ES2096041T3 - Deteccion de secuencias nucleotidicas complementarias. - Google Patents

Deteccion de secuencias nucleotidicas complementarias.

Info

Publication number
ES2096041T3
ES2096041T3 ES92307441T ES92307441T ES2096041T3 ES 2096041 T3 ES2096041 T3 ES 2096041T3 ES 92307441 T ES92307441 T ES 92307441T ES 92307441 T ES92307441 T ES 92307441T ES 2096041 T3 ES2096041 T3 ES 2096041T3
Authority
ES
Spain
Prior art keywords
enzyme
sequence
nucleic acid
specific
probe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92307441T
Other languages
English (en)
Inventor
Scott J Eisenbeis
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Microgenics Corp
Original Assignee
Microgenics Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Microgenics Corp filed Critical Microgenics Corp
Application granted granted Critical
Publication of ES2096041T3 publication Critical patent/ES2096041T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

EL INVENTO MUESTRA UN METODO PARA LA DETECCION DE UN ACIDO NUCLEICO ESPECIFICO QUE COMPRENDE FORMAR UNA MEZCLA DE REACCION COMBINANDO (1) UNA MUESTRA QUE SE SOSPECHA CONTIENE UN ACIDO NUCLEICO (2) UN CONJUGADO POLIPEPTIDO DONANTE DE ENZIMA/SONDA QUE COMPRENDE (A) UNA SECUENCIA DE CONJUGADO POLIPEPTIDO DONANTE DE ENZIMA QUE COMPRENDE UN FRAGMENTO DE (BETA) ASA; Y (B) UNA SECUENCIA DE OLIGONUCLEOTIDO DE UNA SOLA FILA FIJADO A (A) Y CAPAZ DE HIBRIDIZAR CON DICHO ACIDO NUCLEICO; (3) UN POLIPEPTIDO ACEPTADOR DE ENZIMA CAPAZ DE FORMAR UNA ENZIMA ACTIVA EN COMPLEMENTACION CON DICHO FRAGMENTO DE DONANTE DE ENZIMA; Y (4) UN SUSTRATO PARA (BETA) HIBRIDACION DE DICHO CONJUGADO DONANTE DE ENZIMA/SONDA A DICHO ACIDO NUCLEICO DE MUESTRA PARA FORMAR UNA SECUENCIA ESPECIFICA DE DOBLE FILA DETERMINANDO LA CANTIDAD O FRECUENCIA DE ACTIVIDAD ENZIMATICA EN DICHO SUSTRATO EN DICHA MEZCLA DE REACCION. EL METODO PUEDE INCLUIR ASIMISMO UNA FUNCION DE "LECTURA DE PRUEBA" INCUBANDO LA SONDA HIBRIDIZADA CON AL MENOSUNA ENDONUCLEASA DE RESTRICCION ESPECIFICA DE SECUENCIA ESPECIFICA DE DOBLE FILA. SE INCLUYEN EQUIPOS NUEVOS PARA UTILIZAR EN LA ELABORACION DE ESTE METODO.
ES92307441T 1991-08-15 1992-08-13 Deteccion de secuencias nucleotidicas complementarias. Expired - Lifetime ES2096041T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US74515391A 1991-08-15 1991-08-15

Publications (1)

Publication Number Publication Date
ES2096041T3 true ES2096041T3 (es) 1997-03-01

Family

ID=24995480

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92307441T Expired - Lifetime ES2096041T3 (es) 1991-08-15 1992-08-13 Deteccion de secuencias nucleotidicas complementarias.

Country Status (8)

Country Link
US (1) US5795718A (es)
EP (1) EP0530998B1 (es)
JP (1) JP2761159B2 (es)
AT (1) ATE145250T1 (es)
AU (1) AU653244B2 (es)
CA (1) CA2075858C (es)
DE (1) DE69215169T2 (es)
ES (1) ES2096041T3 (es)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2863638B2 (ja) * 1996-01-26 1999-03-03 ベーリンガー マンハイム コーポレイション ビスーマレイミド架橋剤
US5763196A (en) * 1996-01-26 1998-06-09 Boehringer Mannheim Corporation Assays using cross-linked polypeptide fragments of β-galactosidase
US5976857A (en) * 1996-01-26 1999-11-02 Boehringer Mannheim Corporation Cross-linked polypeptide fragments of β-galactosidase
EP0933431A1 (en) * 1996-07-11 1999-08-04 Wakunaga Pharmaceutical Co., Ltd. Method for examining nucleic acids and examination kits
DE19749163A1 (de) * 1997-11-07 1999-05-12 Roche Diagnostics Gmbh Verfahren und Nachweis von Nukleinsäuren
AU4329001A (en) * 2000-02-24 2001-09-03 Compound Therapeutics, Inc. Improved methods for generating catalytic proteins
DE10041766A1 (de) * 2000-08-25 2002-03-14 Friz Biochem Gmbh Verfahren zur Markierung chemischer Substanzen
US8182986B1 (en) * 2001-07-12 2012-05-22 Discoverx Assays for nucleic acid modifying enzymes
US7662554B2 (en) * 2002-10-09 2010-02-16 The Trustees Of Boston University Nucleic acid supported protein complementation
EP1611254B1 (en) 2003-03-31 2014-09-10 Roche Diagnostics GmbH Compositions and methods for detecting certain flaviviruses, including members of the japanese encephalitis virus serogroup
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
AU2006305901A1 (en) * 2005-10-27 2007-05-03 Trustees Of Boston University Real time nucleic acid detection in vivo using protein complementation
KR20080070838A (ko) * 2005-10-27 2008-07-31 트러스티스 오브 보스턴 유니버시티 활성화된 분절­폴리펩티드 및 이의 생산 방법 및 이의 용도
RU2009120007A (ru) * 2006-10-27 2010-12-10 Трастиз Оф Бостон Юниверсити (Us) Направленные конъюгаты с разделенными биомолекулами для лечения заболеваний, злокачественных образований и нарушений и способы их получения
EP2318549B1 (en) 2008-08-15 2013-06-26 Cascade Biosystems, Inc. Detecting nucleic acid
US8597886B2 (en) 2010-02-15 2013-12-03 Cascade Biosystems, Inc. Methods and materials for detecting viral or microbial infections
CA2789874C (en) 2010-02-15 2020-03-24 Cascade Biosystems, Inc. Methods and materials for assessing rna expression
CA2790006C (en) 2010-02-15 2019-09-24 Cascade Biosystems, Inc. Enzymatic amplification methods and materials for detecting contaminatedfood products
EP2536848B1 (en) 2010-02-15 2017-07-19 Cascade Biosystems, Inc. Methods and materials for detecting genetic or epigenetic elements
GB2643378A (en) * 2024-07-15 2026-02-18 Biocrucible Ltd Reagents and methods

