ES2133104A1 - Un procedimiento para incrementar la produccion de penicilina en penicillium chrysogenum mediante la inactivacion del gen lys2. - Google Patents
Un procedimiento para incrementar la produccion de penicilina en penicillium chrysogenum mediante la inactivacion del gen lys2.Info
- Publication number
- ES2133104A1 ES2133104A1 ES09701343A ES9701343A ES2133104A1 ES 2133104 A1 ES2133104 A1 ES 2133104A1 ES 09701343 A ES09701343 A ES 09701343A ES 9701343 A ES9701343 A ES 9701343A ES 2133104 A1 ES2133104 A1 ES 2133104A1
- Authority
- ES
- Spain
- Prior art keywords
- gene
- lys2
- penicillin
- chrysogenum
- inactivation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000228150 Penicillium chrysogenum Species 0.000 title abstract 4
- 101150109301 lys2 gene Proteins 0.000 title abstract 4
- 229930182555 Penicillin Natural products 0.000 title abstract 3
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 title abstract 3
- 229940049954 penicillin Drugs 0.000 title abstract 3
- 230000002779 inactivation Effects 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 abstract 3
- 239000004472 Lysine Substances 0.000 abstract 2
- 238000009396 hybridization Methods 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 238000005215 recombination Methods 0.000 abstract 2
- 230000006798 recombination Effects 0.000 abstract 2
- 102100030981 Beta-alanine-activating enzyme Human genes 0.000 abstract 1
- 241000222122 Candida albicans Species 0.000 abstract 1
- 101000773364 Homo sapiens Beta-alanine-activating enzyme Proteins 0.000 abstract 1
- 101150062179 II gene Proteins 0.000 abstract 1
- 235000019766 L-Lysine Nutrition 0.000 abstract 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 abstract 1
- 108090000854 Oxidoreductases Proteins 0.000 abstract 1
- 102000004316 Oxidoreductases Human genes 0.000 abstract 1
- 108091007187 Reductases Proteins 0.000 abstract 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 abstract 1
- 241000235347 Schizosaccharomyces pombe Species 0.000 abstract 1
- 235000001014 amino acid Nutrition 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 abstract 1
- 229940095731 candida albicans Drugs 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 230000010354 integration Effects 0.000 abstract 1
- 235000018977 lysine Nutrition 0.000 abstract 1
- 239000003550 marker Substances 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 101150054232 pyrG gene Proteins 0.000 abstract 1
- 238000006467 substitution reaction Methods 0.000 abstract 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Un procedimiento para Incrementar la producción de penicilina en Penicillium chrysogenum mediante la inactivación del gen lys2. El gen lys2 de P. chrysogenum se ha identificado y aislado mediante hibridación con una sonda correspondiente al homólogo LYS2 de Saccharomyces cerevisiae. El gen de P. chrysogenum codifica una proteína de 1.296 aminoácidos y 142.421 Da con una identidad elevada con las reductasas de ácido a -aminoadípico de S. cerevisiae, Schizosaccharomyces pombe y Candida albicans. La disrupción del gen lys2 se ha realizado mediante (1) integración por recombinación simple y (II) sustitución génica por doble recombinación, empleando en ambos casos el gen pyrG como marcador de transformación. Los clones disrupcionados se han seleccionado como auxótrofos de lisina y confirmado por hibridación de Southern. Estos mutantes disrupcionados superproducen penicilina (40%-100% de Incremento con respecto a la cepa parental no disrupcionada) en un medio suplementado con L-lisinau4 mM. Figura 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES9701343A ES2133104B1 (es) | 1997-06-19 | 1997-06-19 | Un procedimiento para incrementar la produccion de penicilina en penicillium chrysogenum mediante la inactivacion del gen lys2. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES9701343A ES2133104B1 (es) | 1997-06-19 | 1997-06-19 | Un procedimiento para incrementar la produccion de penicilina en penicillium chrysogenum mediante la inactivacion del gen lys2. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2133104A1 true ES2133104A1 (es) | 1999-08-16 |
| ES2133104B1 ES2133104B1 (es) | 2000-04-01 |
Family
ID=8299742
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES9701343A Expired - Fee Related ES2133104B1 (es) | 1997-06-19 | 1997-06-19 | Un procedimiento para incrementar la produccion de penicilina en penicillium chrysogenum mediante la inactivacion del gen lys2. |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES2133104B1 (es) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1391510A4 (en) * | 2001-05-29 | 2005-06-01 | Asahi Glass Co Ltd | PROCESS FOR CONSTRUCTING A HOST AND MANUFACTURING PROCESS FOR FOREIGN PROTEIN |
-
1997
- 1997-06-19 ES ES9701343A patent/ES2133104B1/es not_active Expired - Fee Related
Non-Patent Citations (4)
| Title |
|---|
| BARNES, D.A. et al. "Genetic manipulation of Saccharomyces cerevisiae by use of the LYS2 gene". MOLECULAR AND CELLULAR BIOLOGY, 1986, Vol. 6, No 8, paginas 2828-2838, * |
| EIBEL, H. et al. "Identification of the cloned S. cerevisiae LYS2 gene by an integrative transformation approach". MOL. GEN. GENET., 1983, Vol. 191, No 1, paginas 66-73 * |
| HöNLINGER, C. et al. "Differential effects of general amino acid control of lysine biosynthesis on penicillin formation in strains of Penicillium chrysogenum". THE JOURNAL OF ANTIBIOTICS, 1988, Vol. 41, No 2, paginas 255-257 * |
| LU, Y. et al. "Regulation of alpha-aminoadipate reductase from Penicillium chrysogenum in relation to the flux from alpha-aminoadipate into penicillin biosynthesis". CAN. J. MICROBIOL., 1992, Vol. 38, paginas 758-763 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1391510A4 (en) * | 2001-05-29 | 2005-06-01 | Asahi Glass Co Ltd | PROCESS FOR CONSTRUCTING A HOST AND MANUFACTURING PROCESS FOR FOREIGN PROTEIN |
| US7723098B2 (en) | 2001-05-29 | 2010-05-25 | Asahi Glass Company, Limited | Method of constructing host and method of producing heterologous protein |
Also Published As
| Publication number | Publication date |
|---|---|
| ES2133104B1 (es) | 2000-04-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EC2A | Search report published |
Date of ref document: 19990816 Kind code of ref document: A1 Effective date: 19990816 |
|
| FD1A | Patent lapsed |
Effective date: 20050620 |