ES2076152T3 - Definicion y estructura de genes para proteasa a y proteasa b procedente de streptomices griseus. - Google Patents
Definicion y estructura de genes para proteasa a y proteasa b procedente de streptomices griseus.Info
- Publication number
- ES2076152T3 ES2076152T3 ES88111713T ES88111713T ES2076152T3 ES 2076152 T3 ES2076152 T3 ES 2076152T3 ES 88111713 T ES88111713 T ES 88111713T ES 88111713 T ES88111713 T ES 88111713T ES 2076152 T3 ES2076152 T3 ES 2076152T3
- Authority
- ES
- Spain
- Prior art keywords
- protease
- signal sequence
- dna
- protein
- griseus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 7
- 108091005804 Peptidases Proteins 0.000 title abstract 3
- 239000004365 Protease Substances 0.000 title abstract 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 title abstract 3
- 108010076504 Protein Sorting Signals Proteins 0.000 abstract 8
- 102000004169 proteins and genes Human genes 0.000 abstract 4
- 241000187392 Streptomyces griseus Species 0.000 abstract 2
- 230000028327 secretion Effects 0.000 abstract 2
- 102000004316 Oxidoreductases Human genes 0.000 abstract 1
- 108090000854 Oxidoreductases Proteins 0.000 abstract 1
- 101710180012 Protease 7 Proteins 0.000 abstract 1
- 241000187747 Streptomyces Species 0.000 abstract 1
- 241000892502 Streptomyces lividans 1326 Species 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 230000002255 enzymatic effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/53—Colony-stimulating factor [CSF]
- C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
UNA SECUENCIA DE SEÑAL DE DNA INICIALMENTE AISLADA DE STREPTOMYCES GRISEUS CODIFICA UN PEPTIDO SEÑAL QUE CONTROLA LA SECRECION, VIA UN INTERMEDIO FUSIONADO DE UNA PROTEINA A PARTIR DE LA CELULA EN LA QUE EXPRESA LA SECUENCIA DE SEÑAL DNA. LA SECUENCIA DE SEÑAL PROCEDE DE GENES QUE CODIFICAN PROTEASA A Y B DE S. GRISEUS. LA SECUENCIA DE SEÑAL DNA CODIFICA UN PEPTIDO SEÑAL DE 38 AMINOACIDOS. DEL PEPTIDO SEÑAL RESULTA UN DNA CONSTRUIDO, INCLUYENDO LA SECUENCIA DE SEÑAL DNA Y UNA SECUENCIA DE GEN QUE CODIFICA UNA PROTEINA, CUANDO SE TRANSFORMA EN UNA CELULA VIVA POR UN VECTOR ADECUADO, QUE GOBIERNA CORRECTAMENTE LA SECRECION DE UNA PROTEINA MADURA DE ESTRUCTURA DESEADA, EN PARTICULAR DEL GENERO PROCARIOTICA, ELEGIDA POR SU CAPACIDAD PARA MANIFESTAR ACTIVIDAD ENZIMATICA DE UN TIPO TIPIFICADO POR, PERO NO EXCLUSIVO AL, DE LA PROTEINA DISULFURO OXIDORREDUCTORA, EC 5.3.4.1, MAS PARTICULARMENTE EN EL GENERO STREPTOMYCES, Y MAS EN ESPECIAL EN STREPTOMYCES LIVIDANS 66.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA000542678A CA1295566C (en) | 1987-07-21 | 1987-07-21 | Characterization and structure of genes for protease a and protease b from streptomyces griseus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2076152T3 true ES2076152T3 (es) | 1995-11-01 |
Family
ID=4136119
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES88111713T Expired - Lifetime ES2076152T3 (es) | 1987-07-21 | 1988-07-20 | Definicion y estructura de genes para proteasa a y proteasa b procedente de streptomices griseus. |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0300466B1 (es) |
| AT (1) | ATE127840T1 (es) |
| CA (1) | CA1295566C (es) |
| DE (1) | DE3854456T2 (es) |
