ES2129482T3 - Anticuerpos monoclonales que aglutinan los basofilos, procedimiento de separacion de basofilos, procedimiento para liberar mediadores quimicos de basofilos y procedimiento para comprobar la liberacion de mediadores quimicos derivados de basofilos. - Google Patents

Anticuerpos monoclonales que aglutinan los basofilos, procedimiento de separacion de basofilos, procedimiento para liberar mediadores quimicos de basofilos y procedimiento para comprobar la liberacion de mediadores quimicos derivados de basofilos.

Info

Publication number
ES2129482T3
ES2129482T3 ES93117830T ES93117830T ES2129482T3 ES 2129482 T3 ES2129482 T3 ES 2129482T3 ES 93117830 T ES93117830 T ES 93117830T ES 93117830 T ES93117830 T ES 93117830T ES 2129482 T3 ES2129482 T3 ES 2129482T3
Authority
ES
Spain
Prior art keywords
procedure
basophils
chemical
release
monoclonal antibodies
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93117830T
Other languages
English (en)
Inventor
Shinji Nishimura
Hiroshi Nishi
Masaji Nishimura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shionogi and Co Ltd
Original Assignee
Shionogi and Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shionogi and Co Ltd filed Critical Shionogi and Co Ltd
Application granted granted Critical
Publication of ES2129482T3 publication Critical patent/ES2129482T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C07K14/4705—Regulators; Modulating activity stimulating, promoting or activating activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0642—Granulocytes, e.g. basopils, eosinophils, neutrophils, mast cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C12N5/163—Animal cells one of the fusion partners being a B or a T lymphocyte
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Physiology (AREA)
  • Virology (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

LOS ANTICUERPOS MONOCLONALES DE LA INVENCION HACEN POSIBLE SEPARAR BASOFILOS ADECUADOS PARA LA PRUEBA DE LIBERACION DE MEDIADORES QUIMICOS ESPECIFICOS INTERMEDIADOS POR IGE, A CAUSA DE QUE RETIENE SU REACTIVIDAD CON LOS BASOFILOS INCLUSO DESPUES DE SER INMOVILIZADO SOBRE UN PORTADOR SOLIDO, Y ACUSA DE QUE NO INHIBEN LA LIBERACION DE LOS MEDIADORES QUIMICOS INDUCIDOS POR ALERGENOS O ANTICUERPOS ANTIIGE, Y NO INDUCEN LA LIBERACION NO ESPECIFICA DE MEDIADORES QUIMICOS. TAMBIEN, EL METODO PARA SEPARAR LOS BASOFILOS DE LA INVENCION SIMPLIFICA LA SEPARACION DE LOS BASOFILOS DE LA SANGRE, Y MEDIANTE EL USO DE ESTE METODO, LA PRUEBA DE LIBERACION DE ISTAMINA QUE DE OTRA FORMA REQUIERE LOS PROCEDIMIENTOS COMPLEJOS PUEDE SIMPLIFICARSE. ADEMAS, EL GRUPO DE CELULAS OBTENIDAS POR EL METODO PARA SEPARAR BASOFILOS DE LA INVENCION, PUEDE UTILIZARSE FACILMENTE EN LAS PRUEBAS DE LIBERACION PARA MEDIADORES QUIMICOS LIBERADOS A PARTIR DE LOS BASOFILOS TALES COMO EL LEUCOTRIENO Y EL PAF, QUE DE OTRA FORMA REQUIEREN UN MANEJO EXPERTO.
ES93117830T 1992-11-04 1993-11-03 Anticuerpos monoclonales que aglutinan los basofilos, procedimiento de separacion de basofilos, procedimiento para liberar mediadores quimicos de basofilos y procedimiento para comprobar la liberacion de mediadores quimicos derivados de basofilos. Expired - Lifetime ES2129482T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP32116492 1992-11-04

Publications (1)

Publication Number Publication Date
ES2129482T3 true ES2129482T3 (es) 1999-06-16

Family

ID=18129523

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93117830T Expired - Lifetime ES2129482T3 (es) 1992-11-04 1993-11-03 Anticuerpos monoclonales que aglutinan los basofilos, procedimiento de separacion de basofilos, procedimiento para liberar mediadores quimicos de basofilos y procedimiento para comprobar la liberacion de mediadores quimicos derivados de basofilos.

