ES2109716T3 - Deteccion de proteinas termoestables de carne de rumiantes. - Google Patents
Deteccion de proteinas termoestables de carne de rumiantes.Info
- Publication number
- ES2109716T3 ES2109716T3 ES94923006T ES94923006T ES2109716T3 ES 2109716 T3 ES2109716 T3 ES 2109716T3 ES 94923006 T ES94923006 T ES 94923006T ES 94923006 T ES94923006 T ES 94923006T ES 2109716 T3 ES2109716 T3 ES 2109716T3
- Authority
- ES
- Spain
- Prior art keywords
- pct
- detection
- date feb
- protein
- sec
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 5
- 102000004169 proteins and genes Human genes 0.000 title abstract 5
- 241000282849 Ruminantia Species 0.000 title 1
- 238000001514 detection method Methods 0.000 title 1
- 235000013372 meat Nutrition 0.000 title 1
- 239000000463 material Substances 0.000 abstract 2
- 108010010803 Gelatin Proteins 0.000 abstract 1
- 238000003556 assay Methods 0.000 abstract 1
- 238000010790 dilution Methods 0.000 abstract 1
- 239000012895 dilution Substances 0.000 abstract 1
- 229920000159 gelatin Polymers 0.000 abstract 1
- 239000008273 gelatin Substances 0.000 abstract 1
- 235000019322 gelatine Nutrition 0.000 abstract 1
- 235000011852 gelatine desserts Nutrition 0.000 abstract 1
- 238000003018 immunoassay Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/825—Pretreatment for removal of interfering factors from sample
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/108331—Preservative, buffer, anticoagulant or diluent
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Fire-Detection Mechanisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
SE SUMINISTRA UN METODO PARA CONCENTRAR PROTEINAS ESTABLES AL CALOR EN EXTRACTOS INMUNOGENICOS PREPARADOS A PARTIR DE MATERIALES DE ANIMALES, Y COMPUESTOS QUE LAS CONTIENEN, QUE INCREMENTAN DE ESTA FORMA LA SENSIBILIDAD DE PRUEBAS, TALES COMO INMUNOENSAYOS CUANDO INCORPORAN ESTE METODO PREPARATORIO COMO PASO PRELIMINAR. UN PRIMER ASPECTO DE LA INVENCION SUMINISTRA UN METODO PARA TRATAR PROTEINAS ESTABLES AL CALOR DERIVADAS DE UN MATERIAL ANIMAL ANTES DE REALIZAR UN ENSAYO PARA SU PRESENCIA, EL METODO COMPRENDE (A) LA PREPARACION DE UN EXTRACTO QUE CONTIENE LAS PROTEINAS DEL MATERIAL, (B) LA SEPARACION DE SUBSTANCIALMENTE TODA O DE PARTE DE LA GELATINA DEL EXTRACTO, PREFERIBLEMENTE CON PROTEINAS Y ACEITES VEGETALES, Y (C) LA CONCENTRACION DE LA PROTEINA RESTANTE DE MANERA QUE DE UNA COMPROBACION POSITIVA PARA LA PROTEINA. PREFERIBLEMENTE MEDIANTE UN INMUNOENSAYO A UNA DISOLUCION MAYOR DE 1 EN 6000 PARA CARNE Y HARINA DE HUESOS Y A 1 EN 400 PARA PREPARADOS ALIMENTICIOS DE LOS COMPUESTOS. EL METODO PARTICULARMENTE COMPRENDE LA PREPARACION DE UN EXTRACTO LIQUIDO, AMORTIGUADO DEL MATERIAL, LA SEPARACION DEL MATERIAL SOLIDO, POR EJEMPLO MEDIANTE FILTRADO, LA ADICION DE UNA SAL AL EXTRACTO A TAL CONCENTRACION QUE PROVOQUE UNA PRECIPITACION DIFERENCIAL DE LA GELATINA, LA SEPARACION DEL PRECIPITADO DE GELATINA DEL EXTRACTO, EL INCREMENTO DE LA CONCENTRACION DE LA SAL HASTA QUE UNA PARTE SUBSTANCIAL DEL RESTO DE LA PROTEINA SE PRECIPITE, Y EL AISLAMIENTO DE ESA PROTEINA. LAS SALES PREFERIDAS SON SALES DE AMONIO, PARTICULARMENTE SULFATO DE AMONIO. LA PRECIPITACION PREFERENCIAL DE LA GELATINA SE LLEVA A CABO DE FORMA CONVENIENTE MEDIANTE LA ADICION DE SUFICIENTE SAL AL EXTRACTO NEUTRALIZADO PARA SUMINISTRAR ENTRE UN 30% Y UN 70% DEL PESO DE SOLUCION DE SAL. PARA PRECIPITAR LAS PROTEINAS ESTABLES AL CALOR UNA VEZ QUE LA GELATINA HA SIDO SEPARADA SE AÑADE SULFATO DE SODIO ADICIONAL PARA DAR UNA CONCENTRACION FINAL QUE EXCEDA DEL 80% DEL PESO Y PREFERIBLEMENTE DEL 92.5% DEL PESO.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB939317199A GB9317199D0 (en) | 1993-08-18 | 1993-08-18 | Detection of ruminant proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2109716T3 true ES2109716T3 (es) | 1998-01-16 |
