ES2139086T3 - El transposon tn5401 del bacillus thuringiensis y su uso en un sistema de recombinacion especifica de sitio para el desarrollo de cepas del bacillus thuringiensis. - Google Patents

El transposon tn5401 del bacillus thuringiensis y su uso en un sistema de recombinacion especifica de sitio para el desarrollo de cepas del bacillus thuringiensis.

Info

Publication number
ES2139086T3
ES2139086T3 ES94922547T ES94922547T ES2139086T3 ES 2139086 T3 ES2139086 T3 ES 2139086T3 ES 94922547 T ES94922547 T ES 94922547T ES 94922547 T ES94922547 T ES 94922547T ES 2139086 T3 ES2139086 T3 ES 2139086T3
Authority
ES
Spain
Prior art keywords
bacillus thuringiensis
transposon
specific recombination
strains
recombination system
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94922547T
Other languages
English (en)
Inventor
James A Baum
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ecogen Inc
Original Assignee
Ecogen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/089,986 external-priority patent/US5441884A/en
Application filed by Ecogen Inc filed Critical Ecogen Inc
Application granted granted Critical
Publication of ES2139086T3 publication Critical patent/ES2139086T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A01N63/23—B. thuringiensis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Dentistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Catching Or Destruction (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

UN ELEMENTO TRANSPORTABLE, O TRANSPOSON, AISLADO A PARTIR DE BACILLUS THURINGIENSIS (B.T.) Y DESIGNADO COMO TRANSPOSON TN5401. LA INVENCION TAMBIEN SE REFIERE A UN METODO DE USO DE ESTE TRANSPOSON EN UN SISTEMA DE RECOMBINACION ESPECIFICA LOCAL PARA LA CONSTRUCCION DE CEPAS DE B.T. RECOMBINANTES QUE CONTIENEN GENES DE PROTEINA DE TOXINA DE B.T. INSECTICIDA Y QUE ESTAN LIBRES DE ADN NO NATIVO EN B.T.
ES94922547T 1993-07-08 1994-07-08 El transposon tn5401 del bacillus thuringiensis y su uso en un sistema de recombinacion especifica de sitio para el desarrollo de cepas del bacillus thuringiensis. Expired - Lifetime ES2139086T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/089,986 US5441884A (en) 1993-07-08 1993-07-08 Bacillus thuringiensis transposon TN5401
US08/266,408 US5843744A (en) 1993-07-08 1994-06-24 Bacillus thuringiensis Tn5401 proteins

Publications (1)

Publication Number Publication Date
ES2139086T3 true ES2139086T3 (es) 2000-02-01

Family

ID=26781137

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94922547T Expired - Lifetime ES2139086T3 (es) 1993-07-08 1994-07-08 El transposon tn5401 del bacillus thuringiensis y su uso en un sistema de recombinacion especifica de sitio para el desarrollo de cepas del bacillus thuringiensis.

Country Status (9)

