ES2091976T3 - Metodos perfeccionados para la amplificacion del acido nucleico. - Google Patents

Metodos perfeccionados para la amplificacion del acido nucleico.

Info

Publication number
ES2091976T3
ES2091976T3 ES92109886T ES92109886T ES2091976T3 ES 2091976 T3 ES2091976 T3 ES 2091976T3 ES 92109886 T ES92109886 T ES 92109886T ES 92109886 T ES92109886 T ES 92109886T ES 2091976 T3 ES2091976 T3 ES 2091976T3
Authority
ES
Spain
Prior art keywords
amplification
methods
primers
nucleic acid
perfected
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92109886T
Other languages
English (en)
Inventor
Henry A Erlich
Russel G Higuchi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Application granted granted Critical
Publication of ES2091976T3 publication Critical patent/ES2091976T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Communicable Diseases (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

SE PRESENTAN METODOS PARA AUMENTAR LA ESPECIFICIDAD Y SENSIBILIDAD DE LA AMPLIFICACION DEL ACIDO NUCLEICO. LOS METODOS SON PROCEDIMIENTOS DE AMPLIFICACION ANIDADOS SIMPLIFICADOS, EN DONDE LOS DOS PARES DE CEBADORES EXTERIOR E INTERIOR SE ENCUENTRAN PRESENTES EN LA MEZCLA DE REACCION DE LA AMPLIFICACION. SEGUN LOS METODOS, EL PERFIL TERMOCICLICO, ASI COMO LAS SECUENCIAS, LONGITUD Y CONCENTRACION DE LOS CEBADORES DE AMPLIFICACION, SE MODIFICAN PARA REGULAR QUE CEBADORES SON RECOCIDOS Y EXTENDIDOS EN EL BLANCO DURANTE UN CICLO DE AMPLIFICACION PARTICULAR. LOS METODOS DESCRITOS SON PARTICULARMENTE UTILES EN AMPLIFICACIONES DE PCR Y PRESENTEN NUMEROSAS APLICACIONES EN LA BIOLOGIA MOLECULAR, LOS DIAGNOSTICOS MEDICOS Y FORENSES.
ES92109886T 1991-06-20 1992-06-12 Metodos perfeccionados para la amplificacion del acido nucleico. Expired - Lifetime ES2091976T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US71857691A 1991-06-20 1991-06-20

Publications (1)

Publication Number Publication Date
ES2091976T3 true ES2091976T3 (es) 1996-11-16

Family

ID=24886600

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92109886T Expired - Lifetime ES2091976T3 (es) 1991-06-20 1992-06-12 Metodos perfeccionados para la amplificacion del acido nucleico.

Country Status (9)

Country Link
US (1) US5314809A (es)
EP (1) EP0519338B1 (es)
JP (1) JPH0681600B2 (es)
CA (1) CA2071594C (es)
DE (1) DE69213112T2 (es)
DK (1) DK0519338T3 (es)
ES (1) ES2091976T3 (es)
GR (1) GR3021832T3 (es)
IE (1) IE922008A1 (es)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2183695A1 (es) * 2000-10-31 2003-03-16 Ivia Metodo y mezcla de reaccion para la amplificacion enzimatica simultanea, coordinada y cooperativa de secuencias diana de acidos nucleicos.