Country Status (9)

Country Link
US (1) US5500348A (es)
EP (1) EP0596479B1 (es)
JP (1) JP3550410B2 (es)
KR (1) KR100280239B1 (es)
AT (1) ATE176930T1 (es)
DE (1) DE69323589T2 (es)
DK (1) DK0596479T3 (es)
ES (1) ES2129482T3 (es)
TW (1) TW378213B (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6352694B1 (en) * 1994-06-03 2002-03-05 Genetics Institute, Inc. Methods for inducing a population of T cells to proliferate using agents which recognize TCR/CD3 and ligands which stimulate an accessory molecule on the surface of the T cells
US7479269B2 (en) * 1988-11-23 2009-01-20 Genetics Institute, Llc Methods for selectively enriching TH1 and TH2 cells
US6534055B1 (en) * 1988-11-23 2003-03-18 Genetics Institute, Inc. Methods for selectively stimulating proliferation of T cells
US6905680B2 (en) * 1988-11-23 2005-06-14 Genetics Institute, Inc. Methods of treating HIV infected subjects
US7175843B2 (en) * 1994-06-03 2007-02-13 Genetics Institute, Llc Methods for selectively stimulating proliferation of T cells
US5722470A (en) * 1995-11-09 1998-03-03 Glaxo Group Limited Bead dispensing device and methods
DE19643427C1 (de) * 1996-10-22 1998-02-12 Bundesrep Deutschland Verfahren zur Bestimmung von Allergenen in der Luft
FR2765341A1 (fr) * 1997-06-25 1998-12-31 Alain Funes Methode pour l'analyse de l'activation des basophiles humains par mesure de l'expression membranaire du marqueur cd63
US6151973A (en) * 1999-01-29 2000-11-28 Glaxo Wellcome Inc. Bead picking apparatus and method
EP1179179B1 (de) * 1999-05-19 2004-10-20 Eberhard-Karls-Universität Tübingen Universitätsklinikum Verwendung eines antikörpers zur detektion von basophilen und/oder mastzellen
CA2412994A1 (en) * 2000-06-30 2002-12-27 Kyowa Medex Co., Ltd. Insoluble carrier particle nephelometric immunoassay reagent
WO2002010760A1 (fr) * 2000-07-27 2002-02-07 Kyowa Medex Co., Ltd Technique relative a une epreuve d'immunite menee avec des particules d'un vehicule insoluble et reactif a cet effet
US20030022250A1 (en) * 2001-05-29 2003-01-30 Dreskin Stephen C. Functional IgE test methods and compositions
US8242874B2 (en) * 2005-08-23 2012-08-14 Lear Corporation Electrical connector housing
CN112415191A (zh) * 2020-11-06 2021-02-26 郑州人福博赛生物技术有限责任公司 一种白细胞三烯检测试剂盒

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4559310A (en) * 1983-05-20 1985-12-17 Dana Farber Cancer Institute Assay methods and systems utilizing mast cell clones
GB8607679D0 (en) * 1986-03-27 1986-04-30 Winter G P Recombinant dna product
FR2621128B1 (fr) * 1987-09-30 1994-05-06 Sanofi Trousse et methode de dosage immunometrique applicables a des cellules entieres
GB8916859D0 (en) * 1989-07-24 1989-09-06 Dynal As Hapten linking

Also Published As

Publication number Publication date
ATE176930T1 (de) 1999-03-15
DE69323589D1 (de) 1999-04-01
DE69323589T2 (de) 1999-10-14
US5500348A (en) 1996-03-19
EP0596479A2 (en) 1994-05-11
KR100280239B1 (ko) 2001-02-01
JP3550410B2 (ja) 2004-08-04
JPH06205695A (ja) 1994-07-26
EP0596479B1 (en) 1999-02-24
KR940011480A (ko) 1994-06-21
DK0596479T3 (da) 1999-09-27
EP0596479A3 (en) 1995-04-19
TW378213B (en) 2000-01-01

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