ES2072238T1 - Adn-polimerasas que tienen modificado el sitio de fijacion de nucleotidos para secuenciar adn. - Google Patents

Adn-polimerasas que tienen modificado el sitio de fijacion de nucleotidos para secuenciar adn.

Info

Publication number
ES2072238T1
ES2072238T1 ES94203433T ES94203433T ES2072238T1 ES 2072238 T1 ES2072238 T1 ES 2072238T1 ES 94203433 T ES94203433 T ES 94203433T ES 94203433 T ES94203433 T ES 94203433T ES 2072238 T1 ES2072238 T1 ES 2072238T1
Authority
ES
Spain
Prior art keywords
dna
modified
polymerases
fixation site
nucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES94203433T
Other languages
English (en)
Other versions
ES2072238T3 (es
Inventor
Stanley Tabor
Charles C Richardson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harvard University
Original Assignee
Harvard University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/337,615 external-priority patent/US5614365A/en
Application filed by Harvard University filed Critical Harvard University
Publication of ES2072238T1 publication Critical patent/ES2072238T1/es
Application granted granted Critical
Publication of ES2072238T3 publication Critical patent/ES2072238T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

GEN MODIFICADO QUE CODIFICA UNA POLIMERASA DE ADN MODIFICADA EN EL QUE LA POLIMERASA MODIFICADA INCORPORA DIDEOXINUCLEOTIDOS AL MENOS 20-PLIEGUES MEJORES COMPARADOS CON LOS CORRESPONDIENTES DEOXINUCLEOTIDOS COMO ESTA COMPARADO CON LA CORRESPONDIENTE POLIMERASA DE ADN DE PRODUCCION NATURAL.
ES94203433T 1994-10-17 1994-11-24 Adn-polimerasas que tienen un sitio de union con nucleotidos modificados para la secuenciacion de adn. Expired - Lifetime ES2072238T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US32443794A 1994-10-17 1994-10-17
US08/337,615 US5614365A (en) 1994-10-17 1994-11-10 DNA polymerase having modified nucleotide binding site for DNA sequencing

Publications (2)

Publication Number Publication Date
ES2072238T1 true ES2072238T1 (es) 1995-07-16
ES2072238T3 ES2072238T3 (es) 1997-01-16

Family

ID=26984456

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94203433T Expired - Lifetime ES2072238T3 (es) 1994-10-17 1994-11-24 Adn-polimerasas que tienen un sitio de union con nucleotidos modificados para la secuenciacion de adn.

Country Status (12)

Country Link
EP (1) EP0655506B1 (es)
CN (1) CN100335621C (es)
AT (1) ATE143057T1 (es)
AU (1) AU4193396A (es)
CA (1) CA2201885C (es)
DE (2) DE69400567T2 (es)
DK (1) DK0655506T3 (es)
ES (1) ES2072238T3 (es)
FI (1) FI120354B (es)
GR (1) GR950300040T1 (es)
HU (1) HU220839B1 (es)
WO (1) WO1996012042A2 (es)

