ES2076318T3 - Control de la acumulacion de vectores de expresion aberrantes durante procedimientos de fermentacion. - Google Patents

Control de la acumulacion de vectores de expresion aberrantes durante procedimientos de fermentacion.

Info

Publication number
ES2076318T3
ES2076318T3 ES90306853T ES90306853T ES2076318T3 ES 2076318 T3 ES2076318 T3 ES 2076318T3 ES 90306853 T ES90306853 T ES 90306853T ES 90306853 T ES90306853 T ES 90306853T ES 2076318 T3 ES2076318 T3 ES 2076318T3
Authority
ES
Spain
Prior art keywords
expression
accumulation
aberrants
verses
control
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90306853T
Other languages
English (en)
Inventor
William Lawrence Muth
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eli Lilly and Co
Original Assignee
Eli Lilly and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eli Lilly and Co filed Critical Eli Lilly and Co
Application granted granted Critical
Publication of ES2076318T3 publication Critical patent/ES2076318T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Alcoholic Beverages (AREA)
  • Fertilizers (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

EL INVENTO SUMINISTRA UN METODO PARA EVITAR LA ACUMULACION DE VECTORES DE EXPRESION ANOMALOS DURANTE LOS PROCESOS DE FERMENTACION QUE RECAEN SOBRE LA EXPRESION DE UN GENE CODIFICADO DE UN VECTOR DE EXPRESION PARA LA PRODUCCION DE UN PRODUCTO POLIPEPTIDO DE INTERES. SE AÑADEN ANTIBIOTICOS EN APROXIMADAMENTE EL MOMENTO EN EL CUAL LA EXPRESION DEL PRODUCTO COMIENZA.
ES90306853T 1989-06-26 1990-06-22 Control de la acumulacion de vectores de expresion aberrantes durante procedimientos de fermentacion. Expired - Lifetime ES2076318T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US37157989A 1989-06-26 1989-06-26

Publications (1)

Publication Number Publication Date
ES2076318T3 true ES2076318T3 (es) 1995-11-01

Family

ID=23464539

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90306853T Expired - Lifetime ES2076318T3 (es) 1989-06-26 1990-06-22 Control de la acumulacion de vectores de expresion aberrantes durante procedimientos de fermentacion.

Country Status (13)

Country Link
US (1) US5824496A (es)
EP (1) EP0405858B1 (es)
JP (1) JPH0347081A (es)
AT (1) ATE125300T1 (es)
AU (1) AU617057B2 (es)
CA (1) CA2019696A1 (es)
DE (1) DE69020968T2 (es)
DK (1) DK0405858T3 (es)
ES (1) ES2076318T3 (es)
GR (1) GR3017703T3 (es)
HU (1) HUT54415A (es)
IE (1) IE67799B1 (es)
IL (1) IL94847A0 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5395761A (en) * 1991-11-27 1995-03-07 Eli Lilly And Company Plasmid pHKY334, an expression vector for EK-BGH and host cells transformed therewith
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4529695A (en) * 1981-06-18 1985-07-16 Wisconsin Alumni Research Foundation Recombinant cloning vector, host for the vector, and method for using same
CA1206433A (en) * 1982-08-06 1986-06-24 Channing R. Robertson Production of biological products using resting cells
EP0178764A1 (en) * 1984-08-28 1986-04-23 Genex Corporation Method of stabilizing a host microorganism/expression vector system for large scale production of proteins

Also Published As

Publication number Publication date
AU617057B2 (en) 1991-11-14
EP0405858A3 (en) 1992-04-29
IL94847A0 (en) 1991-04-15
IE902289L (en) 1990-12-26
DK0405858T3 (da) 1995-09-04
IE902289A1 (en) 1991-01-16
HUT54415A (en) 1991-02-28
HU903975D0 (en) 1990-11-28
IE67799B1 (en) 1996-05-01
EP0405858B1 (en) 1995-07-19
EP0405858A2 (en) 1991-01-02
US5824496A (en) 1998-10-20
DE69020968T2 (de) 1995-12-21
DE69020968D1 (de) 1995-08-24
JPH0347081A (ja) 1991-02-28
GR3017703T3 (en) 1996-01-31
CA2019696A1 (en) 1990-12-26
ATE125300T1 (de) 1995-08-15
AU5780590A (en) 1991-01-03

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