ES2136866T3 - Ensayos de captura. - Google Patents
Ensayos de captura.Info
- Publication number
- ES2136866T3 ES2136866T3 ES95925048T ES95925048T ES2136866T3 ES 2136866 T3 ES2136866 T3 ES 2136866T3 ES 95925048 T ES95925048 T ES 95925048T ES 95925048 T ES95925048 T ES 95925048T ES 2136866 T3 ES2136866 T3 ES 2136866T3
- Authority
- ES
- Spain
- Prior art keywords
- microorganism
- pct
- amount
- substrate
- solid substrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000000758 substrate Substances 0.000 abstract 5
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 abstract 4
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 abstract 4
- 230000003834 intracellular effect Effects 0.000 abstract 4
- 244000005700 microbiome Species 0.000 abstract 4
- 239000000463 material Substances 0.000 abstract 3
- 239000007787 solid Substances 0.000 abstract 3
- XTWYTFMLZFPYCI-KQYNXXCUSA-N 5'-adenylphosphoric acid Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O XTWYTFMLZFPYCI-KQYNXXCUSA-N 0.000 abstract 2
- XTWYTFMLZFPYCI-UHFFFAOYSA-N Adenosine diphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(O)=O)C(O)C1O XTWYTFMLZFPYCI-UHFFFAOYSA-N 0.000 abstract 2
- 102000002281 Adenylate kinase Human genes 0.000 abstract 2
- 108020000543 Adenylate kinase Proteins 0.000 abstract 2
- 239000011230 binding agent Substances 0.000 abstract 2
- 230000009870 specific binding Effects 0.000 abstract 2
- 230000027455 binding Effects 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Toxicology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Gyroscopes (AREA)
- Cereal-Derived Products (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
- Electrotherapy Devices (AREA)
- Water Treatment By Sorption (AREA)
- Steroid Compounds (AREA)
Abstract
SE PROPORCIONA UN METODO PARA DETERMINAR LA PRESENCIA Y/O CANTIDAD DE UN MICROORGANISMO Y/O SU MATERIAL INTRACELULAR PRESENTE EN UNA MUESTRA CARACTERIZADO EN QUE: (A) EXPOSICION DE LA MUESTRA A UN AGENTE DE UNION ESPECIFICO QUE HA SIDO INMOVILIZADO MEDIANTE UN SUSTRATO SOLIDO, EL AGENTE DE UNION ESPECIFICO SIENDO CAPAZ DE UNIRSE AL MICROORGANISMO A SU MATERIAL INTRACELULAR DE MODO QUE LLEGUE A ESTAR ASOCIADO CON EL SUSTRATO SOLIDO, (B) EXPOSICION DEL SUSTRATO SOLIDO A UN AGENTE CAPAZ DE HACER ACCESIBLE A CINASA DE ADENILATO ASOCIADO CON EL MICROORGANISMO Y/O SU MATERIAL INTRACELULAR A SOLUCIONES APLICADAS AL SUSTRATO, (C) APLICACION DE UNA SOLUCION QUE CONTIENE DIFOSFATO DE ADENOSINA (ADP) AL SUSTRATO BAJO CONDICIONES POR LAS QUE PUEDE PRODUCIRSE TRIFOSFATO DE ADENOSINA (ATP) MEDIANTE CUALQUIER CINASA DE ADENILATO PRESENTE, Y (D) MEDICION DE LA CANTIDAD DE TRIFOSFATO DE ADENOSINA (ATP) Y RELACION CON LA PRESENCIA Y/O CANTIDAD DE MICROORGANISMOS O CONTENIDO INTRACELULAR. EL PASO (D) PUEDE LLEVARSE A CABO MEDIANTE UN ENSAYO QUE INCLUYA UNA REACCION FORMADORA DE COLOR, AUNQUE ES PREFERIBLEMENTE LLEVADA A CABO MEDIANTE EL USO DE REACTIVO DE LUCIFERASA/LUCIFERINA PARA PRODUCIR LUZ PROPORCIONAR A LA CANTIDAD DE ATP PRODUCIDA, Y QUE ES DETECTADA MEDIANTE UN LUMINOMETRO. PREFERIBLEMENTE EL PASO (C) INCLUYE LA PRESENCIA DE IONES DE MAGNESIO EN UNA CONCENTRACION MOLAR SUFICIENTE PARA PERMITIR LA CONVERSION MAXIMA DE ADP EN ATP. EN CONCRETO EL PASO (B) Y EL PASO (C) SE LLEVAN A CABO MEDIANTE LA ADICION DE EXTRACTO, ADP E IONES DE MAGNESIO A LA MUESTRA E INCUBANDO LA MEZCLA DURANTE UN PERIODO PREDETERMINADO PARA EFECTUAR LA CONVERSION DE ADP EN ATP.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9414096A GB9414096D0 (en) | 1994-07-13 | 1994-07-13 | Labelled capture assay |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2136866T3 true ES2136866T3 (es) | 1999-12-01 |
Family
ID=10758240
