ES2093555A1 - Procedimiento de preparacion de derivados de d-amino acido oxidasas y glutaril acilasas altamente estabilizados para su uso como catalizadores para la produccion de acido 7-amino cefalosporanico (7-aca) a partir de cefalosporina c. - Google Patents

Procedimiento de preparacion de derivados de d-amino acido oxidasas y glutaril acilasas altamente estabilizados para su uso como catalizadores para la produccion de acido 7-amino cefalosporanico (7-aca) a partir de cefalosporina c.

Info

Publication number
ES2093555A1
ES2093555A1 ES09500369A ES9500369A ES2093555A1 ES 2093555 A1 ES2093555 A1 ES 2093555A1 ES 09500369 A ES09500369 A ES 09500369A ES 9500369 A ES9500369 A ES 9500369A ES 2093555 A1 ES2093555 A1 ES 2093555A1
Authority
ES
Spain
Prior art keywords
production
amino
enzyme
cephalosporin
aca
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES09500369A
Other languages
English (en)
Other versions
ES2093555B1 (es
Inventor
Seijas Jose Manuel Guisan
Rodriguez Veronica Rodriguez
Lafuente Roberto Fernandez
Codina M Agatha Bastida
Rodrguez M Del Carmen Ceinos
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Antibioticos SA
Original Assignee
Antibioticos SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Antibioticos SA filed Critical Antibioticos SA
Priority to ES09500369A priority Critical patent/ES2093555B1/es
Publication of ES2093555A1 publication Critical patent/ES2093555A1/es
Application granted granted Critical
Publication of ES2093555B1 publication Critical patent/ES2093555B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cephalosporin Compounds (AREA)

Abstract

PROCEDIMIENTO DE PREPARACION DE DERIVADOS DE D-AMINO ACIDO OXIDASAS Y GLUTARIL ACILASAS ALTAMENTE ESTABILIZADOS PARA SU USO COMO CATALIZADORES PARA LA PRODUCCION DE ACIDO 7-AMINO CEFALOSPORANICO (7-ACA) A PARTIR DE CEFALOSPORINA C. ESTE PROCEDIMIENTO ESTA BASADO EN EL DESARROLLO DE NUEVAS ESTRATEGIAS DE INMOVILIZACION DE ENZIMAS. DE ESTE MODO LOGRAMOS CONTROLAR LA ORIENTACION CON LA QUE LA ENZIMA SE INMOVILIZA SOBRE EL SOPORTE, LOGRAMOS CONTROLAR EL GRADO DE INMOVILIZACION MULTIPUNTUAL Y LOGRAMOS DESARROLLAR MODIFICACIONES QUIMICAS ADICIONALES DE LAS MOLECULAS DE ENZIMA YA INMOVILIZADAS. LOS DERIVADOS ENZIMATICOS ASI CONSEGUIDOS ERAN MUCHO MAS ESTABLES QUE LAS CORRESPONDIENTES ENZIMAS NATIVAS FRENTE A LOS EFECTOS INACTIVANTES DEL CALOR, PH DEL MEDIO Y DE LA PRESENCIA DE CONCENTRACIONES MODERADAS DE PEROXIDO DE HIDROGENO. DE ESTE MODO ESTA ESTABILIDAD MEJORADA DE ESTAS ENZIMAS NOS PERMITE UTILIZAR ESTOS DERIVADOS ENZIMATICOS PARA OPTIMIZAR EL DESARROLLO DE UN PROCESO DE ENORME INTERES EN LAINDUSTRIA FARMACEUTICA: LA OBTENCION DE 7-ACA, INTERMEDIO CLAVE PARA LA OBTENCION DE CEFALOSPORINAS SEMISINTETICAS.
ES09500369A 1995-02-24 1995-02-24 Procedimiento de preparacion de derivados de d-amino acido oxidasas y glutaril acilasas altamente estabilizados para su uso como catalizadores para la produccion de acido 7-amino cefalosporanico (7-aca) a partir de cefalosporina c. Expired - Fee Related ES2093555B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES09500369A ES2093555B1 (es) 1995-02-24 1995-02-24 Procedimiento de preparacion de derivados de d-amino acido oxidasas y glutaril acilasas altamente estabilizados para su uso como catalizadores para la produccion de acido 7-amino cefalosporanico (7-aca) a partir de cefalosporina c.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES09500369A ES2093555B1 (es) 1995-02-24 1995-02-24 Procedimiento de preparacion de derivados de d-amino acido oxidasas y glutaril acilasas altamente estabilizados para su uso como catalizadores para la produccion de acido 7-amino cefalosporanico (7-aca) a partir de cefalosporina c.

