ES2115553A1 - Metodo para detectar la presencia de polimorfismos de dna, estuche de ensayo y acidos nucleicos recombinantes correspondientes. - Google Patents

Metodo para detectar la presencia de polimorfismos de dna, estuche de ensayo y acidos nucleicos recombinantes correspondientes.

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Publication number
ES2115553A1
ES2115553A1 ES09602239A ES9602239A ES2115553A1 ES 2115553 A1 ES2115553 A1 ES 2115553A1 ES 09602239 A ES09602239 A ES 09602239A ES 9602239 A ES9602239 A ES 9602239A ES 2115553 A1 ES2115553 A1 ES 2115553A1
Authority
ES
Spain
Prior art keywords
dna
test kit
recombinant nucleic
detecting
nucleic acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES09602239A
Other languages
English (en)
Other versions
ES2115553B1 (es
Inventor
Amate Fernando Valdivieso
Gomez-Heras Maria Jesu Bullido
Gonzalez Maria Jesus Artiga
Vicente Maria Recuero
Merlin Isabel Sastre
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boehringer Ingelheim España SA
Original Assignee
Boehringer Ingelheim España SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim España SA filed Critical Boehringer Ingelheim España SA
Priority to ES09602239A priority Critical patent/ES2115553B1/es
Publication of ES2115553A1 publication Critical patent/ES2115553A1/es
Application granted granted Critical
Publication of ES2115553B1 publication Critical patent/ES2115553B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Abstract

METODO PARA DETECTAR LA PRESENCIA DE POLIMORFISMOS DE DNA, ESTUCHE DE ENSAYO Y ACIDOS NUCLEICOS RECOMBINANTES CORRESPONDIENTES. EL METODO CONSISTE EN ANALIZAR DNA GENOMICO HUMANO, DNA GENOMICO CLONADO O DNA GENOMICO AMPLIFICADO MEDIANTE EL EMPLEO DE MOLECULAS DE OLIGONUCLEOTIDOS COMO SONDAS QUE HIBRIDAN CON UNA PORCION DEL GEN DE LA APOLIPOPROTEINA E. EN OTRA VARIANTE, LA DETECCION SE REALIZA MEDIANTE LA DIGESTION DEL DNA CON UNA O VARIAS ENZIMAS DE RESTRICCION, O BIEN MEDIANTE LA UTILIZACION DE DIFERENTES TECNICAS ELECTROFORETICAS. EL ESTUCHE DE ENSAYO SE CARACTERIZA POR UNA CANTIDAD DEFINIDA DE UNA O MAS SONDAS DE OLIGONUCLEOTIDOS MARCADOS, UNA CANTIDAD DEFINIDA DE UNA O MAS ENZIMAS DE RESTRICCION Y MEDIOS NECESARIOS PARA LA AMPLIFIACION DEL DNA. EL ACIDO NUCLEICO RECOMBINANTE A UTILIZAR EN EL METODO CONTIENE CUALQUIER FRAGMENTO DEL PROMOTOR DEL GEN DE LA APOLIPOPROTEINAEQUE INCLUYE UNA SERIE DE NUCLEOTIDOS DETERMINADOS DEL MENCIONADO GEN. LA INVENCION ES APLICABLE A LA DIAGNOSIS Y TERAPIA DE LA ENFERMEDAD DE ALZHEIMER.
ES09602239A 1996-10-23 1996-10-23 Metodo para detectar la presencia de polimorfismos de dna, estuche de ensayo y acidos nucleicos recombinantes correspondientes. Expired - Fee Related ES2115553B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES09602239A ES2115553B1 (es) 1996-10-23 1996-10-23 Metodo para detectar la presencia de polimorfismos de dna, estuche de ensayo y acidos nucleicos recombinantes correspondientes.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES09602239A ES2115553B1 (es) 1996-10-23 1996-10-23 Metodo para detectar la presencia de polimorfismos de dna, estuche de ensayo y acidos nucleicos recombinantes correspondientes.

Publications (2)

Publication Number Publication Date
ES2115553A1 true ES2115553A1 (es) 1998-06-16
ES2115553B1 ES2115553B1 (es) 1999-02-16

Family

ID=8296459

Family Applications (1)

Application Number Title Priority Date Filing Date
ES09602239A Expired - Fee Related ES2115553B1 (es) 1996-10-23 1996-10-23 Metodo para detectar la presencia de polimorfismos de dna, estuche de ensayo y acidos nucleicos recombinantes correspondientes.

Country Status (1)

Country Link
ES (1) ES2115553B1 (es)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2142248A1 (es) * 1997-11-20 2000-04-01 Univ Madrid Autonoma Polimorfismos en el promotor del gen humano de la apolipoproteina e, y sus usos para aplicaciones diagnosticas y terapeuticas.

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994009155A1 (en) * 1992-10-13 1994-04-28 Duke University Methods of detecting alzheimer's disease
WO1995016791A1 (en) * 1993-12-15 1995-06-22 Mcgill University Apolipoprotein e polymorphism and alzheimer's disease
WO1995029257A2 (en) * 1994-04-27 1995-11-02 Mcgill University Apolipoprotein e polymorphism and treatment of alzheimer's disease

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994009155A1 (en) * 1992-10-13 1994-04-28 Duke University Methods of detecting alzheimer's disease
WO1995016791A1 (en) * 1993-12-15 1995-06-22 Mcgill University Apolipoprotein e polymorphism and alzheimer's disease
WO1995029257A2 (en) * 1994-04-27 1995-11-02 Mcgill University Apolipoprotein e polymorphism and treatment of alzheimer's disease

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MUI, S. et al., "A newly identified polymorphism in the apoli- protein E enhancer gene region is associated with Alzheimer's disease and strongly with the E4 allele", NEUROLOGY, 1996 julio, Vol. 47, pßgs. 196-201. *Ver el documento completo* *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2142248A1 (es) * 1997-11-20 2000-04-01 Univ Madrid Autonoma Polimorfismos en el promotor del gen humano de la apolipoproteina e, y sus usos para aplicaciones diagnosticas y terapeuticas.

Also Published As

Publication number Publication date
ES2115553B1 (es) 1999-02-16

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