ES2114049T3 - Procedimiento para detectar el oxido nitrico, los equivalentes del nitrosonio, los s-nitrosotioles y las n-nitrosoproteinas en los sistemas biologicos. - Google Patents

Procedimiento para detectar el oxido nitrico, los equivalentes del nitrosonio, los s-nitrosotioles y las n-nitrosoproteinas en los sistemas biologicos.

Info

Publication number
ES2114049T3
ES2114049T3 ES93910720T ES93910720T ES2114049T3 ES 2114049 T3 ES2114049 T3 ES 2114049T3 ES 93910720 T ES93910720 T ES 93910720T ES 93910720 T ES93910720 T ES 93910720T ES 2114049 T3 ES2114049 T3 ES 2114049T3
Authority
ES
Spain
Prior art keywords
nitric oxide
samples
nitrosonium
nitrosotiols
nitrosoproteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93910720T
Other languages
English (en)
Inventor
Jonathan Stamler
Joseph Loscalzo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Brigham and Womens Hospital Inc
Original Assignee
Brigham and Womens Hospital Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Brigham and Womens Hospital Inc filed Critical Brigham and Womens Hospital Inc
Application granted granted Critical
Publication of ES2114049T3 publication Critical patent/ES2114049T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/84—Preparation of the fraction to be distributed
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/631—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited using photolysis and investigating photolysed fragments
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/84—Preparation of the fraction to be distributed
    • G01N2030/8429—Preparation of the fraction to be distributed adding modificating material
    • G01N2030/8441—Preparation of the fraction to be distributed adding modificating material to modify physical properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/74—Optical detectors
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/17—Nitrogen containing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/17—Nitrogen containing
    • Y10T436/170769—N-Nitroso containing [e.g., nitrosamine, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/17—Nitrogen containing
    • Y10T436/176152—Total nitrogen determined
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/17—Nitrogen containing
    • Y10T436/177692—Oxides of nitrogen

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

EN MUESTRAS BIOLOGICAS PUEDEN DETECTARSE OXIDO NITRICO, NITROSONIUM, S-NITROSOTIOLES Y S-NITROSO-PROTEINAS, USANDO UN METODO QUE IMPLICA LA INYECCION DE MUESTRAS DENTRO DE UNA CELULA DE FOTOLISIS, CON ANTERIORIDAD A LA DETECCION DE QUIMIOLUMINISCENCIA GENERADA POR LA REACCION ENTRE EL OXIDO NITRICO Y EL OZONO. PARA DETECTAR S-NITROSOTIOLES Y S-NITROSO-PROTEINAS, SE SOMETEN PARTES ALICUOTAS SEPARADAS DE LAS MISMAS MUESTRAS A UN TRATAMIENTO PREVIO CON ION MERCURIOSO, Y UN AGENTE DE PRECIPITACION DE PROTEINAS, RESPECTIVAMENTE, Y SE COMPARAN LAS SEÑALES DEL OXIDO NITRICO RESULTANTE CON LAS GENERADAS POR LAS MUESTRAS NO TRATADAS. ESTE METODO ES UTIL PARA CONTROLAR LOS NIVELES DE LA BIOACTIVIDAD DEL OXIDO NITRICO EN LOS ESTADOS BIOLOGICOS NORMALES Y EN LOS DE ENFERMEDAD, TALES COMO SHOCK SEPTICO, ATEROSCLEROSIS, TROMBOSIS, HIPERHOMOCISTEINEMIA, HIPERTENSION PULMONAR, MALIGNIDAD, INFECCIONES Y TRASTORNOS DEL SISTEMA NERVIOSO CENTRAL.
ES93910720T 1992-04-22 1993-04-21 Procedimiento para detectar el oxido nitrico, los equivalentes del nitrosonio, los s-nitrosotioles y las n-nitrosoproteinas en los sistemas biologicos. Expired - Lifetime ES2114049T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/872,237 US5459076A (en) 1992-04-22 1992-04-22 Method for detecting nitric oxide, nitrosonium equivalents, S-nitrosothiols and S-nitroso-proteins in biological systems

Publications (1)

Publication Number Publication Date
ES2114049T3 true ES2114049T3 (es) 1998-05-16

Family

ID=25359132

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93910720T Expired - Lifetime ES2114049T3 (es) 1992-04-22 1993-04-21 Procedimiento para detectar el oxido nitrico, los equivalentes del nitrosonio, los s-nitrosotioles y las n-nitrosoproteinas en los sistemas biologicos.