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US6015668A (en) * 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
US5912155A (en) * 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
US5885813A (en) * 1995-05-31 1999-03-23 Amersham Life Science, Inc. Thermostable DNA polymerases
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
US6001645A (en) * 1995-06-07 1999-12-14 Promega Corporation Thermophilic DNA polymerases from thermotoga neapolitana
US5773258A (en) * 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
EP0871775A4 (en) * 1995-09-08 2002-07-31 Life Technologies Inc CLONED DNA POLYMERASES FROM THERMOTOGA AND MUTANTS THEREOF
GB9524974D0 (en) * 1995-12-06 1996-02-07 Amersham Int Plc An improved method for dye-terminator (cycle) sequencing
DE69613856T2 (de) * 1995-12-15 2002-04-04 Amersham Pharmacia Biotech Inc., Piscataway Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität
US5888736A (en) * 1995-12-22 1999-03-30 Visible Genetics, Inc. Method, compositions and kit for detection and identification of microorganisms
US5789168A (en) * 1996-05-01 1998-08-04 Visible Genetics Inc. Method for amplification and sequencing of nucleic acid polymers
US5830657A (en) * 1996-05-01 1998-11-03 Visible Genetics Inc. Method for single-tube sequencing of nucleic acid polymers
DE19612684A1 (de) * 1996-03-29 1997-10-02 Gsf Forschungszentrum Umwelt Neue Verwendung extrem thermophiler DNA-Polymerasen
US6083699A (en) * 1996-05-01 2000-07-04 Visible Genetics Inc. Method for bi-directional sequencing of nucleic acid polymers
DE69709087T2 (de) * 1996-05-01 2002-04-25 Visible Genetics, Inc. Verfahren zum nachweis und zur identifizierung von mikroorganismen
US6214555B1 (en) 1996-05-01 2001-04-10 Visible Genetics Inc. Method compositions and kit for detection
US6413718B1 (en) 1996-05-01 2002-07-02 Visible Genetics Inc. Method for sequencing of nucleic acid polymers
US5827716A (en) * 1996-07-30 1998-10-27 Amersham Life Science, Inc. Modified Pol-II type DNA polymerases
CZ293215B6 (cs) * 1996-08-06 2004-03-17 F. Hoffmann-La Roche Ag Rekombinantní tepelně stálá DNA polymeráza, způsob její přípravy a prostředek, který ji obsahuje
WO1998023733A2 (en) 1996-11-27 1998-06-04 University Of Washington Thermostable polymerases having altered fidelity
US6306588B1 (en) 1997-02-07 2001-10-23 Invitrogen Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
US6265193B1 (en) * 1997-03-12 2001-07-24 Pe Corporation (Ny) DNA polymerases having improved labeled nucleotide incorporation properties
JP3318579B2 (ja) 1997-07-07 2002-08-26 理化学研究所 Ｄｎａの塩基配列決定方法
JP3172710B2 (ja) 1997-07-07 2001-06-04 理化学研究所 Ｒｎａポリメラーゼ
US6228628B1 (en) * 1997-07-09 2001-05-08 Roche Molecular Systems Mutant chimeric DNA polymerase
US5882904A (en) * 1997-08-04 1999-03-16 Amersham Pharmacia Biotech Inc. Thermococcus barossii DNA polymerase mutants
CA2243985C (en) * 1997-09-11 2004-08-10 F. Hoffmann-La Roche Ag Thermostable dna polymerases incorporating nucleoside triphosphates labeled with fluorescein family dyes
JP2002510466A (ja) * 1998-01-23 2002-04-09 アマーシャム・ファルマシア・バイオテック・インコーポレーテッド Ｄｎａ配列決定のための方法、試薬溶液およびキット
CA2266755A1 (en) * 1998-04-24 1999-10-24 James M. Dunn Method for sequencing of nucleic acid polymers
US6096875A (en) 1998-05-29 2000-08-01 The Perlein-Elmer Corporation Nucleotide compounds including a rigid linker
GB9907813D0 (en) * 1999-04-06 1999-06-02 Medical Biosystems Ltd Synthesis
JP2003507072A (ja) * 1999-08-21 2003-02-25 アマシャム・バイオサイエンス・コーポレイション 耐塩性を改善した、Ｅ６８１にアミノ酸置換を有するＴａｑＤＮＡポリメラーゼおよびその同族体
US20040009486A1 (en) 1999-10-29 2004-01-15 Sorge Joseph A. Compositions and methods utilizing DNA polymerases
JP4949585B2 (ja) 1999-10-29 2012-06-13 アジレント・テクノロジーズ・インク Ｄｎａポリメラーゼを使用した組成物および方法
WO2001038546A1 (en) * 1999-11-23 2001-05-31 Amersham Biosciences Corp Improving dideoxynucleotide-triphosphate utilization by the hyper-thermophilic dna polymerase from the archaeon pyrococcus furiosus
US6329178B1 (en) 2000-01-14 2001-12-11 University Of Washington DNA polymerase mutant having one or more mutations in the active site
US7179590B2 (en) 2000-04-18 2007-02-20 Roche Molecular Systems, Inc High temperature reverse transcription using mutant DNA polymerases
AU2007201277B2 (en) * 2000-05-24 2011-10-13 Third Wave Technologies, Inc. Detection of RNA
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US20050042629A1 (en) 2002-09-13 2005-02-24 Applera Corporation Thermus scotoductus nucleic acid polymerases
US7222059B2 (en) 2001-11-15 2007-05-22 Siemens Medical Solutions Diagnostics Electrophoretic trace simulator
AU2002346517A1 (en) * 2001-11-30 2003-06-17 Applera Corporation Thermus oshimai nucleic acid polymerases
US7052877B2 (en) 2001-11-30 2006-05-30 Applera Corporation Thermus brockianus nucleic acid polymerases
WO2003048309A2 (en) 2001-11-30 2003-06-12 Applera Corporation Thermus thermophilus nucleic acid polymerases
CA2485203A1 (en) * 2002-05-31 2003-12-11 University Of Washington Error-prone dna polymerase i mutants and methods for targeted random mutagenesis in continuous culture using error-prone dna polymerase i mutants
US7309570B2 (en) 2002-10-21 2007-12-18 University Of Texas Methods for using double-mutant RNA polymerases with reduced discrimination between non-canonical and canonical nucleoside triphosphates
US8304189B2 (en) 2003-12-01 2012-11-06 Life Technologies Corporation Nucleic acid molecules containing recombination sites and methods of using the same
BRPI0620445A2 (pt) 2005-12-23 2011-11-08 Siemens Healthcare Diagnostics Inc métodos e reagentes para genotipagem de hcv
US20100009412A1 (en) 2006-05-01 2010-01-14 Siemens Healthcare Diagnostics Inc. Novel Oligonucleotide Primers and Methods for DNA Replication
US10131898B2 (en) * 2014-07-22 2018-11-20 Bio-Rad Laboratories, Inc. Buffers for use with polymerases
CN106318924B (zh) * 2016-10-26 2018-10-16 江南大学 一种催化dna合成延伸能力提高的dna聚合酶
CN109652499B (zh) * 2017-10-12 2022-06-03 深圳华大智造科技股份有限公司 快速检测dna聚合酶3′-5′外切活性或错配的方法和试剂盒
CN110093410A (zh) * 2019-05-21 2019-08-06 通用生物系统（安徽）有限公司 一种dna测序反应试剂及其制备方法
CN119177225A (zh) 2019-11-13 2024-12-24 深圳市真迈生物科技有限公司 改造的Klenow片段及应用
CN111826366B (zh) * 2020-06-05 2022-08-02 广州英赞生物科技有限公司 一种直扩型热启动dna聚合酶及其制备方法与应用

