ES2109258T3 - Inmunoensayo optico de alta sensibilidad que utiliza reactivos marcados con enzimas. - Google Patents
Inmunoensayo optico de alta sensibilidad que utiliza reactivos marcados con enzimas.Info
- Publication number
- ES2109258T3 ES2109258T3 ES91308968T ES91308968T ES2109258T3 ES 2109258 T3 ES2109258 T3 ES 2109258T3 ES 91308968 T ES91308968 T ES 91308968T ES 91308968 T ES91308968 T ES 91308968T ES 2109258 T3 ES2109258 T3 ES 2109258T3
- Authority
- ES
- Spain
- Prior art keywords
- layer
- substrate
- enzyme
- analyte
- immuno assay
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108090000790 Enzymes Proteins 0.000 title abstract 3
- 102000004190 Enzymes Human genes 0.000 title abstract 3
- 238000003018 immunoassay Methods 0.000 title abstract 3
- 230000003287 optical effect Effects 0.000 title abstract 3
- 239000003153 chemical reaction reagent Substances 0.000 title 1
- 239000000758 substrate Substances 0.000 abstract 3
- 239000012491 analyte Substances 0.000 abstract 2
- 108010093096 Immobilized Enzymes Proteins 0.000 abstract 1
- 239000007795 chemical reaction product Substances 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 238000002405 diagnostic procedure Methods 0.000 abstract 1
- 239000003446 ligand Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 230000001376 precipitating effect Effects 0.000 abstract 1
- 238000001556 precipitation Methods 0.000 abstract 1
- 239000007787 solid Substances 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 239000010409 thin film Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
UN DISPOSITIVO PARA UN INMUNOENSAYO OPTICO DE PELICULA DELGADA QUE CONSTA DE UN SUSTRATO DE SOPORTE SOLIDO QUE TIENE UNA SUPERFICIE SUPERIOR Y OTRO INFERIOR Y, SOPORTANDO EN SU SUPERFICIE SUPERIOR, UNA CAPA DE ANTICUERPOS CON LIGANDOS NO ETIQUETADOS UNIDA A DICHO SUSTRATO, CONSTANDO AL MENOS UNA CAPA DE UN CONJUGADO DE ENZIMAS INMOVILIZADO, COMPLEJADO CON UNA MUESTRA DE ANALISIS DE INTERES Y CAPAZ DE INTERACTUAR ADEMAS CON UNA SUSTANCIA DE SUMINISTRO REACTIVA A LA ENCIMA PARA FORMAR UN PRODUCTO DE REACCION INSOLUBLE, TENIENDO DICHA CAPA CON CONJUGADO DE ENZIMAS Y DICHA CAPA CON LOS ANTICUERPOS NO ETIQUETADOS UN CAMBIO DE MASA AUMENTADO SENSIBLEMENTE, PUDIENDOSE PRECIPITAR DICHA MASA A BASE DE PRECIPITAR EL AGENTE APLICADO A MODO DE SUSTRATO SOBRE LA MISMA. LA INVENCION TAMBIEN PRESENTA UN PROCESO CORRESPONDIENTE PARA DETECTAR CUALQUIER MUESTRA DE ENSAYO EN UN MEDIO Y UN KIT PARA UN TEST DE DIAGNOSTICO PARA UN INMUNOENSAYO OPTICO DE PELICULA DELGADA.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP91308968A EP0546222B1 (en) | 1991-10-01 | 1991-10-01 | Highly sensitive optical immunoassay using enzyme-labeled reagents |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2109258T3 true ES2109258T3 (es) | 1998-01-16 |
Family
ID=8208408
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES91308968T Expired - Lifetime ES2109258T3 (es) | 1991-10-01 | 1991-10-01 | Inmunoensayo optico de alta sensibilidad que utiliza reactivos marcados con enzimas. |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0546222B1 (es) |
| AT (1) | ATE158080T1 (es) |
| CA (1) | CA2078897A1 (es) |
| DE (1) | DE69127628T2 (es) |
| ES (1) | ES2109258T3 (es) |
| HK (1) | HK1000771A1 (es) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0919811A1 (en) * | 1997-12-01 | 1999-06-02 | Universiteit Maastricht | Immunoassay method and kit |
| DE10222979B4 (de) * | 2002-05-23 | 2006-06-08 | Sartorius Ag | Verfahren zur Verstärkung eines Signals |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1025772A (en) * | 1972-06-26 | 1978-02-07 | Ivar Giaever | Method and apparatus for detection and purification of proteins and antibodies |
| SE416681B (sv) * | 1979-04-17 | 1981-01-26 | Johan Emanuel Stenberg | Sett att jemfora tva ytors reflexionsegenskaper |
| AU561066B2 (en) * | 1982-03-22 | 1987-04-30 | Biocarb Ab | Antibacterial composition containing an oligosaccharide |
| JP2534529B2 (ja) * | 1986-07-24 | 1996-09-18 | ブリティシュ・テレコミュニケ−ションズ・パブリック・リミテッド・カンパニ | 放射発生器 |
| EP0322549A3 (de) * | 1987-11-27 | 1991-05-08 | F. Hoffmann-La Roche Ag | Verfahren zur immunologischen Bestimmung von Polysaccharid-Antigenen aus Bakterien oder Pilzen in einer biologischen Probe |
| US4999284A (en) * | 1988-04-06 | 1991-03-12 | E. I. Du Pont De Nemours And Company | Enzymatically amplified piezoelectric specific binding assay |
| US5051356A (en) * | 1988-06-13 | 1991-09-24 | Eastman Kodak Company | Specific binding composition comprising a low pI protein or carbohydrate and a diagnostic test kit and method of use |
| GB8906776D0 (en) * | 1989-03-23 | 1989-05-10 | Amersham Int Plc | Assay method using surface plasmon resonance spectrometry |
| HK1000736A1 (en) * | 1989-09-18 | 1998-04-24 | Biostar Medical Products Incorporated | Method and apparatus for detection of an analyte |
| DE69010506T2 (de) * | 1989-10-04 | 1994-12-22 | Du Pont | Bestimmungsverfahren für biologische zielkomplexe auf der oberfläche eines biosensors. |
| GB8924951D0 (en) * | 1989-11-04 | 1989-12-28 | Fisons Plc | Device and methods |
| GB9018195D0 (en) * | 1990-08-18 | 1990-10-03 | Fisons Plc | Analytical device |
-
1991
- 1991-10-01 AT AT91308968T patent/ATE158080T1/de not_active IP Right Cessation
- 1991-10-01 DE DE69127628T patent/DE69127628T2/de not_active Expired - Fee Related
- 1991-10-01 ES ES91308968T patent/ES2109258T3/es not_active Expired - Lifetime
- 1991-10-01 EP EP91308968A patent/EP0546222B1/en not_active Expired - Lifetime
-
1992
- 1992-09-23 CA CA002078897A patent/CA2078897A1/en not_active Abandoned
-
1997
- 1997-12-02 HK HK97102309A patent/HK1000771A1/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| EP0546222B1 (en) | 1997-09-10 |
| CA2078897A1 (en) | 1993-04-02 |
| ATE158080T1 (de) | 1997-09-15 |
| DE69127628T2 (de) | 1998-01-22 |
| DE69127628D1 (de) | 1997-10-16 |
| HK1000771A1 (en) | 1998-04-24 |
| EP0546222A1 (en) | 1993-06-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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