ES2073409T3 - Dna conteniendo el codigo de la sintetasa del acv. - Google Patents
Dna conteniendo el codigo de la sintetasa del acv.Info
- Publication number
- ES2073409T3 ES2073409T3 ES88311655T ES88311655T ES2073409T3 ES 2073409 T3 ES2073409 T3 ES 2073409T3 ES 88311655 T ES88311655 T ES 88311655T ES 88311655 T ES88311655 T ES 88311655T ES 2073409 T3 ES2073409 T3 ES 2073409T3
- Authority
- ES
- Spain
- Prior art keywords
- acv
- penicillin
- gene
- hosts
- expression
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P37/00—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Compounds Of Unknown Constitution (AREA)
Abstract
Un procedimiento para la transformación de una célula huésped con un vector recombinante, el cual, comprende el mezclado conjunto del huésped y del vector recombinante bajo condiciones de transformación convencionales, caracterizado porque el vector recombinante comprende DNA, el cual, comprende, esencialmente, un gen que contiene el código de una enzima sintetasa capaz de generar (L-aminoadipil)-L-cisteinil-D-valina obtenible a partir de Penicillium chrysogenum, mediante: a) la construcción de una biblioteca genómica a partir de los fragmentos de DNA cromosómicos del P. chrysogenum, b) la realización de uno o más experimentos de hibridación con el fin de seleccionar, a partir de dicha biblioteca, los clones que contengan el DNA de la invención y c) el aislamiento del DNA de la invención, conteniendo dicho DNA la configuración de los sitios de restricción mostrados en la Figura 6(b).
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB878728811A GB8728811D0 (en) | 1987-12-09 | 1987-12-09 | Novel substance |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2073409T3 true ES2073409T3 (es) | 1995-08-16 |
Family
ID=10628254
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES88311655T Expired - Lifetime ES2073409T3 (es) | 1987-12-09 | 1988-12-09 | Dna conteniendo el codigo de la sintetasa del acv. |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP0320272B1 (es) |
| JP (1) | JP2970918B2 (es) |
| AT (1) | ATE122718T1 (es) |
| DE (1) | DE3853811T2 (es) |
| ES (1) | ES2073409T3 (es) |
| GB (1) | GB8728811D0 (es) |
| GR (1) | GR3017073T3 (es) |
| WO (1) | WO1989005350A1 (es) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5108918A (en) * | 1988-08-11 | 1992-04-28 | Gist-Brocades | Method for identifying and using biosynthetic genes for enhanced production of secondary metabolites |
| FI104264B (fi) * | 1988-08-11 | 1999-12-15 | Dsm Ip Assets Bv | Menetelmä sekundääristen metaboliittien tuoton parantamiseksi käyttämällä rykelmöityjä biosynteettisiä geenejä |
| US5882879A (en) * | 1990-02-28 | 1999-03-16 | Gist-Brocades, N.V. | Method for influencing β-lactam antibiotic production and for isolation of large quantities of ACV synthetase |
| DE69129869T2 (de) * | 1990-02-28 | 1999-01-14 | Gist-Brocades N.V., Delft | Verfahren zur Beeinflussung der Produktion von beta-lactam Antibiotika und zur Isolierung grosser Mengen von ACV-Synthetase |
| US6322998B1 (en) | 1990-06-01 | 2001-11-27 | Eli Lilly And Company | Recombinant DNA compounds that encode ACV synthetase activity of Cephalosporium acremonium |
| US5589571A (en) * | 1994-10-28 | 1996-12-31 | Cor Therapeutics, Inc. | Process for production of inhibited forms of activated blood factors |
| EP1819813A2 (en) * | 2004-12-10 | 2007-08-22 | DSMIP Assets B.V. | Production of beta-lactam antibiotics by genetically modified non-naturally producing microorganisms |
| US20100048938A1 (en) * | 2006-06-22 | 2010-02-25 | Marco Alexander Van Den Berg | Fermentation of pravastatin |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59111699A (ja) * | 1982-12-17 | 1984-06-27 | 富士通株式会社 | 話者認識方式 |
| IL78531A0 (en) * | 1985-04-22 | 1986-08-31 | Lilly Co Eli | Recombinant dna expression vectors and dna compounds which encode isopenicillin n synthetase |
| US4892819A (en) * | 1985-11-25 | 1990-01-09 | Eli Lilly And Company | Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from penicillium chrysogenum |