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES95925048T Expired - Lifetime ES2136866T3 (es) | 1994-07-13 | 1995-07-12 | Ensayos de captura. |
Country Status (19)
| Country | Link |
|---|---|
| US (1) | US5798214A (es) |
| EP (1) | EP0774011B1 (es) |
| JP (1) | JP3589465B2 (es) |
| CN (1) | CN1090677C (es) |
| AT (1) | ATE184054T1 (es) |
| AU (1) | AU691940B2 (es) |
| BR (2) | BR9508392A (es) |
| CA (1) | CA2194458C (es) |
| DE (1) | DE69511876T2 (es) |
| DK (1) | DK0774011T3 (es) |
| ES (1) | ES2136866T3 (es) |
| GB (2) | GB9414096D0 (es) |
| GR (1) | GR3031877T3 (es) |
| HU (1) | HU220800B1 (es) |
| IN (1) | IN188668B (es) |
| NO (1) | NO316227B1 (es) |
| NZ (1) | NZ289464A (es) |
| WO (1) | WO1996002666A1 (es) |
| ZA (1) | ZA955846B (es) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9308411D0 (en) * | 1993-04-23 | 1993-06-09 | Celsis Ltd | Detection of biological material |
| US6569637B1 (en) | 1997-04-18 | 2003-05-27 | Kikkoman Corporation | Diagnostic reagents for renal function disorders and method for analyzing urine samples |
| EP1049798B1 (en) * | 1998-01-21 | 2006-06-14 | The Secretary of State for Defence | Antibiotic sensitivity testing |
| GB9801126D0 (en) * | 1998-01-21 | 1998-03-18 | Secr Defence | Antibiotic sensitivity testing |
| GB9803156D0 (en) * | 1998-02-13 | 1998-04-08 | Celsis Int Plc | Assay |
| WO2000039577A1 (en) * | 1998-12-24 | 2000-07-06 | Abb Instrumentation Limited | Contaminant detection |
| GB9902659D0 (en) | 1999-02-05 | 1999-03-31 | Microbiological Res Authority | Assay with reduced background |
| GB9911095D0 (en) * | 1999-05-13 | 1999-07-14 | Secr Defence | Microbiological test method and reagents |
| GB9923208D0 (en) * | 1999-10-01 | 1999-12-08 | Cambridge Drug Discovery Ltd | Assay |
| GB0010910D0 (en) * | 2000-05-05 | 2000-06-28 | Jones Osborn | Analytical method and apparatus |
| GB0122790D0 (en) * | 2001-09-21 | 2001-11-14 | Secr Defence | Method of determining the presence of target bacteria |
| US6750006B2 (en) * | 2002-01-22 | 2004-06-15 | Microbiosystems, Limited Partnership | Method for detecting the presence of microbes and determining their physiological status |
| EP1348757A1 (de) * | 2002-03-27 | 2003-10-01 | Micronas GmbH | Vorrichtung und Verfahren zur Detektion von zellulären Vorgängen mittels Lumineszenzmessungen |
| JPWO2003100086A1 (ja) * | 2002-05-23 | 2005-09-22 | 富士電機ホールディングス株式会社 | 生細胞の計数方法および装置 |
| CN1961077A (zh) * | 2004-06-01 | 2007-05-09 | 希森美康株式会社 | 酶活性测定方法及酶活性测定用柱 |
| US7422868B2 (en) * | 2004-07-02 | 2008-09-09 | Promega Corporation | Microbial ATP extraction and detection system |
| US7338775B1 (en) | 2005-02-09 | 2008-03-04 | Myriad Genetics, Inc. | Enzyme assay and use thereof |
| GB0606822D0 (en) * | 2006-04-05 | 2006-05-17 | Alaska Food Diagnostics | Assay System |
| US20080044880A1 (en) * | 2006-08-21 | 2008-02-21 | Samsung Electronics Co., Ltd. | Method of and apparatus for separating microorganisms from sample using electrodialysis and microorganism capturing means |
| JP2011504236A (ja) * | 2007-11-20 | 2011-02-03 | スリーエム イノベイティブ プロパティズ カンパニー | ジアセチレンを含むポリマーセンサーを用いる細菌試料の分析方法 |
| WO2009091402A1 (en) * | 2008-01-17 | 2009-07-23 | Gideon Eden | Co2 optical sensor for detection and enumeration of microorganisms |
| GB0900151D0 (en) * | 2009-01-07 | 2009-02-11 | Health Prot Agency | rapid bioluminescence detection system |
| US8518658B1 (en) * | 2009-04-27 | 2013-08-27 | University Of South Florida | ATP-bioluminescence immunoassay |
| US9932620B2 (en) | 2012-05-21 | 2018-04-03 | Celsis Ltd. | Methods, devices, and systems of detecting microorganisms |
| CN115667929A (zh) * | 2020-05-25 | 2023-01-31 | 横河电机株式会社 | 检体中的目标分子的检测方法以及目标分子检测试剂盒 |