Families Citing this family (167)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5856088A (en) * 1989-07-11 1999-01-05 Gen-Probe Incorporated Detection of human immunodeficiency virus type 1
US6589734B1 (en) 1989-07-11 2003-07-08 Gen-Probe Incorporated Detection of HIV
GB2259138B (en) * 1991-08-27 1995-08-23 Ici Plc Minisatellite variant repeat (MVR-PRC) mapping
US5853989A (en) * 1991-08-27 1998-12-29 Zeneca Limited Method of characterisation of genomic DNA
US5830642A (en) * 1992-04-03 1998-11-03 Amersham Life Science, Inc. Electrophoresis of nucleic acid fragments
US5565339A (en) * 1992-10-08 1996-10-15 Hoffmann-La Roche Inc. Compositions and methods for inhibiting dimerization of primers during storage of polymerase chain reaction reagents
WO1994023069A1 (en) * 1993-03-26 1994-10-13 Gen-Probe Incorporated Detection of human immunodeficiency virus type 1
KR100189229B1 (ko) * 1993-07-23 1999-06-01 다니엘 엘. 캐시앙, 헨리 엘. 노르호프 핵산 증폭을 강화하는 방법
US5556773A (en) * 1993-08-06 1996-09-17 Yourno; Joseph Method and apparatus for nested polymerase chain reaction (PCR) with single closed reaction tubes
US5869255A (en) 1994-02-01 1999-02-09 The Regents Of The University Of California Probes labeled with energy transfer couples dyes exemplified with DNA fragment analysis
US6028190A (en) 1994-02-01 2000-02-22 The Regents Of The University Of California Probes labeled with energy transfer coupled dyes
ATE302208T1 (de) * 1994-02-11 2005-09-15 Qiagen Gmbh Verfahren zur trennung von doppelstrang/einzelstrangnukleinsäurestrukturen
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6395547B1 (en) 1994-02-17 2002-05-28 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5559013A (en) * 1994-06-22 1996-09-24 Johnson & Johnson Clinical Diagnostics, Inc. Method of amplification using intermediate renaturation step
WO1996006190A2 (en) * 1994-08-19 1996-02-29 Perkin-Elmer Corporation Coupled amplification and ligation method
US5871902A (en) * 1994-12-09 1999-02-16 The Gene Pool, Inc. Sequence-specific detection of nucleic acid hybrids using a DNA-binding molecule or assembly capable of discriminating perfect hybrids from non-perfect hybrids
US20030104361A1 (en) 1997-09-29 2003-06-05 Susan Weininger Method of detection of nucleic acids with a specific sequence composition
ATE196322T1 (de) * 1995-05-05 2000-09-15 Perkin Elmer Corp Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay
JPH11505126A (ja) * 1995-05-19 1999-05-18 アボツト・ラボラトリーズ プライマーシリーズ集団を使用する広い動的範囲の核酸検出
US5882857A (en) * 1995-06-07 1999-03-16 Behringwerke Ag Internal positive controls for nucleic acid amplification
AT402203B (de) 1995-06-13 1997-03-25 Himmler Gottfried Dipl Ing Dr Verfahren zur transkriptionsfreien amplifizierung von nucleinsäuren
US6852487B1 (en) * 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US20020150921A1 (en) * 1996-02-09 2002-10-17 Francis Barany Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6506602B1 (en) 1996-03-25 2003-01-14 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
JP4468488B2 (ja) * 1996-05-29 2010-05-26 コーネル・リサーチ・ファンデーション・インコーポレイテッド 組み合せたリガーゼ検出およびポリメラーゼ連鎖反応を用いる核酸配列相違の検出
US20070009930A1 (en) * 1996-12-18 2007-01-11 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6333179B1 (en) 1997-06-20 2001-12-25 Affymetrix, Inc. Methods and compositions for multiplex amplification of nucleic acids
US6207425B1 (en) * 1997-09-11 2001-03-27 City Of Hope Bidirectional PCR amplification of specific alleles
US6103468A (en) * 1997-10-07 2000-08-15 Labatt Brewing Company Limited Rapid two-stage polymerase chain reaction method for detection of lactic acid bacteria in beer