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0144914A3 (en) * 1983-12-12 1986-08-13 Miles Inc. Hybridization assay employing labeled pairs of hybrid binding reagents
EP0144913A3 (en) * 1983-12-12 1986-08-20 Miles Inc. Hybridization assay employing labeled probe and anti-hybrid
US4699876A (en) * 1984-01-27 1987-10-13 E. I. Du Pont De Nemours And Company Nonradiometric polynucleotide probes
US4708929A (en) * 1984-10-29 1987-11-24 Microgenics Corporation Methods for protein binding enzyme complementation assays
US4683194A (en) * 1984-05-29 1987-07-28 Cetus Corporation Method for detection of polymorphic restriction sites and nucleic acid sequences
US4775619A (en) * 1984-10-16 1988-10-04 Chiron Corporation Polynucleotide determination with selectable cleavage sites
JPH0779717B2 (ja) * 1984-10-29 1995-08-30 マイクロジエニツクス コ−ポレ−シヨン タンパク質結合酵素相補性検定に関する方法
US4868103A (en) * 1986-02-19 1989-09-19 Enzo Biochem, Inc. Analyte detection by means of energy transfer
DE69028104T2 (de) * 1989-05-05 1997-02-06 Microgenics Corp Verfahren zur proteinbindung-enzym-komplementationstests
US5082780A (en) * 1989-09-12 1992-01-21 Eastman Kodak Company Oligonucleotide-enzyme conjugate that can be used as a probe in hybridization assays and polymerase chain reaction procedures
EP0419081A3 (en) * 1989-09-22 1992-07-22 Microgenics Corporation Method for protein binding enzyme complementation assays

Also Published As

Publication number Publication date
JP2761159B2 (ja) 1998-06-04
DE69215169D1 (de) 1996-12-19
US5795718A (en) 1998-08-18
CA2075858C (en) 1998-05-19
EP0530998B1 (en) 1996-11-13
EP0530998A1 (en) 1993-03-10
JPH06178700A (ja) 1994-06-28
CA2075858A1 (en) 1993-02-16
ATE145250T1 (de) 1996-11-15
DE69215169T2 (de) 1997-04-17
AU2105592A (en) 1993-02-18
AU653244B2 (en) 1994-09-22

Similar Documents

Publication Publication Date Title
ES2096041T3 (es) Deteccion de secuencias nucleotidicas complementarias.
JP7050848B2 (ja) リコンビナーゼポリメラーゼ増幅を多重化するための方法
EP0875583B1 (en) Primers and kits for the detection of Chlamydia trachomatis
CA1339872C (en) Homogeneous protection assay
EP0745690B1 (en) Detectably labeled dual conformation oligonucleotide Probes, Assays and Kits
CA2386115A1 (en) Dna sequencing method
AU2709995A (en) Method of amplification for increasing the sensitivity of detecting nucleic acid-probe target hybrids
FR2663040B1 (fr) Procede de detection d'une sequence nucleotidique selon la technique d'hybridation sandwich.
ATE427999T1 (de) Erkennung von nukleinsaurepolymorphismen
EP0892856B1 (en) A method for the amplification and detection of a nucleic acid fragment of interest
US5538872A (en) Method of preparing nucleotide probes using a bridging complement
KR20100075524A (ko) 표적 ｒｎａ의 검출·정량방법 및 키트
AUPP844899A0 (en) A method for detecting methylated nucleic acids
CA2165345A1 (en) Oligonucleotide screening assay
JP4209977B2 (ja) 淋菌の核酸の増幅および検出による淋菌の検出
KR970065730A (ko) 후 증폭 인큐베이팅 (post amplification incubation) 단계를 이용한 타겟 핵산의 증폭 및 검출 방법
EP0622464A2 (en) Nucleic acid assay procedure
ES2082881T3 (es) Procedimiento para la deteccion y determinacion cuantitativa de cepas de nitrosomonas en aguas residuales o suelos.
WO1990011374A1 (en) Polymerase chain reaction products incorporating reporter moieties and affinity seperation
AU7429591A (en) Nucleic acid probes for the detection of giardia lamblia
Dill et al. Detection of plasmids using DNA and RNA probes and the light-addressable potentiometric sensor
CA2172142A1 (en) Primers and probes for the amplification and detection of cmv nucleic acid
CA2482795A1 (en) Oligonucleotide probes directed at a target sequence in ck19 for detecting tumor cells
EP4276196A1 (en) Decoy-oligonucleotides in nucleic acid detection methods
KR100809679B1 (ko) 혼성화된 핵산의 검출 및 신호 증폭 방법

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 530998

Country of ref document: ES