ES2104568T3 - Adn recombinante y vector de expresion. - Google Patents

Adn recombinante y vector de expresion.

Info

Publication number
ES2104568T3
ES2104568T3 ES90107465T ES90107465T ES2104568T3 ES 2104568 T3 ES2104568 T3 ES 2104568T3 ES 90107465 T ES90107465 T ES 90107465T ES 90107465 T ES90107465 T ES 90107465T ES 2104568 T3 ES2104568 T3 ES 2104568T3
Authority
ES
Spain
Prior art keywords
recombinant dna
gene
expression vector
sequence
brought
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90107465T
Other languages
English (en)
Inventor
August Prof Dr Bock
Robert Gary Dr Rer Nat Sawers
Michael Dr Rer Nat Jarsch
Roland Herbst
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Application granted granted Critical
Publication of ES2104568T3 publication Critical patent/ES2104568T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

EL DNA RECOMBINANTE SEGUN EL INVENTO CONTIENE UNA REGION REGULADORA HOMOLOGA EN UN 50 %, COMO MINIMO, CON LAS ZONAS PARCIALES DE LOS PARES DE BASES 969 A 991 Y/O DE LOS 1308 A 1330 DE LA SECUENCIA DE LA FIGURA 10 Y UNA REGION PROMOTORA, COMO MINIMO, EN DIRECCION 3'' DE LA SECUENCIA DE REGULACION, LA CUAL CONTIENE A SU VEZ UNA SECUENCIA PROMOTORA 35/10. UN VECTOR DE EXPRESION CONFORME AL INVENTO CONTIENE UN DNA RECOMBINANTE, CONFORME AL INVENTO, LIGADO A UNA MOLECULA VECTOR APROPIADA. PARA LA PREPARACION DEL DNA RECOMBINANTE, CONFORME AL INVENTO, SE AISLA UN MICROORGANISMO DEL BANCO DE GENES, QUE CONTENGA UN GEN PFL, CON SUS REGIONES UPSTREAM. SIGUIENDO METODOS EN SI YA CONOCIDOS, SE SEPARAN LAS PARTES QUE SE DESEAN Y SE LAS UNE CON LAS OTRAS SECUENCIAS O PROMOTORES DESEADOS. SI SE UTILIZA UN DNA RECOMBINANTE O UN VECTOR DE EXPRESION, CONFORMES AL INVENTO, PARA LA EXPRESION INDUCIBLE O REPRIMIBLE DE UN GEN EXTRAÑO, LA INCUBACION SE REALIZA BAJO CONDICIONES ANAEROBIAS Y A TRAVES DE PIRUVATO, Y LA REPRESION A TRAVES DE OXIGENO.
ES90107465T 1989-04-21 1990-04-19 Adn recombinante y vector de expresion. Expired - Lifetime ES2104568T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3913201 1989-04-21
DE3926076A DE3926076A1 (de) 1989-04-21 1989-08-07 Rekombinante dna und expressionsvektor

Publications (1)

Publication Number Publication Date
ES2104568T3 true ES2104568T3 (es) 1997-10-16

Family

ID=25880155

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90107465T Expired - Lifetime ES2104568T3 (es) 1989-04-21 1990-04-19 Adn recombinante y vector de expresion.

Country Status (7)

Country Link
EP (1) EP0393684B1 (es)
JP (1) JP2774989B2 (es)
AT (1) ATE154639T1 (es)
CA (1) CA2015046C (es)
DE (2) DE3926076A1 (es)
DK (1) DK0393684T3 (es)
ES (1) ES2104568T3 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9104596D0 (en) * 1991-03-05 1991-04-17 Wellcome Found Production of recombinant proteins
JP3415145B2 (ja) * 1991-03-05 2003-06-09 ザ ウエルカム ファウンデーション リミテッド 弱毒化細菌における組換えタンパク質の発現
DE4136389A1 (de) * 1991-03-18 1992-10-08 Hoechst Ag Verfahren zur herstellung von glutarylacylase in grossen mengen
US5830692A (en) * 1995-03-24 1998-11-03 Consortium Fur Elektrochemische Industrie Gmbh Expression system which can be regulated
KR101643195B1 (ko) * 2014-10-06 2016-07-27 경일대학교산학협력단 수중 객체를 탐지하기 위한 헬멧 장치 및 이를 위한 방법

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3735381A1 (de) * 1987-03-31 1989-05-03 Boehringer Mannheim Gmbh Rekombinante dna zur reprimierbaren und induzierbaren expression von fremdgenen

Also Published As

Publication number Publication date
JPH0380088A (ja) 1991-04-04
DK0393684T3 (da) 1998-01-26
EP0393684A1 (de) 1990-10-24
CA2015046C (en) 2003-09-16
ATE154639T1 (de) 1997-07-15
JP2774989B2 (ja) 1998-07-09
CA2015046A1 (en) 1990-10-21
DE3926076A1 (de) 1990-10-25
DE59010726D1 (de) 1997-07-24
EP0393684B1 (de) 1997-06-18

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