| CN118703320A (zh) * | 2024-06-07 | 2024-09-27 | 西原环保(上海)股份有限公司 | 菌种丰度监测系统和方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3933592A (en) * | 1965-02-17 | 1976-01-20 | Hazleton Laboratories, Incorporated | Method of detecting living microorganisms |
| IT1172385B (it) * | 1983-12-21 | 1987-06-18 | Miles Italiana | Composizione e metodo per la determinazione enzimatica di atp ed fmn |
| US4794076A (en) * | 1985-09-23 | 1988-12-27 | Vxr, Inc. | Simultaneous extraction of a ligand from a sample and capture by anti-ligands therefor in ligand/anti-ligand assays |
| AU6847890A (en) * | 1990-01-19 | 1991-07-25 | Promega Corporation | Immunospecific and bioluminescent assay of cellular atp |
| JP2856339B2 (ja) * | 1991-02-21 | 1999-02-10 | キッコーマン株式会社 | 酵素免疫測定法 |
| US5648232A (en) * | 1993-01-21 | 1997-07-15 | The Secretary Of State For Defence In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland | Microbiological best method and reagents |
| GB9301118D0 (en) * | 1993-01-21 | 1993-03-10 | Secr Defence | Enzyme linked assays |
-
1994
- 1994-07-13 GB GB9414096A patent/GB9414096D0/en active Pending
-
1995
- 1995-07-12 BR BR9508392A patent/BR9508392A/pt active IP Right Grant
- 1995-07-12 CN CN95194994A patent/CN1090677C/zh not_active Expired - Fee Related
- 1995-07-12 JP JP50479696A patent/JP3589465B2/ja not_active Expired - Fee Related
- 1995-07-12 GB GB9700603A patent/GB2304892B/en not_active Revoked
- 1995-07-12 DK DK95925048T patent/DK0774011T3/da active
- 1995-07-12 WO PCT/GB1995/001643 patent/WO1996002666A1/en not_active Ceased
- 1995-07-12 AU AU29319/95A patent/AU691940B2/en not_active Ceased
- 1995-07-12 NZ NZ289464A patent/NZ289464A/en not_active IP Right Cessation
- 1995-07-12 DE DE69511876T patent/DE69511876T2/de not_active Expired - Lifetime
- 1995-07-12 BR BRPI9508392A patent/BRPI9508392B8/pt unknown
- 1995-07-12 EP EP95925048A patent/EP0774011B1/en not_active Expired - Lifetime
- 1995-07-12 HU HU9700090A patent/HU220800B1/hu not_active IP Right Cessation
- 1995-07-12 AT AT95925048T patent/ATE184054T1/de active
- 1995-07-12 ES ES95925048T patent/ES2136866T3/es not_active Expired - Lifetime
- 1995-07-12 CA CA002194458A patent/CA2194458C/en not_active Expired - Fee Related
- 1995-07-12 US US08/765,063 patent/US5798214A/en not_active Expired - Lifetime
- 1995-07-13 ZA ZA955846A patent/ZA955846B/xx unknown
- 1995-07-13 IN IN1315DE1995 patent/IN188668B/en unknown
-
1997
- 1997-01-10 NO NO19970106A patent/NO316227B1/no not_active IP Right Cessation
-
1999
- 1999-11-18 GR GR990402967T patent/GR3031877T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| BRPI9508392B8 (pt) | 2019-11-05 |
| NO970106D0 (no) | 1997-01-10 |
| NO316227B1 (no) | 2003-12-29 |
| IN188668B (es) | 2002-10-26 |
| CA2194458C (en) | 2007-09-11 |
| GR3031877T3 (en) | 2000-02-29 |
| GB2304892A (en) | 1997-03-26 |
| AU691940B2 (en) | 1998-05-28 |
| HU220800B1 (hu) | 2002-05-28 |
| CN1157638A (zh) | 1997-08-20 |
| WO1996002666A1 (en) | 1996-02-01 |
| AU2931995A (en) | 1996-02-16 |
| JPH10502815A (ja) | 1998-03-17 |
| HUT76447A (en) | 1997-09-29 |
| EP0774011A1 (en) | 1997-05-21 |
| GB9414096D0 (en) | 1994-08-31 |
| DK0774011T3 (da) | 2000-03-20 |
| DE69511876T2 (de) | 2000-05-31 |
| CN1090677C (zh) | 2002-09-11 |
| DE69511876D1 (de) | 1999-10-07 |
| JP3589465B2 (ja) | 2004-11-17 |
| EP0774011B1 (en) | 1999-09-01 |
| US5798214A (en) | 1998-08-25 |
| NO970106L (no) | 1997-03-13 |
| ZA955846B (en) | 1996-02-19 |
| NZ289464A (en) | 1998-07-28 |
| ATE184054T1 (de) | 1999-09-15 |
| CA2194458A1 (en) | 1996-02-01 |
| GB9700603D0 (en) | 1997-03-05 |
| GB2304892B (en) | 1998-08-19 |
| BR9508392A (pt) | 1998-05-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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