Family
ID=10740697
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES94923006T Expired - Lifetime ES2109716T3 (es) | 1993-08-18 | 1994-08-04 | Deteccion de proteinas termoestables de carne de rumiantes. |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US5910446A (es) |
| EP (1) | EP0714515B1 (es) |
| AT (1) | ATE160635T1 (es) |
| AU (1) | AU680528B2 (es) |
| DE (1) | DE69407051T2 (es) |
| DK (1) | DK0714515T3 (es) |
| ES (1) | ES2109716T3 (es) |
| GB (2) | GB9317199D0 (es) |
| GR (1) | GR3025616T3 (es) |
| WO (1) | WO1995005603A1 (es) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6919175B1 (en) * | 1995-04-01 | 2005-07-19 | Roche Diagnostics Gmbh | System for releasing and isolating nucleic acids |
| AU2002360991A1 (en) * | 2001-12-12 | 2003-07-09 | Antibodyshop A/S | Compositions, methods and kits for detection and quantification of plant and animal source contaminants |
| US20030129661A1 (en) * | 2001-12-18 | 2003-07-10 | Uttenthal Lars Otto | Compositions, methods and kits for detection and quantification of plant and animal source contaminants |
| BR0200690A (pt) * | 2002-03-04 | 2004-03-23 | Embrapa Pesquisa Agropecuaria | Método para detecção de proteinas de origem em misturas complexas |
| WO2004055494A2 (en) * | 2002-12-12 | 2004-07-01 | Strategic Diagnostics Inc. | Compositions and methods for detecting animal by product in feed |
| JP4624477B1 (ja) * | 2010-02-18 | 2011-02-02 | 田中貴金属工業株式会社 | 加工食品中の豚肉検出法およびその検出キット |
-
1993
- 1993-08-18 GB GB939317199A patent/GB9317199D0/en active Pending
-
1994
- 1994-08-04 GB GB9601756A patent/GB2296012A/en not_active Withdrawn
- 1994-08-04 AT AT94923006T patent/ATE160635T1/de not_active IP Right Cessation
- 1994-08-04 AU AU72718/94A patent/AU680528B2/en not_active Ceased
- 1994-08-04 US US08/596,189 patent/US5910446A/en not_active Expired - Fee Related
- 1994-08-04 EP EP94923006A patent/EP0714515B1/en not_active Expired - Lifetime
- 1994-08-04 ES ES94923006T patent/ES2109716T3/es not_active Expired - Lifetime
- 1994-08-04 WO PCT/GB1994/001715 patent/WO1995005603A1/en not_active Ceased
- 1994-08-04 DE DE69407051T patent/DE69407051T2/de not_active Expired - Fee Related
- 1994-08-04 DK DK94923006.4T patent/DK0714515T3/da active
-
1997
- 1997-12-05 GR GR970403267T patent/GR3025616T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| GB9317199D0 (en) | 1993-10-06 |
| EP0714515A1 (en) | 1996-06-05 |
| DE69407051D1 (de) | 1998-01-08 |
| GB2296012A (en) | 1996-06-19 |
| ATE160635T1 (de) | 1997-12-15 |
| DK0714515T3 (da) | 1998-03-30 |
| WO1995005603A1 (en) | 1995-02-23 |
| US5910446A (en) | 1999-06-08 |
| DE69407051T2 (de) | 1998-03-19 |
| GR3025616T3 (en) | 1998-03-31 |
| AU680528B2 (en) | 1997-07-31 |
| GB9601756D0 (en) | 1996-03-27 |
| EP0714515B1 (en) | 1997-11-26 |
| AU7271894A (en) | 1995-03-14 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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