EP1036198B1 (en) * 1997-12-08 2012-09-26 California Institute Of Technology Method for creating polynucleotide and polypeptide sequences
WO1999034014A2 (en) * 1997-12-23 1999-07-08 Roche Diagnostics Gmbh A method for the determination of a nucleic acid
JP3784162B2 (ja) * 1998-01-14 2006-06-07 株式会社日立製作所 Ｄｎａ断片分析法
US6365408B1 (en) 1998-06-19 2002-04-02 Maxygen, Inc. Methods of evolving a polynucleotides by mutagenesis and recombination
US6506594B1 (en) * 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US7014994B1 (en) 1999-03-19 2006-03-21 Cornell Research Foundation,Inc. Coupled polymerase chain reaction-restriction-endonuclease digestion-ligase detection reaction process
IL145677A0 (en) * 1999-04-12 2002-06-30 Genentech Inc Pcr-based cloning method
US6232104B1 (en) * 1999-08-17 2001-05-15 Dade Behring Inc. Detection of differences in nucleic acids by inhibition of spontaneous DNA branch migration
US7585632B2 (en) * 1999-10-29 2009-09-08 Hologic, Inc. Compositions and methods for the detection of a nucleic acid using a cleavage reaction
US8076063B2 (en) * 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
ATE411397T1 (de) * 2000-02-07 2008-10-15 Illumina Inc Nukleinsäure-nachweisverfahren mit universellem priming
US7955794B2 (en) * 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
CA2405412A1 (en) 2000-04-14 2001-10-25 Cornell Research Foundation, Inc. Method of designing addressable array for detection of nucleic acid sequence differences using ligase detection reaction
US6887664B2 (en) * 2000-06-06 2005-05-03 Applera Corporation Asynchronous primed PCR
EP1176212A1 (en) * 2000-07-24 2002-01-30 Centre National de Genotype Method for haplotyping by mass spectrometry
CA2421102A1 (en) 2000-08-25 2002-03-07 Lovelace Respiratory Research Institute Nested methylation-specific polymerase chain reaction cancer detection method
EP1186669B1 (de) * 2000-09-05 2006-01-25 Zeltz, Patrick, Dr. Verfahren zur spezifischen Bestimmung von DNA-Sequenzen mittels paralleler Amplifikation
IL155450A0 (en) * 2000-10-20 2003-11-23 Expression Diagnostics Inc Leukocyte expression profiling
AU2007221860B2 (en) * 2001-03-02 2012-03-15 University Of Pittsburgh Of The Commonwealth System Of Higher Education PCR method
CA2439402A1 (en) * 2001-03-02 2002-09-12 University Of Pittsburgh Of The Commonwealth System Of Higher Education Pcr method
US20060068433A1 (en) * 2004-09-20 2006-03-30 Godfrey Tony E Multiple mode multiplex reaction quenching method
ES2189631B1 (es) * 2001-03-12 2005-01-01 BIOTOOLS BIOTECHNOLOGICAL & MEDICAL LABORATORIES, S.A. Metodo para la amplificacion de adn mediante semi-nested pcr en un solo paso y su empleo en la identificacion de especies de plasmodium.
US6905827B2 (en) * 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
US7026121B1 (en) 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7235358B2 (en) * 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
DE60324253D1 (de) * 2002-02-08 2008-12-04 Olympus Corp Spezifische multiplex-analyse von nukleinsäuren
WO2003072054A2 (en) * 2002-02-25 2003-09-04 Cabot Corporation Custom ligand design for biomolecular filtration and purification for bioseperation
US7179639B2 (en) * 2002-03-05 2007-02-20 Raveendran Pottathil Thermal strip thermocycler
WO2003100019A2 (en) * 2002-05-24 2003-12-04 Invitrogen Corporation Nested pcr employing degradable primers
US20040259105A1 (en) * 2002-10-03 2004-12-23 Jian-Bing Fan Multiplex nucleic acid analysis using archived or fixed samples
TW200413527A (en) 2002-10-29 2004-08-01 Riken Amplification of nucleic acid
EP1587940A4 (en) * 2002-12-20 2006-06-07 Caliper Life Sciences Inc SINGLE MOLECULAR AMPLIFICATION AND DNA PROOF
