ES2081119T3 - Sustratos cromogenicos para mejorar la deteccion en un ensayo basado en peroxidasa. - Google Patents

Sustratos cromogenicos para mejorar la deteccion en un ensayo basado en peroxidasa.

Info

Publication number
ES2081119T3
ES2081119T3 ES92915229T ES92915229T ES2081119T3 ES 2081119 T3 ES2081119 T3 ES 2081119T3 ES 92915229 T ES92915229 T ES 92915229T ES 92915229 T ES92915229 T ES 92915229T ES 2081119 T3 ES2081119 T3 ES 2081119T3
Authority
ES
Spain
Prior art keywords
chromogenic substrates
improve detection
based test
peroxidase based
peroxidase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92915229T
Other languages
English (en)
Inventor
Mark Norman Bobrow
Gerald Joseph Litt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Application granted granted Critical
Publication of ES2081119T3 publication Critical patent/ES2081119T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
    • C12Q2326/10—Benzidines
    • C12Q2326/12—3,3',5,5'-Tetramethylbenzidine, i.e. TMB
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
    • C12Q2326/50—Phenols; Naphthols; Catechols
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/815—Test for named compound or class of compounds
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/904—Oxidation - reduction indicators

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

SE DESCRIBEN SUSTRATOS CROMOGENICOS QUE MEJORAN LA DETECCION DE ACTIVIDAD DE PEROXIDOS EN UN INMUNOENSAYO.
ES92915229T 1991-07-12 1992-07-10 Sustratos cromogenicos para mejorar la deteccion en un ensayo basado en peroxidasa. Expired - Lifetime ES2081119T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/729,378 US5238817A (en) 1991-07-12 1991-07-12 Chromogenic substrates for improving detection in a peroxidase-based assay

Publications (1)

Publication Number Publication Date
ES2081119T3 true ES2081119T3 (es) 1996-02-16

Family

ID=24930773

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92915229T Expired - Lifetime ES2081119T3 (es) 1991-07-12 1992-07-10 Sustratos cromogenicos para mejorar la deteccion en un ensayo basado en peroxidasa.

Country Status (10)

Country Link
US (1) US5238817A (es)
EP (1) EP0594709B1 (es)
JP (1) JP3168209B2 (es)
AT (1) ATE130043T1 (es)
AU (1) AU2300292A (es)
DE (1) DE69205982T2 (es)
DK (1) DK0594709T3 (es)
ES (1) ES2081119T3 (es)
GR (1) GR3018770T3 (es)
WO (1) WO1993001307A1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5464755A (en) * 1994-04-29 1995-11-07 Biolog, Inc. Microbiological medium and method of assay
US5925532A (en) * 1995-03-14 1999-07-20 The Trustees Of Columbia University In The City Of New York Enhancing the sensitivity of immunoassay procedures by use of antibodies directed to the product of a reaction between probe labels and assay substrates
US5650284A (en) * 1995-03-14 1997-07-22 The Trustees Of Columbia University In The City Of New York Enhancing the sensitivity of immunoassay procedures by use of antibodies directed to the product of a reaction between probe labels and assay substrates
DE19639538A1 (de) * 1996-09-26 1998-04-09 Boehringer Mannheim Gmbh Enzymatisches Verfahren zum Nachweis der Erhitzungsbehandlung von Milch
US5972595A (en) * 1997-12-19 1999-10-26 Nen Life Science Products, Inc. Enzyme assay using a solid phase substrate
US6066446A (en) * 1997-12-19 2000-05-23 Nen Life Science Products, Inc. Assay member and method for its manufacture
EP3217176B1 (fr) * 2016-03-11 2019-08-07 Scienion AG Procede immunoenzymatique permettant la detection et l'identification sequentielles d'analytes
US11237170B2 (en) 2018-12-04 2022-02-01 Aat Bioquest, Inc. Styryl phenols, derivatives and their use in methods of analyte detection
WO2025230880A1 (en) * 2024-04-29 2025-11-06 Agilent Technologies, Inc. Chromogenic peroxidase substrates

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4503143A (en) * 1982-08-20 1985-03-05 Btc Diagnostics Limited Partnership Enzyme immunoassay with two-part solution of tetramethylbenzidine as chromogen
US4615972A (en) * 1983-11-04 1986-10-07 Immuno Concepts, Inc. Stabilization of indicators for detecting enzyme activity
US4596770A (en) * 1984-06-01 1986-06-24 Travenol-Genetech Diagnostics Assay of peroxidase enzyme activity
EP0196743A3 (en) * 1985-01-31 1988-10-19 Savyon Diagnostics Ltd. Stable chemical compositions containing chromogenic materials and peroxides, and method for obtaining them
IL74205A (en) * 1985-01-31 1990-01-18 Savyon Diagnostics Ltd Method for carrying out enzyme assays utilizing stable chemical compositions containing hydrogen peroxide and a chromogen
JPS628055A (ja) * 1985-07-05 1987-01-16 Shinotesuto Kenkyusho:Kk 酵素免疫測定方法
US4777143A (en) * 1986-12-12 1988-10-11 Litmus Concepts Inc. Method of detecting carboxylic acids in a specimen
IL80964A0 (en) * 1986-12-15 1987-03-31 Savyon Diagnostics Ltd Method for preparing aqueous solutions containing chromogenic materials
JPH0257200A (ja) * 1988-08-22 1990-02-26 Konica Corp パーオキシダーゼ酵素反応を用いた特定成分の測定方法
US5013646A (en) * 1989-11-03 1991-05-07 Transgenic Sciences, Inc. TMB Formulation for soluble and precipitable HRP-ELISA

Also Published As

Publication number Publication date
JPH06508759A (ja) 1994-10-06
ATE130043T1 (de) 1995-11-15
EP0594709B1 (en) 1995-11-08
DE69205982T2 (de) 1996-04-25
WO1993001307A1 (en) 1993-01-21
JP3168209B2 (ja) 2001-05-21
US5238817A (en) 1993-08-24
EP0594709A1 (en) 1994-05-04
AU2300292A (en) 1993-02-11
DE69205982D1 (de) 1995-12-14
GR3018770T3 (en) 1996-04-30
DK0594709T3 (da) 1995-12-11

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