Publications (2)

Publication Number Publication Date
ES2093555A1 true ES2093555A1 (es) 1996-12-16
ES2093555B1 ES2093555B1 (es) 1997-07-01

Family

ID=8289576

Family Applications (1)

Application Number Title Priority Date Filing Date
ES09500369A Expired - Fee Related ES2093555B1 (es) 1995-02-24 1995-02-24 Procedimiento de preparacion de derivados de d-amino acido oxidasas y glutaril acilasas altamente estabilizados para su uso como catalizadores para la produccion de acido 7-amino cefalosporanico (7-aca) a partir de cefalosporina c.

Country Status (1)

Country Link
ES (1) ES2093555B1 (es)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000073490A1 (de) * 1999-05-28 2000-12-07 Aventis Pharma Deutschland Gmbh Verfahren zur verzögerung der desaktivierung von glutarylamidase während einer enzymkatalyse

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0496993A1 (en) * 1990-12-21 1992-08-05 ANTIBIOTICOS, S.p.A. Enzymatic process for preparing 7-aminocephalosporanic acid and derivatives

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0496993A1 (en) * 1990-12-21 1992-08-05 ANTIBIOTICOS, S.p.A. Enzymatic process for preparing 7-aminocephalosporanic acid and derivatives

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NIKOLOV, A. et al., "Enzymatic transformation of cephalosporin C to 7-amino-cephalosporanic acid. Part II: single-step procedure using a coimmobilized enzyme system", diciembre 1994, Enzyme Microb. Technol., vol. 16, pp.: 1037-1041. * todo el documento * *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000073490A1 (de) * 1999-05-28 2000-12-07 Aventis Pharma Deutschland Gmbh Verfahren zur verzögerung der desaktivierung von glutarylamidase während einer enzymkatalyse

Also Published As

Publication number Publication date
ES2093555B1 (es) 1997-07-01

Similar Documents

Publication Publication Date Title
Terreni et al. Modulation of penicillin acylase properties via immobilization techniques: one-pot chemoenzymatic synthesis of cephamandole from cephalosporin C
Dosanjh et al. Immobilization, stability and esterification studies of a lipase from a Bacillus sp.
Mateo et al. Epoxy sepabeads: a novel epoxy support for stabilization of industrial enzymes via very intense multipoint covalent attachment
Fernández-Lafuente et al. The presence of methanol exerts a strong and complex modulation of the synthesis of different antibiotics by immobilized penicillin G acylase
López-Gallego et al. Preparation of a robust biocatalyst of D-amino acid oxidase on sepabeads supports using the glutaraldehyde crosslinking method
Fernández-Lafuente et al. Biotransformations catalyzed by multimeric enzymes: stabilization of tetrameric ampicillin acylase permits the optimization of ampicillin synthesis under dissociation conditions
KR920012454A (ko) 7-아미노세팔로스포란 산 및 이의 유도체를 제조하는 효소적방법
Fernández-Lorente et al. Immobilization of proteins on glyoxyl activated supports: dramatic stabilization of enzymes by multipoint covalent attachment on pre-existing supports
KR920701468A (ko) 7-아미노 세팔로스포란산의 효소적 제조방법
López-Gallego et al. Optimization of an industrial biocatalyst of glutaryl acylase: stabilization of the enzyme by multipoint covalent attachment onto new amino-epoxy Sepabeads
Ma et al. One-pot enzymatic production of deacetyl-7-aminocephalosporanic acid from cephalosporin C via immobilized cephalosporin C acylase and deacetylase
Monti et al. Characterization of an industrial biocatalyst: Immobilized glutaryl‐7‐ACA acylase
Wei et al. Effects of ethylene glycol on the synthesis of ampicillin using immobilized penicillin G acylase
Aramori et al. Comparative characterization of new glutaryl 7-ACA and cephalosporin C acylases
Golini et al. Immobilization of D-amino acid oxidase from different yeasts: characterization and application in the deamination of cephalosporin C
Alvaro et al. Penicillin G acylase from Kluyvera citrophila new choice as industrial enzyme
Torres‐Bacete et al. Stabilization of penicillin V acylase from Streptomyces lavendulae by covalent immobilization
MX9803762A (es) Produccion microbiana mejorada de indigo.
US5268271A (en) Method for the synthesis of semi-synthetic antibiotics in thermodynamically controlled water-cosolvent organic miscible apolar systems by using penicillin G acylase
Johnson et al. [53f] β-Lactamases (Actinomycetes species)
Kazan et al. Stabilization of penicillin G acylase against pH by chemical cross-linking
Serra et al. Coupling of site‐directed mutagenesis and immobilization for the rational design of more efficient biocatalysts: the case of immobilized 3G3K PGA from E. coli
EP1344830A1 (en) A process for the preparation of cephalosporan acid derivatives from cephalosporin C
AU4846196A (en) Process for the recovery of cephalexin
JPS59210891A (ja) 酵素を用いる方法

Legal Events

Date Code Title Description
EC2A Search report published

Date of ref document: 19961216

Kind code of ref document: A1

Effective date: 19961216

FD2A Announcement of lapse in spain

Effective date: 20180806