| DE3789880T2 (de) * | 1986-01-31 | 1994-09-08 | Beecham Group Plc | Isolierung und Expression von bei der Biosynthese von Beta-Laktamen beteiligten Genen. |
| IL85535A (en) * | 1987-03-04 | 1994-02-27 | Lilly Co Eli | Recombinant dna expression vectors and dna compounds that encode deacetoxycephalosporin c synthetase and deacetylcephalosporin c synthetase |
| GB2208077A (en) * | 1987-04-24 | 1989-02-22 | Antibioticos Sa | Isopenicillin n synthetase gene derived from aspergillus nidulans |
-
1987
- 1987-12-09 GB GB878728811A patent/GB8728811D0/en active Pending
-
1988
- 1988-12-09 JP JP1500399A patent/JP2970918B2/ja not_active Expired - Lifetime
- 1988-12-09 DE DE3853811T patent/DE3853811T2/de not_active Expired - Lifetime
- 1988-12-09 WO PCT/GB1988/001083 patent/WO1989005350A1/en not_active Ceased
- 1988-12-09 EP EP88311655A patent/EP0320272B1/en not_active Expired - Lifetime
- 1988-12-09 ES ES88311655T patent/ES2073409T3/es not_active Expired - Lifetime
- 1988-12-09 AT AT88311655T patent/ATE122718T1/de not_active IP Right Cessation
-
1995
- 1995-08-09 GR GR950402201T patent/GR3017073T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| DE3853811T2 (de) | 1995-10-12 |
| JPH02502606A (ja) | 1990-08-23 |
| DE3853811D1 (de) | 1995-06-22 |
| GB8728811D0 (en) | 1988-01-27 |
| EP0320272A1 (en) | 1989-06-14 |
| ATE122718T1 (de) | 1995-06-15 |
| EP0320272B1 (en) | 1995-05-17 |
| GR3017073T3 (en) | 1995-11-30 |
| JP2970918B2 (ja) | 1999-11-02 |
| WO1989005350A1 (en) | 1989-06-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Smith et al. | Cloning and heterologous expression of the penicillin biosynthetic gene cluster from Penicillium chrysogenum | |
| US20060127972A1 (en) | In vivo production of cephalosporins | |
| RU2002130702A (ru) | Новый биологический способ получения 7-ацк и 7-адац | |
| DE69227494D1 (de) | Neues biologisches Verfahren zur Herstellung von 7-ADCA | |
| Martin et al. | Molecular genetics of penicillin and cephalosporin antibiotic biosynthesis | |
| Queener | Molecular biology of penicillin and cephalosporin biosynthesis | |
| ES2073409T3 (es) | Dna conteniendo el codigo de la sintetasa del acv. | |
| HUT46068A (en) | Gene technological process for producing trypsin inhibitor produced in pancreas by means of recombinant host organism | |
| KR910018550A (ko) | 페니실린 g 아실라아제, 이 효소를 코드화한 유전자 및 이 효소의 제조 방법 | |
| FI102971B (fi) | Enantioselektiivinen polypeptidi, sen valmistus, sitä koodittava DNA j a geeni, sekä sitä ilmentävä mikro-organismi, ja menetelmä stereoisome erin valmistamiseksi | |
| ES2086386T3 (es) | Procedimiento para producir compuestos de 7-aminocefem o sus sales. | |
| HUT53149A (en) | Process for producing dna encoding isopenicillin n-epimerase (racemase) and vector expressing recombinant dna | |
| PT81265A (en) | Improved process for the production of antibiotic derivatives of a-21978c cyclic peptides | |
| KR900700610A (ko) | β-락탐 화합물의 생성을 위한 유전자 및 유전자 생성물 | |
| DE69121983D1 (de) | Verfahren zur Herstellung von Ascorbinsäure-2-Phosphat | |
| GR3031739T3 (en) | Improved process for preparing cephalosporins. | |
| KR930013111A (ko) | 바실러스의 파라-니트로벤질 에스테라제를 암호화하는 재조합 dna 화합물 및 발현벡터 | |
| EP0235112A3 (en) | The gal operon of streptomyces | |
| DE3476423D1 (en) | Biosynthesis of unnatural cephalosporins | |
| ES2108651A1 (es) | Procedimiento para incrementar la produccion de penicilina g (bencilpenicilina) en penicillium chrysogenum mediante la expresion del gen pcl. | |
| Ghosh | Strategies for manipulation of genes in microbes for production of β-lactam antibiotics | |
| PT96912A (pt) | Processo para influenciar a producao de antibioticos de beta-lactama e para o isolamento de grandes quantidades de sintetase de acv | |
| JPS6451083A (en) | Production of acidic urease | |
| BG50560A1 (en) | Method for cephasoline production |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
Ref document number: 320272 Country of ref document: ES |