ES2108850T3 - Nitrilasa recombinante y su utilizacion. - Google Patents

Nitrilasa recombinante y su utilizacion.

Info

Publication number
ES2108850T3
ES2108850T3 ES93420334T ES93420334T ES2108850T3 ES 2108850 T3 ES2108850 T3 ES 2108850T3 ES 93420334 T ES93420334 T ES 93420334T ES 93420334 T ES93420334 T ES 93420334T ES 2108850 T3 ES2108850 T3 ES 2108850T3
Authority
ES
Spain
Prior art keywords
polypeptide
nitrile
nitrilase activity
genetic
recombinant nitrilase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93420334T
Other languages
English (en)
Inventor
Dominique Petre
Edith Cerbelaud
Sophie Levy-Schil
Joel Crouzet
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Adisseo France SAS
Original Assignee
Rhone Poulenc Nutrition Animale SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rhone Poulenc Nutrition Animale SA filed Critical Rhone Poulenc Nutrition Animale SA
Application granted granted Critical
Publication of ES2108850T3 publication Critical patent/ES2108850T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/05—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in nitriles (3.5.5)
    • C12Y305/05001—Nitrilase (3.5.5.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

LA PRESENTE INVENCION TIENE POR OBJETO A POLIPEPTIDOS QUE PRESENTAN UNA ACTIVIDAD NITRILASA, ASI COMO LOS INSTRUMENTOS GENETICOS PARA PRODUCIRLOS, A SABER: FICANTE PARA UN POLIPEPTIDO QUE TIENE UNA ACTIVIDAD NITRILASA Y CAPAZ DE HIDROLIZAR LOS NITRILOS EN CARBOXILATOS, GO DE ESTA SECUENCIA QUE RESULTA DE LA DEGENERACION DEL CODIGO GENETICO, UENCIAS O UN FRAGMENTO DE ESTAS Y DOSIFICANTE PARA UN POLIPEPTIDO QUE TIENE UNA ACTIVIDAD NITRILASA, CASSETTES DE EXPRESION Y MICROORGANISMOS QUE PERMITEN SU OBTENCION. APLICACION: TRANSFORMACION ENZIMATICA DE LOS NITRILOS EN CARBOXILATOS.
ES93420334T 1992-08-10 1993-08-06 Nitrilasa recombinante y su utilizacion. Expired - Lifetime ES2108850T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9209882A FR2694571B1 (fr) 1992-08-10 1992-08-10 Polypeptides possédant une activité nitrilase, séquence d'ADN codant pour lesdits polypeptides, cassettes d'expression et micro-organismes hôtes permettant leur obtention.

Publications (1)

Publication Number Publication Date
ES2108850T3 true ES2108850T3 (es) 1998-01-01

Family

ID=9432757

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93420334T Expired - Lifetime ES2108850T3 (es) 1992-08-10 1993-08-06 Nitrilasa recombinante y su utilizacion.

Country Status (10)

Country Link
EP (1) EP0596812B1 (es)
JP (1) JPH0751070A (es)
KR (1) KR940004057A (es)
BR (1) BR9305280A (es)
CA (1) CA2103616A1 (es)
DE (1) DE69314280T2 (es)
ES (1) ES2108850T3 (es)
FR (1) FR2694571B1 (es)
MX (1) MX9304825A (es)
SG (1) SG48047A1 (es)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU669951B2 (en) * 1994-01-12 1996-06-27 Rhone-Poulenc Nutrition Animale Polypeptides possessing a nitrilase activity, DNA sequence coding for said polypeptides, expression cassettes and host microorganisms enabling them to be obtained, and method of converting nitriles to carboxylates by means of said polypeptides
US5858736A (en) * 1996-05-17 1999-01-12 E. I. Du Pont De Nemours And Company Preparation of lactams from aliphatic α,ω-dinitriles
FR2780416B1 (fr) * 1998-06-10 2002-12-20 Rhone Poulenc Nutrition Animal Procede industriel de production de proteines heterologues chez e. coli et souches utiles pour le procede
US6365376B1 (en) 1999-02-19 2002-04-02 E. I. Du Pont De Nemours And Company Genes and enzymes for the production of adipic acid intermediates
US6551804B2 (en) 1999-07-12 2003-04-22 E. I. Du Pont De Nemours And Company Process for preparing 4-cyanopentanoic acid
US7521216B2 (en) 1999-12-29 2009-04-21 Verenium Corporation Nitrilases and methods for making and using them
US7608445B1 (en) 1999-12-29 2009-10-27 Verenium Corporation Nitrilases, nucleic acids encoding them and methods for making and using them
US7300775B2 (en) 1999-12-29 2007-11-27 Verenium Corporation Methods for producing α-substituted carboxylic acids using nitrilases and strecker reagents
AU4972601A (en) 2000-03-31 2001-10-15 Du Pont Isolation and expression of a gene for a nitrilase from acidovorax facilis 72w
US6562603B2 (en) 2000-08-04 2003-05-13 E. I. Du Pont De Nemours And Company 3-hydroxycarboxylic acid production and use in branched polymers
AU2002213028A1 (en) 2000-09-30 2002-04-15 North Carolina State University Exbb-exbd-tonb gene cluster of pseudomonas putida dot-t1e conferring tolerance to aromatic compounds and resistance to antibiotics
FR2822460B1 (fr) * 2001-03-26 2005-04-29 Rhodia Chimie Sa Procede de preparation enantioselectif d'acides carboxyliques optiquement actifs par hydrolyse enzymatique de nitriles
US6670158B2 (en) * 2002-02-05 2003-12-30 E. I. Du Pont De Nemours And Company Method for producing methacrylic acid acrylic acid with a combination of enzyme catalysts
US7198926B2 (en) 2003-05-08 2007-04-03 E. I. Du Pont De Nemours And Company Preparation of (E)- and (Z)-2-methyl-2-butenoic acids
US7148051B2 (en) 2004-08-16 2006-12-12 E. I. Du Pont De Nemours And Company Production of 3-hydroxycarboxylic acid using nitrilase
DE102004043748A1 (de) * 2004-09-10 2006-03-16 Degussa Ag Biokatalysator zur Hydrolyse von Cyanid
KR101312262B1 (ko) * 2006-02-23 2013-09-25 삼성에스디아이 주식회사 고분자막, 그 제조방법 및 이를 채용한 연료전지
EP2115153B1 (en) 2007-03-01 2013-06-05 BP Corporation North America Inc. Nitrilases, nucleic acids encoding them and methods for making and using them
KR101223664B1 (ko) * 2011-01-26 2013-01-17 재단법인 경북해양바이오산업연구원 나이트릴레이즈 ｒｍｎ１을 이용한 카르복실산의 제조방법
GB2554708A (en) * 2016-10-05 2018-04-11 Chemoxy Int Process for converting nitriles
CN113614242A (zh) * 2019-01-22 2021-11-05 巴斯夫欧洲公司 4-氰基苯甲酸或其盐的生产方法
CN112359036B (zh) * 2020-11-30 2022-03-08 浙江工业大学 一种催化活力和反应专一性提高的腈水解酶突变体及应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4629700A (en) * 1983-11-30 1986-12-16 Standard Oil Company (Indiana) Selective conversion of cyano compounds to amides and carboxylic acids
US4810648A (en) * 1986-01-08 1989-03-07 Rhone Poulenc Agrochimie Haloarylnitrile degrading gene, its use, and cells containing the gene
DE3926103A1 (de) * 1989-08-08 1991-02-14 Hoechst Ag Verfahren zur biokatalytischen korrekten kettenfaltung denaturierter rekombinanter fusionsproteine

