ES2089009T3 - Aparato y metodos para ensayar la susceptibilidad antimicrobiana de microorganismos. - Google Patents
Aparato y metodos para ensayar la susceptibilidad antimicrobiana de microorganismos.Info
- Publication number
- ES2089009T3 ES2089009T3 ES90902964T ES90902964T ES2089009T3 ES 2089009 T3 ES2089009 T3 ES 2089009T3 ES 90902964 T ES90902964 T ES 90902964T ES 90902964 T ES90902964 T ES 90902964T ES 2089009 T3 ES2089009 T3 ES 2089009T3
- Authority
- ES
- Spain
- Prior art keywords
- test
- growth
- well
- volume
- control well
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Rigid containers without fluid transport within
- B01L3/5085—Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Hematology (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Toxicology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
METODO Y APARATO PARA PROBAR LA SUSCEPTIBILIDAD CUALITATIVA DE UN MICROORGANISMO A LA INHIBICION DE CRECIMIENTO POR UN PRODUCTO ANTIMICROBIANO UTILIZANDO UN PANEL DE PRUEBAS (100-1). LOS MODULOS DE PRUEBA (107, 108, 109) ESTAN EN UN POZO DE CONTROL DE CRECIMIENTO NEGATIVA (101), UN POZO DE CONTROL DE CRECIMIENTO POSITIVA (102) Y UN POZO DE PRUEBA (103). LOS MODULOS DE PRUEBA TIENEN UN VOLUMEN SOLIDO SECO DE UN SUBGRUPO DE LOS CONSTITUYENTES DE UN GRUPO DE COMPUESTOS QUIMICOS DE PRUEBA QUE CONTIENEN RESAZURINA Y UN MEDIO DE CRECIMIENTO. EL MODULO DE PRUEBA (109) QUE SE ENCUENTRA EN EL POZO DE PRUEBA TIENE ADEMAS UN VOLUMEN DE PRODUCTO ANTIMICROBIANO. EL MODULO DE PRUEBA (107) QUE SE ENCUENTRA EN EL POZO DE CONTROL DE CRECIMIENTO NEGATIVA SE REHIDRATA CON UN VOLUMEN DE LIQUIDO ESTERIL (110) QUE CONTIENE EL RESTO DE TODOS LOS COMPONENTES QUIMICOS DE PRUEBA QUE NO SE ENCUENTRAN EN EL MODULO DE PRUEBA. LOS MODULOS DE PRUEBA (108, 109) QUE SE ENCUENTRAN EN EL POZO DE CONTROL DE CRECIMIENTO POSITIVA Y EN EL POZO DE PRUEBA SE VUELVEN A HIDRATAR CON UN VOLUMEN DE INOCULO LIQUIDO (112, 113) QUE CONTIENE EL RESTO DE LOS CONSTITUYENTES JUNTO CON UNA CONCENTRACION DE MICROORGANISMOS . EL PANEL DE PRUEBA (100-1) ES INCUBADO Y LEIDO A CONTINUACION UTILIZANDO UN PROTOCOLO DE LECTURA DE LUZ VISIBLE O UN PROTOCOLO DE LECTURA DE EXCITACION FLUORESCENTE PARA DETERMINAR EL CRECIMIENTO O NO CRECIMIENTO QUE TIENE LUGAR EN LOS POZOS. EL PROTOCOLO DE LECTURA DE LUZ VISIBLE DETERMINA EL CRECIMIENTO DEL ORGANISMO ANTE LA PRESENCIA DE UN CAMBIO DE COLOR DE AZUL A ROJO. LOS MODULOS DE PRUEBA SON PREFERIBLEMENTE , DISCOS DE PAPEL CON REACTIVOS DE PRUEBA ABSORBIDOS EN SU INTERIOR Y LA CONCENTRACION DEL PRODUCTO ANTIMICROBIANO IMPRESO SOBRE EL.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US29859989A | 1989-01-17 | 1989-01-17 | |
| US298599 | 1989-01-17 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2089009T3 true ES2089009T3 (es) | 1996-10-01 |
| ES2089009T5 ES2089009T5 (es) | 2008-05-16 |
Family
ID=23151214
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES90902964T Expired - Lifetime ES2089009T5 (es) | 1989-01-17 | 1990-01-16 | Aparato y metodos para ensayar la susceptibilidad antimicrobiana de microorganismos. |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP0454784B2 (es) |
| JP (2) | JPH04502707A (es) |
| AT (1) | ATE136939T1 (es) |
| AU (1) | AU5040990A (es) |
| CA (1) | CA2044265C (es) |
| DE (1) | DE69026602T3 (es) |
| ES (1) | ES2089009T5 (es) |
| WO (1) | WO1990008196A1 (es) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0516814B1 (en) * | 1990-12-28 | 1996-09-04 | Dade MicroScan Inc. | Method and composition for determining antimicrobial susceptibility of the majority of clinically significant gram positive organisms |