| ES (1) | ES2076152T3 (es) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1295567C (en) * | 1988-07-25 | 1992-02-11 | Lawrence T. Malek | Expression system for the secretion of bioactive human granulocyte, macrophage-colony stimulating factor (gm-csf) and other heterologous proteins from streptomyces |
| FR2686899B1 (fr) | 1992-01-31 | 1995-09-01 | Rhone Poulenc Rorer Sa | Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant. |
| US5616485A (en) * | 1993-12-23 | 1997-04-01 | Cangene Corporation | Streptomyces proteases and improved streptomyces strains for expression of peptides and polypeptides |
| GB9526733D0 (en) | 1995-12-30 | 1996-02-28 | Delta Biotechnology Ltd | Fusion proteins |
| US6855548B2 (en) | 2000-02-08 | 2005-02-15 | F. Hoffman-La Roche Ag | Use of acid-stable proteases in animal feed |
| US6946134B1 (en) | 2000-04-12 | 2005-09-20 | Human Genome Sciences, Inc. | Albumin fusion proteins |
| JP2003530838A (ja) | 2000-04-12 | 2003-10-21 | ヒューマン ゲノム サイエンシズ インコーポレイテッド | アルブミン融合タンパク質 |
| US7507413B2 (en) | 2001-04-12 | 2009-03-24 | Human Genome Sciences, Inc. | Albumin fusion proteins |
| AU2002364587A1 (en) | 2001-12-21 | 2003-07-30 | Human Genome Sciences, Inc. | Albumin fusion proteins |
| ES2425738T3 (es) | 2001-12-21 | 2013-10-17 | Human Genome Sciences, Inc. | Proteínas de fusión de la albúmina |
| EP1594530A4 (en) | 2003-01-22 | 2006-10-11 | Human Genome Sciences Inc | HYBRID PROTEINS OF ALBUMIN |
| CN1774504B (zh) | 2003-02-07 | 2013-04-17 | 诺维信公司 | 蛋白酶 |
| WO2004072279A2 (en) * | 2003-02-07 | 2004-08-26 | Novozymes A/S | Proteases |
| ES2358092T3 (es) | 2003-10-10 | 2011-05-05 | Novozymes A/S | Variantes de proteasa. |
| FR2865216B1 (fr) | 2004-01-15 | 2006-05-19 | Agronomique Inst Nat Rech | Procede de surproduction d'une proteine recombinante determinee par des souches monocaryotiques de p. cinnabarinus |
| ES2380105T3 (es) | 2004-06-21 | 2012-05-08 | Novozymes A/S | Proteasas de Nocardiopsis |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4717666A (en) * | 1984-03-05 | 1988-01-05 | Smithkline Beckman Corporation | Cloned streptomycete lividans excretable β-galactosidase gene |
| DE3418274A1 (de) * | 1984-05-17 | 1985-11-21 | Hoechst Ag, 6230 Frankfurt | Signalpeptid fuer die exkretion von peptiden in streptomyceten |
| US4745056A (en) * | 1984-10-23 | 1988-05-17 | Biotechnica International, Inc. | Streptomyces secretion vector |
| US4783415A (en) * | 1985-03-28 | 1988-11-08 | Meiji Seika Kabushiki Kaisha | Gene coding for signal peptides and utilization thereof |
| JPS62122585A (ja) * | 1985-08-21 | 1987-06-03 | Sankyo Co Ltd | 宿主・ベクタ−系 |
| CA1295563C (en) * | 1985-11-01 | 1992-02-11 | Robert T. Garvin | Production of active proteins containing cystine residues |
-
1987
- 1987-07-21 CA CA000542678A patent/CA1295566C/en not_active Expired - Lifetime
-
1988
- 1988-07-20 EP EP88111713A patent/EP0300466B1/en not_active Expired - Lifetime
- 1988-07-20 DE DE3854456T patent/DE3854456T2/de not_active Expired - Fee Related
- 1988-07-20 AT AT88111713T patent/ATE127840T1/de not_active IP Right Cessation
- 1988-07-20 ES ES88111713T patent/ES2076152T3/es not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| DE3854456T2 (de) | 1996-05-09 |
| EP0300466A1 (en) | 1989-01-25 |
| DE3854456D1 (de) | 1995-10-19 |
| CA1295566C (en) | 1992-02-11 |
| ATE127840T1 (de) | 1995-09-15 |
| EP0300466B1 (en) | 1995-09-13 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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