ES2100286T3 - Identificacion de constituyentes de muestra utilizando electroforesis capilar. - Google Patents

Identificacion de constituyentes de muestra utilizando electroforesis capilar.

Info

Publication number
ES2100286T3
ES2100286T3 ES92303986T ES92303986T ES2100286T3 ES 2100286 T3 ES2100286 T3 ES 2100286T3 ES 92303986 T ES92303986 T ES 92303986T ES 92303986 T ES92303986 T ES 92303986T ES 2100286 T3 ES2100286 T3 ES 2100286T3
Authority
ES
Spain
Prior art keywords
species
identification
markers
capillary electrophoresis
constituent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92303986T
Other languages
English (en)
Inventor
Fu-Tai Albert Chen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beckman Coulter Inc
Original Assignee
Beckman Instruments Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beckman Instruments Inc filed Critical Beckman Instruments Inc
Application granted granted Critical
Publication of ES2100286T3 publication Critical patent/ES2100286T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
    • B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Electrochemistry (AREA)
  • Environmental & Geological Engineering (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Coloring (AREA)
  • Wire Bonding (AREA)
  • Glass Compositions (AREA)
  • Peptides Or Proteins (AREA)

Abstract

SE PRESENTAN MARCADORES EXTERNOS Y UN METODO PARA LA IDENTIFICACION DE MUESTRAS QUE CONTIENEN PROTEINAS Y PEPTIDOS UTILIZANDO UN ANALISIS DE ELECTROFORESIS CAPILAR. SEGUN SE DESCRIBE, AL MENOS DOS MARCADORES EXTERNOS SE AÑADEN A LA MUESTRA: UNO DE LOS MARCADORES ES UNA ESPECIE DE CARGA NEUTRAL Y EL OTRO MARCADOR ES UNA ESPECIE IONICA. EL DIMETILFORMAMIDO ES LA ESPECIE DE CARGA NEUTRA MAS ADECUADA Y EL ACIDO BENZOICO ES LA ESPECIE IONICA MAS ADECUADA. LA IDENTIFICACION DE LAS ESPECIES CONSTITUYENTES SE DETERMINA COMPARANDO LAS PROPORCIONES DE MIGRACION DE LAS ESPECIES CONSTITUYENTES CON LAS RELACIONES DE MIGRACION PARA CADA MIEMBRO DE LAS ESPECIES CONSTITUYENTES.
ES92303986T 1991-05-31 1992-05-01 Identificacion de constituyentes de muestra utilizando electroforesis capilar. Expired - Lifetime ES2100286T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/708,424 US5139630A (en) 1991-05-31 1991-05-31 Identification of sample constituents utilizing capillary electrophoresis

Publications (1)

Publication Number Publication Date
ES2100286T3 true ES2100286T3 (es) 1997-06-16

Family

ID=24845754

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92303986T Expired - Lifetime ES2100286T3 (es) 1991-05-31 1992-05-01 Identificacion de constituyentes de muestra utilizando electroforesis capilar.

Country Status (8)