Country Link
US (2) US5843744A (es)
EP (1) EP0707654B1 (es)
JP (1) JPH11507801A (es)
AU (1) AU7361694A (es)
BR (1) BR9406881A (es)
CA (1) CA2166691C (es)
ES (1) ES2139086T3 (es)
GR (1) GR3032057T3 (es)
WO (1) WO1995002058A2 (es)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1177380A (zh) * 1995-01-23 1998-03-25 诺沃挪第克公司 通过转座的dna整合
US6964861B1 (en) * 1998-11-13 2005-11-15 Invitrogen Corporation Enhanced in vitro recombinational cloning of using ribosomal proteins
US6720140B1 (en) 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US6143557A (en) * 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
EP0937098B1 (en) 1995-06-07 2002-08-14 Invitrogen Corporation Recombinational cloning in vitro using engineered recombination sites
US5965428A (en) * 1996-10-08 1999-10-12 Ecogen, Inc. Chimeric lepidopteran-toxic crystal proteins
AU5432798A (en) 1996-11-08 1998-05-29 Morphogenesis, Inc. Materials and procedures for the purification of cells
US6713063B1 (en) 1996-11-20 2004-03-30 Monsanto Technology, Llc Broad-spectrum δ-endotoxins
JP4389075B2 (ja) 1996-11-20 2009-12-24 モンサント・テクノロジー・エルエルシー 広域スペクトルのデルタ−エンドトキシン
US6017534A (en) 1996-11-20 2000-01-25 Ecogen, Inc. Hybrid Bacillus thuringiensis δ-endotoxins with novel broad-spectrum insecticidal activity
US5942664A (en) 1996-11-27 1999-08-24 Ecogen, Inc. Bacillus thuringiensis Cry1C compositions toxic to lepidopteran insects and methods for making Cry1C mutants
US5851808A (en) * 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
US7351578B2 (en) * 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
DE69836092T2 (de) 1997-10-24 2007-05-10 Invitrogen Corp., Carlsbad Rekombinatorisches klonieren unter verwendung von nukleinsaüren mit rekombinationsstellen
WO2000052141A1 (en) * 1999-03-02 2000-09-08 Invitrogen Corporation Cells resistant to toxic genes and uses thereof
DE60042969D1 (de) * 1999-03-02 2009-10-29 Life Technologies Corp Zubereitungen und methoden zur verwendung in rekombinatorischem klonen von nukleinsäuren
US6890726B1 (en) 1999-04-06 2005-05-10 Oklahoma Medical Research Foundation Method for selecting recombinase variants with altered specificity
WO2000070019A2 (en) * 1999-05-17 2000-11-23 Icon Genetics, Inc. Process of rapid variety-independent plant transformation
ATE391789T1 (de) 1999-12-10 2008-04-15 Invitrogen Corp Verwendung einer vielzahl von rekombinationsstellen mit einzigartiger spezifität beim rekombinatorischen klonen
US7244560B2 (en) * 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7198924B2 (en) * 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US20030077804A1 (en) * 2001-04-19 2003-04-24 Invitrogen Corporation Compositions and methods for recombinational cloning of nucleic acid molecules
CA2448505A1 (en) * 2001-05-21 2002-11-28 Invitrogen Corporation Compositions and methods for use in isolation of nucleic acid molecules
WO2005028615A2 (en) * 2003-06-26 2005-03-31 Invitrogen Corporation Methods and compositions for detecting promoter activity and expressing fusion proteins
DE602004027538D1 (de) 2003-12-01 2010-07-15 Life Technologies Corp Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
CL2011002542A1 (es) * 2011-10-13 2012-03-02 Bio Insumos Nativa Spa Composicion insecticida biologica que comprende cepas de bacillus thuringiensis var. kurstaki; formulacion agricola; metodo de aplicacion; y uso para elaborar un agente insecticida para el control de plagas por insectos en plantas tales como tuta absoluta, proeulia spp, agrotis spp y spodoptera frugisperda.
WO2016050917A1 (en) * 2014-10-01 2016-04-07 Dsm Ip Assets B.V. Methods for genomic modification

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0200708B1 (en) * 1985-04-30 1992-12-30 Monsanto Company Plant-colonizing microorganisms containing the toxin gene of b. thuringiensis as a chromosomal insertion
CA1293460C (en) * 1985-10-07 1991-12-24 Brian Lee Sauer Site-specific recombination of dna in yeast
US5196342A (en) * 1987-04-16 1993-03-23 Prutech R&D Partnership Ii Bacillus thuringiensis P-2 toxin gene
US5024837A (en) * 1987-05-06 1991-06-18 Donovan William P Coleopteran active microorganisms, related insecticide compositions and methods for their production and use
US5080897A (en) * 1987-05-08 1992-01-14 Ecogen Inc. Novel bacillus thuringiensis strains, and related insecticidal compositions
ATE147431T1 (de) * 1988-05-20 1997-01-15 Ciba Geigy Ag Bacillus thuringiensis transformation
US5102797A (en) * 1989-05-26 1992-04-07 Dna Plant Technology Corporation Introduction of heterologous genes into bacteria using transposon flanked expression cassette and a binary vector system
US5188960A (en) * 1989-06-27 1993-02-23 Mycogen Corporation Bacillus thuringiensis isolate active against lepidopteran pests, and genes encoding novel lepidopteran-active toxins
US5187091A (en) * 1990-03-20 1993-02-16 Ecogen Inc. Bacillus thuringiensis cryiiic gene encoding toxic to coleopteran insects
ES2057897T3 (es) * 1990-05-15 1994-10-16 Ecogen Inc Vector lanzadera para el desarrollo de cepas de bacillus thuringiensis recombinantes-i.
WO1993001283A1 (en) * 1991-07-08 1993-01-21 The United States Of America As Represented By The Secretary Of Agriculture Selection-gene-free transgenic plants
FR2679568B1 (fr) * 1991-07-22 1994-09-02 Pasteur Institut Nouveau type de replicon gram-positif - construction de vecteurs recombinants le contenant.
EP0537105A1 (en) * 1991-07-26 1993-04-14 Sandoz Ltd. Vectors for bacillus thuringiensis
US5441884A (en) * 1993-07-08 1995-08-15 Ecogen Inc. Bacillus thuringiensis transposon TN5401

Also Published As

Publication number Publication date
GR3032057T3 (en) 2000-03-31
EP0707654A1 (en) 1996-04-24
AU7361694A (en) 1995-02-06
WO1995002058A3 (en) 1995-06-01
CA2166691C (en) 2000-11-21
JPH11507801A (ja) 1999-07-13
US5776449A (en) 1998-07-07
US5843744A (en) 1998-12-01
EP0707654B1 (en) 1999-09-15
BR9406881A (pt) 1996-04-02
CA2166691A1 (en) 1995-01-19
WO1995002058A2 (en) 1995-01-19

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