ES2108733T3 - Metodo y medios para centrifugacion por gradiente de densidad. - Google Patents
Metodo y medios para centrifugacion por gradiente de densidad.Info
- Publication number
- ES2108733T3 ES2108733T3 ES92311300T ES92311300T ES2108733T3 ES 2108733 T3 ES2108733 T3 ES 2108733T3 ES 92311300 T ES92311300 T ES 92311300T ES 92311300 T ES92311300 T ES 92311300T ES 2108733 T3 ES2108733 T3 ES 2108733T3
- Authority
- ES
- Spain
- Prior art keywords
- centrifugation
- insert
- gradient density
- layering
- vessel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000005119 centrifugation Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 title 1
- 238000000432 density-gradient centrifugation Methods 0.000 abstract 1
- 239000006185 dispersion Substances 0.000 abstract 1
- 230000005484 gravity Effects 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
- G01N33/491—Blood by separating the blood components
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Urology & Nephrology (AREA)
- Ecology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Centrifugal Separators (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- External Artificial Organs (AREA)
Abstract
EN LA REALIZACION DE CENTRIFUGACION CON GRADIENTE DE DENSIDAD EN MEZCLAS DESPUES DE LA CENTRIFUGACION SE HA PREVISTO LA COLOCACION DE UN SALIENTE AJUSTADO MEDIANTE INSERCION EN CAPAS EN EL INTERIOR DEL RECIPIENTE. LA PARTE INSERTADA ES CAPAZ DE SOPORTAR EL CUERPO DEL LIQUIDO EN DISPERSION PARA SER CENTRIFUGADO BAJO LAS CONDICIONES DE GRAVEDAD REGULAR Y PERMITE EL CRUCE DE FLUJO BIDIRECCIONAL DURANTE LA CENTRIFUGACION. UNA PLACA PEQUEÑA CON MASA DE FILTRO PUEDE SER COLOCADA EN LA PARTE SUPERIOR DE LA PIEZA INSERTADA EN FORMA DE CAPAS.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IL10082892A IL100828A (en) | 1992-01-31 | 1992-01-31 | Method and means for density gradient centrifugation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2108733T3 true ES2108733T3 (es) | 1998-01-01 |
Family
ID=11063334
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES92311300T Expired - Lifetime ES2108733T3 (es) | 1992-01-31 | 1992-12-10 | Metodo y medios para centrifugacion por gradiente de densidad. |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US5314074A (es) |
| EP (1) | EP0553554B1 (es) |
| JP (1) | JP3325620B2 (es) |
| AT (1) | ATE155582T1 (es) |
| DE (1) | DE69220917T2 (es) |
| ES (1) | ES2108733T3 (es) |
| IL (1) | IL100828A (es) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5431814A (en) * | 1993-10-22 | 1995-07-11 | Jorgensen; Glen | Centrifugal filter apparatus and method |
| US5648223A (en) * | 1994-08-31 | 1997-07-15 | Activated Cell Therapy, Inc. | Methods for enriching breast tumor cells |
| US5646004A (en) * | 1994-08-31 | 1997-07-08 | Activated Cell Therapy, Inc. | Methods for enriching fetal cells from maternal body fluids |
| DE69513188T2 (de) * | 1994-08-31 | 2000-07-06 | Dendreon Corp., Seattle | Vorrichtung und verfahren zur trennung von zellen |
| US5707331A (en) * | 1995-05-05 | 1998-01-13 | John R. Wells | Automatic multiple-decanting centrifuge |
| AUPO488697A0 (en) * | 1997-01-31 | 1997-02-20 | Australian Red Cross Society (Western Australian Division) | Method and means for separating blood |
| US6390966B2 (en) * | 2000-04-18 | 2002-05-21 | Large Scale Proteomics Corporation | Method for making density gradients |
| PT1289618E (pt) | 2000-04-28 | 2008-04-11 | Harvest Technologies Corp | Disco separador de componentes do sangue |
| DK2129595T3 (da) | 2007-03-23 | 2011-04-18 | Nestec Sa | Drikkevareingredienskapsel |
| US8177072B2 (en) | 2008-12-04 | 2012-05-15 | Thermogenesis Corp. | Apparatus and method for separating and isolating components of a biological fluid |
| KR101197974B1 (ko) * | 2011-11-01 | 2012-11-05 | 박현정 | 신속한 원심분리가 가능한 원심분리용 용기 |
| GB201418382D0 (en) | 2014-10-16 | 2014-12-03 | Ge Healthcare Uk Ltd | Device and method for separating cells |
