ES2100357T3 - Aparato para la manipulacion de membranas de transferencia electroforetica y para la deteccion quimioluminiscente de manchas contenidas en las mismas, asi como procedimientos en los que se emplea dicho aparato. - Google Patents
Aparato para la manipulacion de membranas de transferencia electroforetica y para la deteccion quimioluminiscente de manchas contenidas en las mismas, asi como procedimientos en los que se emplea dicho aparato.Info
- Publication number
- ES2100357T3 ES2100357T3 ES92916459T ES92916459T ES2100357T3 ES 2100357 T3 ES2100357 T3 ES 2100357T3 ES 92916459 T ES92916459 T ES 92916459T ES 92916459 T ES92916459 T ES 92916459T ES 2100357 T3 ES2100357 T3 ES 2100357T3
- Authority
- ES
- Spain
- Prior art keywords
- membrane
- handling
- detection
- disclosed
- quimioluminiscent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000012528 membrane Substances 0.000 title abstract 7
- 238000012546 transfer Methods 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 238000001514 detection method Methods 0.000 title abstract 2
- BVTJGGGYKAMDBN-UHFFFAOYSA-N Dioxetane Chemical class C1COO1 BVTJGGGYKAMDBN-UHFFFAOYSA-N 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 238000001035 drying Methods 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 abstract 1
- 230000001960 triggered effect Effects 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44739—Collecting the separated zones, e.g. blotting to a membrane or punching of gel spots
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Electrochemistry (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
SE PRESENTA UN APARATO MEJORADO PARA EL MANEJO Y DETECCION DE UNA MEMBRANA DE TRANSFERENCIA ELECTROFORETICA, LA MEJORA COMPRENDE UN APARATO DE GUIA Y SEÑAL PARA ACOMODAR LA MEMBRANA DE TRANSFERENCIA, UNA CABEZA DE PRESION POSICIONADA CON RELACION AL APARATO DE GUIA PARA ENGANCHAR DE FORMA LIBERABLE LA MEMBRANA, Y UN APARATO DE PROTECCION DE LA LUZ POSICIONADA CON RELACION AL APARATO DE GUIA PARA ENCERRAR SELECTIVAMENTE LA MEMBRANA DE TRANSFERENCIA. EL APARATO ES ACCIONADO DE MANERA QUE LA CABEZA DE PRESION ENGANCHE UN APARTE FLEXIBLE DE LA MEMBRANA DE TRANSFERENCIA QUE CONTIENE UNA MANCHA ELECTROFORETICA MIENTRAS QUE ESTA EN UNA POSICION APROPIADA PARA LA DETECCION. TAMBIEN SE PRESENTA UN PROCESO QUE UTILIZA EL APARATO DE LA INVENCION. EN EL PROCESO, SE PRESENTA EL SECADO Y EL CALENTAMIENTO OPCIONAL DE LA MEMBRANA EN DONDE SE HA CONTACTO LA MANCHA CON 1,2DIOXETANOS INICIADOS CON UNA ENZIMA, PARA MEJORAR LA SEÑAL QUIMIOLUMINISCENTE RESULTANTE.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US73912391A | 1991-07-31 | 1991-07-31 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2100357T3 true ES2100357T3 (es) | 1997-06-16 |
Family
ID=24970926
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES92916459T Expired - Lifetime ES2100357T3 (es) | 1991-07-31 | 1992-07-28 | Aparato para la manipulacion de membranas de transferencia electroforetica y para la deteccion quimioluminiscente de manchas contenidas en las mismas, asi como procedimientos en los que se emplea dicho aparato. |
Country Status (16)
| Country | Link |
|---|---|
| EP (1) | EP0596980B1 (es) |
| JP (1) | JP3112941B2 (es) |
| AT (1) | ATE152521T1 (es) |
| AU (1) | AU2398692A (es) |
| CA (1) | CA2113620A1 (es) |
| DE (1) | DE69219474T2 (es) |
| DK (1) | DK0596980T3 (es) |
| ES (1) | ES2100357T3 (es) |
| GR (1) | GR3023988T3 (es) |
| HK (1) | HK115597A (es) |
| IE (1) | IE922463A1 (es) |
| IL (1) | IL102524A0 (es) |
| LT (1) | LTIP684A (es) |
| LV (1) | LV10131B (es) |
