saccharomyces cerevisiae-bkm cr-349d-продуцент эпидермального фактора роста человека
US7795205B2 (en) * 2004-04-12 2010-09-14 Canyon Pharmaceuticals, Inc. Methods for effecting regression of tumor mass and size in a metastasized pancreatic tumor
KR100781386B1 (ko) 2005-10-14 2007-11-30 재단법인서울대학교산학협력재단 프로테인 다이설파이드아이소머라아제1를 코딩하는 유전자및 이알오1을 코딩하는 유전자를 동시 도입시킨 히루딘생산능 재조합 사카로마이세스 세레비지애 및 이를 이용한히루딘 단백질의 생산방법

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6236183A (ja) * 1985-06-20 1987-02-17 ザ・サルク・インステイチユ−ト・バイオテクノロジ−／インダストリアル・アソシエイツ・インコ−ポレ−テツド サツカロミセス・セレビシエからのポリペプチドの発現および分泌
MC1834A1 (fr) * 1986-07-10 1988-06-03 Transgene Sa Bloc fonctionnel d'adn et plasmide codant pour l'hirudine,levure transformee,procede de preparation de l'hirudine,hirudine obtenue et son utilisation
FR2607517B2 (fr) * 1986-12-01 1989-12-22 Transgene Sa Vecteurs d'expression de variants de l'hirudine dans les levures, procede et produit obtenu
ES2204884T3 (es) * 1988-01-05 2004-05-01 Roche Diagnostics Gmbh Procedimiento para la obtencion de proteinas o productos genicos que contienen proteinas.
AU614121B2 (en) * 1988-05-04 1991-08-22 Novartis Ag Improvements in the production of polypeptides
FR2634495B1 (fr) * 1988-07-01 1990-10-26 Chambon Pierre Procede de preparation d'une proteine par des levures utilisant un systeme inductible, vecteurs et souches transformees correspondantes

Also Published As

Publication number Publication date
CA2013240A1 (fr) 1990-09-30
JPH0372868A (ja) 1991-03-28
EP0390676B1 (fr) 1995-01-18
ATE117371T1 (de) 1995-02-15
CA2013240C (fr) 2000-09-05
PT93615B (pt) 1996-08-30
DE69016075T2 (de) 1995-05-24
FR2645175B1 (fr) 1994-02-18
DK0390676T3 (da) 1995-03-20
JP3152918B2 (ja) 2001-04-03
DE69016075D1 (de) 1995-03-02
PT93615A (pt) 1990-11-20
GR3015605T3 (en) 1995-06-30
US5162208A (en) 1992-11-10
FR2645175A1 (fr) 1990-10-05
EP0390676A1 (fr) 1990-10-03

Similar Documents

Publication Publication Date Title
ES2067703T3 (es) Cepa de saccharomyces cerevisiae productora de una proteina heterologa y procedimiento de preparacion de dicha proteina heterologa mediante fermentacion de dicha cepa.
NO180593C (no) Fremgangsmåte for fremstilling, i Saccharomyces cerevisiae, av et heterologt protein i homogen form
NO903411L (no) Dental restitusjon.
EP0048159A3 (en) Novel carboxyalkyl peptides and thioethers and ethers of peptides as antihypertensive agents
AU4042789A (en) New secretory leader sequences
DK590085D0 (da) Bicycliske heteroarylpiperazinderivater og fremgangsmaade til deres fremstilling
AU5199386A (en) Free or low alcohol beer
CA1251462C (es)
NO159147C (no) Fremgangsmaate ved fremstilling av seigt sliteresistent formlegeme.
DE3872996D1 (de) Herstellung von tertiaerem butylalkohol.
NO851818L (no) Tetningsmasse og fremgangsmaate for fremstilling av denne.
FR2513122B1 (es)
EA199700310A1 (ru) Биологический способ получения соединений дипептидной природы
DK0396436T3 (da) Gærstammer til fremstilling af modne heterologe proteiner, især hirudin
EP0240224A3 (en) An alpha interferon analogue
ES2157963T3 (es) Nueva secuencia pro que permite la secrecion de proteinas heterologas a partir de una celula de levadura.
JPS5658488A (en) Refined "sake" with low alcohol content
ES518579A0 (es) Un metodo de producir una composicion pulverulenta, que contiene una serrapeptasa estabilizada.
JPS5329977A (en) Novel mono-nucleus mycelium of corilus species and its production
NO891451L (no) Fremgangsmaate for fremstilling av proteiner som n-terminalt begynner med prolin.
EP0382332A3 (en) Improved supersecreting mutants of saccharomyces cerevisiae
ES2052621T3 (es) Procedimiento para la preparacion de plasma deficiente en factor viii:c, y un plasma deficiente, asi obtenido.
AU3306984A (en) L-aminoacid oxidase from yeasts of the genus cryptococcus, their preparation and use
JPS57103808A (en) Manufacture of decorateve block, decorative concrete block, etc.
JPS5782150A (en) Stained glass

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 390676

Country of ref document: ES