| CN104593221B (zh) * | 2015-01-13 | 2016-10-19 | 中国科学院生物物理研究所 | 可调多层细胞分离筛装置 |
| PL237582B1 (pl) * | 2015-09-15 | 2021-05-04 | Spark Tech Spolka Z Ograniczona Odpowiedzialnoscia | Insert do pojemnika do wirowania, zwłaszcza, do probówki, do wydzielenia frakcji o pożądanym zakresie gęstości metodą wirowania na gradiencie gęstości oraz pojemnik do wirowania zawierający ten insert |
| WO2017046736A1 (en) * | 2015-09-15 | 2017-03-23 | Spark-Tech Sp. Z O.O. | Device and method for fluids separation by density gradient |
| JP7733073B2 (ja) * | 2023-07-18 | 2025-09-02 | H.U.グループホールディングス株式会社 | 分離インサート、及び分離器具 |
| CN118086051A (zh) * | 2024-04-10 | 2024-05-28 | 福建省妇幼保健院 | 一种可充分灵活回收活动精子的精子上游管 |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4001122A (en) * | 1973-08-22 | 1977-01-04 | Telan Corporation | Method and device for separating blood components |
| US3931010A (en) * | 1974-02-27 | 1976-01-06 | Becton, Dickinson And Company | Serum/plasma separators with centrifugal valves |
| US4083788A (en) * | 1975-11-19 | 1978-04-11 | Ferrara Louis T | Blood serum-isolation device |
| US4257886A (en) * | 1979-01-18 | 1981-03-24 | Becton, Dickinson And Company | Apparatus for the separation of blood components |
| US4224942A (en) * | 1979-04-25 | 1980-09-30 | Northwestern University | Cell fractionating method |
| US4417981A (en) * | 1981-05-04 | 1983-11-29 | Becton, Dickinson And Company | Blood phase separator device |
| US4492634A (en) * | 1982-09-28 | 1985-01-08 | Emde Medical Research | Pre-evacuated blood collection tube with anti-hemolysis baffle system and centrifugation propelled filtration disc and efficient serum-from cells separator |
| US5053134A (en) * | 1984-12-04 | 1991-10-01 | Becton Dickinson And Company | Lymphocyte collection tube |
| CA1291098C (en) * | 1984-12-04 | 1991-10-22 | Albert August Luderer | Lymphocyte collection tube |
| US4722792A (en) * | 1985-02-09 | 1988-02-02 | Kurashiki Boseki Kabushiki Kaisha | Filter for centrifugal separator |
| IL74967A (en) * | 1985-04-18 | 1988-10-31 | Assaf Pharmaceutical Ind | Separation of materials from a liquid dispersion by sedimentation |
| US4602995A (en) * | 1985-05-20 | 1986-07-29 | Technicon Instruments Corporation | Liquid level adjusting and filtering device |
| US4636361A (en) * | 1985-11-18 | 1987-01-13 | Miklos Marian | Device for separating liquid fractions |
| US4683058A (en) * | 1986-03-20 | 1987-07-28 | Costar Corporation | Filter for centrifuge tube |
| US4822495A (en) * | 1987-07-07 | 1989-04-18 | St. Mary's Hospital And Medical Center | Cell block collection method and apparatus |
| US4818386A (en) * | 1987-10-08 | 1989-04-04 | Becton, Dickinson And Company | Device for separating the components of a liquid sample having higher and lower specific gravities |
| US4844818A (en) * | 1987-10-23 | 1989-07-04 | Becton Dickinson & Company | Method for separating the cellular components of blood samples |
-
1992
- 1992-01-31 IL IL10082892A patent/IL100828A/en not_active IP Right Cessation
- 1992-11-03 US US07/971,525 patent/US5314074A/en not_active Expired - Fee Related
- 1992-12-10 EP EP92311300A patent/EP0553554B1/en not_active Expired - Lifetime
- 1992-12-10 ES ES92311300T patent/ES2108733T3/es not_active Expired - Lifetime
- 1992-12-10 AT AT92311300T patent/ATE155582T1/de not_active IP Right Cessation
- 1992-12-10 DE DE69220917T patent/DE69220917T2/de not_active Expired - Fee Related
- 1992-12-16 JP JP33623992A patent/JP3325620B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US5314074A (en) | 1994-05-24 |
| IL100828A0 (en) | 1992-09-06 |
| EP0553554B1 (en) | 1997-07-16 |
| DE69220917D1 (de) | 1997-08-21 |
| JPH06206008A (ja) | 1994-07-26 |
| EP0553554A1 (en) | 1993-08-04 |
| IL100828A (en) | 2002-05-23 |
| ATE155582T1 (de) | 1997-08-15 |
| JP3325620B2 (ja) | 2002-09-17 |
| DE69220917T2 (de) | 1998-09-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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