US7820030B2 (en) * 2003-04-16 2010-10-26 Handylab, Inc. System and method for electrochemical detection of biological compounds
US7892745B2 (en) * 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
EP1687437A4 (en) * 2003-10-13 2008-01-02 Genaco Biomedial Products Inc METHOD AND KIT FOR MULTIPLEX AMPLIFICATION OF NUCLEIC ACID PRIMERS
US7824857B2 (en) * 2003-12-02 2010-11-02 Musc Foundation For Research Development Methods and compositions for diagnosing epithelial cell cancer
JP4395363B2 (ja) * 2003-12-16 2010-01-06 日立ソフトウエアエンジニアリング株式会社 核酸検出方法
US8206902B2 (en) 2003-12-25 2012-06-26 Riken Method of amplifying nucleic acid and method of detecting mutated nucleic acid using the same
US20060141487A1 (en) * 2004-01-26 2006-06-29 Applera Corporation Methods, compositions, and kits for amplifying and sequencing polynucleotides
US7981616B2 (en) * 2004-02-27 2011-07-19 Musc Foundation For Research Development Enhanced detection of RNA using a panel of truncated gene-specific primers for reverse transcription
US7695941B2 (en) * 2005-06-16 2010-04-13 The United States Of America As Represented By The Secretary Of The Navy Multiplexed polymerase chain reaction for genetic sequence analysis
WO2006112818A2 (en) 2005-04-14 2006-10-26 Applera Corporation 3' modified oligonucleotides containing pseudoisocytosine nucleobase derivatives and applications thereof as primers or probes
WO2006017150A2 (en) * 2004-07-09 2006-02-16 University Of Pittsburgh - Of The Commonwealth System Of Higher Educatiion Identification of markers in lung and breast cancer
WO2006029184A2 (en) * 2004-09-08 2006-03-16 Expression Diagnostics, Inc. Genes useful for diagnosing and monitoring inflammation related disorders
US20060188910A1 (en) * 2005-02-18 2006-08-24 Applera Corporation Compositions, methods, and kits for analyzing DNA methylation
WO2006122295A2 (en) * 2005-05-11 2006-11-16 Expression Diagnostics, Inc. Methods of monitoring functional status of transplants using gene panels
US20070048758A1 (en) * 2005-06-09 2007-03-01 Epoch Biosciences, Inc. Improved primer-based amplification methods
US11111543B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
JP5025489B2 (ja) * 2005-12-28 2012-09-12 学校法人麻布獣医学園 標的配列の特異的高感度増幅法
CA2648580A1 (en) * 2006-04-07 2008-05-02 Xdx, Inc. Steroid responsive nucleic acid expression and prediction of disease activity
US8232091B2 (en) * 2006-05-17 2012-07-31 California Institute Of Technology Thermal cycling system
US8119352B2 (en) * 2006-06-20 2012-02-21 Cepheld Multi-stage amplification reactions by control of sequence replication times
US7993832B2 (en) * 2006-08-14 2011-08-09 Xdx, Inc. Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders
US20080311628A1 (en) * 2006-10-03 2008-12-18 Ghc Technologies, Inc. Methods and compositions for rapid amplification and capture of nucleic acid sequences
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
EP2102367A2 (en) * 2006-11-09 2009-09-23 XDX, Inc. Methods for diagnosing and monitoring the status of systemic lupus erythematosus
US9938641B2 (en) * 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
ES2725003T3 (es) * 2007-03-28 2019-09-18 Signal Diagnostics Sistema y método de análisis de alta resolución de ácidos nucleicos para detectar variaciones de secuencia
US20100273159A1 (en) * 2007-06-20 2010-10-28 Taag-Genetics S.A. Nested Multiplex Amplification Method for Identification of Multiple Biological Entities
WO2009036514A2 (en) * 2007-09-20 2009-03-26 Molecular Plant Breeding Nominees Ltd Method of amplifying nucleic acid
JP2009178159A (ja) * 2007-11-05 2009-08-13 Hitachi Plant Technologies Ltd 核酸配列の検出方法及び核酸配列検出基板