| AU4756393A (en) * | 1992-09-30 | 1994-04-14 | Becton Dickinson & Company | Method of critical speciman resistance testing |
| DE4316394C1 (de) * | 1993-05-17 | 1994-06-23 | Biotest Ag | Verfahren zum optischen Nachweis von Mikroorganismen, ihrer Identifizierung und der Empfindlichkeitstestung gegen Antibiotika unter Verwendung eines Redoxindikatorsystems, umfassend Methylenblau und Resazurin |
| US5863751A (en) * | 1996-09-30 | 1999-01-26 | Becton Dickinson And Company | Addition of lactose or other sugars in correcting false susceptibility results for resistant microorganisms in antimicrobial susceptibility tests |
| JP2000116399A (ja) * | 1998-10-14 | 2000-04-25 | Eiken Chem Co Ltd | 薬剤感受性菌・薬剤耐性菌鑑別用分画培地および鑑別方法 |
| WO2000067037A2 (en) * | 1999-04-29 | 2000-11-09 | Dade Microscan Inc. | A combined rapid anti-microbial susceptibility assay and microorganism identification system |
| US6440689B1 (en) * | 1999-12-30 | 2002-08-27 | Ondeo Nalco Company | Measurement of microbiological activity in an opaque medium |
| JP4936637B2 (ja) * | 2001-08-15 | 2012-05-23 | ナルコ カンパニー | 不透明媒質中の微生物活性の測定 |
| EP1891440A4 (en) * | 2005-04-22 | 2009-03-18 | Us Gov Health & Human Serv | MULTIVALENT SELECTION TABLE ARRAYS FOR OPSONOPHAGO CYTOSIS ASSAY AND USES THEREOF |
| US20090142796A1 (en) * | 2007-12-04 | 2009-06-04 | Becton, Dickinson And Company | Detection of Inducible Resistance to Macrolide-Lincosamide-Streptogramin b |
| JP2010057390A (ja) * | 2008-09-02 | 2010-03-18 | Nikken Seibutsu Igaku Kenkyusho:Kk | 検査用具 |
| CN104178550A (zh) * | 2010-06-02 | 2014-12-03 | 湖南省天骑医学新技术有限公司 | 结核分枝杆菌药敏指示剂的使用方法 |
| JP6619544B2 (ja) * | 2014-03-19 | 2019-12-11 | 株式会社日立ハイテクノロジーズ | 検査装置 |
| HK1243740A1 (zh) * | 2014-09-04 | 2018-07-20 | Labrador Diagnostics Llc | 病原体和抗微生物剂抗性检测 |
| EP4303312A3 (en) | 2016-04-22 | 2024-03-13 | Selux Diagnostics, Inc. | Performing antimicrobial susceptibility testing and related systems and methods |
| US12203124B2 (en) * | 2016-12-23 | 2025-01-21 | SeLux Diagnostics, Inc. | Methods for improved rapid antimicrobial susceptibility testing |
| AU2019282414A1 (en) * | 2018-06-08 | 2021-01-07 | SeLux Diagnostics, Inc. | Antimicrobial cartridges and processes for multiplexed antimicrobial susceptibility testing |
| JP6636109B2 (ja) * | 2018-09-11 | 2020-01-29 | 株式会社日立ハイテクノロジーズ | 検査装置 |
| GB201819187D0 (en) * | 2018-11-26 | 2019-01-09 | Univ Manchester | Assay |
| CN115178309B (zh) * | 2021-04-06 | 2024-08-09 | 医流体股份有限公司 | 阵列式微流体芯片及抗生素感受性测试的操作方法 |
| TWI778569B (zh) | 2021-04-06 | 2022-09-21 | 醫流體股份有限公司 | 陣列式微流體晶片及抗生素感受性測試的操作方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3997404A (en) * | 1974-06-07 | 1976-12-14 | Johnston Laboratories, Inc. | Method and apparatus for characterizing biologically active agents |
| US4090920A (en) * | 1976-02-19 | 1978-05-23 | Fisher Scientific Company | Disposable antibiotic susceptability test package |
| US4385115A (en) * | 1980-10-22 | 1983-05-24 | Hoffmann-La Roche Inc. | Diagnostics testing devices and processes |
| US4416995A (en) * | 1981-09-10 | 1983-11-22 | Bronx-Lebanon Hospital Center | Method and apparatus for determining bacterial sensitivity |
-
1990
- 1990-01-16 ES ES90902964T patent/ES2089009T5/es not_active Expired - Lifetime
- 1990-01-16 EP EP90902964A patent/EP0454784B2/en not_active Expired - Lifetime
- 1990-01-16 CA CA002044265A patent/CA2044265C/en not_active Expired - Lifetime
- 1990-01-16 JP JP2503076A patent/JPH04502707A/ja active Pending
- 1990-01-16 AU AU50409/90A patent/AU5040990A/en not_active Abandoned