Also Published As

Publication number Publication date
KR940004057A (ko) 1994-03-14
EP0596812B1 (fr) 1997-10-01
EP0596812A1 (fr) 1994-05-11
JPH0751070A (ja) 1995-02-28
MX9304825A (es) 1994-05-31
FR2694571B1 (fr) 1994-11-04
BR9305280A (pt) 1994-06-28
FR2694571A1 (fr) 1994-02-11
DE69314280T2 (de) 1998-02-05
SG48047A1 (en) 1998-04-17
CA2103616A1 (en) 1994-02-11
DE69314280D1 (de) 1997-11-06

Similar Documents

Publication Publication Date Title
ES2108850T3 (es) Nitrilasa recombinante y su utilizacion.
BR9714513A (pt) Genes que codificam para aminoácido-deacetilases com especificidade para n-acetil-l-fosfinotricina, seu isolamento e emprego
ES2124532T3 (es) Sistema vector-huesped utilizable en terapia genica.
ES2163864T3 (es) Secuencias de dna de 2-desoxiglucosa-6-fosfato (2-dog-6-p) como marcador de seleccion en plantas.
ES2120036T3 (es) Virus recombinantes y su utilizacion en terapia genetica.
MX9801008A (es) Constructos de adn.
PT87562A (pt) Procede de expression de la proapolipoproteine a-i humaine
ES2074042T3 (es) Exotoxina recombinante de pseudomonas construccion de una inmunotoxina activa con efectos secundarios menores.
ES2164182T3 (es) Benzociclopentanoxazolidinonas que contienen heteroatomos con actividad antibacteriana.
HUP0003924A2 (hu) Génterápiás megközelítések ApoA-I agonisták biztosítására, valamint ezek alkalmazása diszlipidémiás rendellenességek kezelésében
ES2179077T3 (es) Acido glutamico decarboxilasa clonada.
NO1996012I1 (no) Betaferon
ES2054638T3 (es) Gen codificante de los factores humanos de estimulacion colonial.
ATE476495T1 (de) Bazillus thuringiensis und dessen insektizide proteine
ES2185695T3 (es) Terapia genica dirigida.
ES2061520T3 (es) Expresion de un antigeno especifico de tumor por un virus vector recombinante y utilizacion de este para el tratamiento preventivo o curativo del tumor correspondiente.
FI885504A0 (fi) Vaskulaera antikoagulerande proteiner, dessa koderande dna, deras framstaellningsfoerfarande och deras anvaendning.
ES2124470T3 (es) Lectina recombinante de muerdago.
PT94680A (pt) Processo para a obtencao de uma proteina tendo actividade de urato oxidase gene recombinante que a codifica vector de expressao portador deste gene microorganismos procariotas e celulas eucariotas transformados por este vector de expressao
CO4950591A1 (es) Gen quimerico que tiene un promotor dependiente de la luz, que confiere la tolerancia a los inhibidores de la mppd
ES2104596T3 (es) Nuevos polipeptidos, secuencias de adn que permiten su expresion, metodo de preparacion, y utilizacion.
ES2171465T3 (es) Inhibidor de la esterasa de c1 para reducir lesiones de miocardio durante un infarto de miocardio agudo.
ES2074280T3 (es) Metodo para modificar el tropismo de celula, tejido o huesped de un microorganismo, microorganismos recombinantes obtenidos de este modo y su uso en medicina y medicina veterinaria.
BR9803694A (pt) ácidos 6- e 7-aminotetraidroisoquinolinacarboxìlico substituìdos.
MX9200917A (es) Produccion de plantas resistentes a los ataques de sclerotinia sclerotiorum por introduccion de un gen que codifica una oxalato-oxidasa.

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 596812

Country of ref document: ES