EP2240613B1 (en) 2008-02-06 2013-09-11 Ludwig-Maximilians-Universität München Thermo-optical characterisation of nucleic acid molecules
EP2163239A1 (de) 2008-05-27 2010-03-17 Qiagen GmbH Produkte, die Biopartikel enthalten, Verfahren zu ihrer Herstellung
BRPI0913658B1 (pt) 2008-07-02 2019-06-25 Nipro Corporation Método, tira de teste e kit para detecção da sensibilidade à isoniazida em mycobacterium tuberculosis
EP2295600A4 (en) 2008-07-02 2012-01-04 Nipro Corp METHOD AND TEST STRIP FOR THE DETECTION OF SENSITIVITY AGAINST ISONIAZIDE IN MYCOBACTERIUM TUBERCULOSIS
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US9194861B2 (en) * 2009-09-02 2015-11-24 Bio-Rad Laboratories, Inc. Method of mixing fluids by coalescence of multiple emulsions
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9399215B2 (en) 2012-04-13 2016-07-26 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US12162008B2 (en) 2008-09-23 2024-12-10 Bio-Rad Laboratories, Inc. Partition-based method of analysis
WO2011120020A1 (en) 2010-03-25 2011-09-29 Quantalife, Inc. Droplet transport system for detection
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US12090480B2 (en) 2008-09-23 2024-09-17 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US8633015B2 (en) * 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
US9598725B2 (en) 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
CN102405402A (zh) 2008-09-23 2012-04-04 阔达生命有限公司 基于液滴的测定系统
US10669574B2 (en) 2008-11-18 2020-06-02 XCR Diagnostics, Inc. DNA amplification technology
CN102282257B (zh) 2009-01-15 2015-04-01 北海道三井化学株式会社 含有耐热性dna聚合酶的酶制品及其制造方法、以及检测对象生物的检测方法
WO2010104798A1 (en) * 2009-03-08 2010-09-16 Ibis Biosciences, Inc. Bioagent detection methods
CN202830041U (zh) * 2009-04-03 2013-03-27 Illumina公司 用于加热生物样本的设备
JP2012528574A (ja) * 2009-06-02 2012-11-15 モノクアント・ピーティーワイ・リミテッド 核酸増幅の方法
EP2264184A1 (de) 2009-06-22 2010-12-22 Qiagen GmbH Verfahren zur Amplifikation von DNA unter Verwendung von Tetraethylenglykol, Kit-of-parts dafür und dessen Verwendung
US8987685B2 (en) * 2009-09-09 2015-03-24 Pcr Max Limited Optical system for multiple reactions
CA2777446A1 (en) * 2009-10-13 2011-04-21 Syntezza Molecular Detection Israel Ltd. Methods and compositions for amplifying target sequences from nucleic acid samples
US8771951B2 (en) * 2009-11-16 2014-07-08 Genomics Usa, Inc. Methods for PCR and HLA typing using raw blood
US9416419B2 (en) 2009-11-16 2016-08-16 Michael E. Hogan Methods for PCR and HLA typing using unpurified samples
EP2550528B1 (en) 2010-03-25 2019-09-11 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
CA2767113A1 (en) 2010-03-25 2011-09-29 Bio-Rad Laboratories, Inc. Detection system for droplet-based assays
CN102985560A (zh) * 2010-03-30 2013-03-20 莫诺匡特私人有限公司 调控寡核苷酸功能性的方法
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US12152275B2 (en) * 2010-05-18 2024-11-26 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
CA2816712C (en) 2010-11-01 2018-12-11 Donald A. Masquelier System for forming emulsions
CA2824387C (en) 2011-02-09 2019-09-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US12097495B2 (en) 2011-02-18 2024-09-24 Bio-Rad Laboratories, Inc. Methods and compositions for detecting genetic material
JP2014509865A (ja) 2011-03-18 2014-04-24 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド シグナルの組合せ使用による多重化デジタルアッセイ
EP2702175B1 (en) 2011-04-25 2018-08-08 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
WO2013019751A1 (en) 2011-07-29 2013-02-07 Bio-Rad Laboratories, Inc., Library characterization by digital assay
