ES2109194B1 - Un procedimiento para producir la enzima d-aminoacido oxidasa de rhodotorula gracilis en celulas hospedantes. - Google Patents

Un procedimiento para producir la enzima d-aminoacido oxidasa de rhodotorula gracilis en celulas hospedantes.

Info

Publication number
ES2109194B1
ES2109194B1 ES09600906A ES9600906A ES2109194B1 ES 2109194 B1 ES2109194 B1 ES 2109194B1 ES 09600906 A ES09600906 A ES 09600906A ES 9600906 A ES9600906 A ES 9600906A ES 2109194 B1 ES2109194 B1 ES 2109194B1
Authority
ES
Spain
Prior art keywords
amino acid
cells
acid oxidase
hospedant
producing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES09600906A
Other languages
English (en)
Other versions
ES2109194A1 (es
Inventor
Lopez Jose Luis Garcia
Rubio Estrella Cortes
Palacios Jorge Alonso
Fuente Jose Luis Barredo
Garcia Bruno Diez
Valle Miguel Angel Moreno
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Antibioticos SA
Original Assignee
Antibioticos SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Antibioticos SA filed Critical Antibioticos SA
Priority to ES09600906A priority Critical patent/ES2109194B1/es
Priority to US08/973,914 priority patent/US6187574B1/en
Priority to PCT/ES1997/000099 priority patent/WO1997040171A1/es
Priority to JP53776297A priority patent/JP3476834B2/ja
Priority to EP97919405A priority patent/EP0843015A1/en
Priority to AU23890/97A priority patent/AU2389097A/en
Publication of ES2109194A1 publication Critical patent/ES2109194A1/es
Application granted granted Critical
Publication of ES2109194B1 publication Critical patent/ES2109194B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0022—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
    • C12N9/0024—D-Amino acid oxidase (1.4.3.3)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

UN PROCEDIMIENTO PARA PRODUCIR LA ENZIMA D-AMINOACIDO OXIDASA DE RHODOTORULA GRACILIS EN CELULAS HOSPEDANTES. EL PROCEDIMIENTO DE EXPRESION DE LA ENZIMA COMPRENDE: AISLAR EL ADN COMPLEMENTARIO CORRESPONDIENTE AL ARN MENSAJERO DEL GEN QUE CODIFICA PARA LA D-AMINOACIDO OXIDASA DE CUALQUIER CEPA DE RHODOTORULA GRACILIS PRODUCTORA DE DICHA ENZIMA; FUSIONAR EL FRAGMENTO DE ADN QUE CODIFICA PARA LA D-AMINOACIDO OXIDASA DE RHODOTORULA GRACILIS A UNA SECUENCIA DE ADN QUE CONTENGA UN SITIO DE UNION AL RIBOSOMA Y UNA SECUENCIA PROMOTORA DE ALTA EFICACIA PARA LA EXPRESION DE GENES EN CELULAS HOSPEDANTES; INSERTAR EL FRAGMENTO DE ADN EN UN PLASMIDO DE APROPIADO PARA LA CELULA HOSPEDANTE; CULTIVAR LAS CELULAS HOSPEDANTES TRANSFORMADAS CON ESTE PLASMIDO; Y RECUPERAR LA ENZIMA.
ES09600906A 1996-04-22 1996-04-22 Un procedimiento para producir la enzima d-aminoacido oxidasa de rhodotorula gracilis en celulas hospedantes. Expired - Fee Related ES2109194B1 (es)

Priority Applications (6)

Application Number Priority Date Filing Date Title
ES09600906A ES2109194B1 (es) 1996-04-22 1996-04-22 Un procedimiento para producir la enzima d-aminoacido oxidasa de rhodotorula gracilis en celulas hospedantes.
US08/973,914 US6187574B1 (en) 1996-04-22 1997-04-18 Process for producing the enzyme D-amino acid oxidase of Rhodotorula gracilis in host cells
PCT/ES1997/000099 WO1997040171A1 (es) 1996-04-22 1997-04-18 Un procedimiento para producir la enzima d-aminoacido oxidasa de rhodotorula gracilis en celulas hospedantes
JP53776297A JP3476834B2 (ja) 1996-04-22 1997-04-18 宿主細胞においてロドトルラ・グラシリスの酵素ｄ−アミノ酸オキシダーゼを製造するための方法
EP97919405A EP0843015A1 (en) 1996-04-22 1997-04-18 PROCESS FOR PRODUCING THE ENZYME D-AMINOACIDO OXYDASE OF $i(RHODOTORULA GRACILIS) IN HOST CELLS
AU23890/97A AU2389097A (en) 1996-04-22 1997-04-18 Process for producing the enzyme d-aminoacido oxydase of (rhodotorula gracilis) in host cells