- 1990-01-16 DE DE69026602T patent/DE69026602T3/de not_active Expired - Lifetime
- 1990-01-16 WO PCT/US1990/000149 patent/WO1990008196A1/en not_active Ceased
- 1990-01-16 AT AT90902964T patent/ATE136939T1/de not_active IP Right Cessation
-
2000
- 2000-07-25 JP JP2000259958A patent/JP3741598B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| DE69026602D1 (de) | 1996-05-23 |
| AU5040990A (en) | 1990-08-13 |
| JPH04502707A (ja) | 1992-05-21 |
| CA2044265A1 (en) | 1990-07-18 |
| EP0454784A4 (en) | 1992-09-09 |
| DE69026602T2 (de) | 1997-01-09 |
| EP0454784A1 (en) | 1991-11-06 |
| EP0454784B1 (en) | 1996-04-17 |
| EP0454784B2 (en) | 2008-01-16 |
| JP3741598B2 (ja) | 2006-02-01 |
| CA2044265C (en) | 2002-03-26 |
| JP2001112498A (ja) | 2001-04-24 |
| ATE136939T1 (de) | 1996-05-15 |
| ES2089009T5 (es) | 2008-05-16 |
| WO1990008196A1 (en) | 1990-07-26 |
| DE69026602T3 (de) | 2008-07-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU5040990A (en) | Apparatus and methods for antimicrobial susceptibility testing of microorganisms | |
| ES2093174T3 (es) | Procedimiento y equipo de ensayo para la deteccion de metabolismo microbiano. | |
| US8632966B2 (en) | Detection of biological molecules by differential partitioning of enzyme substrates and products | |
| Mink et al. | Tentative identification of methanogenic bacteria by fluorescence microscopy | |
| Parkin et al. | The effects of light quality on the growth of phototrophic bacteria in lakes | |
| CA2066329A1 (en) | Microbial monitoring device | |
| ATE83010T1 (de) | Nachweis von mikroben in einer probe. | |
| US4277561A (en) | Support for the determination of enzyme activities and process | |
| IE801417L (en) | Testing for biological tissues | |
| DE68926935D1 (de) | Vorrichtung zur Verstärkung von Fluoreszens und Kinetik und Verfahren zur Anwendung | |
| DE2739421A1 (de) | Diagnostische einrichtung | |
| BR9300639A (pt) | Particulas marcadoras para uso no ensaio de uma amostra de um fluido biologico,processo pra ensaiar uma amostra de fluido biologico,e,processo para ensaiar uma amostra de sangue | |
| CA2264772C (en) | Detection of microorganisms | |
| EP0171158B1 (en) | Processes and materials for carrying out microchemical and microbiological tests | |
| Trainor | Indicator algal assays: laboratory and field approaches | |
| Brodsky et al. | Rapid method for detection of Pseudomonas aeruginosa on MacConkey agar under ultraviolet light | |
| JPH09275998A (ja) | 菌類の即時判別方法及びその判別装置 | |
| EP0653633A3 (en) | Test for the detection of toxicity. | |
| Litchfield et al. | Numerical taxonomy of heterotrophic bacteria growing in association with continuous-culture Chlorella sorokiniana | |
| ES8505404A1 (es) | Metodo y dispositivo para la identificacion de microorganismos en base de pruebas bioquimicas | |
| NO910817D0 (no) | Fremgangsmaate og anordning for studering og kvantifisering av samvirkende effekter paa forbindelser paa biologiske celler. | |
| Maeda et al. | Chromogenic assay method of lipopolysaccharide (LPS) for evaluating bacterial standing crop in seawater | |
| CA1137786A (en) | Automatic scanning apparatus for performing optical density tests on liquid samples, method for testing for antibiotic susceptibility, and method for identifying microorganisms | |
| CN110129403A (zh) | 一种发光细菌毒性检测方法 | |
| WO1995014105A2 (en) | Antibiotic sensitivity profile |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
Ref document number: 454784 Country of ref document: ES |
|
| FG2A | Definitive protection |
Ref document number: 454784 Country of ref document: ES |