ES2116229B1 - Metodo de diferenciacion bacteriana basado en la determinacion de actividades aminopeptidasicas. - Google Patents

Metodo de diferenciacion bacteriana basado en la determinacion de actividades aminopeptidasicas.

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Publication number
ES2116229B1
ES2116229B1 ES9601613A ES9601613A ES2116229B1 ES 2116229 B1 ES2116229 B1 ES 2116229B1 ES 9601613 A ES9601613 A ES 9601613A ES 9601613 A ES9601613 A ES 9601613A ES 2116229 B1 ES2116229 B1 ES 2116229B1
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ES
Spain
Prior art keywords
activities
differentiation method
aminopeptidasic
determination
method based
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES9601613A
Other languages
English (en)
Other versions
ES2116229A1 (es
Inventor
Vilardel Fidel Toldra
Herranz Yolanda Sanz
Aguilar Rafael Vila
Casino Amparo Feria
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior de Investigaciones Cientificas CSIC filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES9601613A priority Critical patent/ES2116229B1/es
Publication of ES2116229A1 publication Critical patent/ES2116229A1/es
Application granted granted Critical
Publication of ES2116229B1 publication Critical patent/ES2116229B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Abstract

METODO DE DIFERENCIACION BACTERIANA BASADO EN LA DETERMINACION DE ACTIVIDADES AMINOPEPTIDASICAS. EL METODO DE DIFERENCIACION BACTERIANA ESTA CARACTERIZADO POR LA PREPARACION DE UNA SUSPENSION CELULAR A PARTIR DE UN CULTIVO ACTIVO Y POSTERIOR DETECCION DE LAS ACTIVIDADES ENZIMATICAS AMONOPEPTIDASICAS MEDIANTE SUSTRATOS FLUORESCENTES, MEDIANTE LAS ETAPAS SIGUIENTES: PREPARACION DE LA SUSPENSION CELULAR, PREPARACION DE SUSTRATOS ESPECIFICOS Y MEDIDA FLUORESCENTE DE LAS ACTIVIDADES ENZIMATICAS. ESTE PROCEDIMIENTO PERMITE DISTINGUIR ESPECIES BACTERIANAS Y SELECCIONAR CEPAS. LAS VENTAJAS SON LA SIMPLICIDAD DE LA INSTRUMENTACION NECESARIA, RAPIDEZ Y SENSIBILIDAD. SU UTILIZACION EN LA INDUSTRIA ALIMENTARIA, FARMACEUTICA Y CLINICA.
ES9601613A 1996-07-19 1996-07-19 Metodo de diferenciacion bacteriana basado en la determinacion de actividades aminopeptidasicas. Expired - Fee Related ES2116229B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9601613A ES2116229B1 (es) 1996-07-19 1996-07-19 Metodo de diferenciacion bacteriana basado en la determinacion de actividades aminopeptidasicas.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9601613A ES2116229B1 (es) 1996-07-19 1996-07-19 Metodo de diferenciacion bacteriana basado en la determinacion de actividades aminopeptidasicas.

Publications (2)

Publication Number Publication Date
ES2116229A1 ES2116229A1 (es) 1998-07-01
ES2116229B1 true ES2116229B1 (es) 1999-03-01

Family

ID=8295551

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9601613A Expired - Fee Related ES2116229B1 (es) 1996-07-19 1996-07-19 Metodo de diferenciacion bacteriana basado en la determinacion de actividades aminopeptidasicas.

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ES (1) ES2116229B1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5636189B2 (ja) 2006-10-27 2014-12-03 ライフ テクノロジーズ コーポレーション 蛍光発生性pH感受性色素およびその使用方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2671561B1 (fr) * 1991-01-16 1993-03-26 Bio Merieux Procede et milieu d'identification de bacteries du genre listeria.
WO1994021816A1 (en) * 1993-03-25 1994-09-29 Envirocon International Incorporated Test kits and methods for rapidly testing for contamination by microorganisms

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Publication number Publication date
ES2116229A1 (es) 1998-07-01

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