ES2115553A1 - Metodo para detectar la presencia de polimorfismos de dna, estuche de ensayo y acidos nucleicos recombinantes correspondientes. - Google Patents
Metodo para detectar la presencia de polimorfismos de dna, estuche de ensayo y acidos nucleicos recombinantes correspondientes.Info
- Publication number
- ES2115553A1 ES2115553A1 ES09602239A ES9602239A ES2115553A1 ES 2115553 A1 ES2115553 A1 ES 2115553A1 ES 09602239 A ES09602239 A ES 09602239A ES 9602239 A ES9602239 A ES 9602239A ES 2115553 A1 ES2115553 A1 ES 2115553A1
- Authority
- ES
- Spain
- Prior art keywords
- dna
- test kit
- recombinant nucleic
- detecting
- nucleic acids
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title abstract 5
- 108020004707 nucleic acids Proteins 0.000 title abstract 3
- 102000039446 nucleic acids Human genes 0.000 title abstract 3
- 150000007523 nucleic acids Chemical class 0.000 title abstract 3
- 102000054765 polymorphisms of proteins Human genes 0.000 title abstract 2
- 101710095339 Apolipoprotein E Proteins 0.000 abstract 2
- 108091034117 Oligonucleotide Proteins 0.000 abstract 2
- 108091008146 restriction endonucleases Proteins 0.000 abstract 2
- 208000024827 Alzheimer disease Diseases 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 238000003745 diagnosis Methods 0.000 abstract 1
- 230000029087 digestion Effects 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
METODO PARA DETECTAR LA PRESENCIA DE POLIMORFISMOS DE DNA, ESTUCHE DE ENSAYO Y ACIDOS NUCLEICOS RECOMBINANTES CORRESPONDIENTES. EL METODO CONSISTE EN ANALIZAR DNA GENOMICO HUMANO, DNA GENOMICO CLONADO O DNA GENOMICO AMPLIFICADO MEDIANTE EL EMPLEO DE MOLECULAS DE OLIGONUCLEOTIDOS COMO SONDAS QUE HIBRIDAN CON UNA PORCION DEL GEN DE LA APOLIPOPROTEINA E. EN OTRA VARIANTE, LA DETECCION SE REALIZA MEDIANTE LA DIGESTION DEL DNA CON UNA O VARIAS ENZIMAS DE RESTRICCION, O BIEN MEDIANTE LA UTILIZACION DE DIFERENTES TECNICAS ELECTROFORETICAS. EL ESTUCHE DE ENSAYO SE CARACTERIZA POR UNA CANTIDAD DEFINIDA DE UNA O MAS SONDAS DE OLIGONUCLEOTIDOS MARCADOS, UNA CANTIDAD DEFINIDA DE UNA O MAS ENZIMAS DE RESTRICCION Y MEDIOS NECESARIOS PARA LA AMPLIFIACION DEL DNA. EL ACIDO NUCLEICO RECOMBINANTE A UTILIZAR EN EL METODO CONTIENE CUALQUIER FRAGMENTO DEL PROMOTOR DEL GEN DE LA APOLIPOPROTEINAEQUE INCLUYE UNA SERIE DE NUCLEOTIDOS DETERMINADOS DEL MENCIONADO GEN. LA INVENCION ES APLICABLE A LA DIAGNOSIS Y TERAPIA DE LA ENFERMEDAD DE ALZHEIMER.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES09602239A ES2115553B1 (es) | 1996-10-23 | 1996-10-23 | Metodo para detectar la presencia de polimorfismos de dna, estuche de ensayo y acidos nucleicos recombinantes correspondientes. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES09602239A ES2115553B1 (es) | 1996-10-23 | 1996-10-23 | Metodo para detectar la presencia de polimorfismos de dna, estuche de ensayo y acidos nucleicos recombinantes correspondientes. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2115553A1 true ES2115553A1 (es) | 1998-06-16 |
| ES2115553B1 ES2115553B1 (es) | 1999-02-16 |
Family
ID=8296459
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES09602239A Expired - Fee Related ES2115553B1 (es) | 1996-10-23 | 1996-10-23 | Metodo para detectar la presencia de polimorfismos de dna, estuche de ensayo y acidos nucleicos recombinantes correspondientes. |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES2115553B1 (es) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2142248A1 (es) * | 1997-11-20 | 2000-04-01 | Univ Madrid Autonoma | Polimorfismos en el promotor del gen humano de la apolipoproteina e, y sus usos para aplicaciones diagnosticas y terapeuticas. |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994009155A1 (en) * | 1992-10-13 | 1994-04-28 | Duke University | Methods of detecting alzheimer's disease |
| WO1995016791A1 (en) * | 1993-12-15 | 1995-06-22 | Mcgill University | Apolipoprotein e polymorphism and alzheimer's disease |
| WO1995029257A2 (en) * | 1994-04-27 | 1995-11-02 | Mcgill University | Apolipoprotein e polymorphism and treatment of alzheimer's disease |
-
1996
- 1996-10-23 ES ES09602239A patent/ES2115553B1/es not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994009155A1 (en) * | 1992-10-13 | 1994-04-28 | Duke University | Methods of detecting alzheimer's disease |
| WO1995016791A1 (en) * | 1993-12-15 | 1995-06-22 | Mcgill University | Apolipoprotein e polymorphism and alzheimer's disease |
| WO1995029257A2 (en) * | 1994-04-27 | 1995-11-02 | Mcgill University | Apolipoprotein e polymorphism and treatment of alzheimer's disease |
Non-Patent Citations (1)
| Title |
|---|
| MUI, S. et al., "A newly identified polymorphism in the apoli- protein E enhancer gene region is associated with Alzheimer's disease and strongly with the E4 allele", NEUROLOGY, 1996 julio, Vol. 47, pßgs. 196-201. *Ver el documento completo* * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2142248A1 (es) * | 1997-11-20 | 2000-04-01 | Univ Madrid Autonoma | Polimorfismos en el promotor del gen humano de la apolipoproteina e, y sus usos para aplicaciones diagnosticas y terapeuticas. |
Also Published As
| Publication number | Publication date |
|---|---|
| ES2115553B1 (es) | 1999-02-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EC2A | Search report published |
Date of ref document: 19980616 Kind code of ref document: A1 Effective date: 19980616 |
|
| PC2A | Transfer of patent | ||
| FD2A | Announcement of lapse in spain |
Effective date: 20170130 |