| MX (1) | MX9204449A (es) |
| WO (1) | WO1993003359A1 (es) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5474663A (en) * | 1993-08-27 | 1995-12-12 | E. I. Du Pont De Nemours And Company | Bowed framed membrane, processes for the preparation thereof, and uses therefor |
| JP2001083162A (ja) * | 1999-09-17 | 2001-03-30 | Fuji Photo Film Co Ltd | 膜状生体高分子吸着材及び吸着用キット |
| US20150219597A1 (en) * | 2012-05-31 | 2015-08-06 | Ge Healthcare Bio-Sciences Ab | Method of manufacturing an electrophoresis cassette |
| IN2013DE03479A (es) | 2013-11-29 | 2015-06-05 | Ge Healthcare Bio Sciences Ab | |
| WO2015078781A1 (en) * | 2013-11-29 | 2015-06-04 | Ge Healthcare Bio-Sciences Ab | Integrated system for electro-blotting |
| JP5952446B1 (ja) | 2015-02-24 | 2016-07-13 | シャープ株式会社 | 転写膜保持器具及び分離転写装置 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4152390A (en) * | 1976-12-17 | 1979-05-01 | Eastman Kodak Company | Chemical analyzer |
| US4389670A (en) * | 1981-12-30 | 1983-06-21 | The United States Of America As Represented By The United States Department Of Energy | Electronic method for autofluorography of macromolecules on two-D matrices |
| GB8513538D0 (en) * | 1985-05-29 | 1985-07-03 | Mackay C D | Electrophoresis |
| US4931223A (en) * | 1986-07-24 | 1990-06-05 | Tropix, Inc. | Methods of using chemiluminescent 1,2-dioxetanes |
| US4913791A (en) * | 1987-08-28 | 1990-04-03 | Bios Corporation | Blot frame and method of handling |
| US4952707A (en) * | 1988-06-30 | 1990-08-28 | Tropix, Inc. | Enzymatically-cleavable chemiluminescent fused polycyclic ring-containing 1,2-dioxetanes |
-
1992
- 1992-07-16 IL IL102524A patent/IL102524A0/xx unknown
- 1992-07-28 DK DK92916459.8T patent/DK0596980T3/da active
- 1992-07-28 CA CA002113620A patent/CA2113620A1/en not_active Abandoned
- 1992-07-28 EP EP92916459A patent/EP0596980B1/en not_active Expired - Lifetime
- 1992-07-28 AU AU23986/92A patent/AU2398692A/en not_active Abandoned
- 1992-07-28 DE DE69219474T patent/DE69219474T2/de not_active Expired - Fee Related
- 1992-07-28 AT AT92916459T patent/ATE152521T1/de not_active IP Right Cessation
- 1992-07-28 WO PCT/US1992/006114 patent/WO1993003359A1/en not_active Ceased
- 1992-07-28 ES ES92916459T patent/ES2100357T3/es not_active Expired - Lifetime
- 1992-07-28 JP JP05503085A patent/JP3112941B2/ja not_active Expired - Fee Related
- 1992-07-29 IE IE246392A patent/IE922463A1/en not_active Application Discontinuation
- 1992-07-30 MX MX9204449A patent/MX9204449A/es unknown
-
1993
- 1993-05-12 LV LVP-93-324A patent/LV10131B/en unknown
- 1993-06-23 LT LTIP684A patent/LTIP684A/xx not_active Application Discontinuation
-
1997
- 1997-06-26 HK HK115597A patent/HK115597A/en not_active IP Right Cessation
- 1997-07-02 GR GR970401642T patent/GR3023988T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| JPH06509867A (ja) | 1994-11-02 |
| IE922463A1 (en) | 1993-02-10 |
| DE69219474T2 (de) | 1997-12-18 |
| EP0596980B1 (en) | 1997-05-02 |
| IL102524A0 (en) | 1993-01-14 |
| JP3112941B2 (ja) | 2000-11-27 |
| LV10131A (lv) | 1994-05-10 |
| ATE152521T1 (de) | 1997-05-15 |
| GR3023988T3 (en) | 1997-10-31 |
| LTIP684A (en) | 1995-01-31 |
| LV10131B (en) | 1995-04-20 |
| MX9204449A (es) | 1993-04-01 |
| EP0596980A1 (en) | 1994-05-18 |
| AU2398692A (en) | 1993-03-02 |
| CA2113620A1 (en) | 1993-02-18 |
| DE69219474D1 (de) | 1997-06-05 |
| HK115597A (en) | 1997-08-29 |
| DK0596980T3 (da) | 1997-11-03 |
| WO1993003359A1 (en) | 1993-02-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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