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CA1338457C (en) * 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
US4942130A (en) * 1987-01-14 1990-07-17 President & Fellows Of Harvard College T7 DNA polymerase
US4962020A (en) * 1988-07-12 1990-10-09 President And Fellows Of Harvard College DNA sequencing
JPH0630640B2 (ja) * 1989-02-06 1994-04-27 イーストマン コダック カンパニー 熱安定性ポリメラーゼｄｎａシーケエンシング反応濃厚物
US5001050A (en) * 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase
US5108892A (en) * 1989-08-03 1992-04-28 Promega Corporation Method of using a taq dna polymerase without 5'-3'-exonuclease activity
CA2114124A1 (en) * 1991-07-24 1993-02-04 Keith Gregg Single step amplification and sequencing of nucleic acids

Also Published As

Publication number Publication date
DE69400567D1 (de) 1996-10-24
FI120354B (fi) 2009-09-30
DE655506T1 (de) 1995-09-28
ES2072238T3 (es) 1997-01-16
AU4193396A (en) 1996-05-06
EP0655506A1 (en) 1995-05-31
HUT77037A (hu) 1998-03-02
HU220839B1 (en) 2002-06-29
FI971611L (fi) 1997-06-13
CN1170439A (zh) 1998-01-14
CN100335621C (zh) 2007-09-05
FI971611A0 (fi) 1997-04-16
ATE143057T1 (de) 1996-10-15
EP0655506B1 (en) 1996-09-18
WO1996012042A2 (en) 1996-04-25
CA2201885A1 (en) 1996-04-25
CA2201885C (en) 2009-12-29
DK0655506T3 (da) 1997-03-10
GR950300040T1 (en) 1995-07-31
DE69400567T2 (de) 1997-02-06
WO1996012042A3 (en) 1996-08-01

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