ES2142248B1 - Polimorfismos en el promotor del gen humano de la apolipoproteina e, y sus usos para aplicaciones diagnosticas y terapeuticas. - Google Patents
Polimorfismos en el promotor del gen humano de la apolipoproteina e, y sus usos para aplicaciones diagnosticas y terapeuticas.Info
- Publication number
- ES2142248B1 ES2142248B1 ES009702430A ES9702430A ES2142248B1 ES 2142248 B1 ES2142248 B1 ES 2142248B1 ES 009702430 A ES009702430 A ES 009702430A ES 9702430 A ES9702430 A ES 9702430A ES 2142248 B1 ES2142248 B1 ES 2142248B1
- Authority
- ES
- Spain
- Prior art keywords
- apolipoprotein
- dna
- gene
- genomic dna
- polymorphisms
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 101710095339 Apolipoprotein E Proteins 0.000 title abstract 4
- 102100029470 Apolipoprotein E Human genes 0.000 title abstract 2
- 102000054765 polymorphisms of proteins Human genes 0.000 title abstract 2
- 108090000623 proteins and genes Proteins 0.000 title abstract 2
- 230000001225 therapeutic effect Effects 0.000 title 1
- 238000000034 method Methods 0.000 abstract 4
- 108020004707 nucleic acids Proteins 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 108091008146 restriction endonucleases Proteins 0.000 abstract 2
- 208000024827 Alzheimer disease Diseases 0.000 abstract 1
- 230000004544 DNA amplification Effects 0.000 abstract 1
- 108091034117 Oligonucleotide Proteins 0.000 abstract 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 abstract 1
- 238000003149 assay kit Methods 0.000 abstract 1
- 208000035850 clinical syndrome Diseases 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 238000003745 diagnosis Methods 0.000 abstract 1
- 201000010099 disease Diseases 0.000 abstract 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 239000002751 oligonucleotide probe Substances 0.000 abstract 1
- 238000002560 therapeutic procedure Methods 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Método para detectar la presencia de polimorfismos de DNA, estuche de ensayo y ácidos nucleicos recombinantes correspondientes. El método consiste en analizar DNA genómico humano, DNA genómico clonado o DNA genómico amplificado mediante el empleo de moléculas de oligonucleótidos como sondas-que hibridan con una porción del gen de la apolipoproteína E. En otra variante, la detección se realiza mediante la digestión del DNA con una o varias enzimas de restricción, o bien mediante la utilización de diferentes técnicas electroforéticas. El estuche de ensayo se caracteriza por una cantidad definida de una o más sondas de oligonucleótidos marcados, una cantidad definida de una o más enzimas de restricción y medios necesarios para la amplificación del DNA. El ácido nucleico recombinante a utilizar en el método contiene cualquier fragmento del promotor del gen de la apolipoproteína E que incluye una serie de nucleótidos determinados del mencionado gen. La invención es aplicable a la diagnosis y terapia de la enferme dad de Alzheimer, enfermedad coronaria arteriosclerótica y otros síndromes clínicos asociados con la apolipoproteína E.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES009702430A ES2142248B1 (es) | 1997-11-20 | 1997-11-20 | Polimorfismos en el promotor del gen humano de la apolipoproteina e, y sus usos para aplicaciones diagnosticas y terapeuticas. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES009702430A ES2142248B1 (es) | 1997-11-20 | 1997-11-20 | Polimorfismos en el promotor del gen humano de la apolipoproteina e, y sus usos para aplicaciones diagnosticas y terapeuticas. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2142248A1 ES2142248A1 (es) | 2000-04-01 |
| ES2142248B1 true ES2142248B1 (es) | 2000-11-16 |
Family
ID=8301252
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES009702430A Expired - Fee Related ES2142248B1 (es) | 1997-11-20 | 1997-11-20 | Polimorfismos en el promotor del gen humano de la apolipoproteina e, y sus usos para aplicaciones diagnosticas y terapeuticas. |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES2142248B1 (es) |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU6546886A (en) * | 1985-09-30 | 1987-04-24 | Biotechnology Research Partners Limited | Polymorphisms related to lipid metabolism predictive of atherosclerosis: apob, apocii, apoe, apoaiv |
| JP3265577B2 (ja) * | 1992-10-13 | 2002-03-11 | デューク・ユニバーシティ | アポリポ蛋白ｅの４型イソ型の測定法 |
| CA2111503A1 (en) * | 1993-12-15 | 1995-06-16 | Mcgill University | Apolipoprotein e polymorphism and alzheimer's disease |
| GB9408465D0 (en) * | 1994-04-27 | 1994-06-22 | Univ Mcgill | Apolipoprotein e polymorphism & treatment of alzheimer's disease |
| ES2115553B1 (es) * | 1996-10-23 | 1999-02-16 | Boehringer Ingelheim Espana | Metodo para detectar la presencia de polimorfismos de dna, estuche de ensayo y acidos nucleicos recombinantes correspondientes. |
-
1997
- 1997-11-20 ES ES009702430A patent/ES2142248B1/es not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| ES2142248A1 (es) | 2000-04-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EC2A | Search report published |
Date of ref document: 20000401 Kind code of ref document: A1 Effective date: 20000401 |
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| FD2A | Announcement of lapse in spain |
Effective date: 20180227 |