Country Status (9)

Country Link
US (1) US5459076A (es)
EP (1) EP0591511B1 (es)
JP (1) JPH06510605A (es)
AT (1) ATE163090T1 (es)
AU (1) AU664276B2 (es)
CA (1) CA2112107C (es)
DE (1) DE69316818T2 (es)
ES (1) ES2114049T3 (es)
WO (1) WO1993021525A1 (es)

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Publication number Priority date Publication date Assignee Title
AU3071592A (en) * 1991-11-14 1993-06-15 Brigham And Women's Hospital Nitrosylation of protein sh groups and amino acid residues as a therapeutic modality
SE503876C2 (sv) 1994-11-30 1996-09-23 Kjell Alving Detektion av inflammatoriska tillstånd i tarmkanalen genom att mäta kväveoxidhalten i ett prov taget ur tarmlumen
US6627738B2 (en) * 1995-09-15 2003-09-30 Duke University No-modified hemoglobins and uses therefor
US6911427B1 (en) 1995-09-15 2005-06-28 Duke University No-modified hemoglobins and uses therefore
US5891735A (en) * 1995-09-15 1999-04-06 Duke University Medical Center Method for measuring nitric oxide in nitrosyl (FeII)-hemoglobin and S-nitrosohemoglobin
GB2314156A (en) * 1996-06-12 1997-12-17 Secr Defence Explosives detection
JP3013239B2 (ja) * 1997-04-17 2000-02-28 株式会社エイコム Ｓ−ニトロソ化合物検出方法
RU2147127C1 (ru) * 1998-07-14 2000-03-27 Иркутский государственный медицинский университет Минздрава РФ Способ дифференциальной диагностики анафилактического и анафилактоидного шока
DE19854808C2 (de) * 1998-11-27 2001-03-01 Sanol Arznei Schwarz Gmbh Detektionsverfahren
AU2002235545A1 (en) * 2000-08-25 2002-03-04 Queen Mary & Westfield College Assay for s-nitrosothiol compounds
US7419834B2 (en) * 2001-12-12 2008-09-02 University Of Massachusetts System for detection of nitrosylated proteins
US20060088942A1 (en) * 2002-05-29 2006-04-27 Stamler Jonathan S Measuring nitric oxide in blood gases and treatments based thereon
JP5179011B2 (ja) * 2002-10-09 2013-04-10 ディーエムアイ バイオサイエンシズ インコーポレイテッド 胎盤虚血および多臓器不全のモニタリング
US20050037518A1 (en) * 2003-08-12 2005-02-17 Stefano George B. Anti-inflammatory signal molecules and exercise
US7000560B2 (en) * 2003-12-11 2006-02-21 Honeywell International, Inc. Unmanned underwater vehicle docking station coupling system and method
WO2005081622A1 (en) * 2004-02-17 2005-09-09 Pharmacia & Upjohn Company Llc Methods and compositions for the detection of nitric oxide
WO2006094185A2 (en) * 2005-03-03 2006-09-08 Dmi Biosciences Inc. Quantification of proteins
AU2008279755B2 (en) * 2007-07-26 2014-01-09 Duke University Measuring amount of bound and combined nitric oxide in blood
US9651530B2 (en) * 2013-01-14 2017-05-16 Lifehealth, Llc Methods and apparatus for measuring nitric oxide in fluid
US9753019B2 (en) * 2013-01-14 2017-09-05 Lifehealth, Llc Measuring nitric oxide in fluid

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US3973910A (en) * 1973-02-05 1976-08-10 Thermo Electron Corporation Method of measuring the N-nitrosoamine content of a sample
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CA1307480C (en) * 1985-07-10 1992-09-15 Nanibhushan Dattagupta Prolonged chemiluminescence
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Also Published As

Publication number Publication date
DE69316818T2 (de) 1998-07-16
AU4111493A (en) 1993-11-18
JPH06510605A (ja) 1994-11-24
US5459076A (en) 1995-10-17
WO1993021525A1 (en) 1993-10-28
CA2112107C (en) 2002-06-25
EP0591511A1 (en) 1994-04-13
AU664276B2 (en) 1995-11-09
CA2112107A1 (en) 1993-10-28
EP0591511A4 (en) 1994-08-17
DE69316818D1 (de) 1998-03-12
ATE163090T1 (de) 1998-02-15
EP0591511B1 (en) 1998-02-04

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