ES2120409T3 - Nuevos vectores de expresion y metodos para la produccion de proteinas intracelulares. - Google Patents

Nuevos vectores de expresion y metodos para la produccion de proteinas intracelulares.

Info

Publication number
ES2120409T3
ES2120409T3 ES90902632T ES90902632T ES2120409T3 ES 2120409 T3 ES2120409 T3 ES 2120409T3 ES 90902632 T ES90902632 T ES 90902632T ES 90902632 T ES90902632 T ES 90902632T ES 2120409 T3 ES2120409 T3 ES 2120409T3
Authority
ES
Spain
Prior art keywords
methods
production
expression vectors
recombinant dna
dna molecules
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90902632T
Other languages
English (en)
Inventor
Ilkka Palva
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SPECTRUM MEDICAL SCIENCES Ltd
Original Assignee
SPECTRUM MEDICAL SCIENCES Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SPECTRUM MEDICAL SCIENCES Ltd filed Critical SPECTRUM MEDICAL SCIENCES Ltd
Application granted granted Critical
Publication of ES2120409T3 publication Critical patent/ES2120409T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/235—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bordetella (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12N9/1033—Chloramphenicol O-acetyltransferase (2.3.1.28)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

LA INVENCION SE REFIERE A MOLECULAS DE ADN RECOMBINANTES Y A METODOS PARA PRODUCIR PROTEINAS MEDIANTE DICHAS MOLECULAS. EN PARTICULAR, LA PRESENTE INVENCION SE REFIERE A MOLECULAS RECOMBINANTES QUE PUEDEN SER SINTETIZADAS EN MOLECULA DE DEFORMACION BACILLUS QUE COMPRENDE LA REGULACION Y LA SECUENCIA DE SEÑAL NO FUNCIONAL SUPRIMIDA DEL GEN A-AMILASA DE B.AMILOLIQUEFACIENS, O UNA PARTE SUSTANCIAL DE ESTE, EN CUYA SECUENCIA SE PUEDE UNIR UN GEN ESTRUCTURAL DE UNA PROTEINA HETEROLOGO U HOMOLOGA DESEADA O UN PEPTIDO. ESTAS MOLECULAS DE ADN RECOMBINANTE PUEDEN USARSE, POR EJEMPLO, PARA LLEVAR A CABO UNA EXPRESION INTRACELULAR DE CUALQUIER PROTEINA O PEPTIDO DESEADO EN LA BACTERIA DE DEFORMACION BACILLUS
ES90902632T 1989-02-10 1990-02-08 Nuevos vectores de expresion y metodos para la produccion de proteinas intracelulares. Expired - Lifetime ES2120409T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US30886189A 1989-02-10 1989-02-10

Publications (1)

Publication Number Publication Date
ES2120409T3 true ES2120409T3 (es) 1998-11-01

Family

ID=23195696

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90902632T Expired - Lifetime ES2120409T3 (es) 1989-02-10 1990-02-08 Nuevos vectores de expresion y metodos para la produccion de proteinas intracelulares.

Country Status (7)

Country Link
EP (1) EP0456698B1 (es)
AT (1) ATE167897T1 (es)
AU (1) AU649606B2 (es)
DE (1) DE69032453T2 (es)
DK (1) DK0456698T3 (es)
ES (1) ES2120409T3 (es)
WO (1) WO1990009448A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6153398A (en) * 1997-12-24 2000-11-28 The University Of Chicago Method to identify specific inhibitors of IMP dehydrogenase
CN118434872A (zh) 2021-12-20 2024-08-02 巴斯夫欧洲公司 用于提高芽孢杆菌中胞内蛋白质产量的方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI64813C (fi) * 1980-12-31 1984-01-10 Ilkka Antero Palva Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer
CA1340373C (en) * 1986-01-28 1999-02-02 Rino Rappuoli Cloning and sequencing of the dna fragment which codes for the five subunits of the pertussis toxin, a hybrid plasmid containing the dna fragment and micro-organisms transformed by the hybrid plasmid and capable of expressing all or some of the subunits of the pertussis toxin
IT1202612B (it) * 1987-03-03 1989-02-09 Eniricerche Spa Plasmidi ricombinanti utili per l'espressione in bacillus di proteine eterologhe

Also Published As

Publication number Publication date
EP0456698B1 (en) 1998-07-01
ATE167897T1 (de) 1998-07-15
DE69032453D1 (de) 1998-08-06
AU5034490A (en) 1990-09-05
WO1990009448A1 (en) 1990-08-23
AU649606B2 (en) 1994-06-02
DK0456698T3 (da) 1999-04-12
EP0456698A1 (en) 1991-11-21
DE69032453T2 (de) 1999-03-18

Similar Documents

Publication Publication Date Title
FR19C1020I1 (fr) Nouvelles molecules b7-4 et leurs utilisations
DK1218504T3 (da) GL50 molekyler og anvendelser deraf
IE883612L (en) Vectors and compounds for direct expression of activated human protein c
EA199700244A1 (ru) Слитый белок, экспрессирующий вектор, рекомбинантный штамм, вакцинный препарат
WO2005017149A8 (en) Compositions and methods for enhanced expression of recombinant polypeptides from a single vector using a peptide cleavage site
WO2002099070A3 (en) Compositions and methods for high-level, large-scale production of recombinant proteins
SE8107812L (sv) Rekombinerade dna-molekyler och forfaranden for framstellning av proteiner
ATE380867T1 (de) Lactoferrinrezeptorgen von moraxella
ATE419358T1 (de) Gebrauchsverfaheren eines neuen lysyloxidase- verwandten proteins
DE69837085D1 (de) Crsp-proteine (cystein-reiche, sekretierte proteine), die dafür kodierenden nukleinsäuren und ihre verwendungen
TR27970A (tr) Bitki kofulu icine hedeflemede kullanilan yeni sinyal dizimleri.
ATE353971T1 (de) Verbesserte prokariontische proteinenexpression
NO930428L (no) Proteinstrukturen til plantetoksingelonin
DK0456698T3 (da) Hidtil ukendte ekspressionsvektorer og fremgangsmåder til intracellulær proteinekspression
WO1995008628A3 (en) Nucleic acids encoding merosin, merosin fragments and uses thereof
ATE295890T1 (de) Vektor zur expression von n-terminal verlängerten proteinen in hefezellen
KR880701073A (ko) 제조합 dna 기법에 의해 생성된 민악티빈
IT8521507A0 (it) Vettore di clonaggio, molecole di dna ricombinante, ceppi di bacillus subtilis trasformati con dette molecole e metodi per l'espressione di geni eterologhi e produzione e secrezione di proteine codificate da detti geni.
Palva Expression vectors and methods for intracellular protein production in bascillus
AU2002234132A1 (en) 13245, a novel human myotonic dystrophy type protein kinase and uses therefor
ECSP993285A (es) Sintesis recombinante de beta lipotropina y otros peptidos
DK135387A (da) Fremgangsmaade til fremstilling af proteiner i aspergillus oryzae og promoter til anvendelse i aspergillus
ATE476194T1 (de) Ein mitglied der menschlichen proteinkinasefamilie, und verwendungen dafür
DE69939989D1 (de) Menschliches gas-6 (growth arrest-specific gene 6) Protein und dafür kodierende Nukleinsäuren
ATE93893T1 (de) Expressionssysteme zur ueberproduktion gewuenschter proteine.

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 456698

Country of ref document: ES