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES09600906A ES2109194B1 (es) 1996-04-22 1996-04-22 Un procedimiento para producir la enzima d-aminoacido oxidasa de rhodotorula gracilis en celulas hospedantes.

Publications (2)

Publication Number Publication Date
ES2109194A1 ES2109194A1 (es) 1998-01-01
ES2109194B1 true ES2109194B1 (es) 1998-07-16

Family

ID=8294567

Family Applications (1)

Application Number Title Priority Date Filing Date
ES09600906A Expired - Fee Related ES2109194B1 (es) 1996-04-22 1996-04-22 Un procedimiento para producir la enzima d-aminoacido oxidasa de rhodotorula gracilis en celulas hospedantes.

Country Status (6)

Country Link
US (1) US6187574B1 (es)
EP (1) EP0843015A1 (es)
JP (1) JP3476834B2 (es)
AU (1) AU2389097A (es)
ES (1) ES2109194B1 (es)
WO (1) WO1997040171A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2131018B1 (es) 1997-09-25 2000-04-01 Antibioticos Sau Un procedimiento enzimatico para preparar acido 7beta-(4-carboxibutanamido)cefalosporanico utilizando la enzima d-aminoacido oxidasa de trigonopsis variabilis modificada producida en escherichia coli.
GB0201043D0 (en) * 2002-01-17 2002-03-06 Swetree Genomics Ab Plants methods and means
EP1375649B1 (en) * 2002-06-19 2012-03-07 Evonik Degussa GmbH D-amino acid oxidase from Arthrobacter protophormiae
DE102004008445A1 (de) * 2004-02-19 2005-09-08 Degussa Ag Verfahren zur Herstellung von L-Aminosäuren aus D-Aminosäuren
WO2007015511A1 (ja) 2005-08-02 2007-02-08 Kaneka Corporation Ｄ－アミノ酸オキシダーゼ、およびｌ－アミノ酸、２－オキソ酸、又は環状イミンの製造方法。
EP2371946A1 (en) 2010-03-30 2011-10-05 Rijksuniversiteit Groningen Improved methods and host cells for the production and use of D-amino acid oxidase (DAO).
EP3277822B1 (en) * 2015-04-03 2020-05-06 Temasek Life Sciences Laboratory Limited D-amino acid-inducible gene expression system for rhodosporidium and rhodotorula
CN116376940A (zh) * 2023-03-09 2023-07-04 浙江新安化工集团股份有限公司 一种表达d-氨基酸氧化酶的方法及其应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2075056T3 (es) * 1988-10-13 1995-10-01 Fujisawa Pharmaceutical Co Oxidasa de los d-aminoacidos.
IT1252308B (it) * 1990-12-21 1995-06-08 Antibioticos Spa Procedimento enzimatico per la produzione di acido 7- amminocefalosporanico e derivati
IT1247964B (it) * 1991-06-03 1995-01-05 Ministero Dell Uni E Della Processo per la preparazione enzimatica di derivati cefalosporanici
CA2100987C (en) * 1992-07-27 1999-06-15 Kaoru Furuya A transformant capable of producing d-amino acid oxidase
IT1275909B1 (it) * 1995-03-07 1997-10-24 Mirella Pilone Frammento di dna codificante d-amminoacido ossidasi
US5877013A (en) * 1997-07-31 1999-03-02 Food Industry Research And Development Institute Rhodosporidium D-amino acid oxidase

Also Published As

Publication number Publication date
JPH11514241A (ja) 1999-12-07
ES2109194A1 (es) 1998-01-01
AU2389097A (en) 1997-11-12
JP3476834B2 (ja) 2003-12-10
WO1997040171A1 (es) 1997-10-30
EP0843015A1 (en) 1998-05-20
US6187574B1 (en) 2001-02-13

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