Country Link
US (1) US5139630A (es)
EP (1) EP0518476B1 (es)
JP (1) JP3085786B2 (es)
AT (1) ATE151534T1 (es)
AU (1) AU654709B2 (es)
CA (1) CA2069031C (es)
DE (1) DE69218833T2 (es)
ES (1) ES2100286T3 (es)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4237105A1 (de) * 1992-11-03 1994-05-05 Behringwerke Ag Verfahren zur eindimensionalen Auftrennung und Identifizierung von Glycanen, sowie die Verwendung dieses Verfahrens zur Erstellung von Datenbanken und zur Strukturermittlung von Glycanen
AU6114194A (en) * 1993-03-09 1994-09-26 Inopsia Bron & Co. Method for automatically correcting and matching digitized electrophoresis and western blots tests
AU669308B2 (en) * 1993-10-07 1996-05-30 Beckman Instruments, Inc. Use of capillary electrophoresis for quantitating the concentration of protein components and of the total proteinin fluids
US5567282A (en) * 1994-01-25 1996-10-22 Wang; Hann-Ping On-capillary electrophoretic immunosubtraction for classification and typing of M-proteins
EP0704053A1 (en) * 1994-04-13 1996-04-03 Beckman Instruments, Inc. Analysis of analytes in biological fluids
US5599433A (en) * 1995-01-17 1997-02-04 Beckman Instruments, Inc. Capillary electrophoresis of glycosylated proteins
US5582705A (en) * 1995-05-19 1996-12-10 Iowa State University Research Foundation, Inc. Multiplexed capillary electrophoresis system
US5753094A (en) * 1995-09-20 1998-05-19 Beckman Instruments, Inc. Borate storage buffer and sample diluent
EP0963435B1 (en) 1997-01-08 2008-05-28 Invitrogen Corporation Methods for production of proteins
US5932080A (en) * 1997-05-30 1999-08-03 Bio-Rad Laboratories, Inc. Mobility-based and normalized capillary electrophoresis
EP1044370B1 (en) * 1997-12-30 2017-08-23 Caliper Life Sciences, Inc. Software for the display of chromatographic separation data
US7713703B1 (en) 2000-11-13 2010-05-11 Biosite, Inc. Methods for monitoring the status of assays and immunoassays
US6194222B1 (en) * 1998-01-05 2001-02-27 Biosite Diagnostics, Inc. Methods for monitoring the status of assays and immunoassays
US6821402B1 (en) * 1998-09-16 2004-11-23 Applera Corporation Spectral calibration of fluorescent polynucleotide separation apparatus
US6168701B1 (en) * 1999-04-30 2001-01-02 The Perkins-Elmer Corporation Methods and compositions for improving the loading of analytical instruments
US6974527B2 (en) * 2000-06-06 2005-12-13 Spectrumedix Llc Multidimensional separations employing an array of electrophoresis channels
US20040014082A1 (en) * 2000-08-11 2004-01-22 Invitrogen Corporation Highly homogeneous molecular markers for electrophoresis
JP2004506221A (ja) * 2000-08-11 2004-02-26 インヴィトロジェン　コーポレーション 高度に均一な電気泳動用分子マーカー
CA2477159A1 (en) * 2002-02-20 2003-08-28 Invitrogen Corporation Highly homogeneous molecular markers for electrophoresis
US7381317B2 (en) * 2002-08-12 2008-06-03 Beckman Coulter, Inc. Methods and compositions for capillary electrophoresis (CE)
EP1668146B1 (en) 2003-09-25 2014-11-12 Life Technologies Corporation Homogeneous populations of molecules
US20050115837A1 (en) * 2003-12-01 2005-06-02 Dean Burgi Analyte identification in transformed electropherograms
US20070014699A1 (en) 2005-06-23 2007-01-18 Beckman Coulter, Inc, Methods and apparatus for improving the sensitivity of capillary zone electrophoresis
JP4742712B2 (ja) * 2005-07-14 2011-08-10 株式会社島津製作所 キャピラリー電気泳動方法
CA2646944A1 (en) * 2007-11-30 2009-05-30 Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of National Defence Serum components that bind to threat agents

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4994165A (en) * 1989-02-16 1991-02-19 Cornell Research Foundation, Inc. Liquid junction coupling for capillary zone electrophoresis/ion spray spectrometry
US4941958A (en) * 1989-03-08 1990-07-17 Westinghouse Electric Corp. Device and method for detecting ionic components in solution
US5021646A (en) * 1990-01-25 1991-06-04 Spectra-Physics, Inc. Remote optical path for capillary electrophoresis instrument

Also Published As

Publication number Publication date
JPH05196604A (ja) 1993-08-06
US5139630A (en) 1992-08-18
CA2069031C (en) 1996-08-27
AU1628892A (en) 1992-12-03
JP3085786B2 (ja) 2000-09-11
EP0518476A1 (en) 1992-12-16
CA2069031A1 (en) 1992-12-01
ATE151534T1 (de) 1997-04-15
DE69218833T2 (de) 1997-07-17
EP0518476B1 (en) 1997-04-09
DE69218833D1 (de) 1997-05-15
AU654709B2 (en) 1994-11-17

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