BR112014019168B1 (pt) 2012-02-03 2021-10-05 California Institute Of Technology Método capaz de detectar de forma não degenerada presença ou ausência de analitos em um único volume de amostra e método de detecção da presença ou ausência de cada analito dentre uma pluralidade de analitos
CN104662172B (zh) 2012-08-03 2018-07-06 加州理工学院 Pcr中具有减少的硬件和要求的多重化和定量
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
US10370707B2 (en) 2013-10-09 2019-08-06 Fluoresentric, Inc. Multiplex probes
US20180173846A1 (en) 2014-06-05 2018-06-21 Natera, Inc. Systems and Methods for Detection of Aneuploidy
SG11201707154SA (en) * 2015-03-06 2017-09-28 Pillar Biosciences Inc Selective amplification of overlapping amplicons
US10221448B2 (en) 2015-03-06 2019-03-05 Pillar Biosciences Inc. Selective amplification of overlapping amplicons
DK3294906T3 (en) 2015-05-11 2024-08-05 Natera Inc Methods for determining ploidy
WO2017181202A2 (en) 2016-04-15 2017-10-19 Natera, Inc. Methods for lung cancer detection
US10066263B2 (en) * 2016-06-17 2018-09-04 California Institute Of Technology Nucleic acid reactions and related methods and compositions
GB201618485D0 (en) 2016-11-02 2016-12-14 Ucl Business Plc Method of detecting tumour recurrence
US20210079456A1 (en) * 2017-07-26 2021-03-18 Sekisui Medical Co., Ltd. Method for detecting mutant gene
CA3085933A1 (en) 2017-12-14 2019-06-20 Tai Diagnostics, Inc. Assessing graft suitability for transplantation
WO2019161244A1 (en) 2018-02-15 2019-08-22 Natera, Inc. Methods for isolating nucleic acids with size selection
EP3781714B1 (en) 2018-04-14 2026-01-07 Natera, Inc. Methods for cancer detection and monitoring by means of personalized detection of circulating tumor dna
CN112424377A (zh) 2018-04-17 2021-02-26 克罗玛科德公司 用于多重分析的方法和系统
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
US12234509B2 (en) 2018-07-03 2025-02-25 Natera, Inc. Methods for detection of donor-derived cell-free DNA
EP4087941A4 (en) * 2020-01-10 2023-06-28 Siemens Healthcare Diagnostics, Inc. Compositions, kits, and methods for performing rapid polymerase chain reactions
US20230366038A1 (en) * 2022-05-16 2023-11-16 Bioo Scientific Corporation Compositions and methods relating to loop mediated isothermal amplification (lamp)
CN116959580B (zh) * 2023-08-30 2025-09-19 予果生物科技（北京）有限公司 一种基于靶向高通量测序序列的比对方法及其应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4358535A (en) 1980-12-08 1982-11-09 Board Of Regents Of The University Of Washington Specific DNA probes in diagnostic microbiology
US4582789A (en) * 1984-03-21 1986-04-15 Cetus Corporation Process for labeling nucleic acids using psoralen derivatives
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
CA1339653C (en) 1986-02-25 1998-02-03 Larry J. Johnson Appartus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
CA1284931C (en) 1986-03-13 1991-06-18 Henry A. Erlich Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
WO1989009835A1 (en) 1988-04-08 1989-10-19 The Salk Institute For Biological Studies Ligase-based amplification method

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2183695A1 (es) * 2000-10-31 2003-03-16 Ivia Metodo y mezcla de reaccion para la amplificacion enzimatica simultanea, coordinada y cooperativa de secuencias diana de acidos nucleicos.

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DE69213112T2 (de) 1997-04-03
CA2071594A1 (en) 1992-12-21
US5314809A (en) 1994-05-24
IE922008A1 (en) 1992-12-30
EP0519338B1 (en) 1996-08-28
GR3021832T3 (en) 1997-02-28
DE69213112D1 (de) 1996-10-02
JPH05292968A (ja) 1993-11-09
CA2071594C (en) 2003-06-17
DK0519338T3 (da) 1996-10-28
JPH0681600B2 (ja) 1994-10-19
EP0519338A1 (en) 1992-12-23

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